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The map label for this gene is vapI [H]

Identifier: 16127099

GI number: 16127099

Start: 3091064

End: 3091378

Strand: Direct

Name: vapI [H]

Synonym: CC_2867

Alternate gene names: 16127099

Gene position: 3091064-3091378 (Clockwise)

Preceding gene: 16127098

Following gene: 16127101

Centisome position: 76.95

GC content: 65.71

Gene sequence:

>315_bases
ATGAGCATGGACATCGCTGACGGCAACGATCCCCTCGCCCCGGTCCATCCGGGCGAGATCCTGCTGGAAGAGTTCCTGAA
GCCCTATGGCCTCACGCCCGGTCGGGTCGCGACTCGACTGCACATTGCGCGCCCCCGGATCGAGAAGCTCGTTCGGGGTC
AAACCCCGGTGACCATCGACACCGCGCTGCGCCTGGAGCGCCTGTTCGGCGCCAGCGCGCAGTTCTGGCTCAATCTGCAA
AACCGCTACGACCTGGAAATGGCCAAGCGAGGCGTCGCGCCGGACATCGCCAGCATCGAGCCGTTCGCGGCTTAG

Upstream 100 bases:

>100_bases
TTCCTGCGCCTGCCGGTGACCTGGGCGGCTTAGGGTAGGCGGAGGTCGAGTTCGTGGATTATCCCTGAGCGCCGCTGGGG
CTCAGAACAGGAGGCGACCG

Downstream 100 bases:

>100_bases
AGCCTCGCGCGTCAAAACGGACTCGTTTTGACGCGATAACGAGGCTCTAAAATCAAACACTTAGAGCGTGACTTACGCCG
AAGCCGGTTCCCACTTTCGG

Product: virulence-associated protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 104; Mature: 103

Protein sequence:

>104_residues
MSMDIADGNDPLAPVHPGEILLEEFLKPYGLTPGRVATRLHIARPRIEKLVRGQTPVTIDTALRLERLFGASAQFWLNLQ
NRYDLEMAKRGVAPDIASIEPFAA

Sequences:

>Translated_104_residues
MSMDIADGNDPLAPVHPGEILLEEFLKPYGLTPGRVATRLHIARPRIEKLVRGQTPVTIDTALRLERLFGASAQFWLNLQ
NRYDLEMAKRGVAPDIASIEPFAA
>Mature_103_residues
SMDIADGNDPLAPVHPGEILLEEFLKPYGLTPGRVATRLHIARPRIEKLVRGQTPVTIDTALRLERLFGASAQFWLNLQN
RYDLEMAKRGVAPDIASIEPFAA

Specific function: Unknown

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081745, Length=72, Percent_Identity=38.8888888888889, Blast_Score=61, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 11551; Mature: 11419

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMDIADGNDPLAPVHPGEILLEEFLKPYGLTPGRVATRLHIARPRIEKLVRGQTPVTID
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHH
TALRLERLFGASAQFWLNLQNRYDLEMAKRGVAPDIASIEPFAA
HHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SMDIADGNDPLAPVHPGEILLEEFLKPYGLTPGRVATRLHIARPRIEKLVRGQTPVTID
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHH
TALRLERLFGASAQFWLNLQNRYDLEMAKRGVAPDIASIEPFAA
HHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8169216 [H]