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The map label for this gene is proB [H]

Identifier: 161353745

GI number: 161353745

Start: 3754820

End: 3755929

Strand: Direct

Name: proB [H]

Synonym: alr3103

Alternate gene names: 161353745

Gene position: 3754820-3755929 (Clockwise)

Preceding gene: 17230594

Following gene: 17230597

Centisome position: 58.54

GC content: 46.4

Gene sequence:

>1110_bases
TTGACCAAAACAATTGTCGTCAAAATTGGCACTTCTAGCCTTACCCAACCAGAAACCGGACAACTAGCCCTCTCTACCAT
TGCAACTTTGGCGGAAACACTCTCTCATTTAAGACAGCAAGGTAATCGGGTGATTTTAGTTTCCTCTGGGGCTGTGGGAG
TGGGTTGTGCGCGGTTGGGTTTAACCGAACGTCCCAAAGCGATCGCCCTCAAACAGGCGGTGGCCGCAGTGGGTCAAGGT
CGATTAATGCGCGTATATGACGACTTATTTACCACCTTACAACAGCCAATTGCCCAAGTGTTGCTAACTCGCAGCGATTT
GGTACAACGTAGCCGTTATCTCAACGTATACAACACCTTTCGGGAATTACTCGCGTTGGGGGTAATTCCTGTAGTCAACG
AGAATGATACCGTTGCCGTCGATGAACTGAAATTTGGTGATAATGATACCCTTTCGGCTTTGGTTGCTAGTCTTGTAGAG
GCGGACTGGCTATTTTTACTCACAGACGTAGACCGATTATACTCAGCCGATCCCCGTTCTGTCCCCGATGCCCGACCAAT
ATCTTTGGTGACAAGCATCAAAGAATTAGCAGACTTACAAGTGCAAACAGGTTCTCAAGGTTCTCAATGGGGTACTGGGG
GAATGATGACAAAAATTTCGGCGGCGAGAATTGCGATCGCGGCGGGAGTGCGTACTGTGATTACTCAAGGACGATACCCC
CGTAATATCGAGAAAATTATCCAAGGGGAACTGATTGGAACTCATTTTCAACCCCAACCAGAACCAACTTCCGCCCGTAA
ACGTTGGATTGCTTACGGTTTAGTCCCGGTTGGTAAATTATACTTAGATTCTGGTGCGATCGCCGCTATAGTTAAAGCAG
GTAAATCCTTATTGCCAGCAGGAGTTAAAACCGTTGCAGGTGAATTTGAGCCTCAAGATGCAGTACAGTTATGCGATCCT
CAAGGTAACGAAATTGCCAGAGGACTGGTTAACTATAGCAGCAATGATTTACAGAAAATTTGTGGTCGTCATTCTAAGGA
GATTCCGACGATTTTGGGTTATGTAGGTGCGGAAACCGTGATCCATCGAGATAACTTAGTATTGACTTAA

Upstream 100 bases:

>100_bases
GGTTCTCCGCAGGAGTACAATTAGTGGCGAGTCCAAGCTCCCACTAATTGTCTTTAATTTTGAATTTTGAATTTTGAATT
TTGAATTGGAGCGAAGCGAC

Downstream 100 bases:

>100_bases
AAAATAAAAACCCCGTTAAATAGCGGGGTTAATGTTACTTTAGCCACAAAATTTACCATAAAAAATTACTAGCTAGCACC
CTGAAAATAGGTAAAAGGTA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTKTIVVKIGTSSLTQPETGQLALSTIATLAETLSHLRQQGNRVILVSSGAVGVGCARLGLTERPKAIALKQAVAAVGQG
RLMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTFRELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVE
ADWLFLLTDVDRLYSADPRSVPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYP
RNIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPVGKLYLDSGAIAAIVKAGKSLLPAGVKTVAGEFEPQDAVQLCDP
QGNEIARGLVNYSSNDLQKICGRHSKEIPTILGYVGAETVIHRDNLVLT

Sequences:

>Translated_369_residues
MTKTIVVKIGTSSLTQPETGQLALSTIATLAETLSHLRQQGNRVILVSSGAVGVGCARLGLTERPKAIALKQAVAAVGQG
RLMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTFRELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVE
ADWLFLLTDVDRLYSADPRSVPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYP
RNIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPVGKLYLDSGAIAAIVKAGKSLLPAGVKTVAGEFEPQDAVQLCDP
QGNEIARGLVNYSSNDLQKICGRHSKEIPTILGYVGAETVIHRDNLVLT
>Mature_368_residues
TKTIVVKIGTSSLTQPETGQLALSTIATLAETLSHLRQQGNRVILVSSGAVGVGCARLGLTERPKAIALKQAVAAVGQGR
LMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTFRELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVEA
DWLFLLTDVDRLYSADPRSVPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYPR
NIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPVGKLYLDSGAIAAIVKAGKSLLPAGVKTVAGEFEPQDAVQLCDPQ
GNEIARGLVNYSSNDLQKICGRHSKEIPTILGYVGAETVIHRDNLVLT

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=288, Percent_Identity=32.6388888888889, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=32.5174825174825, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.9890710382514, Blast_Score=297, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=35.5805243445693, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=35.5805243445693, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=41.1057692307692, Blast_Score=285, Evalue=8e-78,
Organism=Saccharomyces cerevisiae, GI6321822, Length=422, Percent_Identity=39.0995260663507, Blast_Score=264, Evalue=1e-71,
Organism=Drosophila melanogaster, GI21357643, Length=294, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39690; Mature: 39559

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTIVVKIGTSSLTQPETGQLALSTIATLAETLSHLRQQGNRVILVSSGAVGVGCARLG
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCC
LTERPKAIALKQAVAAVGQGRLMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTF
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVEADWLFLLTDVDRLYSADPRS
HHHHHHCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYP
CCCCCHHHHHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RNIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPVGKLYLDSGAIAAIVKAGKSLLPA
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHEECCCHHHHHHHHCHHHCCC
GVKTVAGEFEPQDAVQLCDPQGNEIARGLVNYSSNDLQKICGRHSKEIPTILGYVGAETV
HHHHHCCCCCCCHHHHHCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHCHHHE
IHRDNLVLT
EECCCEEEC
>Mature Secondary Structure 
TKTIVVKIGTSSLTQPETGQLALSTIATLAETLSHLRQQGNRVILVSSGAVGVGCARLG
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCC
LTERPKAIALKQAVAAVGQGRLMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTF
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVEADWLFLLTDVDRLYSADPRS
HHHHHHCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYP
CCCCCHHHHHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RNIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPVGKLYLDSGAIAAIVKAGKSLLPA
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHEECCCHHHHHHHHCHHHCCC
GVKTVAGEFEPQDAVQLCDPQGNEIARGLVNYSSNDLQKICGRHSKEIPTILGYVGAETV
HHHHHCCCCCCCHHHHHCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHCHHHE
IHRDNLVLT
EECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]