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The map label for this gene is dctA [H]

Identifier: 161523610

GI number: 161523610

Start: 472829

End: 474118

Strand: Direct

Name: dctA [H]

Synonym: Bmul_0430

Alternate gene names: 161523610

Gene position: 472829-474118 (Clockwise)

Preceding gene: 161523609

Following gene: 161523611

Centisome position: 13.71

GC content: 64.26

Gene sequence:

>1290_bases
GTGAAGAAGAAACCCTTCTACAAAGTGCTTTATGTGCAGGTGATCTTCGCCATCGTCGTCGGCGTGATCCTCGGTCACTA
CTATCCGTCGCTCGCCACCGAGATGAAACCGCTCGGCGACGGGTTCATCAAGCTGATCAAGATGGTGATCGGTCCGATCA
TCTTCTGTACGGTCGTCACCGGCATCGCCGGGATGGAGGACATGAAGAAGGTCGGCCGCGTCGGCGGCAAGGCGCTGCTG
TACTTCGAGATCGTGTCGACGTTCGCGCTCGTGCTCGGTCTCGCGGCGACGCACATCCTGCGTCCGGGCGTCGGCTTCAA
CATCGATCCGGCGACGCTCGACGGCAAGGCCGTCGCGTCGTACGCGGCGAAGGCGCACGGCCAGTCGACGGTCGACTTCC
TGATGCACATCATCCCGAACACGATGGTCGACGCGTTCGCGCAGGGCGAGATCCTGCAGATCCTGCTGATTGCGCTGCTG
TTCGGCAGCGTGCTCGCGCACCTCGGCGAGCGCGGCAAGGTCGTCACGGACTTCATCGACGGCCTCACGCGCGTGCTGTT
CGGCATCGTGCACATCGTCACGAAGCTCGCACCGATCGGCGCGTTCGGCGCGATGGCATTCACGATCGGCAAGTACGGCG
TCGGCTCGCTGGTGCCGCTGCTGAAGCTGATCGGCACGTTCTACCTGACCTCGGTCGTGTTCGTGCTCGTCGTGCTCGGC
GCGATCGCGCGCTTCACCGGCTTCTCGATCATCCGCTTCGTGTCCTACATCAAGGAAGAGCTGCTGATCGTGCTCGGCAC
GAGCTCGTCGGAAGCCGCGCTGCCGCAGCTGATGGAAAAGCTCGAGAAGGCCGGCTGCTCGCGCTCGGTCGTCGGCCTCG
TCGTGCCGACCGGCTATTCGTTCAACCTCGACGGCACCAACATCTACATGACGATGGCCGTGCTGTTCATCGCGCAGGCG
ACCAACATCGAACTGACCTGGATGCAGCAGCTCACGCTGCTCGCGGTCGCGATGCTGACCTCGAAGGGCGCGAGCGGCGT
CACGGGCGCGGGCTTCATCACGCTCGCCGCGACGCTTGCCGTCGTGCCGACGATTCCGCTGTCGGGCATGGTGCTGATCC
TCGGCATCGATCGCTTCATGAGCGAATGCCGCGCGCTGACGAACATCGTCGGCAACGGCGTCGCGACCGTCGTCGTGTCG
GCATGGGAAAAGGAACTCGATCGTGCGAAGCTGCGCCAGGCGCTGAAGGGCGGCGGCGAAGTCGCGGCGACCGAGACGGC
CGGCGTCTGA

Upstream 100 bases:

>100_bases
GCGCGGGCGCGTGCCGCGGCGGGCATTGCCCTCCGTCCTCGTCCCTTTCCGCGCGCGTGACGCCGTTTCCCCCGGCTCAT
CTACTTCAGCAGGAACCATC

Downstream 100 bases:

>100_bases
TGCGATGAACGGGCAGGCGGCCGCGCCGTCCGCGCGCGCCGCGCCGCGCGGCGGCGATCGCGCTCGGGACGCGTATGACA
CAATAGGCGATCCGCATCGC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKKKPFYKVLYVQVIFAIVVGVILGHYYPSLATEMKPLGDGFIKLIKMVIGPIIFCTVVTGIAGMEDMKKVGRVGGKALL
YFEIVSTFALVLGLAATHILRPGVGFNIDPATLDGKAVASYAAKAHGQSTVDFLMHIIPNTMVDAFAQGEILQILLIALL
FGSVLAHLGERGKVVTDFIDGLTRVLFGIVHIVTKLAPIGAFGAMAFTIGKYGVGSLVPLLKLIGTFYLTSVVFVLVVLG
AIARFTGFSIIRFVSYIKEELLIVLGTSSSEAALPQLMEKLEKAGCSRSVVGLVVPTGYSFNLDGTNIYMTMAVLFIAQA
TNIELTWMQQLTLLAVAMLTSKGASGVTGAGFITLAATLAVVPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVS
AWEKELDRAKLRQALKGGGEVAATETAGV

Sequences:

>Translated_429_residues
MKKKPFYKVLYVQVIFAIVVGVILGHYYPSLATEMKPLGDGFIKLIKMVIGPIIFCTVVTGIAGMEDMKKVGRVGGKALL
YFEIVSTFALVLGLAATHILRPGVGFNIDPATLDGKAVASYAAKAHGQSTVDFLMHIIPNTMVDAFAQGEILQILLIALL
FGSVLAHLGERGKVVTDFIDGLTRVLFGIVHIVTKLAPIGAFGAMAFTIGKYGVGSLVPLLKLIGTFYLTSVVFVLVVLG
AIARFTGFSIIRFVSYIKEELLIVLGTSSSEAALPQLMEKLEKAGCSRSVVGLVVPTGYSFNLDGTNIYMTMAVLFIAQA
TNIELTWMQQLTLLAVAMLTSKGASGVTGAGFITLAATLAVVPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVS
AWEKELDRAKLRQALKGGGEVAATETAGV
>Mature_429_residues
MKKKPFYKVLYVQVIFAIVVGVILGHYYPSLATEMKPLGDGFIKLIKMVIGPIIFCTVVTGIAGMEDMKKVGRVGGKALL
YFEIVSTFALVLGLAATHILRPGVGFNIDPATLDGKAVASYAAKAHGQSTVDFLMHIIPNTMVDAFAQGEILQILLIALL
FGSVLAHLGERGKVVTDFIDGLTRVLFGIVHIVTKLAPIGAFGAMAFTIGKYGVGSLVPLLKLIGTFYLTSVVFVLVVLG
AIARFTGFSIIRFVSYIKEELLIVLGTSSSEAALPQLMEKLEKAGCSRSVVGLVVPTGYSFNLDGTNIYMTMAVLFIAQA
TNIELTWMQQLTLLAVAMLTSKGASGVTGAGFITLAATLAVVPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVS
AWEKELDRAKLRQALKGGGEVAATETAGV

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=478, Percent_Identity=25.3138075313808, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI4827012, Length=274, Percent_Identity=30.2919708029197, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI21314632, Length=400, Percent_Identity=25.5, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI66773030, Length=404, Percent_Identity=24.7524752475248, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI194239697, Length=460, Percent_Identity=24.5652173913043, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI5032093, Length=396, Percent_Identity=27.020202020202, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI262359914, Length=418, Percent_Identity=24.4019138755981, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI223468564, Length=268, Percent_Identity=29.1044776119403, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI223468566, Length=268, Percent_Identity=29.1044776119403, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI40254478, Length=277, Percent_Identity=25.9927797833935, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI301601644, Length=166, Percent_Identity=30.1204819277108, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1789947, Length=415, Percent_Identity=62.1686746987952, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1790514, Length=411, Percent_Identity=38.9294403892944, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788024, Length=398, Percent_Identity=21.8592964824121, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789473, Length=323, Percent_Identity=25.077399380805, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=436, Percent_Identity=27.2935779816514, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI193206505, Length=475, Percent_Identity=24.8421052631579, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71996953, Length=486, Percent_Identity=24.2798353909465, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71983106, Length=431, Percent_Identity=24.3619489559165, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71983099, Length=431, Percent_Identity=24.3619489559165, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI193206654, Length=430, Percent_Identity=23.0232558139535, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=395, Percent_Identity=22.5316455696203, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583025, Length=408, Percent_Identity=23.2843137254902, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137668, Length=408, Percent_Identity=23.2843137254902, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24583023, Length=408, Percent_Identity=23.2843137254902, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281360481, Length=429, Percent_Identity=25.1748251748252, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281360483, Length=429, Percent_Identity=25.1748251748252, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137666, Length=429, Percent_Identity=25.1748251748252, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45440; Mature: 45440

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EECCCCCCC
>Mature Secondary Structure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EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA