Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 161523228

GI number: 161523228

Start: 51492

End: 53054

Strand: Reverse

Name: emrB [H]

Synonym: Bmul_0048

Alternate gene names: 161523228

Gene position: 53054-51492 (Counterclockwise)

Preceding gene: 161523229

Following gene: 161523226

Centisome position: 1.54

GC content: 66.86

Gene sequence:

>1563_bases
ATGTCCGCCACCACGGCATCCGCCGCGTCCCCTGCCGCCGAACCGGCGCCGCTGTCCGGCGGCGCCCTCGCGCTGCTCAC
CGTCGGGCTCGCGCTCGGCACGTTCATGGAAGTGCTCGACACGTCGATCGCGAACGTCGCGGTGCCGACGATTTCGGGCA
GCCTCGGCGTCGCGACCAGCGAAGGCACGTGGGTGATTTCGTCGTATTCGGTCGCGTCCGCGATCGCGGTACCGCTGACC
GGCTGGCTCGCGCGGCGCGTCGGCGAAGTGCGGCTGTTCACGCTGTCGGTGCTCGCGTTCACGATCGCGTCGGCGCTCTG
CGGCCTTGCGTCGAACTTCGAGACGCTGATCGCGTTCCGGCTGCTGCAGGGCCTCGTGTCGGGGCCGATGGTGCCGCTGT
CGCAGACGATCCTGATGCGCAGCTACCCGCCCGCGAAGCGCGGGCTCGCGCTCGGCCTATGGGCGATGACGGTGATCGTC
GCGCCGATCTTCGGCCCGCTGCTCGGCGGCTGGATCAGCGACAACTACACGTGGCCGTGGATCTTCTACATCAACCTGCC
GATCGGGATTTTCTCCGCGACCTGCGCGTTCTTCCTGCTGCGCGGCCGCGAGACGAAGACGACGAAACAGCGGATCGACG
CAGTCGGCCTCGCGCTGCTCGTGATCGGCGTGTCGTGTCTGCAGATGATGCTCGACCTCGGCAAGGACCGCGACTGGTTC
AACTCGTCGTTCATCGTCGCGCTCGCGCTGATCGCGGTCGTGTCGCTCGCGTTCATGCTCGTCTGGGAAGCGACCGAGAA
GGAGCCGGTGGTCGATCTCACGCTGTTCAAGGATCGCAACTTCGCGCTCGGCGCGCTGATCATCTCGTTCGGCTTCATGG
CGTTCTTCGGCTCGGTCGTGGTCTTCCCGCTGTGGCTGCAGACCGTGATGGGCTACACGGCCGGCAAGGCCGGCCTCGCG
ACCGCGCCGGTCGGGCTGCTCGCGCTCGTGCTGTCACCGCTGATCGGCCGCAACATGCACCGGCTCGACCTGCGGATGGT
CGCGAGCTTCGCCTTCATCGTGTTCGCGATCGTGTCGGTATGGAACTCGACGTTTACGCTCGACGTGCCGTTCAACCACG
TGATCCTGCCGCGGCTCGTGCAGGGAATCGGCGTCGCGTGCTTCTTCGTGCCGATGACGACGATCACGCTGTCGAGCATC
CCCGACGAACGGCTCGCGAGCGCCTCGGGCCTGTCCAACTTCCTGCGTACGCTGTCGGGCGCGATCGGCACCGCGGTCAG
CTCGACGTTCTGGGAGAACGACGCGATCTACCACCACGCGCGGCTCGCCGAATCGGTGAGCGTCTATGCGCAGAACACGA
CCGACTATCAGGGCGCGCTGGCGCAGCTCGGCGTCGTCGGCCAGACCGCGAACGCGCAACTGAACCAGATCGTCACGCAG
CAGGGCTTCATGATGGCGACCAACGACTTCTTCCACCTGTCGGCGATCGTGTTCGTCGCGCTCGCGGCGCTCGTGTGGAT
CACAAAGCCGAAGAAGGGCGCGGGGCCGGCGATGGGGCATTGA

Upstream 100 bases:

>100_bases
GCAGAAGATGACGCGACATATGCCCGTCTTCCGCTGCCTGCCGTACCGCCCCGCTACGCGTTGCTGCGCTGCGGGCGCGA
GCCGATCCTAGGAGATTCCG

Downstream 100 bases:

>100_bases
CGGTCCATCGCCCGCGGGACGTGTCCTGTGTCGTCTGGCCGTTGGCGTCGCTTCGGCCAAAGTCGGTTCGAATCCAAATC
GAACCGCGTCACTCCCGCGC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSATTASAASPAAEPAPLSGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLT
GWLARRVGEVRLFTLSVLAFTIASALCGLASNFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIV
APIFGPLLGGWISDNYTWPWIFYINLPIGIFSATCAFFLLRGRETKTTKQRIDAVGLALLVIGVSCLQMMLDLGKDRDWF
NSSFIVALALIAVVSLAFMLVWEATEKEPVVDLTLFKDRNFALGALIISFGFMAFFGSVVVFPLWLQTVMGYTAGKAGLA
TAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAIVSVWNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSI
PDERLASASGLSNFLRTLSGAIGTAVSSTFWENDAIYHHARLAESVSVYAQNTTDYQGALAQLGVVGQTANAQLNQIVTQ
QGFMMATNDFFHLSAIVFVALAALVWITKPKKGAGPAMGH

Sequences:

>Translated_520_residues
MSATTASAASPAAEPAPLSGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLT
GWLARRVGEVRLFTLSVLAFTIASALCGLASNFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIV
APIFGPLLGGWISDNYTWPWIFYINLPIGIFSATCAFFLLRGRETKTTKQRIDAVGLALLVIGVSCLQMMLDLGKDRDWF
NSSFIVALALIAVVSLAFMLVWEATEKEPVVDLTLFKDRNFALGALIISFGFMAFFGSVVVFPLWLQTVMGYTAGKAGLA
TAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAIVSVWNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSI
PDERLASASGLSNFLRTLSGAIGTAVSSTFWENDAIYHHARLAESVSVYAQNTTDYQGALAQLGVVGQTANAQLNQIVTQ
QGFMMATNDFFHLSAIVFVALAALVWITKPKKGAGPAMGH
>Mature_519_residues
SATTASAASPAAEPAPLSGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLTG
WLARRVGEVRLFTLSVLAFTIASALCGLASNFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVA
PIFGPLLGGWISDNYTWPWIFYINLPIGIFSATCAFFLLRGRETKTTKQRIDAVGLALLVIGVSCLQMMLDLGKDRDWFN
SSFIVALALIAVVSLAFMLVWEATEKEPVVDLTLFKDRNFALGALIISFGFMAFFGSVVVFPLWLQTVMGYTAGKAGLAT
APVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAIVSVWNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSIP
DERLASASGLSNFLRTLSGAIGTAVSSTFWENDAIYHHARLAESVSVYAQNTTDYQGALAQLGVVGQTANAQLNQIVTQQ
GFMMATNDFFHLSAIVFVALAALVWITKPKKGAGPAMGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=498, Percent_Identity=53.4136546184739, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1788710, Length=514, Percent_Identity=47.6653696498054, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1790195, Length=411, Percent_Identity=30.9002433090024, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788392, Length=401, Percent_Identity=29.426433915212, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=25.4761904761905, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322958, Length=215, Percent_Identity=30.2325581395349, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=30.2325581395349, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=306, Percent_Identity=24.8366013071895, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55317; Mature: 55185

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATTASAASPAAEPAPLSGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEC
EGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLAFTIASALCGLASNFETLIAFR
CCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVAPIFGPLLGGWISDNYTWPW
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEE
IFYINLPIGIFSATCAFFLLRGRETKTTKQRIDAVGLALLVIGVSCLQMMLDLGKDRDWF
EEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NSSFIVALALIAVVSLAFMLVWEATEKEPVVDLTLFKDRNFALGALIISFGFMAFFGSVV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
VFPLWLQTVMGYTAGKAGLATAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAIVSV
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
WNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSIPDERLASASGLSNFLRTLSG
HCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AIGTAVSSTFWENDAIYHHARLAESVSVYAQNTTDYQGALAQLGVVGQTANAQLNQIVTQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
QGFMMATNDFFHLSAIVFVALAALVWITKPKKGAGPAMGH
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SATTASAASPAAEPAPLSGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEC
EGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLAFTIASALCGLASNFETLIAFR
CCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVAPIFGPLLGGWISDNYTWPW
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEE
IFYINLPIGIFSATCAFFLLRGRETKTTKQRIDAVGLALLVIGVSCLQMMLDLGKDRDWF
EEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NSSFIVALALIAVVSLAFMLVWEATEKEPVVDLTLFKDRNFALGALIISFGFMAFFGSVV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
VFPLWLQTVMGYTAGKAGLATAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAIVSV
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
WNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSIPDERLASASGLSNFLRTLSG
HCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AIGTAVSSTFWENDAIYHHARLAESVSVYAQNTTDYQGALAQLGVVGQTANAQLNQIVTQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
QGFMMATNDFFHLSAIVFVALAALVWITKPKKGAGPAMGH
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]