Click here to switch to the map view.

The map label for this gene is tuf [H]

Identifier: 161511078

GI number: 161511078

Start: 495033

End: 496217

Strand: Direct

Name: tuf [H]

Synonym: BB0476

Alternate gene names: 161511078

Gene position: 495033-496217 (Clockwise)

Preceding gene: 15594819

Following gene: 15594822

Centisome position: 54.36

GC content: 35.95

Gene sequence:

>1185_bases
ATGGCAAAAGAAGTTTTTCAAAGAACAAAGCCGCACATGAATGTTGGAACAATAGGTCATGTTGATCATGGTAAAACAAC
ACTAACAGCGGCTATTAGTATTTATTGTTCAAAATTAAATAAAGATGCAAAAGCATTAAAGTATGAAGATATTGATAATG
CACCTGAAGAGAAAGCAAGAGGAATAACAATTAATGCTAGGCATATTGAGTACGAAACAGCTAATAGACATTATGCTCAT
GTAGATTGTCCAGGCCATGCCGATTATATAAAAAATATGATTACAGGAGCAGCTCAAATGGATGCAGCGATACTTTTAGT
TGCTGCTGATAGTGGTGCTGAGCCTCAAACAAAAGAGCATTTGCTTCTTGCTCAAAGAATGGGAATAAAGAAAATAATAG
TTTTTTTAAATAAATTGGACTTAGCAGATCCTGAACTTGTTGAGCTTGTTGAAGTTGAAGTTTTAGAACTTGTTGAAAAA
TATGGCTTTTCAGCTGATACTCCAATAATCAAAGGTTCAGCTTTTGGGGCTATGTCAAATCCAGAAGATCCTGAATCTAC
AAAATGCGTTAAAGAACTTCTTGAATCTATGGATAATTATTTTGATCTTCCAGAAAGAGATATTGACAAGCCATTTTTGC
TTGCTGTTGAAGATGTATTTTCTATTTCAGGAAGAGGCACTGTTGCTACTGGGCGTATTGAAAGAGGTATTATTAAAGTT
GGTCAAGAAGTTGAAATAGTTGGAATTAAAGAAACCAGAAAAACTACTGTTACTGGTGTTGAAATGTTCCAGAAAATTCT
TGAGCAAGGTCAAGCAGGGGATAATGTTGGTCTTCTTTTGAGAGGCGTTGATAAAAAAGACATTGAGAGGGGGCAAGTTT
TGTCAGCTCCAGGTACAATTACTCCACACAAGAAATTTAAAGCTTCAATTTATTGTTTGACTAAAGAAGAAGGCGGTAGG
CACAAGCCATTTTTCCCAGGGTATAGACCACAGTTCTTTTTTAGAACAACCGATGTTACTGGAGTTGTTGCTTTAGAGGG
CAAAGAAATGGTTATGCCTGGTGATAATGTTGATATTATTGTTGAGCTGATCTCTTCAATAGCTATGGATAAGAATGTAG
AATTTGCTGTTCGAGAAGGTGGAAGAACCGTTGCTTCAGGAAGAATTCTTGAGATATTGGAATAG

Upstream 100 bases:

>100_bases
TAGCTATTTTCTTATTTTAATAAGAATAAATTTGTTAAAAATTTTTTAAAGAGATTAAATGTAAGTAGCTATTTAGAATA
TGAAATTTAGGAGGTTAGTC

Downstream 100 bases:

>100_bases
GTTAAGACTTTAAGGATATATTAATACTCTCTAAAGTTTAGGAGAATAAATTGATTGCTAAAGATAAGATACGCGTAAGA
TTGTTTAGTTTTGATGTTAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEVFQRTKPHMNVGTIGHVDHGKTTLTAAISIYCSKLNKDAKALKYEDIDNAPEEKARGITINARHIEYETANRHYAH
VDCPGHADYIKNMITGAAQMDAAILLVAADSGAEPQTKEHLLLAQRMGIKKIIVFLNKLDLADPELVELVEVEVLELVEK
YGFSADTPIIKGSAFGAMSNPEDPESTKCVKELLESMDNYFDLPERDIDKPFLLAVEDVFSISGRGTVATGRIERGIIKV
GQEVEIVGIKETRKTTVTGVEMFQKILEQGQAGDNVGLLLRGVDKKDIERGQVLSAPGTITPHKKFKASIYCLTKEEGGR
HKPFFPGYRPQFFFRTTDVTGVVALEGKEMVMPGDNVDIIVELISSIAMDKNVEFAVREGGRTVASGRILEILE

Sequences:

>Translated_394_residues
MAKEVFQRTKPHMNVGTIGHVDHGKTTLTAAISIYCSKLNKDAKALKYEDIDNAPEEKARGITINARHIEYETANRHYAH
VDCPGHADYIKNMITGAAQMDAAILLVAADSGAEPQTKEHLLLAQRMGIKKIIVFLNKLDLADPELVELVEVEVLELVEK
YGFSADTPIIKGSAFGAMSNPEDPESTKCVKELLESMDNYFDLPERDIDKPFLLAVEDVFSISGRGTVATGRIERGIIKV
GQEVEIVGIKETRKTTVTGVEMFQKILEQGQAGDNVGLLLRGVDKKDIERGQVLSAPGTITPHKKFKASIYCLTKEEGGR
HKPFFPGYRPQFFFRTTDVTGVVALEGKEMVMPGDNVDIIVELISSIAMDKNVEFAVREGGRTVASGRILEILE
>Mature_393_residues
AKEVFQRTKPHMNVGTIGHVDHGKTTLTAAISIYCSKLNKDAKALKYEDIDNAPEEKARGITINARHIEYETANRHYAHV
DCPGHADYIKNMITGAAQMDAAILLVAADSGAEPQTKEHLLLAQRMGIKKIIVFLNKLDLADPELVELVEVEVLELVEKY
GFSADTPIIKGSAFGAMSNPEDPESTKCVKELLESMDNYFDLPERDIDKPFLLAVEDVFSISGRGTVATGRIERGIIKVG
QEVEIVGIKETRKTTVTGVEMFQKILEQGQAGDNVGLLLRGVDKKDIERGQVLSAPGTITPHKKFKASIYCLTKEEGGRH
KPFFPGYRPQFFFRTTDVTGVVALEGKEMVMPGDNVDIIVELISSIAMDKNVEFAVREGGRTVASGRILEILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=396, Percent_Identity=52.020202020202, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI4503475, Length=326, Percent_Identity=35.8895705521472, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4503471, Length=326, Percent_Identity=35.2760736196319, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=30.5239179954442, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=30.5239179954442, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI54607086, Length=324, Percent_Identity=30.8641975308642, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI194018522, Length=437, Percent_Identity=26.7734553775744, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI194018520, Length=437, Percent_Identity=26.7734553775744, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI194097354, Length=437, Percent_Identity=26.7734553775744, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI46094014, Length=436, Percent_Identity=27.0642201834862, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4503507, Length=279, Percent_Identity=29.7491039426523, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=35.5555555555556, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=63.1313131313131, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=63.1313131313131, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI2367247, Length=337, Percent_Identity=30.5637982195846, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789108, Length=355, Percent_Identity=28.169014084507, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI48994988, Length=237, Percent_Identity=29.957805907173, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788922, Length=141, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=49.7512437810945, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=40.4145077720207, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17552884, Length=334, Percent_Identity=35.9281437125749, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17569207, Length=334, Percent_Identity=35.9281437125749, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI32566303, Length=304, Percent_Identity=34.8684210526316, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=30.1470588235294, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=27.5462962962963, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=27.5462962962963, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=460, Percent_Identity=25, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=39.3103448275862, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=457, Percent_Identity=26.9146608315098, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=241, Percent_Identity=28.6307053941909, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=56.6750629722922, Blast_Score=444, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6325337, Length=325, Percent_Identity=36.6153846153846, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=325, Percent_Identity=36.6153846153846, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=456, Percent_Identity=25.219298245614, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=341, Percent_Identity=29.0322580645161, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=278, Percent_Identity=30.9352517985612, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=272, Percent_Identity=25, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363316, Length=392, Percent_Identity=53.0612244897959, Blast_Score=433, Evalue=1e-122,
Organism=Drosophila melanogaster, GI17864358, Length=392, Percent_Identity=53.0612244897959, Blast_Score=433, Evalue=1e-122,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=47.9381443298969, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI45553807, Length=332, Percent_Identity=33.7349397590361, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45553816, Length=332, Percent_Identity=33.7349397590361, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24651721, Length=332, Percent_Identity=33.7349397590361, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI17864154, Length=332, Percent_Identity=33.7349397590361, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24652838, Length=332, Percent_Identity=34.0361445783133, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17137572, Length=332, Percent_Identity=34.0361445783133, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.0457516339869, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI45550900, Length=346, Percent_Identity=29.4797687861272, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI17137380, Length=441, Percent_Identity=26.530612244898, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24647046, Length=369, Percent_Identity=30.0813008130081, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24647048, Length=369, Percent_Identity=30.0813008130081, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43399; Mature: 43268

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEVFQRTKPHMNVGTIGHVDHGKTTLTAAISIYCSKLNKDAKALKYEDIDNAPEEKAR
CCHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHHHC
GITINARHIEYETANRHYAHVDCPGHADYIKNMITGAAQMDAAILLVAADSGAEPQTKEH
CEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEEECCCCCCCHHHH
LLLAQRMGIKKIIVFLNKLDLADPELVELVEVEVLELVEKYGFSADTPIIKGSAFGAMSN
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
PEDPESTKCVKELLESMDNYFDLPERDIDKPFLLAVEDVFSISGRGTVATGRIERGIIKV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHCCCCCEEEECHHHHHHHHC
GQEVEIVGIKETRKTTVTGVEMFQKILEQGQAGDNVGLLLRGVDKKDIERGQVLSAPGTI
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEECCCCCC
TPHKKFKASIYCLTKEEGGRHKPFFPGYRPQFFFRTTDVTGVVALEGKEMVMPGDNVDII
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCEEECCCCCHHHH
VELISSIAMDKNVEFAVREGGRTVASGRILEILE
HHHHHHHHCCCCCEEEEECCCCEEECCCEEEECC
>Mature Secondary Structure 
AKEVFQRTKPHMNVGTIGHVDHGKTTLTAAISIYCSKLNKDAKALKYEDIDNAPEEKAR
CHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHHHC
GITINARHIEYETANRHYAHVDCPGHADYIKNMITGAAQMDAAILLVAADSGAEPQTKEH
CEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEEECCCCCCCHHHH
LLLAQRMGIKKIIVFLNKLDLADPELVELVEVEVLELVEKYGFSADTPIIKGSAFGAMSN
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
PEDPESTKCVKELLESMDNYFDLPERDIDKPFLLAVEDVFSISGRGTVATGRIERGIIKV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHCCCCCEEEECHHHHHHHHC
GQEVEIVGIKETRKTTVTGVEMFQKILEQGQAGDNVGLLLRGVDKKDIERGQVLSAPGTI
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEECCCCCC
TPHKKFKASIYCLTKEEGGRHKPFFPGYRPQFFFRTTDVTGVVALEGKEMVMPGDNVDII
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCEEECCCCCHHHH
VELISSIAMDKNVEFAVREGGRTVASGRILEILE
HHHHHHHHCCCCCEEEEECCCCEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA