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The map label for this gene is thiC

Identifier: 161486848

GI number: 161486848

Start: 877273

End: 878589

Strand: Direct

Name: thiC

Synonym: CTC00828

Alternate gene names: 161486848

Gene position: 877273-878589 (Clockwise)

Preceding gene: 28210544

Following gene: 28210546

Centisome position: 31.34

GC content: 32.5

Gene sequence:

>1317_bases
ATGAATTATACAACTCAAATGGATGCTGCTAAAAGAGGAATAGTTACCAAGGAAATGGAAACTATAGCTAAAAAAGAAGG
CATAGATGTAAAAAAAATAATAGAATGTGTAGCAAAAGGTAGTGTTGCTATACCTGCAAATAAAAATCATAAAAGTTTAA
GTGCCGAAGGAGTAGGACAAGGGCTAAGAACTAAAATAAATGTAAATCTAGGTATATCAAAAGATTGTTGTAATGTAGAT
AGAGAACTTGAAAAAGTAAAAAAAGCTATAGATATGAAGGCTGAAGCCATAATGGATTTAAGTTGTTTTGGAAAGACACA
AGAATTTAGGAAAAGATTAATAGAAATGTCACCAGCCATGATAGGAACTGTTCCAATGTATGATGCTATTGGATTTTATG
ATAAAGAGTTAAAAGATATTACCGCAGAAGAATTGCTAGATGTAATAGAAACTCATGCAAAAGATGGAGTAGACTTTATG
ACTATCCACGCAGGACTTAACAGAGAAGCTGTGGAAATATTTAAGAGAAATGAAAGACTTACTAACATAGTATCCAGAGG
AGGGTCTCTTCTTTATGCATGGATGGAACTTAATAATAAGGAAAATCCTTTCTATGAACATTTTGATAAAGTACTCGATA
TATGTGAAAAATATGATGTTACAATAAGCTTAGGAGATGCTTGTAGACCAGGTTCAATACATGATTCTACAGATGCAAGC
CAAATAAAAGAACTTATGACTTTAGGGGAATTAACTAAAAGGGCATGGGAAAGAAATGTTCAAATTATAATAGAAGGACC
TGGACACATGTCCTTAGATGAAATAGAAACAAATATGAAATTAGAAAAAAAATTATGTTATAATGCACCTTTTTATGTAT
TGGGGCCTATAGTTACAGATATAGCTCCTGGATATGATCATATTACAAGTGCTATAGGAGGAGCAATTGCTGCAACTTAT
GGAGCAGACTTTTTATGCTATGTAACTCCAGCAGAACACTTAAGACTTCCTGATTTAGATGATATGAAAGAAGGTATAAT
AGCAACTAAAATAGCAGCTCATGCAGCAGATATTGCTAAAGGAATAAAAGGGGCAAGAGAATGGGATAATAAAATGTCCG
TTGCAAGAAAAGAGTTAGATTGGGAAAAGATGTTTGATTTGGCTATAGATAATGAGAAGGCAAGAAAGTATAGAAAGAAT
TCAACACCAGAGGTTAACGATAGTTGTACAATGTGTGGAAAAATGTGTGCTGTTAGAAATATGAATAAGGTTATGCAAGG
AAAAGATATAAATATATTGAGAAACGAGGATAAATAA

Upstream 100 bases:

>100_bases
TAGTTATGGCATAAGTCCATAACAATATCTATACATATGTATTTTGAGGGAAAGTTTAAAACTTTCCCTTTTTTATTTTA
ATAAAAAGGAGAGGAATAAA

Downstream 100 bases:

>100_bases
AAATTTTGCGTGACAAAATTTTTATTTAATGAACAAGGAATAATGAATAATGAACAATTGTGGATATTTTTTCTCCATTA
ACATTACGAAAAAATTTTAA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MNYTTQMDAAKRGIVTKEMETIAKKEGIDVKKIIECVAKGSVAIPANKNHKSLSAEGVGQGLRTKINVNLGISKDCCNVD
RELEKVKKAIDMKAEAIMDLSCFGKTQEFRKRLIEMSPAMIGTVPMYDAIGFYDKELKDITAEELLDVIETHAKDGVDFM
TIHAGLNREAVEIFKRNERLTNIVSRGGSLLYAWMELNNKENPFYEHFDKVLDICEKYDVTISLGDACRPGSIHDSTDAS
QIKELMTLGELTKRAWERNVQIIIEGPGHMSLDEIETNMKLEKKLCYNAPFYVLGPIVTDIAPGYDHITSAIGGAIAATY
GADFLCYVTPAEHLRLPDLDDMKEGIIATKIAAHAADIAKGIKGAREWDNKMSVARKELDWEKMFDLAIDNEKARKYRKN
STPEVNDSCTMCGKMCAVRNMNKVMQGKDINILRNEDK

Sequences:

>Translated_438_residues
MNYTTQMDAAKRGIVTKEMETIAKKEGIDVKKIIECVAKGSVAIPANKNHKSLSAEGVGQGLRTKINVNLGISKDCCNVD
RELEKVKKAIDMKAEAIMDLSCFGKTQEFRKRLIEMSPAMIGTVPMYDAIGFYDKELKDITAEELLDVIETHAKDGVDFM
TIHAGLNREAVEIFKRNERLTNIVSRGGSLLYAWMELNNKENPFYEHFDKVLDICEKYDVTISLGDACRPGSIHDSTDAS
QIKELMTLGELTKRAWERNVQIIIEGPGHMSLDEIETNMKLEKKLCYNAPFYVLGPIVTDIAPGYDHITSAIGGAIAATY
GADFLCYVTPAEHLRLPDLDDMKEGIIATKIAAHAADIAKGIKGAREWDNKMSVARKELDWEKMFDLAIDNEKARKYRKN
STPEVNDSCTMCGKMCAVRNMNKVMQGKDINILRNEDK
>Mature_438_residues
MNYTTQMDAAKRGIVTKEMETIAKKEGIDVKKIIECVAKGSVAIPANKNHKSLSAEGVGQGLRTKINVNLGISKDCCNVD
RELEKVKKAIDMKAEAIMDLSCFGKTQEFRKRLIEMSPAMIGTVPMYDAIGFYDKELKDITAEELLDVIETHAKDGVDFM
TIHAGLNREAVEIFKRNERLTNIVSRGGSLLYAWMELNNKENPFYEHFDKVLDICEKYDVTISLGDACRPGSIHDSTDAS
QIKELMTLGELTKRAWERNVQIIIEGPGHMSLDEIETNMKLEKKLCYNAPFYVLGPIVTDIAPGYDHITSAIGGAIAATY
GADFLCYVTPAEHLRLPDLDDMKEGIIATKIAAHAADIAKGIKGAREWDNKMSVARKELDWEKMFDLAIDNEKARKYRKN
STPEVNDSCTMCGKMCAVRNMNKVMQGKDINILRNEDK

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=452, Percent_Identity=42.9203539823009, Blast_Score=364, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_CLOTE (Q897B2)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781489.2
- GeneID:   1060788
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00828
- NMPDR:   fig|212717.1.peg.711
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- PhylomeDB:   Q897B2
- ProtClustDB:   PRK13352
- BioCyc:   CTET212717:CTC_00828-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 48990; Mature: 48990

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: BINDING 88-88 BINDING 117-117 BINDING 146-146 BINDING 182-182 BINDING 284-284 BINDING 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTTQMDAAKRGIVTKEMETIAKKEGIDVKKIIECVAKGSVAIPANKNHKSLSAEGVGQ
CCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCCCCHHHCCC
GLRTKINVNLGISKDCCNVDRELEKVKKAIDMKAEAIMDLSCFGKTQEFRKRLIEMSPAM
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHH
IGTVPMYDAIGFYDKELKDITAEELLDVIETHAKDGVDFMTIHAGLNREAVEIFKRNERL
HCCCHHHHHHCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
TNIVSRGGSLLYAWMELNNKENPFYEHFDKVLDICEKYDVTISLGDACRPGSIHDSTDAS
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHH
QIKELMTLGELTKRAWERNVQIIIEGPGHMSLDEIETNMKLEKKLCYNAPFYVLGPIVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IAPGYDHITSAIGGAIAATYGADFLCYVTPAEHLRLPDLDDMKEGIIATKIAAHAADIAK
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GIKGAREWDNKMSVARKELDWEKMFDLAIDNEKARKYRKNSTPEVNDSCTMCGKMCAVRN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
MNKVMQGKDINILRNEDK
HHHHHCCCCCEEECCCCC
>Mature Secondary Structure
MNYTTQMDAAKRGIVTKEMETIAKKEGIDVKKIIECVAKGSVAIPANKNHKSLSAEGVGQ
CCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCCCCHHHCCC
GLRTKINVNLGISKDCCNVDRELEKVKKAIDMKAEAIMDLSCFGKTQEFRKRLIEMSPAM
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHH
IGTVPMYDAIGFYDKELKDITAEELLDVIETHAKDGVDFMTIHAGLNREAVEIFKRNERL
HCCCHHHHHHCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
TNIVSRGGSLLYAWMELNNKENPFYEHFDKVLDICEKYDVTISLGDACRPGSIHDSTDAS
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHH
QIKELMTLGELTKRAWERNVQIIIEGPGHMSLDEIETNMKLEKKLCYNAPFYVLGPIVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IAPGYDHITSAIGGAIAATYGADFLCYVTPAEHLRLPDLDDMKEGIIATKIAAHAADIAK
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GIKGAREWDNKMSVARKELDWEKMFDLAIDNEKARKYRKNSTPEVNDSCTMCGKMCAVRN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
MNKVMQGKDINILRNEDK
HHHHHCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129