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The map label for this gene is dus

Identifier: 161508354

GI number: 161508354

Start: 97363

End: 98349

Strand: Reverse

Name: dus

Synonym: USA300HOU_0096

Alternate gene names: 161508354

Gene position: 98349-97363 (Counterclockwise)

Preceding gene: 161508357

Following gene: 161508353

Centisome position: 3.42

GC content: 38.91

Gene sequence:

>987_bases
ATGAAAGAAAATTTTTGGAGTGAATTACCACGTCCATTTTTTATTTTGGCGCCAATGGAAGACGTTACAGATATCGTCTT
TCGACACGTTGTAAGTGAAGCAGCTAGACCGGATGTGTTTTTCACTGAATTTACAAATACTGAAAGCTTTTGCCACCCTG
AAGGCATACATAGTGTGCGCGGACGCTTAACTTTTAGTGAAGATGAACAGCCGATGGTCGCTCATATATGGGGAGATAAG
CCAGAACAGTTCCGTGAAACGAGTATTCAATTAGCTAAAATGGGCTTTAAAGGCATAGACTTAAATATGGGATGTCCTGT
AGCAAATGTTGCTAAAAAGGGTAAGGGTTCCGGCTTAATCTTAAGACCTGACGTTGCTGCCGAAATTATTCAAGCGACTA
AAGCAGGTGGGCTTCCGGTAAGTGTTAAAACACGCCTTGGCTACTATGAAATCGATGAATGGAAAGATTGGTTGAAGCAC
GTCTTCGAACAAGACATTGCCAATTTATCTATTCATCTTCGTACACGTAAAGAAATGAGTAAAGTAGATGCACATTGGGA
ATTAATCGAAGCTATTAAAAATTTACGTGACGAAATTGCACCAAATACATTGTTAACAATTAACGGTGATATTCCCGATA
GAAAAACAGGACTTGAACTGGCAGAAAAATATGGCATTGATGGCGTCATGATTGGTAGAGGCATTTTCCACAATCCATTC
GCTTTTGAAAAAGAACCACGCGAACACACAAGCAAGGAACTATTAGATCTATTGAGATTGCATTTATCATTGTTTAACAA
ATATGAAAAAGATGAAATACGACAATTCAAGAGCTTGCGTAGATTCTTTAAAATCTATGTGCGTGGCATAAGAGGCGCTA
GCGAACTTCGACATCAATTGATGAACACACAATCAATTGCAGAAGCACGAGCACTACTCGATGAATTTGAAGCCCAAATG
GACGAAGACGTTAAAATTGAATTATAG

Upstream 100 bases:

>100_bases
GCATCTATTGAAATTCCTTTTGTATAAGTTAGGCTTTTGTGGTAATATCATCATGCATAAAAAATCGAGATACTAATTAT
AAAGAGGGTATAAATATATT

Downstream 100 bases:

>100_bases
TATGAGTAATAAAGTTTATGGATGATATTTCCCAATTTAACACGGATTGAACACTTTCTACTTAGGTATTATCTTGGTTT
TCCTGATAGGATAACTCCCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDK
PEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQM
DEDVKIEL

Sequences:

>Translated_328_residues
MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDK
PEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQM
DEDVKIEL
>Mature_328_residues
MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDK
PEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQM
DEDVKIEL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family

Homologues:

Organism=Homo sapiens, GI40807366, Length=328, Percent_Identity=23.4756097560976, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789660, Length=254, Percent_Identity=28.3464566929134, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI145693211, Length=252, Percent_Identity=27.3809523809524, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=248, Percent_Identity=28.2258064516129, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=172, Percent_Identity=29.6511627906977, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=324, Percent_Identity=24.0740740740741, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=236, Percent_Identity=25, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921524, Length=235, Percent_Identity=27.6595744680851, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24580595, Length=306, Percent_Identity=25.8169934640523, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920448, Length=306, Percent_Identity=25.8169934640523, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUS_STAAC (Q5HJT5)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_184972.1
- ProteinModelPortal:   Q5HJT5
- SMR:   Q5HJT5
- STRING:   Q5HJT5
- EnsemblBacteria:   EBSTAT00000009073
- GeneID:   3236888
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL0067
- TIGR:   SACOL0067
- eggNOG:   COG0042
- GeneTree:   EBGT00050000024100
- HOGENOM:   HBG352957
- OMA:   VVFRHVV
- ProtClustDB:   CLSK865525
- BioCyc:   SAUR93062:SACOL0067-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 37768; Mature: 37768

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
CCCCCHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHH
GRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
CCEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCEE
LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
ECCHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHCEEEEEECHHHHH
KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
MNTQSIAEARALLDEFEAQMDEDVKIEL
HHHHHHHHHHHHHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
CCCCCHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHH
GRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
CCEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCEE
LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
ECCHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHCEEEEEECHHHHH
KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
MNTQSIAEARALLDEFEAQMDEDVKIEL
HHHHHHHHHHHHHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA