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The map label for this gene is rpoH [H]

Identifier: 16127328

GI number: 16127328

Start: 3329617

End: 3330513

Strand: Direct

Name: rpoH [H]

Synonym: CC_3098

Alternate gene names: 16127328

Gene position: 3329617-3330513 (Clockwise)

Preceding gene: 16127327

Following gene: 16127329

Centisome position: 82.89

GC content: 64.44

Gene sequence:

>897_bases
ATGAGTAAGATGGCCGTGAATTCTCTATCGGTGATGTCGCCGGACGGCGGCTTGTCGCGTTACCTCACCGAGATCCGCAA
GTTCCCGATGCTCAGCAAGGACGAAGAGTTCATGCTGGCCCAGCGCTGGAAAGAGCATCAGGATCCGCAAGCGGCCCACA
AGATGGTCACCAGCCACCTGCGCCTCGTAGCCAAGATCGCCATGGGCTACCGCGGCTACGGCCTGCCGATCGGCGAGGTG
ATCTCCGAGGGCAATGTCGGCCTGATGCAGGCGGTCAAGAAGTTCGAGCCCGAAAAGGGCTTCCGCCTGGCGACCTACGC
CATGTGGTGGATCCGCGCCTCGATCCAGGAATACATCCTGCGCTCGTGGTCGCTGGTGAAGATGGGCACGACCGCCGCGC
AGAAGAAGCTGTTCTTCAACCTGCGCAAGGCCAAGAGCCAGATCGCCGCCTTCCAGGAAGGCGACCTGCACCCCGACCAG
GTCAGCCAGATCGCCACCAAGCTGGGCGTGCTGGACAGCGAAGTCATCTCGATGAACCGCCGCCTGTCGGGCCCCGACGC
CTCGCTGAACGCGCCGCTGCGCGCCGACGGCGAGAGCGAGTGGCAGGACTGGCTGGCTGACGAGGAGCAGGTCTCCCAGG
AGACCCGCGTCGCCGAGGACGAGGAAAAGTCGCTGCGGATGTCGCTTCTGGAAGAGGCGATGGTCGAGCTGACCGACCGC
GAGCGTCACATCCTGACCGAGCGCCGCCTGAAGGACGATCCGACCACCCTGGAAGAACTCGCCGCCCAATACGGCGTCAG
CCGCGAACGCGTGCGCCAGATCGAGGTCCGCGCGTTCGAGAAGCTGCAGAAGACCATGCGCGAGGCGGCGATCGCCAAGA
ACATGGTCGACGCCTAG

Upstream 100 bases:

>100_bases
TGCGTTACCGCTCTTCAAACTGCCGCAGCGTGAACTATCTACCCTCCTGTGTGGGGGATGATCGCGCGCGATTGGCGAGC
GGATCCGCACGCAAGGGGAT

Downstream 100 bases:

>100_bases
GGCTTTGGCTCATGATCGTGAGACGGAAAGGGCGCTCTTCGGAGCGCCCTTTTTGTTGGGCCAACATCGGCGCCTCTACG
TGCCGGCCAGGCTGTCGCGG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSKMAVNSLSVMSPDGGLSRYLTEIRKFPMLSKDEEFMLAQRWKEHQDPQAAHKMVTSHLRLVAKIAMGYRGYGLPIGEV
ISEGNVGLMQAVKKFEPEKGFRLATYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRKAKSQIAAFQEGDLHPDQ
VSQIATKLGVLDSEVISMNRRLSGPDASLNAPLRADGESEWQDWLADEEQVSQETRVAEDEEKSLRMSLLEEAMVELTDR
ERHILTERRLKDDPTTLEELAAQYGVSRERVRQIEVRAFEKLQKTMREAAIAKNMVDA

Sequences:

>Translated_298_residues
MSKMAVNSLSVMSPDGGLSRYLTEIRKFPMLSKDEEFMLAQRWKEHQDPQAAHKMVTSHLRLVAKIAMGYRGYGLPIGEV
ISEGNVGLMQAVKKFEPEKGFRLATYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRKAKSQIAAFQEGDLHPDQ
VSQIATKLGVLDSEVISMNRRLSGPDASLNAPLRADGESEWQDWLADEEQVSQETRVAEDEEKSLRMSLLEEAMVELTDR
ERHILTERRLKDDPTTLEELAAQYGVSRERVRQIEVRAFEKLQKTMREAAIAKNMVDA
>Mature_297_residues
SKMAVNSLSVMSPDGGLSRYLTEIRKFPMLSKDEEFMLAQRWKEHQDPQAAHKMVTSHLRLVAKIAMGYRGYGLPIGEVI
SEGNVGLMQAVKKFEPEKGFRLATYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRKAKSQIAAFQEGDLHPDQV
SQIATKLGVLDSEVISMNRRLSGPDASLNAPLRADGESEWQDWLADEEQVSQETRVAEDEEKSLRMSLLEEAMVELTDRE
RHILTERRLKDDPTTLEELAAQYGVSRERVRQIEVRAFEKLQKTMREAAIAKNMVDA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=293, Percent_Identity=39.9317406143345, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1789098, Length=270, Percent_Identity=34.4444444444444, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1789448, Length=240, Percent_Identity=31.6666666666667, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34006; Mature: 33875

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKMAVNSLSVMSPDGGLSRYLTEIRKFPMLSKDEEFMLAQRWKEHQDPQAAHKMVTSHL
CCCHHCCCHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
RLVAKIAMGYRGYGLPIGEVISEGNVGLMQAVKKFEPEKGFRLATYAMWWIRASIQEYIL
HHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RSWSLVKMGTTAAQKKLFFNLRKAKSQIAAFQEGDLHPDQVSQIATKLGVLDSEVISMNR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RLSGPDASLNAPLRADGESEWQDWLADEEQVSQETRVAEDEEKSLRMSLLEEAMVELTDR
CCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ERHILTERRLKDDPTTLEELAAQYGVSRERVRQIEVRAFEKLQKTMREAAIAKNMVDA
HHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKMAVNSLSVMSPDGGLSRYLTEIRKFPMLSKDEEFMLAQRWKEHQDPQAAHKMVTSHL
CCHHCCCHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
RLVAKIAMGYRGYGLPIGEVISEGNVGLMQAVKKFEPEKGFRLATYAMWWIRASIQEYIL
HHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RSWSLVKMGTTAAQKKLFFNLRKAKSQIAAFQEGDLHPDQVSQIATKLGVLDSEVISMNR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RLSGPDASLNAPLRADGESEWQDWLADEEQVSQETRVAEDEEKSLRMSLLEEAMVELTDR
CCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ERHILTERRLKDDPTTLEELAAQYGVSRERVRQIEVRAFEKLQKTMREAAIAKNMVDA
HHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]