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The map label for this gene is mnmC

Identifier: 161523247

GI number: 161523247

Start: 70818

End: 72791

Strand: Direct

Name: mnmC

Synonym: Bmul_0067

Alternate gene names: 161523247

Gene position: 70818-72791 (Clockwise)

Preceding gene: 161523245

Following gene: 161523248

Centisome position: 2.05

GC content: 70.92

Gene sequence:

>1974_bases
ATGCCCGACCGACTCGTCCCTGCCACGCTCGCGTTCCGCGACGACGGCACCCTCGTCTCGCCCGCGTACGGCGACATCTA
TCACAGTGCCGCGGGCGCGATCGCGCAGGCCAACCACGTGTTCGTCGCGGGCAACGGGCTGCCCGCGCGCTGGCAGCAGC
GCCGCACGTTCACGATCGTCGAGACGGGCTTCGGCACCGGCTGCAACTTTCTCGCCACGTGGGCCGCGTGGCGCGACGAT
CCCGCGCGCTGCGAGCGTCTCCATTTCGTGTCGGTCGAAAAGCATCCGTTCTCGCGTGAAGACCTGCGTCGCGCCGCTGC
GCATATCGTTGCGAACACAACCATATCGGCGAGCGTCGATGCGCTCGCCGATGCATGGCCGCCGCTCGTGCCGGGCCTGC
ATCGACTCGAATTCGATGCAGGGCGCGTCGTGCTCACGCTCGTATTCGGCGATGCGCTCGAGCGGCTGCCGACACTCGTC
GCACGCGCCGATGCGTTTTATCTCGACGGTTTTGCGCCGTCGAAGAACGCGGACCTCTGGTCGATCGACGTGTTTCGCGC
GCTTGCGCGCATGGCCGACGAGCACGCGACGTTCGCGACCTATTCGAGTTCCGGCGTCGTGAAACGCGCGCTCGACGAAG
CGGGTTTCGCGTATCGCAAGGTCGACGGTTTCGCCGGCAAGCGCGCGATGCTGGTCGGCGAATATGCGCCGCGCTGGCGC
ATGCGCCGGCACGAACCGCCGCGCGCATGGCCGAACGCCGCCGCACGCCGTGCGCTCGTGATCGGTGCCGGCGTTGCAGG
CTGTGCGGTCGTCGAGCGGCTCGCCGCGCGCGGCTGGAACGTAACGCTGATCGAACGGCACGAACGGATCGCGAGCGAAG
CGTCCGGCAATCCGGCCGGTGTATTCCATCCGCTGATGACGCGCGACGACAACGTCGCGAGCCGTCTCACGCGCGCCGGT
TTTCTCTATGCGCTCGCGCGCTGGCGGGCGCTCGAAGCGGGCGGTCATGCGTTTGCGCGCAGCACGCACGGCATGGTTCA
TCTCGCCGAATCGGCGGACGACTTCGCGCGCATGCGCGACGCATTCGATGCGCTCGGCGCGCCGTCCGACTACGCGCGGC
TGCTCGACACGGACGCCGCGCGCGCCTATCTGAATCTGCCGGTCGCGCATGGCGGGCTGCTGTTTCCGCACGGCGGTGCG
GTGTGGCCCGCCGCACTCGCCGACGCGCAATGCGCGGCCGCCGGCGATCGCGTGCAACGGCTGACGCGCACCGAAGTCGC
GCGGCTCGAACGCAACGGCGACGAGTGGCATGCGCTCGATGCGCACGGACGCACGCTCGCGCAGGCGCCCGTCGTCGTAC
TCGCGAATGCCGGCGATGCGGTGCGGCTTGCCGGGCTGCGGCACGTCGCGCTGCAACCGGTGCGCGGGCAGCTCACGCTG
CTGCCGGCAGCCAGCGCATCGCCGCCGCCGTGCCCGGCGATCGGCGACGGCTACGCGGTGCCGCTCGACGACGGCACGCT
GCTGATCGGCGCGACCTTCGAACCGGACGACGTCGATCCGGCGATACGCGTGGCCGGCCATGCGGAAAACCTCGAGCGTG
TGCGGCGCCTGCTGCCGGGACTGATCGGCGACGTGCCCGCGCTCGACACGCTGCGCGGCCGCGTCGCGTTCCGCTGGGTC
GCCGGCGACCGGCTGCCGCTGATCGGCCCGCTCGCCGACGAAGCGCAGGCTGTGGCAAACGCCCGCGCGCTGAGCGGCGC
CAAAGCCCGCGACCTGCCGCGCATGCCGGGGCTCTACGGCGCATTCGGCTACGGCTCGCGCGGCCTCGTCTGGGCCGCGC
TCGGTGCCGAGCTCATCGCCTCGCAACTCGACGGCGAGCCCTGGCCGATCGAGCGCGAACTCGCAGAAGCCGTCGATCCC
GCCCGTTTCCTGATCCGCGCATTGCGCGCACGCCAGGTGAGCGCCGCCGACTGA

Upstream 100 bases:

>100_bases
CGCTGCGCGCCTGCGTCGCGCCGTTGGATTTTGCCAACGGCCGATCGGCACGTGCGCCGCAATCCCTTGCCAGGCTTGGC
TGTCACGATTTTTGTTTCGC

Downstream 100 bases:

>100_bases
TCCGGCGCACCGTTCCCTCACAGTTTCCCGATTTTCCCGCGCAAAGTTATCCACGCGACGCTGTGGATAACCGCGCGAAA
TCCATGCTCGGTTCGTGTGC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MPDRLVPATLAFRDDGTLVSPAYGDIYHSAAGAIAQANHVFVAGNGLPARWQQRRTFTIVETGFGTGCNFLATWAAWRDD
PARCERLHFVSVEKHPFSREDLRRAAAHIVANTTISASVDALADAWPPLVPGLHRLEFDAGRVVLTLVFGDALERLPTLV
ARADAFYLDGFAPSKNADLWSIDVFRALARMADEHATFATYSSSGVVKRALDEAGFAYRKVDGFAGKRAMLVGEYAPRWR
MRRHEPPRAWPNAAARRALVIGAGVAGCAVVERLAARGWNVTLIERHERIASEASGNPAGVFHPLMTRDDNVASRLTRAG
FLYALARWRALEAGGHAFARSTHGMVHLAESADDFARMRDAFDALGAPSDYARLLDTDAARAYLNLPVAHGGLLFPHGGA
VWPAALADAQCAAAGDRVQRLTRTEVARLERNGDEWHALDAHGRTLAQAPVVVLANAGDAVRLAGLRHVALQPVRGQLTL
LPAASASPPPCPAIGDGYAVPLDDGTLLIGATFEPDDVDPAIRVAGHAENLERVRRLLPGLIGDVPALDTLRGRVAFRWV
AGDRLPLIGPLADEAQAVANARALSGAKARDLPRMPGLYGAFGYGSRGLVWAALGAELIASQLDGEPWPIERELAEAVDP
ARFLIRALRARQVSAAD

Sequences:

>Translated_657_residues
MPDRLVPATLAFRDDGTLVSPAYGDIYHSAAGAIAQANHVFVAGNGLPARWQQRRTFTIVETGFGTGCNFLATWAAWRDD
PARCERLHFVSVEKHPFSREDLRRAAAHIVANTTISASVDALADAWPPLVPGLHRLEFDAGRVVLTLVFGDALERLPTLV
ARADAFYLDGFAPSKNADLWSIDVFRALARMADEHATFATYSSSGVVKRALDEAGFAYRKVDGFAGKRAMLVGEYAPRWR
MRRHEPPRAWPNAAARRALVIGAGVAGCAVVERLAARGWNVTLIERHERIASEASGNPAGVFHPLMTRDDNVASRLTRAG
FLYALARWRALEAGGHAFARSTHGMVHLAESADDFARMRDAFDALGAPSDYARLLDTDAARAYLNLPVAHGGLLFPHGGA
VWPAALADAQCAAAGDRVQRLTRTEVARLERNGDEWHALDAHGRTLAQAPVVVLANAGDAVRLAGLRHVALQPVRGQLTL
LPAASASPPPCPAIGDGYAVPLDDGTLLIGATFEPDDVDPAIRVAGHAENLERVRRLLPGLIGDVPALDTLRGRVAFRWV
AGDRLPLIGPLADEAQAVANARALSGAKARDLPRMPGLYGAFGYGSRGLVWAALGAELIASQLDGEPWPIERELAEAVDP
ARFLIRALRARQVSAAD
>Mature_656_residues
PDRLVPATLAFRDDGTLVSPAYGDIYHSAAGAIAQANHVFVAGNGLPARWQQRRTFTIVETGFGTGCNFLATWAAWRDDP
ARCERLHFVSVEKHPFSREDLRRAAAHIVANTTISASVDALADAWPPLVPGLHRLEFDAGRVVLTLVFGDALERLPTLVA
RADAFYLDGFAPSKNADLWSIDVFRALARMADEHATFATYSSSGVVKRALDEAGFAYRKVDGFAGKRAMLVGEYAPRWRM
RRHEPPRAWPNAAARRALVIGAGVAGCAVVERLAARGWNVTLIERHERIASEASGNPAGVFHPLMTRDDNVASRLTRAGF
LYALARWRALEAGGHAFARSTHGMVHLAESADDFARMRDAFDALGAPSDYARLLDTDAARAYLNLPVAHGGLLFPHGGAV
WPAALADAQCAAAGDRVQRLTRTEVARLERNGDEWHALDAHGRTLAQAPVVVLANAGDAVRLAGLRHVALQPVRGQLTLL
PAASASPPPCPAIGDGYAVPLDDGTLLIGATFEPDDVDPAIRVAGHAENLERVRRLLPGLIGDVPALDTLRGRVAFRWVA
GDRLPLIGPLADEAQAVANARALSGAKARDLPRMPGLYGAFGYGSRGLVWAALGAELIASQLDGEPWPIERELAEAVDPA
RFLIRALRARQVSAAD

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=666, Percent_Identity=31.0810810810811, Blast_Score=275, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_BURM1 (A9AJC5)

Other databases:

- EMBL:   CP000868
- EMBL:   AP009385
- RefSeq:   YP_001578259.1
- RefSeq:   YP_001944524.1
- ProteinModelPortal:   A9AJC5
- SMR:   A9AJC5
- GeneID:   5765086
- GeneID:   6357190
- GenomeReviews:   AP009385_GR
- GenomeReviews:   CP000868_GR
- KEGG:   bmj:BMULJ_00003
- KEGG:   bmu:Bmul_0067
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 70582; Mature: 70451

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00646 RIBOSOMAL_S13_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRLVPATLAFRDDGTLVSPAYGDIYHSAAGAIAQANHVFVAGNGLPARWQQRRTFTIV
CCCCCCCEEEEECCCCCEECCCHHHHHHHHHHHHEECCEEEEECCCCCCHHHCCCEEEEE
ETGFGTGCNFLATWAAWRDDPARCERLHFVSVEKHPFSREDLRRAAAHIVANTTISASVD
ECCCCCCCHHHHHHHHHCCCHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHH
ALADAWPPLVPGLHRLEFDAGRVVLTLVFGDALERLPTLVARADAFYLDGFAPSKNADLW
HHHHCCCCCCCCCHHEECCCCCEEEEEHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEE
SIDVFRALARMADEHATFATYSSSGVVKRALDEAGFAYRKVDGFAGKRAMLVGEYAPRWR
EHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCHHH
MRRHEPPRAWPNAAARRALVIGAGVAGCAVVERLAARGWNVTLIERHERIASEASGNPAG
HHCCCCCCCCCCHHHCEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCE
VFHPLMTRDDNVASRLTRAGFLYALARWRALEAGGHAFARSTHGMVHLAESADDFARMRD
EECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCHHHHHHHHH
AFDALGAPSDYARLLDTDAARAYLNLPVAHGGLLFPHGGAVWPAALADAQCAAAGDRVQR
HHHHCCCCHHHHHHHCCHHHHHEEECEEECCCEEECCCCCCCHHHHCCCHHHHHHHHHHH
LTRTEVARLERNGDEWHALDAHGRTLAQAPVVVLANAGDAVRLAGLRHVALQPVRGQLTL
HHHHHHHHHHCCCCCEEEECCCCCCHHCCCEEEEECCCCEEEHHHHHHHHHCCCCCEEEE
LPAASASPPPCPAIGDGYAVPLDDGTLLIGATFEPDDVDPAIRVAGHAENLERVRRLLPG
EECCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHEECCCHHHHHHHHHHHHH
LIGDVPALDTLRGRVAFRWVAGDRLPLIGPLADEAQAVANARALSGAKARDLPRMPGLYG
HHCCCCCHHHHCCCEEEEEECCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
AFGYGSRGLVWAALGAELIASQLDGEPWPIERELAEAVDPARFLIRALRARQVSAAD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PDRLVPATLAFRDDGTLVSPAYGDIYHSAAGAIAQANHVFVAGNGLPARWQQRRTFTIV
CCCCCCEEEEECCCCCEECCCHHHHHHHHHHHHEECCEEEEECCCCCCHHHCCCEEEEE
ETGFGTGCNFLATWAAWRDDPARCERLHFVSVEKHPFSREDLRRAAAHIVANTTISASVD
ECCCCCCCHHHHHHHHHCCCHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHH
ALADAWPPLVPGLHRLEFDAGRVVLTLVFGDALERLPTLVARADAFYLDGFAPSKNADLW
HHHHCCCCCCCCCHHEECCCCCEEEEEHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEE
SIDVFRALARMADEHATFATYSSSGVVKRALDEAGFAYRKVDGFAGKRAMLVGEYAPRWR
EHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCHHH
MRRHEPPRAWPNAAARRALVIGAGVAGCAVVERLAARGWNVTLIERHERIASEASGNPAG
HHCCCCCCCCCCHHHCEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCE
VFHPLMTRDDNVASRLTRAGFLYALARWRALEAGGHAFARSTHGMVHLAESADDFARMRD
EECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCHHHHHHHHH
AFDALGAPSDYARLLDTDAARAYLNLPVAHGGLLFPHGGAVWPAALADAQCAAAGDRVQR
HHHHCCCCHHHHHHHCCHHHHHEEECEEECCCEEECCCCCCCHHHHCCCHHHHHHHHHHH
LTRTEVARLERNGDEWHALDAHGRTLAQAPVVVLANAGDAVRLAGLRHVALQPVRGQLTL
HHHHHHHHHHCCCCCEEEECCCCCCHHCCCEEEEECCCCEEEHHHHHHHHHCCCCCEEEE
LPAASASPPPCPAIGDGYAVPLDDGTLLIGATFEPDDVDPAIRVAGHAENLERVRRLLPG
EECCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHEECCCHHHHHHHHHHHHH
LIGDVPALDTLRGRVAFRWVAGDRLPLIGPLADEAQAVANARALSGAKARDLPRMPGLYG
HHCCCCCHHHHCCCEEEEEECCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
AFGYGSRGLVWAALGAELIASQLDGEPWPIERELAEAVDPARFLIRALRARQVSAAD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA