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The map label for this gene is bmfBB [H]

Identifier: 161509744

GI number: 161509744

Start: 1632024

End: 1633298

Strand: Reverse

Name: bmfBB [H]

Synonym: USA300HOU_1517

Alternate gene names: 161509744

Gene position: 1633298-1632024 (Counterclockwise)

Preceding gene: 161509745

Following gene: 161509743

Centisome position: 56.85

GC content: 33.18

Gene sequence:

>1275_bases
ATGGAAATAACAATGCCTAAGTTAGGTGAGAGTGTTCATGAAGGCACCATTGAACAATGGTTAGTTTCTGTTGGTGATCA
TATTGATGAATATGAACCATTATGTGAAGTTATTACAGATAAAGTGACAGCTGAAGTCCCTTCCACGATATCAGGAACAA
TTACAGAAATTTTAGTTGAAGCGGGGCAGACAGTAGCTATTGATACAATTATCTGTAAAATTGAAACTGCTGATGAAAAG
ACAAATGAAACAACTGAAGAGATACAAGCAAAAGTGGATGAGCATACTCAGAAATCTACTAAAAAAGCTAGTGCAACAGT
GGAACAGACATCTACTGCTAAACAAAATCAACCACGTAATAATGGTCGCTTTTCACCTGTTGTATTTAAACTCGCTTCAG
AGCATGACATTGATTTATCACAAGTTGTAGGTAGTGGATTTGAAGGTCGTGTAACTAAGAAGGATATAATGTCAGTTATT
GAAAATGGTGGTACCACAGCTCAATCTGACAAACAAGTTCAAACAAAATCAACATCAGTAGATACATCAAGTAACCAATC
ATCTGAAGACAATAGTGAAAACAGCACAATACCAGTAAATGGTGTGCGTAAAGCAATTGCGCAAAATATGGTTAATAGTG
TAACAGAGATTCCACATGCATGGATGATGATTGAAGTAGATGCTACAAATCTTGTGAATACGAGAAATCATTATAAAAAC
AGCTTTAAAAATAAAGAAGGATATAATCTAACGTTCTTTGCTTTCTTTGTAAAAGCTGTAGCAGATGCTTTAAAAGCATA
TCCTTTATTAAATAGTAGCTGGCAAGGAAATGAAATTGTCTTACATAAAGACATTAATATTTCAATTGCTGTTGCTGATG
AAAATAAATTATACGTACCTGTGATTAAGCATGCAGACGAAAAGTCAATCAAAGGTATAGCTAGAGAAATTAATACTTTA
GCAACGAAAGCGCGTAATAAGCAATTGACAGCTGAAGATATGCAGGGCGGTACATTTACGGTAAATAATACTGGTACATT
TGGTTCAGTATCATCAATGGGTATTATAAATCATCCACAAGCAGCGATTTTACAAGTAGAATCAATCATTAAAAAGCCAG
TAGTAATTAATGATATGATTGCAATTCGTAACATGGTTAATTTATGTATTTCAATTGATCATCGTATTTTAGATGGTTTA
CAAACAGGTAAATTTATGAATCATATTAAACAGCGTATCGAACAGTATACTTTAGAAAATACAAATATATATTAG

Upstream 100 bases:

>100_bases
TATGCCATTTTCTCCTGTATTAGAAAATGAAATTATGATGAATCCAGAAAAAATCTTAAATAAAATGCGTGAATTAGCAG
AATTCTAGGGAGGGAAAGTC

Downstream 100 bases:

>100_bases
TGATAACATAGATGCATCTATCGACAACTTGTTTTATCTTGTTCTTGTCGATGGATGTATTATTTTTTGGCACTAAAATA
TGTGCAATATATTCAAAAAA

Product: dihydrolipoyllysine-residue acetyltransferase

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTAEVPSTISGTITEILVEAGQTVAIDTIICKIETADEK
TNETTEEIQAKVDEHTQKSTKKASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIPHAWMMIEVDATNLVNTRNHYKN
SFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQGNEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTL
ATKARNKQLTAEDMQGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISIDHRILDGL
QTGKFMNHIKQRIEQYTLENTNIY

Sequences:

>Translated_424_residues
MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTAEVPSTISGTITEILVEAGQTVAIDTIICKIETADEK
TNETTEEIQAKVDEHTQKSTKKASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIPHAWMMIEVDATNLVNTRNHYKN
SFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQGNEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTL
ATKARNKQLTAEDMQGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISIDHRILDGL
QTGKFMNHIKQRIEQYTLENTNIY
>Mature_424_residues
MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTAEVPSTISGTITEILVEAGQTVAIDTIICKIETADEK
TNETTEEIQAKVDEHTQKSTKKASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIPHAWMMIEVDATNLVNTRNHYKN
SFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQGNEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTL
ATKARNKQLTAEDMQGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISIDHRILDGL
QTGKFMNHIKQRIEQYTLENTNIY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=432, Percent_Identity=28.2407407407407, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI110671329, Length=427, Percent_Identity=29.5081967213115, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI203098753, Length=448, Percent_Identity=27.0089285714286, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI203098816, Length=448, Percent_Identity=27.0089285714286, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI19923748, Length=218, Percent_Identity=36.697247706422, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI260898739, Length=154, Percent_Identity=34.4155844155844, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1786946, Length=417, Percent_Identity=30.6954436450839, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1786305, Length=421, Percent_Identity=27.790973871734, Blast_Score=151, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=429, Percent_Identity=27.5058275058275, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=29.9319727891156, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=224, Percent_Identity=34.375, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=326, Percent_Identity=26.0736196319018, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=414, Percent_Identity=29.951690821256, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324258, Length=448, Percent_Identity=27.2321428571429, Blast_Score=139, Evalue=6e-34,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=28.7081339712919, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24645909, Length=190, Percent_Identity=37.8947368421053, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24582497, Length=221, Percent_Identity=31.2217194570136, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI20129315, Length=221, Percent_Identity=31.2217194570136, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 46740; Mature: 46740

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTAEVPSTISGTITEILVE
CCCCCCHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKKASATVEQTSTAKQNQPRN
CCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVIENGGTTAQSDKQVQTKSTSV
CCCCCHHEEEECCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCC
DTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIPHAWMMIEVDATNLVNTRNHYKN
CCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHH
SFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQGNEIVLHKDINISIAVADENKLYVP
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEEEECCCEEEEE
VIKHADEKSIKGIAREINTLATKARNKQLTAEDMQGGTFTVNNTGTFGSVSSMGIINHPQ
EECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCCCCCCCCCCCCCCH
AAILQVESIIKKPVVINDMIAIRNMVNLCISIDHRILDGLQTGKFMNHIKQRIEQYTLEN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TNIY
CCCC
>Mature Secondary Structure
MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTAEVPSTISGTITEILVE
CCCCCCHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKKASATVEQTSTAKQNQPRN
CCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVIENGGTTAQSDKQVQTKSTSV
CCCCCHHEEEECCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCC
DTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIPHAWMMIEVDATNLVNTRNHYKN
CCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHH
SFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQGNEIVLHKDINISIAVADENKLYVP
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEEEECCCEEEEE
VIKHADEKSIKGIAREINTLATKARNKQLTAEDMQGGTFTVNNTGTFGSVSSMGIINHPQ
EECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCCCCCCCCCCCCCCH
AAILQVESIIKKPVVINDMIAIRNMVNLCISIDHRILDGLQTGKFMNHIKQRIEQYTLEN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TNIY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]