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The map label for this gene is mltB [H]

Identifier: 16127552

GI number: 16127552

Start: 3568329

End: 3569630

Strand: Direct

Name: mltB [H]

Synonym: CC_3322

Alternate gene names: 16127552

Gene position: 3568329-3569630 (Clockwise)

Preceding gene: 16127548

Following gene: 16127554

Centisome position: 88.83

GC content: 70.2

Gene sequence:

>1302_bases
ATGGCTATGGATCGTCGCGTCTTTCTTGTTCTTCTGCTCGCCGGCTGCGCGGACACCTCACCGCAAGGGCCGCTGACCGC
CATCAGCCCTGCGCCGCCGCCTGCGCCCACGCCTCAAACGACCGTCACCCCCGCGCCAACGCCGCAGACACCGGTGCCTG
CGGGCGAACTGGCCTTCGACACCTGGATGCAGGATTTCCGTGGCCGAGCCATCGCGGCGGGACTGTCGCCCGACCTTCTG
GACCGCGAGCTGGGCGGGGTGATTCCCGATCCCAGGGTGATCAGCCTCGACAGCCGCCAGCCGGAGTTCTCCAAGCCCGT
CGGCGACTATATCCGCGGCGTGATCAGCGAGGACCGCGTCGCGATCGGCCGCAGCAAGCGCGATCAGCTAGACTTCCTCT
CACAGATCGAGTCCCGGTACGGCGTGCCGGGTGACATCCTGCTGGCGGTGTGGGCCATGGAGTCCGCGTTCGGGCAGCTG
CAAGGCAATTTCGACGTGGTCCGCTCGATGGTCAGCCTTGCCGCCGACGGCCGTCGACGGGCCTGGGCCGAGGGCGAGCT
GATCGCCGCCCTGAAGATCATCGACTCCGGCGAGGTCACGCGCGCCCAGCTGAAAGGCTCGTGGGCCGGCGCCATGGGCC
AGACCCAGTTCATCCCGACGAGCTTCAGGGCCACAGCCGTGGACTTCGACGGCGACGGCCGCCGCGACATCTGGGGCTCG
GACGCCGACGCCCTGGCCTCAGCCGCCAATCTGCTGGTGCGCGGCGGCTGGAAGCGGAACGTCGGCTGGGCCAGGGAGGT
CATCCTGCCGCCAGGCTTCGACTACTCGGTCACTGAGGGCCCCAGGGAAATCCCGGCCTGGTGGGAAGCCCGCGGCGTGC
GCCGCGCGGATGGCCTGCCTTGGACCGCTCAGGACGCGGCGTCGCCCGCCATGCTGATCCTGCCGGCCGGCGCGACGGGA
CCGGCGTTCCTCGCCCTGCCCAATCATTTTGCGATCAGGACCTACAACAACTCCACCTCCTATGCGCTGGGCATCGGCCT
GCTGGCCGACCGGTTCGCCGGCGGCGCGCCGCCGATCACGCCCTGGCCGGTGGAGACCCCGCTGTCGATGTCGGATCGCA
TGGCCGCCCAGATCGCCCTGGCGCGCGTCGGTTTCAATCCCGGCCCCGCCGACGGTGTGATCGGCGCCGGCACGCGACGG
GCGCTGCGCGCCTGGCAGCAGAGCCAGCAATTGCCCGCCGACGGCTACCTGTCCAACGACATGGTCGCGCGGCTGAAGGC
CCAGGCCGGGATCAGCCCGTAG

Upstream 100 bases:

>100_bases
CAATGCGCTCCTGGGCGTTTCGCGGCGCAGGGCGGGAACTTCACCCGTCTGGCGCGCTTGTGATTGAAGCTTTCCCGCGA
ACCCCGGTAGCTTCCTTCGC

Downstream 100 bases:

>100_bases
CGATCTCCATCCAGATCGACCGGCGCTCGCCCGGACACAGCGTGACCATTCCCGTTTCACCCTCCCCAAAGGGGTTGGGG
CGGCTCGCACAGGGCTCCAC

Product: peptidoglycan-binding protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MAMDRRVFLVLLLAGCADTSPQGPLTAISPAPPPAPTPQTTVTPAPTPQTPVPAGELAFDTWMQDFRGRAIAAGLSPDLL
DRELGGVIPDPRVISLDSRQPEFSKPVGDYIRGVISEDRVAIGRSKRDQLDFLSQIESRYGVPGDILLAVWAMESAFGQL
QGNFDVVRSMVSLAADGRRRAWAEGELIAALKIIDSGEVTRAQLKGSWAGAMGQTQFIPTSFRATAVDFDGDGRRDIWGS
DADALASAANLLVRGGWKRNVGWAREVILPPGFDYSVTEGPREIPAWWEARGVRRADGLPWTAQDAASPAMLILPAGATG
PAFLALPNHFAIRTYNNSTSYALGIGLLADRFAGGAPPITPWPVETPLSMSDRMAAQIALARVGFNPGPADGVIGAGTRR
ALRAWQQSQQLPADGYLSNDMVARLKAQAGISP

Sequences:

>Translated_433_residues
MAMDRRVFLVLLLAGCADTSPQGPLTAISPAPPPAPTPQTTVTPAPTPQTPVPAGELAFDTWMQDFRGRAIAAGLSPDLL
DRELGGVIPDPRVISLDSRQPEFSKPVGDYIRGVISEDRVAIGRSKRDQLDFLSQIESRYGVPGDILLAVWAMESAFGQL
QGNFDVVRSMVSLAADGRRRAWAEGELIAALKIIDSGEVTRAQLKGSWAGAMGQTQFIPTSFRATAVDFDGDGRRDIWGS
DADALASAANLLVRGGWKRNVGWAREVILPPGFDYSVTEGPREIPAWWEARGVRRADGLPWTAQDAASPAMLILPAGATG
PAFLALPNHFAIRTYNNSTSYALGIGLLADRFAGGAPPITPWPVETPLSMSDRMAAQIALARVGFNPGPADGVIGAGTRR
ALRAWQQSQQLPADGYLSNDMVARLKAQAGISP
>Mature_432_residues
AMDRRVFLVLLLAGCADTSPQGPLTAISPAPPPAPTPQTTVTPAPTPQTPVPAGELAFDTWMQDFRGRAIAAGLSPDLLD
RELGGVIPDPRVISLDSRQPEFSKPVGDYIRGVISEDRVAIGRSKRDQLDFLSQIESRYGVPGDILLAVWAMESAFGQLQ
GNFDVVRSMVSLAADGRRRAWAEGELIAALKIIDSGEVTRAQLKGSWAGAMGQTQFIPTSFRATAVDFDGDGRRDIWGSD
ADALASAANLLVRGGWKRNVGWAREVILPPGFDYSVTEGPREIPAWWEARGVRRADGLPWTAQDAASPAMLILPAGATGP
AFLALPNHFAIRTYNNSTSYALGIGLLADRFAGGAPPITPWPVETPLSMSDRMAAQIALARVGFNPGPADGVIGAGTRRA
LRAWQQSQQLPADGYLSNDMVARLKAQAGISP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=245, Percent_Identity=30.2040816326531, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 46170; Mature: 46039

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMDRRVFLVLLLAGCADTSPQGPLTAISPAPPPAPTPQTTVTPAPTPQTPVPAGELAFD
CCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCCCCCCCCHHHHH
TWMQDFRGRAIAAGLSPDLLDRELGGVIPDPRVISLDSRQPEFSKPVGDYIRGVISEDRV
HHHHHHCCCEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCE
AIGRSKRDQLDFLSQIESRYGVPGDILLAVWAMESAFGQLQGNFDVVRSMVSLAADGRRR
ECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
AWAEGELIAALKIIDSGEVTRAQLKGSWAGAMGQTQFIPTSFRATAVDFDGDGRRDIWGS
CCCCCCEEEEEEEECCCCEEEHHHCCCCCCCCCCCEECCCCCEEEEEECCCCCCCCCCCC
DADALASAANLLVRGGWKRNVGWAREVILPPGFDYSVTEGPREIPAWWEARGVRRADGLP
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
WTAQDAASPAMLILPAGATGPAFLALPNHFAIRTYNNSTSYALGIGLLADRFAGGAPPIT
CCCHHCCCCEEEEEECCCCCCEEEECCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCCCC
PWPVETPLSMSDRMAAQIALARVGFNPGPADGVIGAGTRRALRAWQQSQQLPADGYLSND
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHH
MVARLKAQAGISP
HHHHHHHHCCCCC
>Mature Secondary Structure 
AMDRRVFLVLLLAGCADTSPQGPLTAISPAPPPAPTPQTTVTPAPTPQTPVPAGELAFD
CCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCCCCCCCCHHHHH
TWMQDFRGRAIAAGLSPDLLDRELGGVIPDPRVISLDSRQPEFSKPVGDYIRGVISEDRV
HHHHHHCCCEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCE
AIGRSKRDQLDFLSQIESRYGVPGDILLAVWAMESAFGQLQGNFDVVRSMVSLAADGRRR
ECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
AWAEGELIAALKIIDSGEVTRAQLKGSWAGAMGQTQFIPTSFRATAVDFDGDGRRDIWGS
CCCCCCEEEEEEEECCCCEEEHHHCCCCCCCCCCCEECCCCCEEEEEECCCCCCCCCCCC
DADALASAANLLVRGGWKRNVGWAREVILPPGFDYSVTEGPREIPAWWEARGVRRADGLP
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
WTAQDAASPAMLILPAGATGPAFLALPNHFAIRTYNNSTSYALGIGLLADRFAGGAPPIT
CCCHHCCCCEEEEEECCCCCCEEEECCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCCCC
PWPVETPLSMSDRMAAQIALARVGFNPGPADGVIGAGTRRALRAWQQSQQLPADGYLSND
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHH
MVARLKAQAGISP
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]