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The map label for this gene is dctD [H]

Identifier: 161523612

GI number: 161523612

Start: 476219

End: 477571

Strand: Direct

Name: dctD [H]

Synonym: Bmul_0432

Alternate gene names: 161523612

Gene position: 476219-477571 (Clockwise)

Preceding gene: 161523611

Following gene: 161523614

Centisome position: 13.81

GC content: 70.36

Gene sequence:

>1353_bases
ATGGCCAACCGGCTGCAAGTGATCTACATCGAAGACGACGCGCTCGTTCGCCGGGCGAGCGTGCAGAGCCTGCAACTCGC
GGGCTTCGACGTCGCCGGCTTCGAATCGGCCGAGGCGGCCGACAAGGCGATCGTCGCCGACAACGCCGGCGTGATCGTCA
GCGACATCCGGCTGCCCGGCGCGAGCGGGCTCGACCTGCTTGCGCAATGCCGCGCGCGCGTGCCCGACGTGCCGGTGATT
CTCGTCACCGGTCACGGCGACATCTCGATGGCCGTGCAGGCGATGCGCGACGGCGCATACGACTTCATCGAGAAGCCGTT
CGCGGCCGAGCGGCTGATCGAGACCGTGCGGCGCGCGCTCGAGCGACGCGAGCTCGTGCTCGAGAATCACGCGCTGCGGC
GCGAACTGGCCGGCCAGAACGTCGTCGCGCCGCGCATCATCGGCCGCAGCCCGGCGATCGAGCAGGTGCGCAAGCTGATC
GCCAACATCGCGCCGACCGACGCGTCGGTGCTGATCAACGGCGACACGGGCGCCGGCAAGGAGCTGATCGCACGCAGCCT
GCACGAGCTGTCGCCGCGCCGCGACAAGCCGTTCATCGCGGTGAACTGCGGCGCGCTGCCCGAGCCGATGTTCGAGTCCG
AGATGTTCGGCTACGAGCCCGGCGCGTTCACCGGCGCCGCGAAGCGCCGCATCGGCAAGCTCGAATACGCGTCCGGCGGC
ACGCTGTTTCTCGACGAAATCGAGAGCATGCCGCTCGCGTTGCAGGTGAAGCTGCTGCGCGTGCTGCAGGACGGCGTGCT
CGAACGGCTCGGCTCGAACCAGCCGATCCGCGTGAATTGCCGTGTCGTCGCGGCGGCGAAGGGCGACATGAGCGAGCTCG
TCGCGGCCGGCACGTTCCGCCGCGACCTGCTGTATCGGCTGAACGTCGTGACGATCGCGCTGCCGCCGCTCGCCGAGCGC
CGCGAGGATATCGTGCCGCTGTTCGAGCACTTCATGCTCGATGCGGCCGTGCGCTACGGGCGGCCCGCGCCGGTGCTGAG
CGACCGGCAGCGCGCGAGCCTGATGCAGCGCGACTGGCCCGGCAACGTGCGCGAGCTGCGCAACGCGGCCGACCGCTTCG
TGCTGGGCGTCGCCGAGATGCCGGCCGAGGCCGACGTGGCCGGCGATGCGGCGAGCGAGCAGACGCTGAAGGAGCGCGTC
GAGCAGTTCGAGCGTGCGGTGATCGCCGAGGCGCTGAACCAGACGGGCGGCGCCGTCGCGGCGACCGCCGACCGGCTGCA
CGTCGGCAAGGCGACGCTGTACGAGAAGATGAAGCGCTACGGGCTGTCCGCGAAAGGCGAATCGGAGCGCTGA

Upstream 100 bases:

>100_bases
GCCTGCAGTGAGCGCGTGCGCGCTGCGCGTCCGGCGCCCGGCGGCATCAACGACGCCGCGCGCGCGACGGCGCGTGCTGC
GCGAGGAGTGAGTGAGTACG

Downstream 100 bases:

>100_bases
AATGAAAACGGACCGCGGAACCGGCTGTGCCGGCGATGCGGTCCGTGTGGGGCGCGGCGCGCGGCGGCGCTTACGCCGCG
TTGCCGTCGAGCGCCTTCTT

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MANRLQVIYIEDDALVRRASVQSLQLAGFDVAGFESAEAADKAIVADNAGVIVSDIRLPGASGLDLLAQCRARVPDVPVI
LVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRALERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLI
ANIAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYGRPAPVLSDRQRASLMQRDWPGNVRELRNAADRFVLGVAEMPAEADVAGDAASEQTLKERV
EQFERAVIAEALNQTGGAVAATADRLHVGKATLYEKMKRYGLSAKGESER

Sequences:

>Translated_450_residues
MANRLQVIYIEDDALVRRASVQSLQLAGFDVAGFESAEAADKAIVADNAGVIVSDIRLPGASGLDLLAQCRARVPDVPVI
LVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRALERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLI
ANIAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYGRPAPVLSDRQRASLMQRDWPGNVRELRNAADRFVLGVAEMPAEADVAGDAASEQTLKERV
EQFERAVIAEALNQTGGAVAATADRLHVGKATLYEKMKRYGLSAKGESER
>Mature_449_residues
ANRLQVIYIEDDALVRRASVQSLQLAGFDVAGFESAEAADKAIVADNAGVIVSDIRLPGASGLDLLAQCRARVPDVPVIL
VTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRALERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLIA
NIAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGGT
LFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFRRDLLYRLNVVTIALPPLAERR
EDIVPLFEHFMLDAAVRYGRPAPVLSDRQRASLMQRDWPGNVRELRNAADRFVLGVAEMPAEADVAGDAASEQTLKERVE
QFERAVIAEALNQTGGAVAATADRLHVGKATLYEKMKRYGLSAKGESER

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=38.936170212766, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=38.2743362831858, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=38.0952380952381, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1788905, Length=450, Percent_Identity=36.8888888888889, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI87082117, Length=339, Percent_Identity=41.8879056047198, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1789087, Length=348, Percent_Identity=41.6666666666667, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=40, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI87082152, Length=318, Percent_Identity=42.7672955974843, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1786524, Length=360, Percent_Identity=38.0555555555556, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87081872, Length=239, Percent_Identity=46.0251046025105, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=37.3860182370821, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87081858, Length=377, Percent_Identity=29.4429708222812, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=35.5932203389831, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=28.4552845528455, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790552, Length=119, Percent_Identity=27.7310924369748, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49048; Mature: 48917

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRLQVIYIEDDALVRRASVQSLQLAGFDVAGFESAEAADKAIVADNAGVIVSDIRLPG
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEECCCCEEEEEEECCC
ASGLDLLAQCRARVPDVPVILVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRAL
CCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLIANIAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPVLSDRQRASLMQRDWP
HHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
GNVRELRNAADRFVLGVAEMPAEADVAGDAASEQTLKERVEQFERAVIAEALNQTGGAVA
CCHHHHHHHHHHHEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ATADRLHVGKATLYEKMKRYGLSAKGESER
EEHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANRLQVIYIEDDALVRRASVQSLQLAGFDVAGFESAEAADKAIVADNAGVIVSDIRLPG
CCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEECCCCEEEEEEECCC
ASGLDLLAQCRARVPDVPVILVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRAL
CCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLIANIAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPVLSDRQRASLMQRDWP
HHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
GNVRELRNAADRFVLGVAEMPAEADVAGDAASEQTLKERVEQFERAVIAEALNQTGGAVA
CCHHHHHHHHHHHEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ATADRLHVGKATLYEKMKRYGLSAKGESER
EEHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]