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The map label for this gene is pbpF [H]

Identifier: 16127507

GI number: 16127507

Start: 3532200

End: 3534401

Strand: Direct

Name: pbpF [H]

Synonym: CC_3277

Alternate gene names: 16127507

Gene position: 3532200-3534401 (Clockwise)

Preceding gene: 16127505

Following gene: 16127511

Centisome position: 87.93

GC content: 68.3

Gene sequence:

>2202_bases
GTGAACGACTGGACGCTGCCGCCCTACAAGTTCGACGACGGGAAGAGTCCCGGAGAGCCGCCGAAGCCCGGTCCGGCCTC
GGCGAGCGATGGCGTCTCGCAAGATCCCTGGGCGCCGCAGGGGCCTGATCCGTTCCGCCTGTCTAGCGACGCCGCGACAC
CTCCGCCGCCGCCTGAGCCCCCGCCGGAGGAGCCCTTCCGCGCCGACCTCAAGCACGCTGCGGCCAAGAAGAAGGCCCGC
AAGTGGGGCTGGGTGTGGGGCACTCTGCTGGTCGGCTTTCTTCTTGCCGTCCTGAGCGTCGCCGGCGGCGGCGCCTATGT
CTGGTTCAAGTACCTGAAGGACACCCCCGCCCTGCCCTCACGCGAGGCGCTGTTTGCGGTGAACCGGGCGCCGGGCATTC
GCTTCGAGGACCGCAACGGGCAGGTGATCGCCACCCGGGGTCCGCGCTATGGCCAGCGCATCACCCTGGGCACGGTGCCC
AACTATGTGCCCATGGCGTTCCTGGCGGCCGAGGACCGGCGCTTCTACCAGCACGGCGCCATCGACGTGCAAGGCATCGC
CCGGGCGGCCTGGATCAACTGGCGCGCGGGCAAGACCCGCCAAGGCGCCTCGACCCTGACCCAACAACTGGCAAAGGGCC
TGTTCCTGACCCCCGATCGCGTGGTGAAGCGTAAGCTGCAGGAGATGCTGCTGGCCTACAAGCTGGAGCAGATTCTCACC
AAGGACGAGATCCTCGAGCTCTATCTGAACCGTATCTATTTCGGCGCGGGCACCTATGGGATCGACGGCGCGTCCCAGAC
CTATTTCGGCAAGCCCGCCAGCCAGCTCACCCTGTCGGAAGCCGCCCTGCTGGCCAGCCTGCCCAAGGCGCCGTCGCGCC
TGGCCCTGACCCGCGACATGGAGCGGGCCCTGGCCCGCTCTCGCCTGATCCTGGCCAATATGCGCAAGGAAGGCTGGATC
ACGCCCGAGCAGGAAAGCCGCGCGCTCGACGACACGCCGCGCCTGTCGCCGATGGCGCTGCAGGACGAGGGCGACTACGG
CTGGGTGCTCGACTACGCCACCGCCGAGGCGGTGAAGATCGCCGGTCAGAACGCGCCGGACCTCGTGGTGCGCCTGACGA
TCGACCCCTTGCTCCAGAGCGAGGGCGCCGAGGTTGTCCGCCAGACCATGGCCACCGAGACCACCCGCTCAGGCGCCAGC
CAGGCGGCCCTGTTGTCGCTGTCGGCCGATGGGGCCATCCGCGCCATGGTCGGCGGGACCGACTATTCGGAAAGCCCGTT
CAACCGCGCTGTCCAGGCCAAGCGCCAGCCGGGCTCGACCTTCAAGCCCTTTGTCTACGCCGCCGCCGTCGAGAAGGGCG
TCCTGCCGACCGACATGCGGGTCGACGAGCCCGTCAAGTTCGGGACGTGGGAGCCAGAGAACTACAGCGGCGGCTATCGC
GGCCCGATGACCGTCGAAGACGCGCTGGTCACCTCGATCAACACGGTCGCCGTCAAGCTGGGCCAGGAAGTCGGCGGTCC
GGCGATCGGCGACCTCGTCCGCCGCTTCGGCATCACCAGCCTGCCGCCCTCGCCCGACCTGTCGGTGGCCCTGGGCTCAT
ATGAGGTGAACCTCTTGCAGATCACCTCGGGCTTCCAGGTGTTCCAGCAAGGCGGGCTCCGGATCGAGCCCTATGTGATC
GAGTCGATCACCACCCAGGGCGGGCAACAGATCTTCCAGCACCAGCCGCCGCAGGGCGAGCGTCGCGTCTACGACGTCGC
GCACGCCAGCATGATGGTGAAGATGATGAAGAAGGTGGTCACCCAAGGCACCGCCCAGCGCGCCGCCTTCGGTCGCCCGG
TCGCCGGCAAGACCGGCACCAGCCAGAACTGGCGCGACGCCTGGTTCGTGGGCTTTACCCCCGACTATGTGACCGGCGTC
TGGGTCGGCAACGACGACGAAAAGCCGATGAACAAGGTGGTCGGCGGCGACATTCCCGCCAGCATCTGGCGGCGCTTCAT
GATGACCGCGCATCAGACCCTGGCGGTGCGCGACTTCGAGTGGCTGCTGCCCGACCCCGCGCCGCAGAGCGAGCCTGATC
CGCGCAACGGCTTCTACGAGACGCTATCGGCCGAGTTCTCGCGCGCAGCCTCGGAACTGGAGACAACTACGCCTGTTGCG
CCCGCGCCGGGGCAGCCGCCCCCGGACAACCTGCCCTACTAG

Upstream 100 bases:

>100_bases
TTTCGGCGAGCGTCTTCGTCTGCCAGAAAGGTTTGCGTGGCGTCATGACGCCGTCCTAAACCCGATTTATCCGGCGACCA
AACAGAGCCTGACGCAAACC

Downstream 100 bases:

>100_bases
AGCGCGTTAGGTTTAAGTGTGAGCGGTTAAACCGCTCGAACGCGCTCTAAGTCTATGAATTAGAGCCTGTTTATCTGGTT
TAGATGATTCCATCTAAACC

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MNDWTLPPYKFDDGKSPGEPPKPGPASASDGVSQDPWAPQGPDPFRLSSDAATPPPPPEPPPEEPFRADLKHAAAKKKAR
KWGWVWGTLLVGFLLAVLSVAGGGAYVWFKYLKDTPALPSREALFAVNRAPGIRFEDRNGQVIATRGPRYGQRITLGTVP
NYVPMAFLAAEDRRFYQHGAIDVQGIARAAWINWRAGKTRQGASTLTQQLAKGLFLTPDRVVKRKLQEMLLAYKLEQILT
KDEILELYLNRIYFGAGTYGIDGASQTYFGKPASQLTLSEAALLASLPKAPSRLALTRDMERALARSRLILANMRKEGWI
TPEQESRALDDTPRLSPMALQDEGDYGWVLDYATAEAVKIAGQNAPDLVVRLTIDPLLQSEGAEVVRQTMATETTRSGAS
QAALLSLSADGAIRAMVGGTDYSESPFNRAVQAKRQPGSTFKPFVYAAAVEKGVLPTDMRVDEPVKFGTWEPENYSGGYR
GPMTVEDALVTSINTVAVKLGQEVGGPAIGDLVRRFGITSLPPSPDLSVALGSYEVNLLQITSGFQVFQQGGLRIEPYVI
ESITTQGGQQIFQHQPPQGERRVYDVAHASMMVKMMKKVVTQGTAQRAAFGRPVAGKTGTSQNWRDAWFVGFTPDYVTGV
WVGNDDEKPMNKVVGGDIPASIWRRFMMTAHQTLAVRDFEWLLPDPAPQSEPDPRNGFYETLSAEFSRAASELETTTPVA
PAPGQPPPDNLPY

Sequences:

>Translated_733_residues
MNDWTLPPYKFDDGKSPGEPPKPGPASASDGVSQDPWAPQGPDPFRLSSDAATPPPPPEPPPEEPFRADLKHAAAKKKAR
KWGWVWGTLLVGFLLAVLSVAGGGAYVWFKYLKDTPALPSREALFAVNRAPGIRFEDRNGQVIATRGPRYGQRITLGTVP
NYVPMAFLAAEDRRFYQHGAIDVQGIARAAWINWRAGKTRQGASTLTQQLAKGLFLTPDRVVKRKLQEMLLAYKLEQILT
KDEILELYLNRIYFGAGTYGIDGASQTYFGKPASQLTLSEAALLASLPKAPSRLALTRDMERALARSRLILANMRKEGWI
TPEQESRALDDTPRLSPMALQDEGDYGWVLDYATAEAVKIAGQNAPDLVVRLTIDPLLQSEGAEVVRQTMATETTRSGAS
QAALLSLSADGAIRAMVGGTDYSESPFNRAVQAKRQPGSTFKPFVYAAAVEKGVLPTDMRVDEPVKFGTWEPENYSGGYR
GPMTVEDALVTSINTVAVKLGQEVGGPAIGDLVRRFGITSLPPSPDLSVALGSYEVNLLQITSGFQVFQQGGLRIEPYVI
ESITTQGGQQIFQHQPPQGERRVYDVAHASMMVKMMKKVVTQGTAQRAAFGRPVAGKTGTSQNWRDAWFVGFTPDYVTGV
WVGNDDEKPMNKVVGGDIPASIWRRFMMTAHQTLAVRDFEWLLPDPAPQSEPDPRNGFYETLSAEFSRAASELETTTPVA
PAPGQPPPDNLPY
>Mature_733_residues
MNDWTLPPYKFDDGKSPGEPPKPGPASASDGVSQDPWAPQGPDPFRLSSDAATPPPPPEPPPEEPFRADLKHAAAKKKAR
KWGWVWGTLLVGFLLAVLSVAGGGAYVWFKYLKDTPALPSREALFAVNRAPGIRFEDRNGQVIATRGPRYGQRITLGTVP
NYVPMAFLAAEDRRFYQHGAIDVQGIARAAWINWRAGKTRQGASTLTQQLAKGLFLTPDRVVKRKLQEMLLAYKLEQILT
KDEILELYLNRIYFGAGTYGIDGASQTYFGKPASQLTLSEAALLASLPKAPSRLALTRDMERALARSRLILANMRKEGWI
TPEQESRALDDTPRLSPMALQDEGDYGWVLDYATAEAVKIAGQNAPDLVVRLTIDPLLQSEGAEVVRQTMATETTRSGAS
QAALLSLSADGAIRAMVGGTDYSESPFNRAVQAKRQPGSTFKPFVYAAAVEKGVLPTDMRVDEPVKFGTWEPENYSGGYR
GPMTVEDALVTSINTVAVKLGQEVGGPAIGDLVRRFGITSLPPSPDLSVALGSYEVNLLQITSGFQVFQQGGLRIEPYVI
ESITTQGGQQIFQHQPPQGERRVYDVAHASMMVKMMKKVVTQGTAQRAAFGRPVAGKTGTSQNWRDAWFVGFTPDYVTGV
WVGNDDEKPMNKVVGGDIPASIWRRFMMTAHQTLAVRDFEWLLPDPAPQSEPDPRNGFYETLSAEFSRAASELETTTPVA
PAPGQPPPDNLPY

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=545, Percent_Identity=31.5596330275229, Blast_Score=209, Evalue=7e-55,
Organism=Escherichia coli, GI1788867, Length=581, Percent_Identity=30.9810671256454, Blast_Score=202, Evalue=6e-53,
Organism=Escherichia coli, GI87082258, Length=232, Percent_Identity=43.9655172413793, Blast_Score=196, Evalue=6e-51,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=38.7878787878788, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 80090; Mature: 80090

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDWTLPPYKFDDGKSPGEPPKPGPASASDGVSQDPWAPQGPDPFRLSSDAATPPPPPEP
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCC
PPEEPFRADLKHAAAKKKARKWGWVWGTLLVGFLLAVLSVAGGGAYVWFKYLKDTPALPS
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCC
REALFAVNRAPGIRFEDRNGQVIATRGPRYGQRITLGTVPNYVPMAFLAAEDRRFYQHGA
CCCEEEEECCCCCEEECCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCC
IDVQGIARAAWINWRAGKTRQGASTLTQQLAKGLFLTPDRVVKRKLQEMLLAYKLEQILT
EEHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDEILELYLNRIYFGAGTYGIDGASQTYFGKPASQLTLSEAALLASLPKAPSRLALTRDM
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ERALARSRLILANMRKEGWITPEQESRALDDTPRLSPMALQDEGDYGWVLDYATAEAVKI
HHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCEEEECCCCCEEEEECCHHHHHHC
AGQNAPDLVVRLTIDPLLQSEGAEVVRQTMATETTRSGASQAALLSLSADGAIRAMVGGT
CCCCCCCEEEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCC
DYSESPFNRAVQAKRQPGSTFKPFVYAAAVEKGVLPTDMRVDEPVKFGTWEPENYSGGYR
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GPMTVEDALVTSINTVAVKLGQEVGGPAIGDLVRRFGITSLPPSPDLSVALGSYEVNLLQ
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEE
ITSGFQVFQQGGLRIEPYVIESITTQGGQQIFQHQPPQGERRVYDVAHASMMVKMMKKVV
ECCHHHHHHCCCCEECCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
TQGTAQRAAFGRPVAGKTGTSQNWRDAWFVGFTPDYVTGVWVGNDDEKPMNKVVGGDIPA
HCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHCCCCCCH
SIWRRFMMTAHQTLAVRDFEWLLPDPAPQSEPDPRNGFYETLSAEFSRAASELETTTPVA
HHHHHHHHHHHHHHEEECHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
PAPGQPPPDNLPY
CCCCCCCCCCCCC
>Mature Secondary Structure
MNDWTLPPYKFDDGKSPGEPPKPGPASASDGVSQDPWAPQGPDPFRLSSDAATPPPPPEP
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCC
PPEEPFRADLKHAAAKKKARKWGWVWGTLLVGFLLAVLSVAGGGAYVWFKYLKDTPALPS
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCC
REALFAVNRAPGIRFEDRNGQVIATRGPRYGQRITLGTVPNYVPMAFLAAEDRRFYQHGA
CCCEEEEECCCCCEEECCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCC
IDVQGIARAAWINWRAGKTRQGASTLTQQLAKGLFLTPDRVVKRKLQEMLLAYKLEQILT
EEHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDEILELYLNRIYFGAGTYGIDGASQTYFGKPASQLTLSEAALLASLPKAPSRLALTRDM
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ERALARSRLILANMRKEGWITPEQESRALDDTPRLSPMALQDEGDYGWVLDYATAEAVKI
HHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCEEEECCCCCEEEEECCHHHHHHC
AGQNAPDLVVRLTIDPLLQSEGAEVVRQTMATETTRSGASQAALLSLSADGAIRAMVGGT
CCCCCCCEEEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCC
DYSESPFNRAVQAKRQPGSTFKPFVYAAAVEKGVLPTDMRVDEPVKFGTWEPENYSGGYR
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GPMTVEDALVTSINTVAVKLGQEVGGPAIGDLVRRFGITSLPPSPDLSVALGSYEVNLLQ
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEE
ITSGFQVFQQGGLRIEPYVIESITTQGGQQIFQHQPPQGERRVYDVAHASMMVKMMKKVV
ECCHHHHHHCCCCEECCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
TQGTAQRAAFGRPVAGKTGTSQNWRDAWFVGFTPDYVTGVWVGNDDEKPMNKVVGGDIPA
HCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHCCCCCCH
SIWRRFMMTAHQTLAVRDFEWLLPDPAPQSEPDPRNGFYETLSAEFSRAASELETTTPVA
HHHHHHHHHHHHHHEEECHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
PAPGQPPPDNLPY
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]