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The map label for this gene is recN [H]

Identifier: 161509748

GI number: 161509748

Start: 1636874

End: 1638553

Strand: Reverse

Name: recN [H]

Synonym: USA300HOU_1521

Alternate gene names: 161509748

Gene position: 1638553-1636874 (Counterclockwise)

Preceding gene: 161509749

Following gene: 161509747

Centisome position: 57.03

GC content: 30.65

Gene sequence:

>1680_bases
ATGTTACAAACCTTATCAATCAAGCAATTTGCTATTATTGAAGAATTAGAAATTCAATTTTCTGATGGTTTAACTGTTTT
AAGTGGTGAAACGGGTTCAGGTAAATCAATTATTATAGATGCAATTGGACAATTAATTGGAATGAGAGCTTCATCTGATT
TTGTAAGACATGGTGAAAAAAAGGCTGTCATCGAAGGTATATTTGATATTGACGAGAGTAAAGATGCTATACATATATTG
AAAAATATGGATATTGATGTAGATGAAGATTTTTTATTAGTTAAACGGGAAATTTTCAGCTCTGGTAAAAGTCTTTGTAA
AATTAATAATCAAACAGTTACTTTACAAGATTTACGAAAAGTTATGCAAGAATTATTAGATATTCATGGCCAACATGAAA
CGCAGTCTTTGTTGAAACAAAAATACCATTTAACATTATTGGATAATTACGCAGAATCACGTTATCAAGATTTGTTGGAT
AAATATCACCAAACTTTTCAAAATTATAAAGCCAAAAAGCAAGAGTTAGAAGATTTAGAATCAGCAGACCAAGCATTGCT
ACAGCGTTTAGATTTAATGAAATTCCAATTAGAAGAATTGTCTGAAGCACATCTAAAAGAAGGCGAAATAGAGCAACTTG
AAATAGATATTAAACGCATTCAAAACTCTGAAAAATTAAGCTTAGCGCTTAATAACGCACATATGACATTAACGGATGAA
AATGCTATCACTGATCGTTTATATGAACTAAGCAACCATTTGTTAACAATAAACGATATAGTTCCAAACAAATATGACAA
ATTAAAAGAAGATATTGATCAATTTTACTACATTTTAGAAGATGCAAAACATGAGTTATATGATGAAATGGCTAATACAG
AATTCGACGAACAAGTGTTAAACGAGTATGAATCTCGTATGAATTTGCTGAATAATTTAAAACGTAAATATGGAAAAGAT
ATTTCAGAATTAATCGCATATCAAGAAAAGCTTAATAATGAAATCAATAAAATTGAAAACTATGAACAAAGTACATCGCA
GTTACGAGAAGAAATAAATGCATTGTATAATCAAGTTATAGAGGTTGGACAAGCGTTATCAAAGCAGCGTCGTATAGTCG
CTAGGGAGTTAAGAGACCACATTGTATCGGAAATTCAAAACTTACAAATGAAAGACGCAAATCTTGAGATTTCATTTAAA
AAATTAGAAGAACCGAATATTGATGGAATCGAATTTGTAGAATTTTTAATCAGTCCAAATAAAGGGGAACCATTAAAAAG
TTTAAATAAAATTGCGTCAGGTGGAGAACTTTCTAGAATTATGTTAGCGTTAAAAAGTATTTTTGTTAAATCGAGAGGTC
AAACTGCAATTTTATTCGATGAGGTTGACTCAGGTGTATCTGGACAAGCTGCACAAAAAATGGCAGAGAAAATGCGAGAT
ATAGCAGAATATATACAAGTTATTTGTATCTCTCACTTACCACAAGTTGCATCGATGAGCGACCATCATTTATTAATTTC
GAAATCATCAAAAGATGATCGTACAACAACACAAGTTCAAGAGTTAATTAGCGATGATAAAGTAGATGAAATTGCACGAA
TGATTTCAGGTGCAAGTGTTACTGATTTAACTCGAGAAAATGCAAGAGAAATGATACAACACAATCAAAGACGTAGATAA

Upstream 100 bases:

>100_bases
TTGTGGTGATGATACTTGTTTAATTATTTGTCGAAGCAAAGAGGCAAGTGATGAAATCAAGTCAAGAATTTTCAATTTGT
TATAAGGATGCGATGAGTTT

Downstream 100 bases:

>100_bases
TTTCCAAAGTGTGTTGAATACTCATTTATGGATTTATTTATGTGCATTCAAACTAAATAGACAATCATGTAAAATAGGTA
ATTAATGGACATGAAAACTA

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHIL
KNMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDE
NAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKD
ISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKDANLEISFK
KLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRD
IAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR

Sequences:

>Translated_559_residues
MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHIL
KNMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDE
NAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKD
ISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKDANLEISFK
KLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRD
IAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR
>Mature_559_residues
MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHIL
KNMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDE
NAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKD
ISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKDANLEISFK
KLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRD
IAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=562, Percent_Identity=34.3416370106762, Blast_Score=289, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 64297; Mature: 64297

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEK
CCCCCCHHHHHHHHHHHHEECCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCH
KAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRK
HHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHEEECCCEEEHHHHHH
VMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLDKYHQTFQNYKAKKQELEDLE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCEEEECCC
NAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVL
HHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NEYESRMNLLNNLKRKYGKDISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGQALSKQRRIVARELRDHIVSEIQNLQMKDANLEISFKKLEEPNIDGIEFVEFLISPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHCCCCCCCCHHHHHHHHHCCC
KGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRD
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
IAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELISDDKVDEIARMISGASV
HHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCH
TDLTRENAREMIQHNQRRR
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEK
CCCCCCHHHHHHHHHHHHEECCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCH
KAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRK
HHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHEEECCCEEEHHHHHH
VMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLDKYHQTFQNYKAKKQELEDLE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCEEEECCC
NAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVL
HHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NEYESRMNLLNNLKRKYGKDISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGQALSKQRRIVARELRDHIVSEIQNLQMKDANLEISFKKLEEPNIDGIEFVEFLISPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHCCCCCCCCHHHHHHHHHCCC
KGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRD
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
IAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELISDDKVDEIARMISGASV
HHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCH
TDLTRENAREMIQHNQRRR
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]