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The map label for this gene is rot

Identifier: 161509980

GI number: 161509980

Start: 1891506

End: 1892006

Strand: Reverse

Name: rot

Synonym: USA300HOU_1753

Alternate gene names: 161509980

Gene position: 1892006-1891506 (Counterclockwise)

Preceding gene: 161509981

Following gene: 161509977

Centisome position: 65.86

GC content: 31.34

Gene sequence:

>501_bases
ATGCATAAGTTAGCACATACAAGTTTTGGGATTGTTGGGATGTTTGTTAATACTTGTATAGTAGCTAAATATGTGATTAT
TAATTGGGAGATGTTTAGCATGAAAAAAGTAAATAACGACACTGTATTTGGAATTTTGCAATTAGAAACACTTTTGGGTG
ACATTAACTCAATTTTCAGCGAGATTGAAAGCGAATACAAAATGTCTAGAGAAGAAATTTTAATTTTACTAACTTTATGG
CAAAAAGGTTCTATGACGCTTAAAGAAATGGACAGATTTGTTGAAGTTAAACCGTATAAGCGTACGAGAACGTATAATAA
TTTAGTTGAATTAGAATGGATTTACAAAGAGCGTCCTGTTGACGATGAAAGAACAGTTATTATTCATTTCAATGAAAAGT
TACAACAAGAGAAAGTAGAGTTGTTGAATTTCATCAGTGATGCGATTGCAAGTAGAGCAACAGCAATGCAAAATAGTTTA
AACGCAATTATTGCTGTGTAA

Upstream 100 bases:

>100_bases
CTAAATATTAACTCTATTGATTATTGGTTTTTATACTTATTTAATTTTATTCAACTTTGACAATTGAATAGAAAGCAAGT
TTATTTACACTTGTAGTTTT

Downstream 100 bases:

>100_bases
GTTTAATAGCATAAAAAGAGGTTTTCATTAAGTTGAAAACCTCTTTTTGTTGTTGGCATTAATTTTTCAAATGTTGACTA
CTCAATCCTAAATTATAAAT

Product: repressor of toxins Rot

Products: NA

Alternate protein names: Repressor of toxins

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MHKLAHTSFGIVGMFVNTCIVAKYVIINWEMFSMKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILLTLW
QKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQQEKVELLNFISDAIASRATAMQNSL
NAIIAV

Sequences:

>Translated_166_residues
MHKLAHTSFGIVGMFVNTCIVAKYVIINWEMFSMKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILLTLW
QKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQQEKVELLNFISDAIASRATAMQNSL
NAIIAV
>Mature_166_residues
MHKLAHTSFGIVGMFVNTCIVAKYVIINWEMFSMKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILLTLW
QKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQQEKVELLNFISDAIASRATAMQNSL
NAIIAV

Specific function: Global regulator with both positive and negative effects that mediates modulation of several genes involved in virulence. Negatively regulates the transcription of several known virulence factors such as lipase (geh), hemolysins (hla and hlb) and protease

COG id: COG1846

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rot family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ROT_STAA8 (Q9RFJ6)

Other databases:

- EMBL:   AF189239
- EMBL:   CP000253
- RefSeq:   YP_500382.1
- ProteinModelPortal:   Q9RFJ6
- STRING:   Q9RFJ6
- EnsemblBacteria:   EBSTAT00000028144
- GeneID:   3921768
- GenomeReviews:   CP000253_GR
- KEGG:   sao:SAOUHSC_01879
- eggNOG:   COG1846
- GeneTree:   EBGT00050000023836
- HOGENOM:   HBG692942
- ProtClustDB:   CLSK885542
- BioCyc:   SAUR93061:SAOUHSC_01879-MONOMER
- GO:   GO:0005622
- GO:   GO:0009405
- InterPro:   IPR010166
- InterPro:   IPR000835
- InterPro:   IPR016998
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PIRSF:   PIRSF032474
- TIGRFAMs:   TIGR01889

Pfam domain/function: PF01047 MarR

EC number: NA

Molecular weight: Translated: 19371; Mature: 19371

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKLAHTSFGIVGMFVNTCIVAKYVIINWEMFSMKKVNNDTVFGILQLETLLGDINSIFS
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEHEEEECCCCEEEHHHHHHHHHHHHHHHHH
EIESEYKMSREEILILLTLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPV
HHHHHHHCCHHHEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEEEECCCCC
DDERTVIIHFNEKLQQEKVELLNFISDAIASRATAMQNSLNAIIAV
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
>Mature Secondary Structure
MHKLAHTSFGIVGMFVNTCIVAKYVIINWEMFSMKKVNNDTVFGILQLETLLGDINSIFS
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEHEEEECCCCEEEHHHHHHHHHHHHHHHHH
EIESEYKMSREEILILLTLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPV
HHHHHHHCCHHHEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEEEECCCCC
DDERTVIIHFNEKLQQEKVELLNFISDAIASRATAMQNSLNAIIAV
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10809700; 12511508