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The map label for this gene is hldE [H]

Identifier: 161523577

GI number: 161523577

Start: 439262

End: 439744

Strand: Direct

Name: hldE [H]

Synonym: Bmul_0397

Alternate gene names: 161523577

Gene position: 439262-439744 (Clockwise)

Preceding gene: 161523576

Following gene: 161523580

Centisome position: 12.74

GC content: 66.87

Gene sequence:

>483_bases
ATGCCCGCTTCCTTCGAACGCAAGCTGATCACCCGCGACGCACTCGTCGCGCTGCGCGCATCGCTGCCGTCGCCCGTCGT
CTTCACGAACGGCGTGTTCGACATCCTGCATCGCGGCCACGTCACCTATCTCGCCGATGCGAAGGCGCTCGGCGCGTGCC
TGATCGTCGGCGTGAACAGCGACGCGTCGGTGCGCATGCTCGGCAAGGGCGACGACCGCCCGATTAACCGCGAAGACGAC
CGGATGGCGCTGCTCGCGGCGCTGGAAAGCGTCGACTGGGTCGTGAAGTTCGAGGAAAAGACGCCGGTGTCGCTGATCGA
AGCGGTTCATCCGGACATCCTCGTGAAGGGCGGCGACTACGATATGGACGCGCTGCCCGAATCGGCGCTCGTGCGCGGCT
GGGGCGGCCGCGCGCTCGCGATTCCGTTCGAGCACGATCGCTCGACGACCGCGCTGTTGAAGAAGGTGCGCGCGCAGCAG
TGA

Upstream 100 bases:

>100_bases
CCCGACGGGCTTTTTCTCTGGCATCGCGCGGGCCGGTCCCGTCTGCCGCGCGCAGGACGTCCCGCTCGATCCGTTCCATC
CCATCCGCCCGACTTTCATC

Downstream 100 bases:

>100_bases
CGCCCGCGCGCTAGCGCGCCGACGACGCGGCCAGCGGCTCGGCCGCCACGGCCGGCGGCGCGATCGGCCCGCCGACGACC
GGTTGATCGGTCGCTTGCGC

Product: rfaE bifunctional protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MPASFERKLITRDALVALRASLPSPVVFTNGVFDILHRGHVTYLADAKALGACLIVGVNSDASVRMLGKGDDRPINREDD
RMALLAALESVDWVVKFEEKTPVSLIEAVHPDILVKGGDYDMDALPESALVRGWGGRALAIPFEHDRSTTALLKKVRAQQ

Sequences:

>Translated_160_residues
MPASFERKLITRDALVALRASLPSPVVFTNGVFDILHRGHVTYLADAKALGACLIVGVNSDASVRMLGKGDDRPINREDD
RMALLAALESVDWVVKFEEKTPVSLIEAVHPDILVKGGDYDMDALPESALVRGWGGRALAIPFEHDRSTTALLKKVRAQQ
>Mature_159_residues
PASFERKLITRDALVALRASLPSPVVFTNGVFDILHRGHVTYLADAKALGACLIVGVNSDASVRMLGKGDDRPINREDDR
MALLAALESVDWVVKFEEKTPVSLIEAVHPDILVKGGDYDMDALPESALVRGWGGRALAIPFEHDRSTTALLKKVRAQQ

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=132, Percent_Identity=56.8181818181818, Blast_Score=144, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.- [C]

Molecular weight: Translated: 17445; Mature: 17314

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASFERKLITRDALVALRASLPSPVVFTNGVFDILHRGHVTYLADAKALGACLIVGVNS
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEEEEECCCCCCEEEEEEECC
DASVRMLGKGDDRPINREDDRMALLAALESVDWVVKFEEKTPVSLIEAVHPDILVKGGDY
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHCCCCEEEECCCC
DMDALPESALVRGWGGRALAIPFEHDRSTTALLKKVRAQQ
CHHHCCCHHHEECCCCCEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PASFERKLITRDALVALRASLPSPVVFTNGVFDILHRGHVTYLADAKALGACLIVGVNS
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEEEEECCCCCCEEEEEEECC
DASVRMLGKGDDRPINREDDRMALLAALESVDWVVKFEEKTPVSLIEAVHPDILVKGGDY
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHCCCCEEEECCCC
DMDALPESALVRGWGGRALAIPFEHDRSTTALLKKVRAQQ
CHHHCCCHHHEECCCCCEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500908 [H]