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The map label for this gene is greB

Identifier: 161486671

GI number: 161486671

Start: 242674

End: 243156

Strand: Direct

Name: greB

Synonym: VV0235

Alternate gene names: 161486671

Gene position: 242674-243156 (Clockwise)

Preceding gene: 37678417

Following gene: 37678420

Centisome position: 7.23

GC content: 50.72

Gene sequence:

>483_bases
GTGAAAACCAAAATGATTACCCGTGAAGGGTACAACAAGCTCAAGCAAGAGCTCGATTATCTGTGGAAAGAGCACCGCCC
TGAGATCACGCAGAAAGTGTCGTGGGCGGCGAGCCTCGGCGATCGTTCAGAAAACGCCGACTACACCTACAACAAGCGTT
TGCTGCGTCAAATCGATCGCCGTGTGCGCTTTTTGAGTAAGTTTTTACCGGAAGTCAAAATCGTTGATTACGCACCGCAG
CAAGAGGGCAAAGTCTTTTTTGGTGCGTGGGTGGAAATTGAAAACGAAGCGGGCGAAGTGATGAAATTTCGCATCGTCGG
CCCGGAAGAGATTTACGGTGATGCCAAAGGCTACATTTCGATTGACTCGCCGATGGCGCGCGCGCTGCTCAAAAAAGAAG
TCGATGACGAGGTGCAAGTGCCAACCCCAAGCGGCATCAAAGAGTGGTTCATCAACTCGATTGAATACGACAAAGGGCAG
TGA

Upstream 100 bases:

>100_bases
AGAGTATTGCGTCAAATCTTCGCAGGTGACTGGCAAAGCGCGGCAAAACTCGTTACTTTTCGCTCCATCTGATTCCGTTC
GAGCCATTAAGGGAAAGTTC

Downstream 100 bases:

>100_bases
TTTCTCTTGTCACCTTTTCAAACCCGCCTCGTGCGGGTTTGTTTTATCTTAACGATTAGTGACGTACGACGTTGCAAGCG
GGTGGTGCATCCGCTAAAAG

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MKTKMITREGYNKLKQELDYLWKEHRPEITQKVSWAASLGDRSENADYTYNKRLLRQIDRRVRFLSKFLPEVKIVDYAPQ
QEGKVFFGAWVEIENEAGEVMKFRIVGPEEIYGDAKGYISIDSPMARALLKKEVDDEVQVPTPSGIKEWFINSIEYDKGQ

Sequences:

>Translated_160_residues
MKTKMITREGYNKLKQELDYLWKEHRPEITQKVSWAASLGDRSENADYTYNKRLLRQIDRRVRFLSKFLPEVKIVDYAPQ
QEGKVFFGAWVEIENEAGEVMKFRIVGPEEIYGDAKGYISIDSPMARALLKKEVDDEVQVPTPSGIKEWFINSIEYDKGQ
>Mature_160_residues
MKTKMITREGYNKLKQELDYLWKEHRPEITQKVSWAASLGDRSENADYTYNKRLLRQIDRRVRFLSKFLPEVKIVDYAPQ
QEGKVFFGAWVEIENEAGEVMKFRIVGPEEIYGDAKGYISIDSPMARALLKKEVDDEVQVPTPSGIKEWFINSIEYDKGQ

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=70.8860759493671, Blast_Score=230, Evalue=4e-62,
Organism=Escherichia coli, GI87082225, Length=159, Percent_Identity=35.2201257861635, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18712; Mature: 18712

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKMITREGYNKLKQELDYLWKEHRPEITQKVSWAASLGDRSENADYTYNKRLLRQIDR
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RVRFLSKFLPEVKIVDYAPQQEGKVFFGAWVEIENEAGEVMKFRIVGPEEIYGDAKGYIS
HHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEEECHHHHHCCCCCEEE
IDSPMARALLKKEVDDEVQVPTPSGIKEWFINSIEYDKGQ
ECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKTKMITREGYNKLKQELDYLWKEHRPEITQKVSWAASLGDRSENADYTYNKRLLRQIDR
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RVRFLSKFLPEVKIVDYAPQQEGKVFFGAWVEIENEAGEVMKFRIVGPEEIYGDAKGYIS
HHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEEECHHHHHCCCCCEEE
IDSPMARALLKKEVDDEVQVPTPSGIKEWFINSIEYDKGQ
ECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]