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The map label for this gene is ilvD [H]

Identifier: 161353769

GI number: 161353769

Start: 2459159

End: 2460865

Strand: Direct

Name: ilvD [H]

Synonym: BRADO2308

Alternate gene names: 161353769

Gene position: 2459159-2460865 (Clockwise)

Preceding gene: 146339328

Following gene: 146339330

Centisome position: 32.98

GC content: 68.19

Gene sequence:

>1707_bases
ATGAGGAAGCTGCGTTCGCGCGTGACGGTGGAGGGGCTCGACCGCGCCCCGCACCGCGCCTTCATGCGCGCGATGGGCCT
CGATGACGCCGACATCGCCAAGCCGATGATCGGAATCGTCAGTCAGAGGGGCGAGCAGACGCCCTGCAACATGACGCACG
ACTTCCAGGTCGATGCCGCCAAGACCGGCATCTCCGAGTCCGGCGGCACGCCGCGCGAATTCGCCACCGTCTCCGTCTCC
GACGGCATCAGCATGAACCATGAGGGAATGAAGTTCTCTCTGTTCTCCCGCGAGCTGATCGCCGATTCGATCGAGGCCGT
GGTGCACGGCCTAGCCTATGACGCGCTGATCGGCTTCGGCGGCTGCGACAAGACCTTGCCCGGCGTGATGATGGGTATGG
TCCGCTGCAACGTGCCGTCGATCTTCATCTATGGCGGCAGCGCGCTGCCCGGCAAGTACCAGGGCAAGACGCTCACGGTG
CTCGACTCCTACGAAGCGGTCGGCAGCTACATGACCGGCGAGATCGATGCCGCCACGCTCGAAGGCATCGAGCGCTCGTG
CCTGCCGACGATCGGCGCCTGCGCCGGCCAGTTCACCGCGAACACCATGGGCATGCTGTCTGAGGCGATGGGCCTGTCGA
TGCCCAACGTCTCGATGATCCCCGGCGTCTATGCCGAGCGCGCCCATGTCGCGCGCGCAGCCGGGCGCCTGATCATGCAG
ATGCTCGCCAACGATGGCCCGCGGCCGCGCGACATCGTCACCCGCAAATCCCTGGAGAACGGCGCGGCGATCGTCGCCGC
CACCGGCGGCTCGACCAACGCGGCCCTGCATCTGCCTGCGATCGCGAACGAGGCCGGCATCCGCTTCACGCTCGACGATG
TCGGCGAGGTGTTTTCGCGCACGCCGCTGATCGGCAATCTCAGGCCCGGCGGCAAGTATACGGCGAAGGACGTGTATGAC
ATCGGCGGCGCGGCCGTCGTGATCCGCGCGCTGATCGACAGCGGCCACATCGACGGCTCCTGCATCACGGTGACCGGCCG
TACGCTCGCCGAGGCCTGCGGCGCGGCCAATGCGCCGGATGGCGAGGTCGTGTTCACGCCGGCCAGGCCGATGATGGCCG
ACGGCGGCGTCGCCGTGCTCAAGGGCAATCTCGCGCCGGATGGTGCGGTCATCAAGGTCGCCGGTCTCAAGAGCCTGGTG
TTCGAGGGCCGCGCGCGCGTGTTTGAAGATGAAGAAGCCTGCGTCGCCGCCGTCCGCGCCCGCAACTACCAGGCGGGCGA
GGTGCTGATCATCCGTAACGAGGGGCCGGTCGGCGGCCCCGGCATGCGCGAGATGCTCGGCGTCACCGCGCTGATCTATG
GCCAGGGCATGGGCGAGAAGGTCGCGCTGATCACCGACGGCCGCTTCTCCGGTGCCACCCGCGGTTTATGCATCGGCTAC
GTCTCGCCGGAAGCCTTCGTCGGCGGCCCGCTGGCGCTGGTGCGTGATGGCGATCCCATTCGCATCGATGCGAAAGCGCG
CACGCTCGACGTGCTGCTCGACGAGGGCGAGCTCGCCGCGCGTCGCAGCGCGTGGACGCAGCGGCCGCCGCGGCATCGCG
CGGGAGCGCTGGCGAAATACGCGAAACTGGTGGGGCAGGCGCCGCTCGGCGCGGTCACGCATGAGGGACCGGCGGAATGG
CCCTGGTTCGATCAGGAGCAGGAATGA

Upstream 100 bases:

>100_bases
GCCGAGACCGGCATGCTGCCGAAGCTCGACGTCGCCCAGTTCAAGCACCCCACCATCGTCGCGCCGATCCAGTAGGCGTC
GCGACGCGGAGCTCGCATCG

Downstream 100 bases:

>100_bases
ACCGCGACGCGCGACGATTGGCAGGGTTTTTGCTAATCTTGCGACCACACGCCCCGTCATTCCAGGGCAATCGTCGAGGC
GAAGCCTCGATGATTGAGCC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 3 [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MRKLRSRVTVEGLDRAPHRAFMRAMGLDDADIAKPMIGIVSQRGEQTPCNMTHDFQVDAAKTGISESGGTPREFATVSVS
DGISMNHEGMKFSLFSRELIADSIEAVVHGLAYDALIGFGGCDKTLPGVMMGMVRCNVPSIFIYGGSALPGKYQGKTLTV
LDSYEAVGSYMTGEIDAATLEGIERSCLPTIGACAGQFTANTMGMLSEAMGLSMPNVSMIPGVYAERAHVARAAGRLIMQ
MLANDGPRPRDIVTRKSLENGAAIVAATGGSTNAALHLPAIANEAGIRFTLDDVGEVFSRTPLIGNLRPGGKYTAKDVYD
IGGAAVVIRALIDSGHIDGSCITVTGRTLAEACGAANAPDGEVVFTPARPMMADGGVAVLKGNLAPDGAVIKVAGLKSLV
FEGRARVFEDEEACVAAVRARNYQAGEVLIIRNEGPVGGPGMREMLGVTALIYGQGMGEKVALITDGRFSGATRGLCIGY
VSPEAFVGGPLALVRDGDPIRIDAKARTLDVLLDEGELAARRSAWTQRPPRHRAGALAKYAKLVGQAPLGAVTHEGPAEW
PWFDQEQE

Sequences:

>Translated_568_residues
MRKLRSRVTVEGLDRAPHRAFMRAMGLDDADIAKPMIGIVSQRGEQTPCNMTHDFQVDAAKTGISESGGTPREFATVSVS
DGISMNHEGMKFSLFSRELIADSIEAVVHGLAYDALIGFGGCDKTLPGVMMGMVRCNVPSIFIYGGSALPGKYQGKTLTV
LDSYEAVGSYMTGEIDAATLEGIERSCLPTIGACAGQFTANTMGMLSEAMGLSMPNVSMIPGVYAERAHVARAAGRLIMQ
MLANDGPRPRDIVTRKSLENGAAIVAATGGSTNAALHLPAIANEAGIRFTLDDVGEVFSRTPLIGNLRPGGKYTAKDVYD
IGGAAVVIRALIDSGHIDGSCITVTGRTLAEACGAANAPDGEVVFTPARPMMADGGVAVLKGNLAPDGAVIKVAGLKSLV
FEGRARVFEDEEACVAAVRARNYQAGEVLIIRNEGPVGGPGMREMLGVTALIYGQGMGEKVALITDGRFSGATRGLCIGY
VSPEAFVGGPLALVRDGDPIRIDAKARTLDVLLDEGELAARRSAWTQRPPRHRAGALAKYAKLVGQAPLGAVTHEGPAEW
PWFDQEQE
>Mature_568_residues
MRKLRSRVTVEGLDRAPHRAFMRAMGLDDADIAKPMIGIVSQRGEQTPCNMTHDFQVDAAKTGISESGGTPREFATVSVS
DGISMNHEGMKFSLFSRELIADSIEAVVHGLAYDALIGFGGCDKTLPGVMMGMVRCNVPSIFIYGGSALPGKYQGKTLTV
LDSYEAVGSYMTGEIDAATLEGIERSCLPTIGACAGQFTANTMGMLSEAMGLSMPNVSMIPGVYAERAHVARAAGRLIMQ
MLANDGPRPRDIVTRKSLENGAAIVAATGGSTNAALHLPAIANEAGIRFTLDDVGEVFSRTPLIGNLRPGGKYTAKDVYD
IGGAAVVIRALIDSGHIDGSCITVTGRTLAEACGAANAPDGEVVFTPARPMMADGGVAVLKGNLAPDGAVIKVAGLKSLV
FEGRARVFEDEEACVAAVRARNYQAGEVLIIRNEGPVGGPGMREMLGVTALIYGQGMGEKVALITDGRFSGATRGLCIGY
VSPEAFVGGPLALVRDGDPIRIDAKARTLDVLLDEGELAARRSAWTQRPPRHRAGALAKYAKLVGQAPLGAVTHEGPAEW
PWFDQEQE

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=608, Percent_Identity=35.8552631578947, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1788157, Length=481, Percent_Identity=31.1850311850312, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI2367371, Length=495, Percent_Identity=30.7070707070707, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786464, Length=493, Percent_Identity=32.2515212981744, Blast_Score=184, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6322476, Length=557, Percent_Identity=41.4721723518851, Blast_Score=427, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59706; Mature: 59706

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLRSRVTVEGLDRAPHRAFMRAMGLDDADIAKPMIGIVSQRGEQTPCNMTHDFQVDAA
CCCHHHCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHH
KTGISESGGTPREFATVSVSDGISMNHEGMKFSLFSRELIADSIEAVVHGLAYDALIGFG
HCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
GCDKTLPGVMMGMVRCNVPSIFIYGGSALPGKYQGKTLTVLDSYEAVGSYMTGEIDAATL
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECHHHHCCHHCCCCCHHHH
EGIERSCLPTIGACAGQFTANTMGMLSEAMGLSMPNVSMIPGVYAERAHVARAAGRLIMQ
HHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
MLANDGPRPRDIVTRKSLENGAAIVAATGGSTNAALHLPAIANEAGIRFTLDDVGEVFSR
HHHCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCEEECCEECCCCCCEEEHHHHHHHHHC
TPLIGNLRPGGKYTAKDVYDIGGAAVVIRALIDSGHIDGSCITVTGRTLAEACGAANAPD
CCCEECCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHCCCCCCC
GEVVFTPARPMMADGGVAVLKGNLAPDGAVIKVAGLKSLVFEGRARVFEDEEACVAAVRA
CCEEECCCCCCCCCCCEEEEECCCCCCCCEEEECCHHHHHHHCCHHEECCHHHHHHHHHH
RNYQAGEVLIIRNEGPVGGPGMREMLGVTALIYGQGMGEKVALITDGRFSGATRGLCIGY
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEECCCCCCEEEEEECCCCCCCCCCEEEEE
VSPEAFVGGPLALVRDGDPIRIDAKARTLDVLLDEGELAARRSAWTQRPPRHRAGALAKY
ECCCHHCCCCEEEEECCCCEEEECCCEEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHH
AKLVGQAPLGAVTHEGPAEWPWFDQEQE
HHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKLRSRVTVEGLDRAPHRAFMRAMGLDDADIAKPMIGIVSQRGEQTPCNMTHDFQVDAA
CCCHHHCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHH
KTGISESGGTPREFATVSVSDGISMNHEGMKFSLFSRELIADSIEAVVHGLAYDALIGFG
HCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
GCDKTLPGVMMGMVRCNVPSIFIYGGSALPGKYQGKTLTVLDSYEAVGSYMTGEIDAATL
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECHHHHCCHHCCCCCHHHH
EGIERSCLPTIGACAGQFTANTMGMLSEAMGLSMPNVSMIPGVYAERAHVARAAGRLIMQ
HHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
MLANDGPRPRDIVTRKSLENGAAIVAATGGSTNAALHLPAIANEAGIRFTLDDVGEVFSR
HHHCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCEEECCEECCCCCCEEEHHHHHHHHHC
TPLIGNLRPGGKYTAKDVYDIGGAAVVIRALIDSGHIDGSCITVTGRTLAEACGAANAPD
CCCEECCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHCCCCCCC
GEVVFTPARPMMADGGVAVLKGNLAPDGAVIKVAGLKSLVFEGRARVFEDEEACVAAVRA
CCEEECCCCCCCCCCCEEEEECCCCCCCCEEEECCHHHHHHHCCHHEECCHHHHHHHHHH
RNYQAGEVLIIRNEGPVGGPGMREMLGVTALIYGQGMGEKVALITDGRFSGATRGLCIGY
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEECCCCCCEEEEEECCCCCCCCCCEEEEE
VSPEAFVGGPLALVRDGDPIRIDAKARTLDVLLDEGELAARRSAWTQRPPRHRAGALAKY
ECCCHHCCCCEEEEECCCCEEEECCCEEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHH
AKLVGQAPLGAVTHEGPAEWPWFDQEQE
HHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]