Click here to switch to the map view.

The map label for this gene is aroF [H]

Identifier: 161377474

GI number: 161377474

Start: 2110218

End: 2111291

Strand: Reverse

Name: aroF [H]

Synonym: Daro_1964

Alternate gene names: 161377474

Gene position: 2111291-2110218 (Counterclockwise)

Preceding gene: 71907594

Following gene: 71907592

Centisome position: 46.91

GC content: 61.45

Gene sequence:

>1074_bases
ATGACGACACAGAACATCGACAACGTAAACGTAACCGCCTTCGACGCCATGCCGACGCCGGAAGAAATCAACGCCCGCCA
GCCGCTCAGCGCCAAGGCCGCCAAAACGGTTACCCACGGCCGCAACCTGCTGCGCAAGATTCTCGACCGCAAGGATCACC
GCCTGTTCGTCGTTGTCGGCCCCTGCTCCATCCACGACCCGGTCGCCGGCCTGGATTACGCCCGCCGCCTGAAGAAACTG
GCCGATGAAGTCGGTGACGTGCTGCAGATCATCATGCGTGTCTATTTCGAAAAACCGCGCACCACAGTGGGCTGGAAAGG
TTATATCAACGACCCGTTCATGGACGACTCCTTCCAGGTCAATGTCGGCATGGAAAAGGCGCGTGAATTCCTGCTCCAGG
TCAATGAGCTCGGCCTGCCCGCCGGTACCGAAGCCCTCGACCCCTACGGCCCGCAATACTACGGCGACCTGATCACCTGG
ACCGCCATCGGCGCCCGCACCACCGAATCGCAGACCCACCGCGAAATGTCTTCCGGCCTGTCGACCCCGGTCGGCTTCAA
GAACGCCACCAATGGCGACCTGTCAGTGGCCGTCAATGCCATCCTCTCCGCCTCGCGTCCGCACTCCTTCCTTGGTCTGA
ACAGCGAAGGCCGTGTCGCCATCGTCCGCACCAAGGGCAACGGCTACGGCCACGTCGTGCTGCGTGGTGGTGACGGTCGT
CCGAATTACGACACGGTCTCCGTCTCCATCGCCGAACAGGCCATGGTCAAGGCCAAGCTGCCGGCCAACATCGTCGTCGA
TTGCTCACACGCCAACAGCTCCAAGAAACCTGAATTGCAGCCGCTGGTCATGGCCGATGTCGTCAACCAGATCCGCCTCG
GCAACAAATCACTGCTTGGCGTGATGATCGAGTCGAACATCGAAGCCGGCAACCAGTCCATCCCGGCTGACCTCAGCCAG
CTCAAGTACGGCTGCTCGGTCACCGATGGCTGTGTCGGTTGGGACACCACCGAAAAGATGATCCGCGACGCCGCCGTGTT
GTTGCGCGACGTGCTGCCGGAACGTCTGTCCTGA

Upstream 100 bases:

>100_bases
TTAGCCGGACAGGATAAAATTTCCCTTTTGCGCCACTGCCCGACCTTGAACGACAAGGTTGCCGCAGTCAAAGACATGAT
TCGCCAGCTGAGCCACTGAC

Downstream 100 bases:

>100_bases
CCGAATCGACAGACGGAACATTGCGAACAAGCCAGTGAATCACAAGAATGGCACCCGCTTCACGCTCATTGCCGAAAGCA
TTGGCCAATTGCTGGATACC

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTTQNIDNVNVTAFDAMPTPEEINARQPLSAKAAKTVTHGRNLLRKILDRKDHRLFVVVGPCSIHDPVAGLDYARRLKKL
ADEVGDVLQIIMRVYFEKPRTTVGWKGYINDPFMDDSFQVNVGMEKAREFLLQVNELGLPAGTEALDPYGPQYYGDLITW
TAIGARTTESQTHREMSSGLSTPVGFKNATNGDLSVAVNAILSASRPHSFLGLNSEGRVAIVRTKGNGYGHVVLRGGDGR
PNYDTVSVSIAEQAMVKAKLPANIVVDCSHANSSKKPELQPLVMADVVNQIRLGNKSLLGVMIESNIEAGNQSIPADLSQ
LKYGCSVTDGCVGWDTTEKMIRDAAVLLRDVLPERLS

Sequences:

>Translated_357_residues
MTTQNIDNVNVTAFDAMPTPEEINARQPLSAKAAKTVTHGRNLLRKILDRKDHRLFVVVGPCSIHDPVAGLDYARRLKKL
ADEVGDVLQIIMRVYFEKPRTTVGWKGYINDPFMDDSFQVNVGMEKAREFLLQVNELGLPAGTEALDPYGPQYYGDLITW
TAIGARTTESQTHREMSSGLSTPVGFKNATNGDLSVAVNAILSASRPHSFLGLNSEGRVAIVRTKGNGYGHVVLRGGDGR
PNYDTVSVSIAEQAMVKAKLPANIVVDCSHANSSKKPELQPLVMADVVNQIRLGNKSLLGVMIESNIEAGNQSIPADLSQ
LKYGCSVTDGCVGWDTTEKMIRDAAVLLRDVLPERLS
>Mature_356_residues
TTQNIDNVNVTAFDAMPTPEEINARQPLSAKAAKTVTHGRNLLRKILDRKDHRLFVVVGPCSIHDPVAGLDYARRLKKLA
DEVGDVLQIIMRVYFEKPRTTVGWKGYINDPFMDDSFQVNVGMEKAREFLLQVNELGLPAGTEALDPYGPQYYGDLITWT
AIGARTTESQTHREMSSGLSTPVGFKNATNGDLSVAVNAILSASRPHSFLGLNSEGRVAIVRTKGNGYGHVVLRGGDGRP
NYDTVSVSIAEQAMVKAKLPANIVVDCSHANSSKKPELQPLVMADVVNQIRLGNKSLLGVMIESNIEAGNQSIPADLSQL
KYGCSVTDGCVGWDTTEKMIRDAAVLLRDVLPERLS

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=343, Percent_Identity=53.3527696793003, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1786969, Length=345, Percent_Identity=51.5942028985507, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI1787996, Length=339, Percent_Identity=46.3126843657817, Blast_Score=320, Evalue=8e-89,
Organism=Saccharomyces cerevisiae, GI6319726, Length=347, Percent_Identity=48.4149855907781, Blast_Score=328, Evalue=6e-91,
Organism=Saccharomyces cerevisiae, GI6320240, Length=344, Percent_Identity=45.6395348837209, Blast_Score=313, Evalue=2e-86,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 38888; Mature: 38757

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQNIDNVNVTAFDAMPTPEEINARQPLSAKAAKTVTHGRNLLRKILDRKDHRLFVVVG
CCCCCCCCCEEEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PCSIHDPVAGLDYARRLKKLADEVGDVLQIIMRVYFEKPRTTVGWKGYINDPFMDDSFQV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
NVGMEKAREFLLQVNELGLPAGTEALDPYGPQYYGDLITWTAIGARTTESQTHREMSSGL
ECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHEEEECCCCCCHHHHHHHHHCCC
STPVGFKNATNGDLSVAVNAILSASRPHSFLGLNSEGRVAIVRTKGNGYGHVVLRGGDGR
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCEEEEEEECCCCC
PNYDTVSVSIAEQAMVKAKLPANIVVDCSHANSSKKPELQPLVMADVVNQIRLGNKSLLG
CCCCEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
VMIESNIEAGNQSIPADLSQLKYGCSVTDGCVGWDTTEKMIRDAAVLLRDVLPERLS
EEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQNIDNVNVTAFDAMPTPEEINARQPLSAKAAKTVTHGRNLLRKILDRKDHRLFVVVG
CCCCCCCCEEEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PCSIHDPVAGLDYARRLKKLADEVGDVLQIIMRVYFEKPRTTVGWKGYINDPFMDDSFQV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
NVGMEKAREFLLQVNELGLPAGTEALDPYGPQYYGDLITWTAIGARTTESQTHREMSSGL
ECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHEEEECCCCCCHHHHHHHHHCCC
STPVGFKNATNGDLSVAVNAILSASRPHSFLGLNSEGRVAIVRTKGNGYGHVVLRGGDGR
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCEEEEEEECCCCC
PNYDTVSVSIAEQAMVKAKLPANIVVDCSHANSSKKPELQPLVMADVVNQIRLGNKSLLG
CCCCEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
VMIESNIEAGNQSIPADLSQLKYGCSVTDGCVGWDTTEKMIRDAAVLLRDVLPERLS
EEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]