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The map label for this gene is amtB [H]

Identifier: 161523618

GI number: 161523618

Start: 482052

End: 483554

Strand: Direct

Name: amtB [H]

Synonym: Bmul_0438

Alternate gene names: 161523618

Gene position: 482052-483554 (Clockwise)

Preceding gene: 161523617

Following gene: 161523619

Centisome position: 13.98

GC content: 68.73

Gene sequence:

>1503_bases
ATGCGCAAAGTTCTGATGTCCCTGCTGATGGCCGGCTCGCTGATCGCGGCCGGTGTCGGCCCCGCGCTCGCGGACGACGC
GGCGTCCGCTGCGGCTGCGTCCGCGCCCGCCGCTTCGGCGCCCGACGCGTCGGCTGCCGCCGCACCCGCGGCCACGGCCT
CCGCCGCGCCCGACGCGTCCGCCGCCGCAGCCGCGCCGGCTTCGGCCGCCGCTTCGGCTCCCGCCGATGCATCGGCGGCC
GCCGCTCCCGCGGCGCCGACCGCGCCGTTCTCGGTCGATTCGTCGAAGATCAGCGCGGGCGACACCGCGTGGATGCTGAC
CTCCACCGCACTCGTGCTGTTCATGACGATCCCCGGCCTCGCGCTGTTCTACGCGGGCATGGTGCGCAAGAAGAACGTGC
TGGCGACCGTGATGCAGAGCTTCGCGATCACCGCGCTGATCACGGTGCTGTGGACTGTGATCGGCTACAGCCTCGCGTTC
ACGCCCGGCAACGGCTTCATCGGCGGCCTGTCGCGCGTGTTCCTGCACGGGATGAACTACATCAAGGGCGACAAGGCGAC
GACGCTGACCGTCAGCCACCTCGCGTCGACGATCCCGGAATCGGTCTACTTCGTCTACCAGATGACGTTCGCGATCATCA
CGCCGGCGCTGATCTGCGGCGCATTCGCGGACCGCATGAAGTTCTCGGCGATGCTCGTGTTCATGACGCTCTGGTCGCTG
ATCGTCTATGTGCCGATCGCGCACATGGTGTGGGAGCCGACCGGCTGGCTGTCGGCGGACGGCGTGCTCGACTTCGCGGG
CGGCACCGTGGTGCACATCAACGCCGGTATCGCGGGCCTGATGTCGTGCCTCGTGCTCGGCAAGCGTGTCGGCTACGGCC
GCGAATCGATGGCGCCGCACAACCTGGTGCTGACGATGATCGGCGGCTCGATGCTGTGGGTCGGCTGGTTCGGTTTCAAC
GCGGGCTCGGCGGTTGCCGCCGACGGCCGTGCCGGCTTCGCGATGCTGACGACGCAGGTCGCAACGGCGTGCGCGGCGCT
CGGCTGGATGTTTGCCGAATGGATCGCGAAGGGTAAGCCTTCGGTGCTCGGCATCGTGTCGGGTGCGGTCGCGGGGCTCG
TCGCGATCACGCCGGCGGCCGGCTTCGTCGGCGTGGCGGGTGCACTCGTGATCGGCATCGCCGCGGGCGTCGTCTGCTTC
TGGTCGGCGACCTGGCTGAAGTCGAAGCTCGGCTACGACGATTCGCTCGACGCGTTCGGCGTGCACGGCGTGGGCGGGAT
TCTCGGCGCACTGCTGACGGGCGTGTTCGCGGTCAAGGACATCGGCGGCTTCGACGGCAGCCTGCTGCTGCAGGCGAAGG
GCGTGCTGATCACGCTCGTCTACAGCGGCGTGCTGAGCTTCGTGCTGCTGAAGCTGATCGACCTGACGATCGGCCTGCGC
GTGACCGAAGAGGAAGAGCGCGAAGGCCTCGACGTGATCCTGCACGGCGAACACGTCGAATAA

Upstream 100 bases:

>100_bases
TCTTCGTCACGCCGATCGAGCAGGTGATCCGGATTCGGACCGGGGAGACGGGCGCCGACGCGCTGTAACAAACAAGACGA
GCGACAAGAGGAAACCTAAG

Downstream 100 bases:

>100_bases
GCGCACCCCATCCGGCGGGGCGCCGCGACGGCGGCCCGCCGCACACGATGAGCGCAGCGACTTCGGCCCGCCTTCCGGCG
GGCTTTTTTTCTCCGCACGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MRKVLMSLLMAGSLIAAGVGPALADDAASAAAASAPAASAPDASAAAAPAATASAAPDASAAAAAPASAAASAPADASAA
AAPAAPTAPFSVDSSKISAGDTAWMLTSTALVLFMTIPGLALFYAGMVRKKNVLATVMQSFAITALITVLWTVIGYSLAF
TPGNGFIGGLSRVFLHGMNYIKGDKATTLTVSHLASTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSL
IVYVPIAHMVWEPTGWLSADGVLDFAGGTVVHINAGIAGLMSCLVLGKRVGYGRESMAPHNLVLTMIGGSMLWVGWFGFN
AGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALVIGIAAGVVCF
WSATWLKSKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGFDGSLLLQAKGVLITLVYSGVLSFVLLKLIDLTIGLR
VTEEEEREGLDVILHGEHVE

Sequences:

>Translated_500_residues
MRKVLMSLLMAGSLIAAGVGPALADDAASAAAASAPAASAPDASAAAAPAATASAAPDASAAAAAPASAAASAPADASAA
AAPAAPTAPFSVDSSKISAGDTAWMLTSTALVLFMTIPGLALFYAGMVRKKNVLATVMQSFAITALITVLWTVIGYSLAF
TPGNGFIGGLSRVFLHGMNYIKGDKATTLTVSHLASTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSL
IVYVPIAHMVWEPTGWLSADGVLDFAGGTVVHINAGIAGLMSCLVLGKRVGYGRESMAPHNLVLTMIGGSMLWVGWFGFN
AGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALVIGIAAGVVCF
WSATWLKSKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGFDGSLLLQAKGVLITLVYSGVLSFVLLKLIDLTIGLR
VTEEEEREGLDVILHGEHVE
>Mature_500_residues
MRKVLMSLLMAGSLIAAGVGPALADDAASAAAASAPAASAPDASAAAAPAATASAAPDASAAAAAPASAAASAPADASAA
AAPAAPTAPFSVDSSKISAGDTAWMLTSTALVLFMTIPGLALFYAGMVRKKNVLATVMQSFAITALITVLWTVIGYSLAF
TPGNGFIGGLSRVFLHGMNYIKGDKATTLTVSHLASTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSL
IVYVPIAHMVWEPTGWLSADGVLDFAGGTVVHINAGIAGLMSCLVLGKRVGYGRESMAPHNLVLTMIGGSMLWVGWFGFN
AGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALVIGIAAGVVCF
WSATWLKSKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGFDGSLLLQAKGVLITLVYSGVLSFVLLKLIDLTIGLR
VTEEEEREGLDVILHGEHVE

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=406, Percent_Identity=50.4926108374384, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17550182, Length=452, Percent_Identity=28.7610619469027, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=26.3157894736842, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=434, Percent_Identity=26.9585253456221, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=453, Percent_Identity=24.7240618101545, Blast_Score=99, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=36.046511627907, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6325396, Length=428, Percent_Identity=34.5794392523364, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=32.2966507177034, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI161078314, Length=421, Percent_Identity=27.5534441805226, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50743; Mature: 50743

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVLMSLLMAGSLIAAGVGPALADDAASAAAASAPAASAPDASAAAAPAATASAAPDAS
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCC
AAAAAPASAAASAPADASAAAAPAAPTAPFSVDSSKISAGDTAWMLTSTALVLFMTIPGL
HHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALFYAGMVRKKNVLATVMQSFAITALITVLWTVIGYSLAFTPGNGFIGGLSRVFLHGMNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCC
IKGDKATTLTVSHLASTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSL
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYVPIAHMVWEPTGWLSADGVLDFAGGTVVHINAGIAGLMSCLVLGKRVGYGRESMAPH
HHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCC
NLVLTMIGGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIAKGKP
CEEEEEECCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SVLGIVSGAVAGLVAITPAAGFVGVAGALVIGIAAGVVCFWSATWLKSKLGYDDSLDAFG
CEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VHGVGGILGALLTGVFAVKDIGGFDGSLLLQAKGVLITLVYSGVLSFVLLKLIDLTIGLR
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCE
VTEEEEREGLDVILHGEHVE
ECCHHHHCCCEEEEECCCCC
>Mature Secondary Structure
MRKVLMSLLMAGSLIAAGVGPALADDAASAAAASAPAASAPDASAAAAPAATASAAPDAS
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCC
AAAAAPASAAASAPADASAAAAPAAPTAPFSVDSSKISAGDTAWMLTSTALVLFMTIPGL
HHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALFYAGMVRKKNVLATVMQSFAITALITVLWTVIGYSLAFTPGNGFIGGLSRVFLHGMNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCC
IKGDKATTLTVSHLASTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSL
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYVPIAHMVWEPTGWLSADGVLDFAGGTVVHINAGIAGLMSCLVLGKRVGYGRESMAPH
HHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCC
NLVLTMIGGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIAKGKP
CEEEEEECCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SVLGIVSGAVAGLVAITPAAGFVGVAGALVIGIAAGVVCFWSATWLKSKLGYDDSLDAFG
CEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VHGVGGILGALLTGVFAVKDIGGFDGSLLLQAKGVLITLVYSGVLSFVLLKLIDLTIGLR
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCE
VTEEEEREGLDVILHGEHVE
ECCHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]