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The map label for this gene is mlaE [H]

Identifier: 161523498

GI number: 161523498

Start: 351779

End: 352546

Strand: Direct

Name: mlaE [H]

Synonym: Bmul_0318

Alternate gene names: 161523498

Gene position: 351779-352546 (Clockwise)

Preceding gene: 161523497

Following gene: 161523499

Centisome position: 10.2

GC content: 65.89

Gene sequence:

>768_bases
ATGATCAGCGCGATCGGACGTTACGTCATCGGCGGCCTCGAGCGCGCGGGCTACGGCACGCGGCTGTTCGTGCGCCTCGT
GCTCGAATTCTTCCCGCTGCTGCGCCGGCCGCGGCTCGTCACGAAGCAGATCCACTTCCTCGGCAACTATTCGTTCGTGA
TCATCGCCGTGTCGGGGCTGTTCGTCGGCTTCGTGCTCGGGCTGCAGGGCTATTACACGCTGAACCGCTACGGCTCCGAG
CAGGCGCTCGGGCTGCTCGTCGCGCTGTCGCTCGTGCGCGAGCTCGGCCCGGTCGTCACCGCGCTGCTGTTCGCGGGCCG
CGCGGGCACGTCGCTGACTGCCGAAATCGGGCTGATGAAGGCCGGCGAGCAGCTGACCGCGCTCGAAATGATGGCCGTCG
ATCCGATCAAGACGGTGATCGCGCCGCGCATGTGGGCCGGGATCATCACGATGCCGCTGCTCGCGGCGATCTTCAACGCG
GTCGGCGTGCTCGGCGGCTATTTCGTCGGCGTCGTGCTGATCGGCGTCGATCCGGGCGCGTTCTGGTCGCAGATGCAGGG
CGGCGTCGAAGTGTGGGCCGACGTCGGCAACGGCGTGCTGAAAAGCATCGTGTTCGGCTTCGCGGTGACCTTCATTGCAC
TGTTTCAAGGGTACGAGGCGAAGCCCACGCCCGAGGGCGTGTCGCGTGCGACGACCAAGACCGTCGTGTTCGCATCGCTT
GCCGTACTCGGTCTCGATTTCCTGCTGACCGCACTGATGTTCAGCTAA

Upstream 100 bases:

>100_bases
GCGTGCGTCAGTTCATCGACGGCGCGCCGGACGGCCCGTTCAAATTTCATTACACGAGCCCGCCGCTGGCGGCGGATTTC
GGGCTCGGCGGAGGACGCGC

Downstream 100 bases:

>100_bases
GCCTCAGCCAAGCCAAATTTTGGGATGACGATGAAAAAGACTGCTCTCGACTTCTGGGTCGGCCTGTTCGTGGTGGTGGG
GTTCGTTGCGCTGCTGTTCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKTVIAPRMWAGIITMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVEVWADVGNGVLKSIVFGFAVTFIALFQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS

Sequences:

>Translated_255_residues
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKTVIAPRMWAGIITMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVEVWADVGNGVLKSIVFGFAVTFIALFQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS
>Mature_255_residues
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKTVIAPRMWAGIITMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVEVWADVGNGVLKSIVFGFAVTFIALFQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=220, Percent_Identity=60.9090909090909, Blast_Score=268, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27234; Mature: 27234

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGL
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHH
FVGFVLGLQGYYTLNRYGSEQALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMK
HHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AGEQLTALEMMAVDPIKTVIAPRMWAGIITMPLLAAIFNAVGVLGGYFVGVVLIGVDPGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
FWSQMQGGVEVWADVGNGVLKSIVFGFAVTFIALFQGYEAKPTPEGVSRATTKTVVFASL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AVLGLDFLLTALMFS
HHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]