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The map label for this gene is ptrB [C]

Identifier: 16127918

GI number: 16127918

Start: 3940531

End: 3942645

Strand: Direct

Name: ptrB [C]

Synonym: CC_3688

Alternate gene names: 16127918

Gene position: 3940531-3942645 (Clockwise)

Preceding gene: 16127917

Following gene: 16127919

Centisome position: 98.1

GC content: 66.43

Gene sequence:

>2115_bases
ATGACCCTGACCCCCGCCATCGCCGCCGACACCGCAAAGCCCGCCGGCAAACCCGCCGCCGAGGCCCGCACGCCCCTGTC
GGAGCTGGGCAAGGACGATCCCTATCTGTGGATGGAGGAGATCGAGGGGACGCGCGCGATGGACTGGGCCAAGGCTCAGA
ACGCCCGCTCGCTGCCCGTGCTGCAGGGCGACGCGCGCTACGCCGACCTTGAGTCCAAGGCCCTGGCCATCCTGAACGCC
AAGGACCGCGTGCCCGGCGTGTCGTTCGCCGGCGATGGTTCCCTGCGCAACTTCTGGCAGGACAAGGATCATGTTCGGGG
GATCTGGCGTAAAACGACGCTGGACAGCTACCGCACCGCCGAGCCCGCCTGGGAAACGATCCTCGACATCGACGCCCTGA
CCAAGGCCGAGAACGCCAACTGGGTGTTCAAGGGCGCCAGCTGCCTGCCGCCGGAGGACACCCGCTGCCTCATCACCCTG
TCGAACGGCGGCAAGGACGCGGTGACGGTTCGCGAATTCGACACCACCACCAAGACCTTTGTCCCCGGCGGCTTCGAACT
GCCGGAGGGCAAGCAGAACTACACCTGGCTGGACAAGGACACCCTGCTGGTCGGCCGCGAATGGAAGCCGGGCGAGCTGA
CCAAGTCGGGCTACGCCTATGTCCTGAAAGCGCTCAAGCGCGGGCAGCCCCTGGATCAGGCCAAGGAAGTCTTCCGCGGC
GTCGAGACCGACGTTTCGGTCAGCCCGTTCGTGCTGCGTGACGCCGACGGCAAGGCCGTCGCGGTCATGGCCACGCGTGG
CGTCACCTTCTTCGAGAGCGAGACCTATCTGATCGAGGGCGACAAGCCCGTGAAGCTCGACCTGCCGCTGAAGAGCTCGA
TCCAGGGCTATGTCGACGGCCAGATGGTTGTCCTGATGGAGCAGGACTGGCCGGCGAAGGGCTTCAAGGTCGGCGACCTG
ATCAGCTTCGATCTGGCCAAGCTGAAAGCGGCCCCGGACAAGGCTGTGGCCACGCTGGTGCTGCGCCCCACGGCCCTCCA
GTCGGTGGAGCAGGTCCAGACCACCCGCACCAAGCTGGTCGTCGGCATGCTCGACAACGTCAAGGGCGCGGCCAAGGTGT
TCACCCACGGGCCCAAGGGCTGGACCGCCCAGACCCTAGACCTGCCGGCCAACAGCGCGATCGGCCTGGGCTCGTCCGAC
GACAAGGGCGAGCGCCTGTTCGTCACCGTCACCGGCTATCTGACGCCGACCACCTTCTGGCTGGCCGACGCCGGTTCGCT
GAAGCTGGAGCAGATCAAGGCCTCGCCGGCCCGCTTCGACGCCTCGACCCACGTGGTCGAGCAGTTCGAGGCGACCAGCA
CGGATGGAACCAAGGTTCCCTATTTCGTCGTGCGGCCCAAGGACGTGAAGTACGATGGTTCGGCCCCGACCCTGCTCTAC
GCCTATGGCGGTTTCCAGGTGCCTCTGACGCCCGGCTATTCGGGCACGATGGGCAAGCTGTGGCTGGAGCGCGGCGGCGT
CTATGTGGTGGCCAATATCCGGGGCGGCGGCGAGTTCGGTCCGGCCTGGCACTCGGCCGGTCTCAAGGAGAACCGCCAGA
AGGTCTATGACGACTTCTTCGCCGTCTCGCAGGACCTGATCACCCGCAAGATCACCTCGCCGCGTCGCCTGGGCATCATG
GGCGGTTCGAACGGTGGTCTTCTGATGGGCGTCGCCCTGACCCAGCGGCCCGAGCTCTACAACGCCATCGTCGTGCAGGT
GCCGCTGTTCGACATGATCCGCTACAGCCAGATCGGCGCGGGCGCGTCGTGGGTGGGCGAATACGGCGACCCGGCCATTC
CGTCGGAGCTGGCCGTGATCGCCAAGTACGACCCCTATTCCAACCTCAAGGCCGGCCAGAAGTACCCCGAGGTGTTCATC
GAGACCTCGACCAAGGACGATCGCGTGCACCCGGCCCACGCTCGCAAGGCCGCCGCGCGCCTGATGGAGCTGGGCTATCC
GGTGCTCTACTACGAGAATGTCGACGGGGGTCACGCGGCCAGCGCCAACCTGGCCGAGACGGCGCGCCGCCAGGCGCTGG
AATATGTCTATCTGACGCGCAAGCTGATGGACTGA

Upstream 100 bases:

>100_bases
CGCCCGCCGCTGGGCCCTGCACTACACCCACCTGTCGCGCCAGTTGATGGATCACTAGATGCGTAAGTCCCTGCTTGCCC
TCCTCGCCTCCACGAGTCTG

Downstream 100 bases:

>100_bases
CACGGCCGGCGCCGCTCGCCCTTAAGGAGAGGCGAGCGGCGCAAAGCCTCGCATACCGGCGTCCCGTCAACCCTAAGGCA
ATCTCTCGGCATCAGAGTGC

Product: prolyl oligopeptidase family protein

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MTLTPAIAADTAKPAGKPAAEARTPLSELGKDDPYLWMEEIEGTRAMDWAKAQNARSLPVLQGDARYADLESKALAILNA
KDRVPGVSFAGDGSLRNFWQDKDHVRGIWRKTTLDSYRTAEPAWETILDIDALTKAENANWVFKGASCLPPEDTRCLITL
SNGGKDAVTVREFDTTTKTFVPGGFELPEGKQNYTWLDKDTLLVGREWKPGELTKSGYAYVLKALKRGQPLDQAKEVFRG
VETDVSVSPFVLRDADGKAVAVMATRGVTFFESETYLIEGDKPVKLDLPLKSSIQGYVDGQMVVLMEQDWPAKGFKVGDL
ISFDLAKLKAAPDKAVATLVLRPTALQSVEQVQTTRTKLVVGMLDNVKGAAKVFTHGPKGWTAQTLDLPANSAIGLGSSD
DKGERLFVTVTGYLTPTTFWLADAGSLKLEQIKASPARFDASTHVVEQFEATSTDGTKVPYFVVRPKDVKYDGSAPTLLY
AYGGFQVPLTPGYSGTMGKLWLERGGVYVVANIRGGGEFGPAWHSAGLKENRQKVYDDFFAVSQDLITRKITSPRRLGIM
GGSNGGLLMGVALTQRPELYNAIVVQVPLFDMIRYSQIGAGASWVGEYGDPAIPSELAVIAKYDPYSNLKAGQKYPEVFI
ETSTKDDRVHPAHARKAAARLMELGYPVLYYENVDGGHAASANLAETARRQALEYVYLTRKLMD

Sequences:

>Translated_704_residues
MTLTPAIAADTAKPAGKPAAEARTPLSELGKDDPYLWMEEIEGTRAMDWAKAQNARSLPVLQGDARYADLESKALAILNA
KDRVPGVSFAGDGSLRNFWQDKDHVRGIWRKTTLDSYRTAEPAWETILDIDALTKAENANWVFKGASCLPPEDTRCLITL
SNGGKDAVTVREFDTTTKTFVPGGFELPEGKQNYTWLDKDTLLVGREWKPGELTKSGYAYVLKALKRGQPLDQAKEVFRG
VETDVSVSPFVLRDADGKAVAVMATRGVTFFESETYLIEGDKPVKLDLPLKSSIQGYVDGQMVVLMEQDWPAKGFKVGDL
ISFDLAKLKAAPDKAVATLVLRPTALQSVEQVQTTRTKLVVGMLDNVKGAAKVFTHGPKGWTAQTLDLPANSAIGLGSSD
DKGERLFVTVTGYLTPTTFWLADAGSLKLEQIKASPARFDASTHVVEQFEATSTDGTKVPYFVVRPKDVKYDGSAPTLLY
AYGGFQVPLTPGYSGTMGKLWLERGGVYVVANIRGGGEFGPAWHSAGLKENRQKVYDDFFAVSQDLITRKITSPRRLGIM
GGSNGGLLMGVALTQRPELYNAIVVQVPLFDMIRYSQIGAGASWVGEYGDPAIPSELAVIAKYDPYSNLKAGQKYPEVFI
ETSTKDDRVHPAHARKAAARLMELGYPVLYYENVDGGHAASANLAETARRQALEYVYLTRKLMD
>Mature_703_residues
TLTPAIAADTAKPAGKPAAEARTPLSELGKDDPYLWMEEIEGTRAMDWAKAQNARSLPVLQGDARYADLESKALAILNAK
DRVPGVSFAGDGSLRNFWQDKDHVRGIWRKTTLDSYRTAEPAWETILDIDALTKAENANWVFKGASCLPPEDTRCLITLS
NGGKDAVTVREFDTTTKTFVPGGFELPEGKQNYTWLDKDTLLVGREWKPGELTKSGYAYVLKALKRGQPLDQAKEVFRGV
ETDVSVSPFVLRDADGKAVAVMATRGVTFFESETYLIEGDKPVKLDLPLKSSIQGYVDGQMVVLMEQDWPAKGFKVGDLI
SFDLAKLKAAPDKAVATLVLRPTALQSVEQVQTTRTKLVVGMLDNVKGAAKVFTHGPKGWTAQTLDLPANSAIGLGSSDD
KGERLFVTVTGYLTPTTFWLADAGSLKLEQIKASPARFDASTHVVEQFEATSTDGTKVPYFVVRPKDVKYDGSAPTLLYA
YGGFQVPLTPGYSGTMGKLWLERGGVYVVANIRGGGEFGPAWHSAGLKENRQKVYDDFFAVSQDLITRKITSPRRLGIMG
GSNGGLLMGVALTQRPELYNAIVVQVPLFDMIRYSQIGAGASWVGEYGDPAIPSELAVIAKYDPYSNLKAGQKYPEVFIE
TSTKDDRVHPAHARKAAARLMELGYPVLYYENVDGGHAASANLAETARRQALEYVYLTRKLMD

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=379, Percent_Identity=33.245382585752, Blast_Score=208, Evalue=2e-53,
Organism=Homo sapiens, GI284172420, Length=211, Percent_Identity=27.0142180094787, Blast_Score=80, Evalue=9e-15,
Organism=Homo sapiens, GI284172413, Length=211, Percent_Identity=27.0142180094787, Blast_Score=80, Evalue=9e-15,
Organism=Homo sapiens, GI70778815, Length=211, Percent_Identity=27.0142180094787, Blast_Score=80, Evalue=9e-15,
Organism=Homo sapiens, GI284172438, Length=211, Percent_Identity=27.0142180094787, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI284172431, Length=211, Percent_Identity=27.0142180094787, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI108860686, Length=211, Percent_Identity=27.0142180094787, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI108860692, Length=199, Percent_Identity=26.1306532663317, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788150, Length=302, Percent_Identity=29.4701986754967, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24583414, Length=316, Percent_Identity=35.7594936708861, Blast_Score=192, Evalue=6e-49,
Organism=Drosophila melanogaster, GI221510989, Length=277, Percent_Identity=35.0180505415162, Blast_Score=173, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 76910; Mature: 76779

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTPAIAADTAKPAGKPAAEARTPLSELGKDDPYLWMEEIEGTRAMDWAKAQNARSLPV
CCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCCEEEHHHCCCCCCHHHHHHCCCCCCCE
LQGDARYADLESKALAILNAKDRVPGVSFAGDGSLRNFWQDKDHVRGIWRKTTLDSYRTA
EECCCCEECCCCCEEEEEECCCCCCCCEECCCCCHHHHHCCCHHHHHHHHHCCCCCCCCC
EPAWETILDIDALTKAENANWVFKGASCLPPEDTRCLITLSNGGKDAVTVREFDTTTKTF
CHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCEE
VPGGFELPEGKQNYTWLDKDTLLVGREWKPGELTKSGYAYVLKALKRGQPLDQAKEVFRG
CCCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCC
VETDVSVSPFVLRDADGKAVAVMATRGVTFFESETYLIEGDKPVKLDLPLKSSIQGYVDG
CCCCCCCCCEEEECCCCCEEEEEEECCEEEEECCEEEEECCCCEEEECCHHHCCCEEECC
QMVVLMEQDWPAKGFKVGDLISFDLAKLKAAPDKAVATLVLRPTALQSVEQVQTTRTKLV
EEEEEEECCCCCCCCEECCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHEE
VGMLDNVKGAAKVFTHGPKGWTAQTLDLPANSAIGLGSSDDKGERLFVTVTGYLTPTTFW
EEHHHCCCCHHHHEECCCCCCEEEEEECCCCCEECCCCCCCCCCEEEEEEEECCCCCEEE
LADAGSLKLEQIKASPARFDASTHVVEQFEATSTDGTKVPYFVVRPKDVKYDGSAPTLLY
EECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCEEECCCCCEEEE
AYGGFQVPLTPGYSGTMGKLWLERGGVYVVANIRGGGEFGPAWHSAGLKENRQKVYDDFF
EECCEEEEECCCCCCCHHHHHEECCCEEEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHH
AVSQDLITRKITSPRRLGIMGGSNGGLLMGVALTQRPELYNAIVVQVPLFDMIRYSQIGA
HHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCHHCEEEEEECHHHHHHHHHCCC
GASWVGEYGDPAIPSELAVIAKYDPYSNLKAGQKYPEVFIETSTKDDRVHPAHARKAAAR
CHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHH
LMELGYPVLYYENVDGGHAASANLAETARRQALEYVYLTRKLMD
HHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLTPAIAADTAKPAGKPAAEARTPLSELGKDDPYLWMEEIEGTRAMDWAKAQNARSLPV
CCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCCEEEHHHCCCCCCHHHHHHCCCCCCCE
LQGDARYADLESKALAILNAKDRVPGVSFAGDGSLRNFWQDKDHVRGIWRKTTLDSYRTA
EECCCCEECCCCCEEEEEECCCCCCCCEECCCCCHHHHHCCCHHHHHHHHHCCCCCCCCC
EPAWETILDIDALTKAENANWVFKGASCLPPEDTRCLITLSNGGKDAVTVREFDTTTKTF
CHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCEE
VPGGFELPEGKQNYTWLDKDTLLVGREWKPGELTKSGYAYVLKALKRGQPLDQAKEVFRG
CCCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCC
VETDVSVSPFVLRDADGKAVAVMATRGVTFFESETYLIEGDKPVKLDLPLKSSIQGYVDG
CCCCCCCCCEEEECCCCCEEEEEEECCEEEEECCEEEEECCCCEEEECCHHHCCCEEECC
QMVVLMEQDWPAKGFKVGDLISFDLAKLKAAPDKAVATLVLRPTALQSVEQVQTTRTKLV
EEEEEEECCCCCCCCEECCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHEE
VGMLDNVKGAAKVFTHGPKGWTAQTLDLPANSAIGLGSSDDKGERLFVTVTGYLTPTTFW
EEHHHCCCCHHHHEECCCCCCEEEEEECCCCCEECCCCCCCCCCEEEEEEEECCCCCEEE
LADAGSLKLEQIKASPARFDASTHVVEQFEATSTDGTKVPYFVVRPKDVKYDGSAPTLLY
EECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCEEECCCCCEEEE
AYGGFQVPLTPGYSGTMGKLWLERGGVYVVANIRGGGEFGPAWHSAGLKENRQKVYDDFF
EECCEEEEECCCCCCCHHHHHEECCCEEEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHH
AVSQDLITRKITSPRRLGIMGGSNGGLLMGVALTQRPELYNAIVVQVPLFDMIRYSQIGA
HHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCHHCEEEEEECHHHHHHHHHCCC
GASWVGEYGDPAIPSELAVIAKYDPYSNLKAGQKYPEVFIETSTKDDRVHPAHARKAAAR
CHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHH
LMELGYPVLYYENVDGGHAASANLAETARRQALEYVYLTRKLMD
HHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]