Click here to switch to the map view.

The map label for this gene is gltD [H]

Identifier: 161523486

GI number: 161523486

Start: 337794

End: 339260

Strand: Direct

Name: gltD [H]

Synonym: Bmul_0306

Alternate gene names: 161523486

Gene position: 337794-339260 (Clockwise)

Preceding gene: 161523485

Following gene: 161523487

Centisome position: 9.8

GC content: 66.94

Gene sequence:

>1467_bases
ATGGGCAAGGCAACCGGTTTTCTGGAGTTCGAACGCCGCCACGAGGCGTACGAAGCACCGCTCACGCGCGTGAAGCACTA
CAAGGAATTCGTCGCGGCGCTGTCCGACGCGGACGCGAAGATCCAGGGCGCGCGCTGCATGGACTGCGGCATCCCGTTCT
GCAACAACGGCTGCCCGGTCAACAACATCATTCCGGACTTCAACGATCTCGTTTACCGCCAGGACTGGCAGCAGGCGATC
GAAGTCCTGCATTCGACGAACAACTTCCCCGAGTTCACGGGCCGCATCTGCCCGGCGCCGTGCGAAGCGGCCTGCACGCT
CGGCATCAACGACGATCCGGTCGGCATCAAGTCGATCGAGCACGCGATCATCGACAAGGCGTGGGCCGAAGGCTGGGTGA
AGCCGCAGCCGGCCGCGCACAAGACGGGCAAGAAGGTCGCCGTCGTCGGCTCGGGCCCGGCGGGCCTCGCGGCTGCGCAG
CAGCTCGCGCGCGCGGGCCACGACGTGACGGTGTTCGAGAAGAACGACCGGATCGGCGGCCTGCTGCGTTACGGGATTCC
CGATTTCAAGCTCGAGAAGTGGCTGATCGATCGCCGGATGCGCCAGATGGAAGCGGAAGGCGTGACGTTCCGCACGAGCG
TGTTCATCGGCAAGGATCCGCTGCCCGAGTCGATCGGCAATCTCGCGAAGGAAACGATCTCGCCGGAGACGCTGAAGGAG
GCGTTCGACGCGGTCGTGATCGCGGGCGGCTCGGAGACGCCGCGCGATCTGCCGGTGCCGGGCCGCGAACTGGCCGGCAT
CCATTTCGCGATGGACTTCCTGCCGCAGCAGAACCGCGTGAACGCCGGCGACAAGCTCGTCGATCAGTTGCTCGCCAAGG
GCAAGCACGTCGTCGTGATCGGCGGCGGCGACACCGGTTCGGACTGCGTCGGCACGTCGAACCGCCACGGCGCGAAGCAC
GTCACGCAGTTCGAACTGCTGCCGCAGCCGCCGGAAGAGGAAAACAAGCCGCTCGTGTGGCCGTACTGGCCGATCAAGCT
GCGCACGTCGTCGTCGCACGAGGAAGGCTGCGAGCGCGACTGGGCAGTCGCGACGAAGCGCTTCGAAGGCAAGAACGGCA
AGGTCGAGAAGCTCGTCGCGGCGCGCGTCGAGTGGAAGGACGGCAAGATGCAGGAAGTGCCGGGCTCCGAGTTCGAGATG
AAGGCCGATCTCGTGCTGCTCGCGATGGGCTTCACGCAGCCGGCCGCGCCGGTGCTCGAGGCGTTCGGCGTCGCGAAGGA
TGCGCGCGGCAACGCGCGCGCGGCGACCGAAGGCGATCGCGCGTACTACACGTCGGTCGACAAGGTGTTCGCGGCCGGCG
ACATGCGCCGCGGCCAGTCGCTCGTCGTCTGGGCGATCCGCGAAGGCCGCCAGTGCGCGCGCTCGGTCGACGCGTACCTG
ATGGGGCATTCGGAACTGCCGCGCTAA

Upstream 100 bases:

>100_bases
GCGCTGGCCGCCTGAGCGACGAACGACATAGCGAAGGCCGCGCGCGCATGAACGCCGCGCGCGGCTCCCCCACCCGATAC
ATCGAACAGAAGAGCACCTT

Downstream 100 bases:

>100_bases
GCACCGAGAGCGGGTTTCGATGAAGCCGGGCGTCCGAGAGGGCGCCCGGTTTTTTTTCGGCCGCGCACCCGCGCGCACGC
GGTTTCTTGAGCGCGTTTCT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDADAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVKPQPAAHKTGKKVAVVGSGPAGLAAAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGNLAKETISPETLKE
AFDAVVIAGGSETPRDLPVPGRELAGIHFAMDFLPQQNRVNAGDKLVDQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKH
VTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLVAARVEWKDGKMQEVPGSEFEM
KADLVLLAMGFTQPAAPVLEAFGVAKDARGNARAATEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
MGHSELPR

Sequences:

>Translated_488_residues
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDADAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVKPQPAAHKTGKKVAVVGSGPAGLAAAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGNLAKETISPETLKE
AFDAVVIAGGSETPRDLPVPGRELAGIHFAMDFLPQQNRVNAGDKLVDQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKH
VTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLVAARVEWKDGKMQEVPGSEFEM
KADLVLLAMGFTQPAAPVLEAFGVAKDARGNARAATEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
MGHSELPR
>Mature_487_residues
GKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDADAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAIE
VLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVKPQPAAHKTGKKVAVVGSGPAGLAAAQQ
LARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGNLAKETISPETLKEA
FDAVVIAGGSETPRDLPVPGRELAGIHFAMDFLPQQNRVNAGDKLVDQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKHV
TQFELLPQPPEEENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLVAARVEWKDGKMQEVPGSEFEMK
ADLVLLAMGFTQPAAPVLEAFGVAKDARGNARAATEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYLM
GHSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=491, Percent_Identity=25.6619144602851, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1789606, Length=486, Percent_Identity=34.7736625514403, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788811, Length=467, Percent_Identity=35.5460385438972, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082180, Length=467, Percent_Identity=33.8329764453961, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1788468, Length=462, Percent_Identity=28.5714285714286, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789243, Length=438, Percent_Identity=28.310502283105, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=508, Percent_Identity=45.6692913385827, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71984108, Length=483, Percent_Identity=26.7080745341615, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320030, Length=502, Percent_Identity=49.601593625498, Blast_Score=473, Evalue=1e-134,
Organism=Drosophila melanogaster, GI28574881, Length=503, Percent_Identity=49.7017892644135, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665539, Length=503, Percent_Identity=49.7017892644135, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665547, Length=503, Percent_Identity=49.5029821073559, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665543, Length=503, Percent_Identity=49.5029821073559, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24640763, Length=487, Percent_Identity=25.2566735112936, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI18858217, Length=487, Percent_Identity=25.2566735112936, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53512; Mature: 53381

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDADAKIQGARCMDCGIPFCNNGCPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWQQAIEVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAEGWVKPQPAAHKTGKKVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGNLAKETISPETLKE
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHH
AFDAVVIAGGSETPRDLPVPGRELAGIHFAMDFLPQQNRVNAGDKLVDQLLAKGKHVVVI
HHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKHVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHEEGCERD
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCHHHCCHH
WAVATKRFEGKNGKVEKLVAARVEWKDGKMQEVPGSEFEMKADLVLLAMGFTQPAAPVLE
HHHHHHHCCCCCCCHHHHHHHHEECCCCCCCCCCCCCCEEECCEEEEEECCCCCHHHHHH
AFGVAKDARGNARAATEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHH
MGHSELPR
CCCCCCCC
>Mature Secondary Structure 
GKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDADAKIQGARCMDCGIPFCNNGCPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWQQAIEVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAEGWVKPQPAAHKTGKKVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGNLAKETISPETLKE
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHH
AFDAVVIAGGSETPRDLPVPGRELAGIHFAMDFLPQQNRVNAGDKLVDQLLAKGKHVVVI
HHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKHVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHEEGCERD
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCHHHCCHH
WAVATKRFEGKNGKVEKLVAARVEWKDGKMQEVPGSEFEMKADLVLLAMGFTQPAAPVLE
HHHHHHHCCCCCCCHHHHHHHHEECCCCCCCCCCCCCCEEECCEEEEEECCCCCHHHHHH
AFGVAKDARGNARAATEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHH
MGHSELPR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]