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The map label for this gene is pheT

Identifier: 161484930

GI number: 161484930

Start: 700236

End: 701885

Strand: Direct

Name: pheT

Synonym: MTH770

Alternate gene names: 161484930

Gene position: 700236-701885 (Clockwise)

Preceding gene: 15678793

Following gene: 15678808

Centisome position: 39.98

GC content: 51.88

Gene sequence:

>1650_bases
ATGCCTGTTATAACGTTCGATTACGATGACCTGAAGGAACTGGGCATTGATATTGATAGGGAGAAGCTCCTTGACGTTCT
GCCGATGATGGGAAGTGATATAGAGGACTTTGATGACGAAAGCATTAAGGTGGAGTTTTTTCCCAACCGTCCTGACCTGC
TCTCGGTTGAGGGTGTTGCAAGGAGCCTCCGCGGATTCCTTGGAATTGAAAAGGGTATGCCATCCTATGATGTCCATGAC
TCCGGGGTGGAGGTCACAGTAGATGAGTCTGTACTTGATGTGAGGCCATACCTTGGAATGGCCGTCATAGAGGACGTTCA
GTTCACCGATAAAAAACTTAAACAGGTCATGGAGTTCCAGGAGGACCTGCACTGGGTCATAGGCAGGGACCGCAGGAAGG
TGGCCATAGGTATCCATGACCTTGACCGTGTCGAGCCACCCTTCCTCTACAGTGGCGTTGAACCGGAGGGAGTCACATTC
ACACCCCTTGACAGTGTATGTGAAATGACACCCCATGAAATACTCGAGGAGCACCCCAAGGGTGTCAGTTACGCCCACCT
CCTCCGGGACCATGACCGTTACCCCCTGATAACAGATAAGAATGGGGACGTTCTCTCCATGCCCCCCATAATAAATGGGG
AGCTCACCAAGTTAACCGTGAACACCCGCAGAATACTTGTGGATGTCACAGGAACCGATGATAGGGCTGTCAGACAGACC
CTGAATATCATATGCACATCCTTTGCAGAGGCGGGTGGAAGGATAGGCTCGGTGAGGGTTAAGCGTCCTGATGGAGAGCT
GCATCTACCAGACCTCACACCACGTGAGATGAGGGTATCTGTCTCAGAAGCCTCACGGATAACCGGACTTGAACTCAGTG
CAGATGAGGTCATGGATCTCCTCATGAAGGCCCGGATGGACGCCCGGAGGACCTCAGATGATGAGGTGGTTGCGGTTATA
CCCGCCTACCGGGTGGATATACTCCACGAGGTTGACCTTGTTGAGAACATAGCTGTGCAGCACTGCATAGGGAGGATTGA
ACCCGAACTTCCTGATATAGCGACCATTGCAGAGGAGGACACCTGGAGCAGGGCAGACGCGTCTATAAGGGAGGTCATGG
TGGGCCTCGGATTCCAGGAGGTTATGAGCCTCATGTTAACCAGTGAGGAGAGCCACTACAGGAGGATGAGGCTTGAGGAG
GATGAAAGGGTTGAGGTTGCCCAGCCCATTTCCCAAGACAGGACCATGATCCGCAAGAGTCTGCTCAACGGCCTCCTTGA
GTTCTTCGAGGACAACAAGCACGAGGATCTTCCACAGAAGATATTCGAGGTCGGGGATGTGGTGTACATTAACCCTGAAT
CTGAGACCCGTAGCAGGGTCGTTACGAAGCTTGCATGCGCAGTCACGCACTCCAGTGCAGGTTTCACCGAGATAAAGTCC
CTTGCAGCGGCTGTGGTTGAGAACCTCGGTTACGAGTTCAGGATAGAGCCCCTTGAACACCCATCCTTCATAGAGGGAAG
ATGTGCAGCCATAGAATCTGAGGGCAAATCCTCAGCCATAGGTGGATTCTTCGGTGAGGTCCATCCTGAGGTTGTAACAA
ACTTCAACCTGGAATATCCTGTTATTGCACTTGAAATCGAATTTAAGTAG

Upstream 100 bases:

>100_bases
CTCGTTGATGCAGCCCACACCCACGGACTCGCTGGTGGCGGTTAATTATTTATTTGATACCATGATAACATTCAAACATC
ACATTTACTGGAGGATCACC

Downstream 100 bases:

>100_bases
AAATAAATGGAATATGAACTTTCAGGGCTTTCCAGCCACTGATACCCTTATCCTTCTGTTTCTGGGTCCGTCGAGTTCTG
CGAAGAAGATGCTCTGCCAG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MPVITFDYDDLKELGIDIDREKLLDVLPMMGSDIEDFDDESIKVEFFPNRPDLLSVEGVARSLRGFLGIEKGMPSYDVHD
SGVEVTVDESVLDVRPYLGMAVIEDVQFTDKKLKQVMEFQEDLHWVIGRDRRKVAIGIHDLDRVEPPFLYSGVEPEGVTF
TPLDSVCEMTPHEILEEHPKGVSYAHLLRDHDRYPLITDKNGDVLSMPPIINGELTKLTVNTRRILVDVTGTDDRAVRQT
LNIICTSFAEAGGRIGSVRVKRPDGELHLPDLTPREMRVSVSEASRITGLELSADEVMDLLMKARMDARRTSDDEVVAVI
PAYRVDILHEVDLVENIAVQHCIGRIEPELPDIATIAEEDTWSRADASIREVMVGLGFQEVMSLMLTSEESHYRRMRLEE
DERVEVAQPISQDRTMIRKSLLNGLLEFFEDNKHEDLPQKIFEVGDVVYINPESETRSRVVTKLACAVTHSSAGFTEIKS
LAAAVVENLGYEFRIEPLEHPSFIEGRCAAIESEGKSSAIGGFFGEVHPEVVTNFNLEYPVIALEIEFK

Sequences:

>Translated_549_residues
MPVITFDYDDLKELGIDIDREKLLDVLPMMGSDIEDFDDESIKVEFFPNRPDLLSVEGVARSLRGFLGIEKGMPSYDVHD
SGVEVTVDESVLDVRPYLGMAVIEDVQFTDKKLKQVMEFQEDLHWVIGRDRRKVAIGIHDLDRVEPPFLYSGVEPEGVTF
TPLDSVCEMTPHEILEEHPKGVSYAHLLRDHDRYPLITDKNGDVLSMPPIINGELTKLTVNTRRILVDVTGTDDRAVRQT
LNIICTSFAEAGGRIGSVRVKRPDGELHLPDLTPREMRVSVSEASRITGLELSADEVMDLLMKARMDARRTSDDEVVAVI
PAYRVDILHEVDLVENIAVQHCIGRIEPELPDIATIAEEDTWSRADASIREVMVGLGFQEVMSLMLTSEESHYRRMRLEE
DERVEVAQPISQDRTMIRKSLLNGLLEFFEDNKHEDLPQKIFEVGDVVYINPESETRSRVVTKLACAVTHSSAGFTEIKS
LAAAVVENLGYEFRIEPLEHPSFIEGRCAAIESEGKSSAIGGFFGEVHPEVVTNFNLEYPVIALEIEFK
>Mature_548_residues
PVITFDYDDLKELGIDIDREKLLDVLPMMGSDIEDFDDESIKVEFFPNRPDLLSVEGVARSLRGFLGIEKGMPSYDVHDS
GVEVTVDESVLDVRPYLGMAVIEDVQFTDKKLKQVMEFQEDLHWVIGRDRRKVAIGIHDLDRVEPPFLYSGVEPEGVTFT
PLDSVCEMTPHEILEEHPKGVSYAHLLRDHDRYPLITDKNGDVLSMPPIINGELTKLTVNTRRILVDVTGTDDRAVRQTL
NIICTSFAEAGGRIGSVRVKRPDGELHLPDLTPREMRVSVSEASRITGLELSADEVMDLLMKARMDARRTSDDEVVAVIP
AYRVDILHEVDLVENIAVQHCIGRIEPELPDIATIAEEDTWSRADASIREVMVGLGFQEVMSLMLTSEESHYRRMRLEED
ERVEVAQPISQDRTMIRKSLLNGLLEFFEDNKHEDLPQKIFEVGDVVYINPESETRSRVVTKLACAVTHSSAGFTEIKSL
AAAVVENLGYEFRIEPLEHPSFIEGRCAAIESEGKSSAIGGFFGEVHPEVVTNFNLEYPVIALEIEFK

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B5 domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=529, Percent_Identity=31.5689981096408, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI24308207, Length=165, Percent_Identity=30.3030303030303, Blast_Score=72, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17533403, Length=533, Percent_Identity=32.2701688555347, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17510303, Length=201, Percent_Identity=26.3681592039801, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323089, Length=539, Percent_Identity=29.1280148423006, Blast_Score=233, Evalue=7e-62,
Organism=Drosophila melanogaster, GI21355211, Length=517, Percent_Identity=30.9477756286267, Blast_Score=252, Evalue=4e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_METTH (O26864)

Other databases:

- EMBL:   AE000666
- PIR:   A69203
- RefSeq:   NP_275912.2
- ProteinModelPortal:   O26864
- SMR:   O26864
- IntAct:   O26864
- STRING:   O26864
- GeneID:   1471178
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH770
- NMPDR:   fig|187420.1.peg.767
- eggNOG:   arNOG04353
- HOGENOM:   HBG497667
- OMA:   LLTRMCL
- PhylomeDB:   O26864
- ProtClustDB:   PRK09616
- BioCyc:   MTHE187420:MTH770-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00284
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR004531
- InterPro:   IPR022918
- Gene3D:   G3DSA:3.30.56.20
- SMART:   SM00873
- SMART:   SM00874
- TIGRFAMs:   TIGR00471

Pfam domain/function: PF03483 B3_4; PF03484 B5; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 61805; Mature: 61674

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS51483 B5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVITFDYDDLKELGIDIDREKLLDVLPMMGSDIEDFDDESIKVEFFPNRPDLLSVEGVA
CCEEECCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCEEEHHHHH
RSLRGFLGIEKGMPSYDVHDSGVEVTVDESVLDVRPYLGMAVIEDVQFTDKKLKQVMEFQ
HHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
EDLHWVIGRDRRKVAIGIHDLDRVEPPFLYSGVEPEGVTFTPLDSVCEMTPHEILEEHPK
HHHHHHHCCCCCEEEEEEHHHHCCCCCCEECCCCCCCCEECCHHHHHCCCHHHHHHHCCC
GVSYAHLLRDHDRYPLITDKNGDVLSMPPIINGELTKLTVNTRRILVDVTGTDDRAVRQT
CCHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCEEEEEEECEEEEEEEECCCHHHHHHH
LNIICTSFAEAGGRIGSVRVKRPDGELHLPDLTPREMRVSVSEASRITGLELSADEVMDL
HHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCHHHHEEEHHHHCCCCCCCCCHHHHHHH
LMKARMDARRTSDDEVVAVIPAYRVDILHEVDLVENIAVQHCIGRIEPELPDIATIAEED
HHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
TWSRADASIREVMVGLGFQEVMSLMLTSEESHYRRMRLEEDERVEVAQPISQDRTMIRKS
CCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHH
LLNGLLEFFEDNKHEDLPQKIFEVGDVVYINPESETRSRVVTKLACAVTHSSAGFTEIKS
HHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
LAAAVVENLGYEFRIEPLEHPSFIEGRCAAIESEGKSSAIGGFFGEVHPEVVTNFNLEYP
HHHHHHHHCCCEEEECCCCCCCCCCCHHHEECCCCCCCCCCCHHHHCCHHHHHCCCCCCC
VIALEIEFK
EEEEEEECC
>Mature Secondary Structure 
PVITFDYDDLKELGIDIDREKLLDVLPMMGSDIEDFDDESIKVEFFPNRPDLLSVEGVA
CEEECCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCEEEHHHHH
RSLRGFLGIEKGMPSYDVHDSGVEVTVDESVLDVRPYLGMAVIEDVQFTDKKLKQVMEFQ
HHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
EDLHWVIGRDRRKVAIGIHDLDRVEPPFLYSGVEPEGVTFTPLDSVCEMTPHEILEEHPK
HHHHHHHCCCCCEEEEEEHHHHCCCCCCEECCCCCCCCEECCHHHHHCCCHHHHHHHCCC
GVSYAHLLRDHDRYPLITDKNGDVLSMPPIINGELTKLTVNTRRILVDVTGTDDRAVRQT
CCHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCEEEEEEECEEEEEEEECCCHHHHHHH
LNIICTSFAEAGGRIGSVRVKRPDGELHLPDLTPREMRVSVSEASRITGLELSADEVMDL
HHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCHHHHEEEHHHHCCCCCCCCCHHHHHHH
LMKARMDARRTSDDEVVAVIPAYRVDILHEVDLVENIAVQHCIGRIEPELPDIATIAEED
HHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
TWSRADASIREVMVGLGFQEVMSLMLTSEESHYRRMRLEEDERVEVAQPISQDRTMIRKS
CCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHH
LLNGLLEFFEDNKHEDLPQKIFEVGDVVYINPESETRSRVVTKLACAVTHSSAGFTEIKS
HHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
LAAAVVENLGYEFRIEPLEHPSFIEGRCAAIESEGKSSAIGGFFGEVHPEVVTNFNLEYP
HHHHHHHHCCCEEEECCCCCCCCCCCHHHEECCCCCCCCCCCHHHHCCHHHHHCCCCCCC
VIALEIEFK
EEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 10575359