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The map label for this gene is gyrB [H]

Identifier: 161485752

GI number: 161485752

Start: 522837

End: 524789

Strand: Direct

Name: gyrB [H]

Synonym: SpyM3_0476

Alternate gene names: 161485752

Gene position: 522837-524789 (Clockwise)

Preceding gene: 21910011

Following gene: 21910013

Centisome position: 27.51

GC content: 39.78

Gene sequence:

>1953_bases
ATGATTGAAGAAAATAAACATTTTGAAAAAAAAATGCAAGAATACGATGCCAGTCAAATTCAGGTTCTAGAAGGGCTGGA
GGCTGTCCGCATGCGTCCAGGGATGTATATTGGTTCGACAGCTAAAGAGGGTTTGCATCATTTAGTCTGGGAAATTGTTG
ACAACTCAATTGACGAAGCATTAGCAGGTTTTGCCTCTCATATTAAAGTCTTTATTGAAGCAGATAATTCCATTACAGTA
GTAGATGATGGCCGTGGAATTCCAGTTGACATCCAAGTCAAGACAGGACGTCCCGCCGTTGAAACAGTTTTTACAGTCTT
ACACGCAGGTGGTAAATTTGGCGGAGGCAGCTATAAGGTTTCTGGAGGACTACATGGTGTAGGGTCATCTGTTGTTAATG
CTTTATCAACACAATTAGATGTACGTGTTTATAAAAACGGCCAAATTCATTACCAAGAATTTAAACGCGGGGCTGTTGTA
GCAGATCTTGAGGTCATTGGAACCACTGATGTGACTGGAACAACGGTACACTTTACACCCGATCCAGAAATTTTTACCGA
AACGACTCAGTTTGATTACAGTGTTTTAGCAAAACGTATTCAAGAGTTAGCCTTTTTAAACCGTGGTTTAAAAATTTCCA
TTACAGATAAGCGCTCAGGTATGGAACAAGAAGAACATTTCCATTATGAAGGTGGAATTGGTTCTTATGTTGAATTTTTA
AATGATAAAAAAGATGTTATCTTTGAAACGCCCATCTATACAGATGGTGAATTAGAAGGTATTGCAGTTGAAGTAGCCAT
GCAATACACGACTAGCTATCAAGAAACAGTCATGAGTTTTGCTAATAATATTCATACTCATGAAGGTGGAACGCATGAAC
AAGGCTTTAGAGCGGCTCTTACTCGGGTCATCAATGACTACGCTAAGAAAAATAAAATTCTTAAAGAAAATGAGGACAAT
TTGACAGGAGAAGATGTTCGTGAAGGTTTGACGGCGGTAATTTCTGTTAAGCATCCAAATCCTCAATTTGAAGGTCAAAC
CAAAACAAAATTGGGCAACTCAGAAGTGGTTAAGATCACTAATCGTCTCTTTAGTGAGGCCTTTCAACGTTTTCTTTTGG
AAAACCCACAAGTTGCTCGTAAGATTGTGGAAAAAGGGATTTTGGCTTCTAAAGCTAGAATTGCAGCTAAGCGAGCCCGT
GAAGTCACCCGCAAAAAATCAGGCTTAGAAATTTCAAACTTACCTGGAAAATTAGCAGACTGTTCGTCAAATGATGCTAA
CCAAAACGAACTTTTCATCGTCGAAGGAGATTCAGCGGGTGGGTCGGCCAAATCAGGTCGTAACCGAGAGTTTCAAGCTA
TCTTGCCTATTCGCGGTAAAATTTTGAACGTGGAAAAAGCAACTATGGATAAGATTCTTGCCAACGAAGAAATTAGAAGT
CTTTTTACCGCTATGGGTACAGGTTTTGGTGCAGACTTTGACGTGTCAAAAGCTCGCTACCAAAAGCTGGTTATCATGAC
CGATGCCGATGTGGATGGCGCTCATATTAGAACCTTGCTTTTAACTTTGATTTACCGCTTTATGAGGCCTGTTCTAGAAG
CTGGCTATGTTTACATCGCCCAGCCACCTATTTATGGTGTTAAGGTCGGTAGTGAGATTAAAGAGTATATTCAGCCAGGT
ATTGATCAAGAAGACCAATTAAAAACAGCTCTTGAAAAATATAGTATTGGTCGTTCAAAACCAACTGTTCAACGTTATAA
AGGTCTTGGGGAAATGGATGACCATCAACTTTGGGAAACTACTATGGATCCTGAAAATCGTTTGATGGCGCGTGTGACAG
TTGATGATGCCGCAGAAGCAGATAAAGTATTTGATATGTTAATGGGAGATCGTGTTGAACCAAGACGTGATTTCATTGAG
GAAAATGCGGTTTATAGTACACTGGATATTTAG

