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The map label for this gene is yibK [C]

Identifier: 161510073

GI number: 161510073

Start: 1988649

End: 1989119

Strand: Reverse

Name: yibK [C]

Synonym: USA300HOU_1850

Alternate gene names: 161510073

Gene position: 1989119-1988649 (Counterclockwise)

Preceding gene: 161510074

Following gene: 161510072

Centisome position: 69.24

GC content: 31.21

Gene sequence:

>471_bases
ATGACAAATCATATCGTTTTATATCAACCAGAAATCCCAGCTAATACTGGTAACATTGCACGTACGTGTGCAGGTACAAA
CACACATTTACATTTAATTAAACCTCTTGGATTTAGAACTGATGATAAAATGTTAAAAAGAGCCGGTTTAGATTATTGGG
AATTTGTGAATATTACGTATCATGATAGTATTGAAGCATTTTTTGAAGTCACAAATGGCGAGTATTACTTATTAACAAAA
TTTGGTAAAAAGACTTATAGTGATTTTGATTTTTCAAATCATGACAAAGATTTTTACTTCATTTTTGGAAAAGAAACGAC
AGGATTACCAGACTGGGTGAAAGAGAAATATCAAGACACAGCGTTAAGAATTCCTATGAGTGAACATATTCGTTCATTAA
ATTTATCAAATACTGCTGCGTTGTTAATTTATGAAGCGTTACGTCAACAAGATTTTCCGGGATTGAATTAA

Upstream 100 bases:

>100_bases
GAAGAAGCAAGAGATTTTATCAATGCTAATTATGATCAAGAGGATGCAGAAGTTCAGAATGAAATGATAAAAGGATTAGA
CACAAGGAGAGAATAGTAAT

Downstream 100 bases:

>100_bases
AATTTACTCATTTTCATTATAAAAATGATGCTTAAGTGCTATATTTAGAGTATAAGCATGTTAAAGTTAAATGGTTTGAT
ATATTGAAAGTTAGGGTATA

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTK
FGKKTYSDFDFSNHDKDFYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN

Sequences:

>Translated_156_residues
MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTK
FGKKTYSDFDFSNHDKDFYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN
>Mature_155_residues
TNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKF
GKKTYSDFDFSNHDKDFYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=46.4052287581699, Blast_Score=137, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18111; Mature: 17979

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITY
CCCCEEEECCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCHHEEEEEE
HDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIFGKETTGLPDWVKEKYQDT
CCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCC
ALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN
EEECCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITY
CCCEEEECCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCHHEEEEEE
HDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIFGKETTGLPDWVKEKYQDT
CCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCC
ALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN
EEECCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]