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The map label for this gene is cof [C]

Identifier: 161523336

GI number: 161523336

Start: 177131

End: 177952

Strand: Direct

Name: cof [C]

Synonym: Bmul_0156

Alternate gene names: 161523336

Gene position: 177131-177952 (Clockwise)

Preceding gene: 161523335

Following gene: 161523338

Centisome position: 5.14

GC content: 66.3

Gene sequence:

>822_bases
ATGTACAAAGTCATCGCCACCGATCTCGACGGCACCCTGCTGAACAGCGACCACCAGCTCGATCCGTACACGATCGAGAC
CGTCCGTCGGCTCGACCGCGACGGCGTGCAGTTCGTGATCGCAACGGGGCGCCACTACGCGGACGTCGCCGGCATCCGCG
ACGTGCTCGGCATCCGCCCGTACCTGATCACGTCGAACGGCGCGCGCGTCCATGCGCCGGACGATACGACGATCCACGCG
CAGGACATCGATCCGGCGATCGTGCGCCGTCTGGTGCTGCCCGAGGTCGCCGGCGCGCACGGCCGCGTGATCGTCAATCT
GTTTACCGAACACGGCTGGCTGATCGACCGCGATGCGCCGCACCTGCTCGAATTCCATCAGGACTCGGGCTTCCGGTACG
AAGTGATCGACATGTTCGCGCACGACGGCGCGGACATCGCGAAGGTGCTGTATATCGGCGAACCGGCCGATCTGGCGGTC
GTCGCCGAGCAGATGCGCGTGCAGTTCGGCGATGCGCTGTACGTCACGTACTCGCTGCCCGACTGTCTCGAGGTGATGAC
CGCGAACGTGTCGAAGGGCCGCGCGCTGCGTGCGGTGCTCGGGCGGCTCGGCGTCGACGCCGGCCATTGCGTCGCGTTCG
GCGACAACATGAACGACATCGACCTGCTCGAAACGGCCGGCCATGCGTTCATGATGAACAACGCAAACCCGGATCTGATC
GCGCGGCTGCCGCACATCCCGCGGATCGGCAACAACTTCGACGCGGGCGTCGCGCGCCATCTGCGCACGCTGTTCGCGCT
CGAGGATCTCGCCGCGTCCTGA

Upstream 100 bases:

>100_bases
GAGCTGGAGCTGGAGCTGGAATCCCGCCGCCCCGACACCCACCCCCACCCGACGTTAAAATGACGGCTCCGCAGCGCGCT
GCGCGCCTTCCTTGCTCCCC

Downstream 100 bases:

>100_bases
GTCCCGACATGAAAAACGGGCGCCAGCGGCGCCCGTCAAATGGCTTGCCTCTATCTTCTTGTGCAGCGTGAGGCTCGCTG
CTCTGCTCGCATGACCCCGT

Product: Cof-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MYKVIATDLDGTLLNSDHQLDPYTIETVRRLDRDGVQFVIATGRHYADVAGIRDVLGIRPYLITSNGARVHAPDDTTIHA
QDIDPAIVRRLVLPEVAGAHGRVIVNLFTEHGWLIDRDAPHLLEFHQDSGFRYEVIDMFAHDGADIAKVLYIGEPADLAV
VAEQMRVQFGDALYVTYSLPDCLEVMTANVSKGRALRAVLGRLGVDAGHCVAFGDNMNDIDLLETAGHAFMMNNANPDLI
ARLPHIPRIGNNFDAGVARHLRTLFALEDLAAS

Sequences:

>Translated_273_residues
MYKVIATDLDGTLLNSDHQLDPYTIETVRRLDRDGVQFVIATGRHYADVAGIRDVLGIRPYLITSNGARVHAPDDTTIHA
QDIDPAIVRRLVLPEVAGAHGRVIVNLFTEHGWLIDRDAPHLLEFHQDSGFRYEVIDMFAHDGADIAKVLYIGEPADLAV
VAEQMRVQFGDALYVTYSLPDCLEVMTANVSKGRALRAVLGRLGVDAGHCVAFGDNMNDIDLLETAGHAFMMNNANPDLI
ARLPHIPRIGNNFDAGVARHLRTLFALEDLAAS
>Mature_273_residues
MYKVIATDLDGTLLNSDHQLDPYTIETVRRLDRDGVQFVIATGRHYADVAGIRDVLGIRPYLITSNGARVHAPDDTTIHA
QDIDPAIVRRLVLPEVAGAHGRVIVNLFTEHGWLIDRDAPHLLEFHQDSGFRYEVIDMFAHDGADIAKVLYIGEPADLAV
VAEQMRVQFGDALYVTYSLPDCLEVMTANVSKGRALRAVLGRLGVDAGHCVAFGDNMNDIDLLETAGHAFMMNNANPDLI
ARLPHIPRIGNNFDAGVARHLRTLFALEDLAAS

Specific function: Unknown

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI48994981, Length=266, Percent_Identity=39.4736842105263, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI87081741, Length=261, Percent_Identity=36.7816091954023, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI87081790, Length=264, Percent_Identity=22.7272727272727, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 30011; Mature: 30011

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKVIATDLDGTLLNSDHQLDPYTIETVRRLDRDGVQFVIATGRHYADVAGIRDVLGIRP
CEEEEEECCCCEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCC
YLITSNGARVHAPDDTTIHAQDIDPAIVRRLVLPEVAGAHGRVIVNLFTEHGWLIDRDAP
EEEECCCCEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCH
HLLEFHQDSGFRYEVIDMFAHDGADIAKVLYIGEPADLAVVAEQMRVQFGDALYVTYSLP
HHHHHHCCCCCEEEEEEEHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCH
DCLEVMTANVSKGRALRAVLGRLGVDAGHCVAFGDNMNDIDLLETAGHAFMMNNANPDLI
HHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHCCCEEEECCCCHHHE
ARLPHIPRIGNNFDAGVARHLRTLFALEDLAAS
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYKVIATDLDGTLLNSDHQLDPYTIETVRRLDRDGVQFVIATGRHYADVAGIRDVLGIRP
CEEEEEECCCCEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCC
YLITSNGARVHAPDDTTIHAQDIDPAIVRRLVLPEVAGAHGRVIVNLFTEHGWLIDRDAP
EEEECCCCEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCH
HLLEFHQDSGFRYEVIDMFAHDGADIAKVLYIGEPADLAVVAEQMRVQFGDALYVTYSLP
HHHHHHCCCCCEEEEEEEHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCH
DCLEVMTANVSKGRALRAVLGRLGVDAGHCVAFGDNMNDIDLLETAGHAFMMNNANPDLI
HHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHCCCEEEECCCCHHHE
ARLPHIPRIGNNFDAGVARHLRTLFALEDLAAS
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]