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The map label for this gene is genX [H]

Identifier: 161350024

GI number: 161350024

Start: 885898

End: 886869

Strand: Direct

Name: genX [H]

Synonym: HI0836

Alternate gene names: 161350024

Gene position: 885898-886869 (Clockwise)

Preceding gene: 30995405

Following gene: 16272781

Centisome position: 48.41

GC content: 39.2

Gene sequence:

>972_bases
ATGACCGCACTTAATCATTGGCAACCTTCTGCTGATATAAAAAATCATCTTAAACGGGCAAAAATTATTGCTAAAATTCG
TCAATTTTTTACAGAACGCGGTTTGCTTGAAGTAGAGACCCCTGTTCTAAGTGAATTTGGCGTCACTGATTTACATCTTT
CCACCTTTAGCACGGAATTTCTCGCGCCTTTCGGTGAACAATCCAAAACACTTTGGCTTTCTACAAGCCCTGAATATCAT
ATGAAACGCTTGCTTGCTGCAGGCAGTGGGCCGATATTCCAAATCAGTAAAGTGTTTCGCAACGAAGAGGCTGGAAATCG
CCATAATCCAGAATTCACTATGTTGGAATGGTATCGACCGCACTTTCATATGCATCGTTTAATTAATGAAGTAGATGATT
TGCTCCAACAAATTTTAGATTGTCCACCTGCAGAAAGTTTAAGCTATCAGTTTGTTTTCCAAGAATATGTAGGCTTAGAT
CCCTTGTCAGCAGAACGTTCAGAATTAATTGAAGCAGCACGTAAACATAATTTTATGGCTGAAGATAATGAAGATCGCGA
TACGTTATTACAGTTTTTATTTAGTGAAGTCGTTGAGCCTCAAATTGGAAAAGAACGACCTATTGCCGTGTATCATTTCC
CTTCAACACAAGCAGCTCTAGCACAAGTAAGCCCAGAAGATCAGCGGGTAGCAGAACGCTTTGAGTTCTATTATAAAGGT
TTAGAATTAGCCAATGGTTTTCACGAACTTGCCGATGCGCAAGAACAACGTCATCGCTTTGAATTAGATAATCAACAAAG
ACAAAAATGTGAATTGCCTACTCGAGAAATTGATGAACGCTTTCTTGCTGCCTTAGAAGCGGGTATGCCAGATGCTTCAG
GTGTCGCATTAGGAATAGATCGTTTAATGATGATCGCTTTAGATTGTGAAAAAATTAATGACGTTATTTCTTTTGCTGTG
GACAATGCTTAA

Upstream 100 bases:

>100_bases
GGAAANGTATCGGATTTTGTGATCTACCTCAAAAAAGCTAAGATTGAGTTGAGAGTAGCTCTCGTTTACAATGTGCAAAA
TTTTTAAAACAGGATTAAAA

Downstream 100 bases:

>100_bases
AGGTAAAAGTGCGGTAAAAATTATCATCATTTTTATTTAATGGGTGATCCATAATGGCAATTTTATATTCATTGATAGCC
CGCCTAATATTTCTTTTTGA

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTALNHWQPSADIKNHLKRAKIIAKIRQFFTERGLLEVETPVLSEFGVTDLHLSTFSTEFLAPFGEQSKTLWLSTSPEYH
MKRLLAAGSGPIFQISKVFRNEEAGNRHNPEFTMLEWYRPHFHMHRLINEVDDLLQQILDCPPAESLSYQFVFQEYVGLD
PLSAERSELIEAARKHNFMAEDNEDRDTLLQFLFSEVVEPQIGKERPIAVYHFPSTQAALAQVSPEDQRVAERFEFYYKG
LELANGFHELADAQEQRHRFELDNQQRQKCELPTREIDERFLAALEAGMPDASGVALGIDRLMMIALDCEKINDVISFAV
DNA

Sequences:

>Translated_323_residues
MTALNHWQPSADIKNHLKRAKIIAKIRQFFTERGLLEVETPVLSEFGVTDLHLSTFSTEFLAPFGEQSKTLWLSTSPEYH
MKRLLAAGSGPIFQISKVFRNEEAGNRHNPEFTMLEWYRPHFHMHRLINEVDDLLQQILDCPPAESLSYQFVFQEYVGLD
PLSAERSELIEAARKHNFMAEDNEDRDTLLQFLFSEVVEPQIGKERPIAVYHFPSTQAALAQVSPEDQRVAERFEFYYKG
LELANGFHELADAQEQRHRFELDNQQRQKCELPTREIDERFLAALEAGMPDASGVALGIDRLMMIALDCEKINDVISFAV
DNA
>Mature_322_residues
TALNHWQPSADIKNHLKRAKIIAKIRQFFTERGLLEVETPVLSEFGVTDLHLSTFSTEFLAPFGEQSKTLWLSTSPEYHM
KRLLAAGSGPIFQISKVFRNEEAGNRHNPEFTMLEWYRPHFHMHRLINEVDDLLQQILDCPPAESLSYQFVFQEYVGLDP
LSAERSELIEAARKHNFMAEDNEDRDTLLQFLFSEVVEPQIGKERPIAVYHFPSTQAALAQVSPEDQRVAERFEFYYKGL
ELANGFHELADAQEQRHRFELDNQQRQKCELPTREIDERFLAALEAGMPDASGVALGIDRLMMIALDCEKINDVISFAVD
NA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=333, Percent_Identity=26.7267267267267, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI5031815, Length=333, Percent_Identity=26.7267267267267, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=62.1538461538461, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1790571, Length=323, Percent_Identity=29.7213622291022, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1789256, Length=329, Percent_Identity=29.1793313069909, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71994340, Length=338, Percent_Identity=28.1065088757396, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17535925, Length=338, Percent_Identity=28.1065088757396, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17535927, Length=338, Percent_Identity=28.1065088757396, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324256, Length=352, Percent_Identity=28.9772727272727, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320242, Length=344, Percent_Identity=25.5813953488372, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640851, Length=333, Percent_Identity=26.7267267267267, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24640849, Length=333, Percent_Identity=26.7267267267267, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 37156; Mature: 37025

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALNHWQPSADIKNHLKRAKIIAKIRQFFTERGLLEVETPVLSEFGVTDLHLSTFSTEF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHCCCCCEEHHHHHHHH
LAPFGEQSKTLWLSTSPEYHMKRLLAAGSGPIFQISKVFRNEEAGNRHNPEFTMLEWYRP
HCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHCH
HFHMHRLINEVDDLLQQILDCPPAESLSYQFVFQEYVGLDPLSAERSELIEAARKHNFMA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
EDNEDRDTLLQFLFSEVVEPQIGKERPIAVYHFPSTQAALAQVSPEDQRVAERFEFYYKG
CCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHH
LELANGFHELADAQEQRHRFELDNQQRQKCELPTREIDERFLAALEAGMPDASGVALGID
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
RLMMIALDCEKINDVISFAVDNA
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TALNHWQPSADIKNHLKRAKIIAKIRQFFTERGLLEVETPVLSEFGVTDLHLSTFSTEF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHCCCCCEEHHHHHHHH
LAPFGEQSKTLWLSTSPEYHMKRLLAAGSGPIFQISKVFRNEEAGNRHNPEFTMLEWYRP
HCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHCH
HFHMHRLINEVDDLLQQILDCPPAESLSYQFVFQEYVGLDPLSAERSELIEAARKHNFMA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
EDNEDRDTLLQFLFSEVVEPQIGKERPIAVYHFPSTQAALAQVSPEDQRVAERFEFYYKG
CCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHH
LELANGFHELADAQEQRHRFELDNQQRQKCELPTREIDERFLAALEAGMPDASGVALGID
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
RLMMIALDCEKINDVISFAVDNA
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA