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The map label for this gene is phbI [H]

Identifier: 161523623

GI number: 161523623

Start: 487206

End: 489089

Strand: Direct

Name: phbI [H]

Synonym: Bmul_0443

Alternate gene names: 161523623

Gene position: 487206-489089 (Clockwise)

Preceding gene: 161523622

Following gene: 161523627

Centisome position: 14.13

GC content: 65.71

Gene sequence:

>1884_bases
ATGCTGCGCTGCATGCAGTCAGCGGACACGCGCGGATTTATAATCGCTATCCGGGGTCATAAGCGCATCGCAGCAGTGTG
CCGCGGCTTTTCATGTACAGAGGAGGTGCGCGTGTCCTTCACGCTGCATGGCATTCCCGTCTCACGTGGTATCGCGATCG
GGCGAGCGTATCTGATCGCGCCGGCGGCGCTCGACGTCGCCCATTACCTGATCGAGGCGAACCGGATCGACGCCGAGGTC
GAGCGCTTTCGCACGGCGCTCGATGCCGTGCATCGCGAGCTCGAAGCACTGCGCGCCGACCTGACCGACGACACGCCGTC
CGAAGTCGGCGCATTCATCGACGTGCACGCGATGATCCTCGGCGACGCGATGCTCGTCCAGGAAACGATCGACCTGATCC
GCACGCGCCGCTACAACGTCGAATGGGCGTTGACCGAACAGCTCGATCTACTCACGCGCCACTTCGACGACATCGAGGAC
GAATACCTGCGCGAGCGCAAGGCCGACATCGAGCAAGTGGTCGAGCGCGTGCTGAAGGCGCTCGCGGGCGCACCGTCGGC
CGCCCAGGCGCTCGACGGCGCAGCGGCGAACGGCACGAGCGAGATGATCGTCGTCGCGCACGACATCGCGCCGGCCGACA
TGATGCAGTTCAAGACGCAGTCGTTCCAGGCGTTCGTCACCGATCTCGGCGGCCGCACGTCGCATACGGCGATCGTCGCG
CGCAGCCTCGGCATTCCGGCCGCGGTCGGCGTGCAGCACGCGAGCGCGCTGATCCGTCAGGACGATCTCATCATCGTCGA
CGGCGATCAGGGCATCGTGATCGTCGATCCGGCGCCAATCGTGCTCGAGGAATATTCGTATCGGCAGTCGGAGAAGCTGC
TCGAGCAGCGCAAGCTGCAGCGCCTGAAGTTCTCGCCGACGCAGACCTTGTGCGGCACGCCGATCGCGCTGTACGCGAAC
ATCGAGCTGCCCGACGATGCGAAGGCGGCGGTCGATGCGGGCGCCGTCGGCGTCGGGCTGTTTCGCTCCGAATTCCTGTT
CATGCATCAGAAGGAGATGCCGGAAGAGGAGGAGCAGTTCGCCGCGTACAAGCGTGCGGTCGAGTGGATGAAGGGCATGC
CCGTCACGATCCGCACGATCGACGTCGGCGCGGACAAGCCGCTCGAAGCGCTCGACGAAGGCTACGAAACCGCGCCGAAC
CCGGCGCTCGGGCTGCGCGCGATTCGCTGGAGTCTGTCCGAGCCGCAGATGTTCCTCACGCAGTTGCGCGCGATCCTGCG
CGCGTCGGCGTTCGGTCAGGTGAAGATCCTGATCCCGATGCTCGCGCATGCGCAGGAGATCGACCAGACGCTCGACCTGA
TTCGCGAAGCGAAGCGCCAGCTCGACGACGCGGGGCTCGCGTACGATCCGAACGTGCGCGTCGGTGCGATGATCGAGATT
CCGGCCGCCGCGATCGCGCTGCCGCTGTTCCTGAAGCGCTTCGATTTCCTGTCGATCGGCACGAACGACCTGATCCAGTA
CACGCTCGCGATCGATCGCGCCGACAACGCGGTCGCGCATCTGTACGATCCGCTGCATCCGGCCGTGCTGCACCTGATCG
CCTACACGCTGCGCGAAGCGAAGCGCGCGGGCGTGCCGGTGTCGGTGTGCGGCGAAATGGCCGGCGACCCGGCGCTCACG
CGGCTATTGCTCGGCATGGGGCTCACCGAATTCTCGATGCATCCGAGCCAGTTGCTCGTCGTGAAGCAGGAGATCCTGCG
TGCGCATCTGAAGTCGCTCGAAAAGCCGACGGCCGACGTGCTCGCCGCATTCGAGCCGGAGGAAGTGCAGGCGGCGTTGA
AGCGCCTCGCAGTCGCCGAACCGCGTGCGGACGCCGCCGCATAG

Upstream 100 bases:

>100_bases
AGGCCAGTAATCCGACGCACGGGATCGTTGCCGATTCCAGTCAGAATGCCGCGGATTCCGCGGCTTTTTTTTCGCCGGCA
GCATTGATGCGAAACGCGCA

Downstream 100 bases:

>100_bases
GCGTCAGCGACGAGTCCTCGACGATCATGACGATCGTTGCGCGCGCATGCGCGTCAGTGCCGCTTGCCGCACACCGGGCA
GTCGGGCTGGCGCGCGATCT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MLRCMQSADTRGFIIAIRGHKRIAAVCRGFSCTEEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEANRIDAEV
ERFRTALDAVHRELEALRADLTDDTPSEVGAFIDVHAMILGDAMLVQETIDLIRTRRYNVEWALTEQLDLLTRHFDDIED
EYLRERKADIEQVVERVLKALAGAPSAAQALDGAAANGTSEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVA
RSLGIPAAVGVQHASALIRQDDLIIVDGDQGIVIVDPAPIVLEEYSYRQSEKLLEQRKLQRLKFSPTQTLCGTPIALYAN
IELPDDAKAAVDAGAVGVGLFRSEFLFMHQKEMPEEEEQFAAYKRAVEWMKGMPVTIRTIDVGADKPLEALDEGYETAPN
PALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKILIPMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRVGAMIEI
PAAAIALPLFLKRFDFLSIGTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAYTLREAKRAGVPVSVCGEMAGDPALT
RLLLGMGLTEFSMHPSQLLVVKQEILRAHLKSLEKPTADVLAAFEPEEVQAALKRLAVAEPRADAAA

Sequences:

>Translated_627_residues
MLRCMQSADTRGFIIAIRGHKRIAAVCRGFSCTEEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEANRIDAEV
ERFRTALDAVHRELEALRADLTDDTPSEVGAFIDVHAMILGDAMLVQETIDLIRTRRYNVEWALTEQLDLLTRHFDDIED
EYLRERKADIEQVVERVLKALAGAPSAAQALDGAAANGTSEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVA
RSLGIPAAVGVQHASALIRQDDLIIVDGDQGIVIVDPAPIVLEEYSYRQSEKLLEQRKLQRLKFSPTQTLCGTPIALYAN
IELPDDAKAAVDAGAVGVGLFRSEFLFMHQKEMPEEEEQFAAYKRAVEWMKGMPVTIRTIDVGADKPLEALDEGYETAPN
PALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKILIPMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRVGAMIEI
PAAAIALPLFLKRFDFLSIGTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAYTLREAKRAGVPVSVCGEMAGDPALT
RLLLGMGLTEFSMHPSQLLVVKQEILRAHLKSLEKPTADVLAAFEPEEVQAALKRLAVAEPRADAAA
>Mature_627_residues
MLRCMQSADTRGFIIAIRGHKRIAAVCRGFSCTEEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEANRIDAEV
ERFRTALDAVHRELEALRADLTDDTPSEVGAFIDVHAMILGDAMLVQETIDLIRTRRYNVEWALTEQLDLLTRHFDDIED
EYLRERKADIEQVVERVLKALAGAPSAAQALDGAAANGTSEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVA
RSLGIPAAVGVQHASALIRQDDLIIVDGDQGIVIVDPAPIVLEEYSYRQSEKLLEQRKLQRLKFSPTQTLCGTPIALYAN
IELPDDAKAAVDAGAVGVGLFRSEFLFMHQKEMPEEEEQFAAYKRAVEWMKGMPVTIRTIDVGADKPLEALDEGYETAPN
PALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKILIPMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRVGAMIEI
PAAAIALPLFLKRFDFLSIGTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAYTLREAKRAGVPVSVCGEMAGDPALT
RLLLGMGLTEFSMHPSQLLVVKQEILRAHLKSLEKPTADVLAAFEPEEVQAALKRLAVAEPRADAAA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=568, Percent_Identity=37.8521126760563, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1789193, Length=537, Percent_Identity=33.8919925512104, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI48994992, Length=529, Percent_Identity=32.3251417769376, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1788726, Length=585, Percent_Identity=28.5470085470085, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1787994, Length=411, Percent_Identity=28.7104622871046, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI226510935, Length=218, Percent_Identity=25.6880733944954, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 68845; Mature: 68845