Upstream 100 bases:

>100_bases
AATTGGAGATCGGCTAATTGATAAACAAGCAGGACAAGCAGCGGGTTTTAATACCTTGCTTGTTGACGGTAGAAAAAACC
TATTGGAGATAGTAACGTAG

Downstream 100 bases:

>100_bases
TCACCTTTTTAGTTTCACAAGGAATCTGCTACCAAAGAGGACGAATTGACTAAAAATCATGATGCATGATGCCAACAGCT
TTGTTAGTTAGTACATCTTC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGLHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITV
VDDGRGIPVDIQVKTGRPAVETVFTVLHAGGKFGGGSYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGMEQEEHFHYEGGIGSYVEFL
NDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDN
LTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRAR
EVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRS
LFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPG
IDQEDQLKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPRRDFIE
ENAVYSTLDI

Sequences:

>Translated_650_residues
MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGLHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITV
VDDGRGIPVDIQVKTGRPAVETVFTVLHAGGKFGGGSYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGMEQEEHFHYEGGIGSYVEFL
NDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDN
LTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRAR
EVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRS
LFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPG
IDQEDQLKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPRRDFIE
ENAVYSTLDI
>Mature_650_residues
MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGLHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITV
VDDGRGIPVDIQVKTGRPAVETVFTVLHAGGKFGGGSYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGMEQEEHFHYEGGIGSYVEFL
NDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDN
LTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRAR
EVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRS
LFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPG
IDQEDQLKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPRRDFIE
ENAVYSTLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=684, Percent_Identity=26.4619883040936, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI19913406, Length=679, Percent_Identity=25.7731958762887, Blast_Score=119, Evalue=7e-27,
Organism=Escherichia coli, GI48994957, Length=564, Percent_Identity=54.0780141843972, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI1789408, Length=646, Percent_Identity=40.5572755417957, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17535065, Length=667, Percent_Identity=24.7376311844078, Blast_Score=129, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=552, Percent_Identity=23.731884057971, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=232, Percent_Identity=25.4310344827586, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=600, Percent_Identity=24.8333333333333, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17136538, Length=648, Percent_Identity=25.1543209876543, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72354; Mature: 72354

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGLHHLVWEIVDNSIDEA
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHH
LAGFASHIKVFIEADNSITVVDDGRGIPVDIQVKTGRPAVETVFTVLHAGGKFGGGSYKV
HHHHHHHEEEEEECCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVVADLEVIGTTDVTGTTVHFTP
CCCCCCHHHHHHHHHHHHEEEEEEECCCEEHHHHHCCCEEEEEEEEECCCCCCCEEEECC
DPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGMEQEEHFHYEGGIGSYVEFL
CCCCEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCEEECCCHHHHHHHH
NDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFANNIHTHEGGTHEQGFRAAL
CCCCCEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TRVINDYAKKNKILKENEDNLTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKIT
HHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHH
NRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRAREVTRKKSGLEISNLPGKLAD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCC
CSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRS
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHH
LFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIA
HHHHHCCCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEE
QPPIYGVKVGSEIKEYIQPGIDQEDQLKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWET
CCCCEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHC
TMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPRRDFIEENAVYSTLDI
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGLHHLVWEIVDNSIDEA
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHH
LAGFASHIKVFIEADNSITVVDDGRGIPVDIQVKTGRPAVETVFTVLHAGGKFGGGSYKV
HHHHHHHEEEEEECCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVVADLEVIGTTDVTGTTVHFTP
CCCCCCHHHHHHHHHHHHEEEEEEECCCEEHHHHHCCCEEEEEEEEECCCCCCCEEEECC
DPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGMEQEEHFHYEGGIGSYVEFL
CCCCEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCEEECCCHHHHHHHH
NDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFANNIHTHEGGTHEQGFRAAL
CCCCCEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TRVINDYAKKNKILKENEDNLTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKIT
HHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHH
NRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRAREVTRKKSGLEISNLPGKLAD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCC
CSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRS
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHH
LFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIA
HHHHHCCCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEE
QPPIYGVKVGSEIKEYIQPGIDQEDQLKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWET
CCCCEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHC
TMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPRRDFIEENAVYSTLDI
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]