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRCMQSADTRGFIIAIRGHKRIAAVCRGFSCTEEVRVSFTLHGIPVSRGIAIGRAYLIA
CCCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCCEEEEEEEECCCHHCCHHHHHHHHHH
PAALDVAHYLIEANRIDAEVERFRTALDAVHRELEALRADLTDDTPSEVGAFIDVHAMIL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GDAMLVQETIDLIRTRRYNVEWALTEQLDLLTRHFDDIEDEYLRERKADIEQVVERVLKA
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAGAPSAAQALDGAAANGTSEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVA
HCCCCHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RSLGIPAAVGVQHASALIRQDDLIIVDGDQGIVIVDPAPIVLEEYSYRQSEKLLEQRKLQ
HHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHCCHHHHHHHHHHHHH
RLKFSPTQTLCGTPIALYANIELPDDAKAAVDAGAVGVGLFRSEFLFMHQKEMPEEEEQF
HHCCCCCCHHCCCCEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
AAYKRAVEWMKGMPVTIRTIDVGADKPLEALDEGYETAPNPALGLRAIRWSLSEPQMFLT
HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHH
QLRAILRASAFGQVKILIPMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRVGAMIEI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
PAAAIALPLFLKRFDFLSIGTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAYTLREA
CHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHH
KRAGVPVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKSLEKPTADV
HHCCCCHHHHHHCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
LAAFEPEEVQAALKRLAVAEPRADAAA
HHCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLRCMQSADTRGFIIAIRGHKRIAAVCRGFSCTEEVRVSFTLHGIPVSRGIAIGRAYLIA
CCCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCCEEEEEEEECCCHHCCHHHHHHHHHH
PAALDVAHYLIEANRIDAEVERFRTALDAVHRELEALRADLTDDTPSEVGAFIDVHAMIL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GDAMLVQETIDLIRTRRYNVEWALTEQLDLLTRHFDDIEDEYLRERKADIEQVVERVLKA
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAGAPSAAQALDGAAANGTSEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVA
HCCCCHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RSLGIPAAVGVQHASALIRQDDLIIVDGDQGIVIVDPAPIVLEEYSYRQSEKLLEQRKLQ
HHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHCCHHHHHHHHHHHHH
RLKFSPTQTLCGTPIALYANIELPDDAKAAVDAGAVGVGLFRSEFLFMHQKEMPEEEEQF
HHCCCCCCHHCCCCEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
AAYKRAVEWMKGMPVTIRTIDVGADKPLEALDEGYETAPNPALGLRAIRWSLSEPQMFLT
HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHH
QLRAILRASAFGQVKILIPMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRVGAMIEI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
PAAAIALPLFLKRFDFLSIGTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAYTLREA
CHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHH
KRAGVPVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKSLEKPTADV
HHCCCCHHHHHHCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
LAAFEPEEVQAALKRLAVAEPRADAAA
HHCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]