Click here to switch to the map view.

The map label for this gene is pucL [H]

Identifier: 16126843

GI number: 16126843

Start: 2816818

End: 2817390

Strand: Direct

Name: pucL [H]

Synonym: CC_2606

Alternate gene names: 16126843

Gene position: 2816818-2817390 (Clockwise)

Preceding gene: 16126842

Following gene: 16126844

Centisome position: 70.12

GC content: 66.32

Gene sequence:

>573_bases
ATGGGTCCCGCGCATAAAGCGCGGGATGACGGTGAGGGAGGTTGGAGCATGAATTCCCATCCTCCCCTGTCCGTCCAGAC
CCTCAACACCCTCGACCGCAAAGCCTTCACCACCGCCCTGCACTTCGCCTTCGAACTCTCCCCCTGGGTGGTCGAGCGCG
CCTGGGACGCCCGCCCCTTCGCGTCCGTCGAGACGATGCACGCGGCGATGATGGCCGTGCTCGACGCGGCGAGCACCGCC
GACAAGCTGGCGCTGATCCGCGCTCACCCGGAACTGGCCGGCAAGGCCGCGATCGCCAAGCAACTGACCGCCGAGAGCAA
CGCCGAACAGGCCAGCGCGGGTCTGGACAAGCTGACGCCCGACGAGTTCGCGCGCTTCCATGCGCTGAACGCCGCCTACA
ACACGCGCTTCGGCTTTCCCTTCATCATCTGCGTGCGCCTGAACGACAAGGCCTCGATCCTTGAGGCCATGGCCGCGCGC
CTTTCCAACGACGAGGCGAGCGAGATCGCGCAGGCTATCACCCAGATCGGCCTGATCTCCAAGCTTCGCCTGCTCGACGC
GGTGACCCACTGA

Upstream 100 bases:

>100_bases
TCCCCAGTCCCCAAAGCCCGTCATCCCGCGACTTGTTCGCGGGACCCATGGTTCAGCTTTCGCGTGAGCGTGTGACCGTC
CTCTCACCTGCGCCGGACAA

Downstream 100 bases:

>100_bases
TGGACTACGATTTCGCCGGTTGGATCGGCATGGCGCTGCGCTGGCTGCACGTGGTGGCGGGCGTGGCCTGGATCGGCGCG
TCGTTCTATTTCGTCTGGCT

Product: hypothetical protein

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MGPAHKARDDGEGGWSMNSHPPLSVQTLNTLDRKAFTTALHFAFELSPWVVERAWDARPFASVETMHAAMMAVLDAASTA
DKLALIRAHPELAGKAAIAKQLTAESNAEQASAGLDKLTPDEFARFHALNAAYNTRFGFPFIICVRLNDKASILEAMAAR
LSNDEASEIAQAITQIGLISKLRLLDAVTH

Sequences:

>Translated_190_residues
MGPAHKARDDGEGGWSMNSHPPLSVQTLNTLDRKAFTTALHFAFELSPWVVERAWDARPFASVETMHAAMMAVLDAASTA
DKLALIRAHPELAGKAAIAKQLTAESNAEQASAGLDKLTPDEFARFHALNAAYNTRFGFPFIICVRLNDKASILEAMAAR
LSNDEASEIAQAITQIGLISKLRLLDAVTH
>Mature_189_residues
GPAHKARDDGEGGWSMNSHPPLSVQTLNTLDRKAFTTALHFAFELSPWVVERAWDARPFASVETMHAAMMAVLDAASTAD
KLALIRAHPELAGKAAIAKQLTAESNAEQASAGLDKLTPDEFARFHALNAAYNTRFGFPFIICVRLNDKASILEAMAARL
SNDEASEIAQAITQIGLISKLRLLDAVTH

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG3195

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=164, Percent_Identity=34.7560975609756, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 20553; Mature: 20421

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPAHKARDDGEGGWSMNSHPPLSVQTLNTLDRKAFTTALHFAFELSPWVVERAWDARPF
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCC
ASVETMHAAMMAVLDAASTADKLALIRAHPELAGKAAIAKQLTAESNAEQASAGLDKLTP
HHHHHHHHHHHHHHHHHCCHHHHHHEECCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCH
DEFARFHALNAAYNTRFGFPFIICVRLNDKASILEAMAARLSNDEASEIAQAITQIGLIS
HHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KLRLLDAVTH
HHHHHHHHCC
>Mature Secondary Structure 
GPAHKARDDGEGGWSMNSHPPLSVQTLNTLDRKAFTTALHFAFELSPWVVERAWDARPF
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCC
ASVETMHAAMMAVLDAASTADKLALIRAHPELAGKAAIAKQLTAESNAEQASAGLDKLTP
HHHHHHHHHHHHHHHHHCCHHHHHHEECCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCH
DEFARFHALNAAYNTRFGFPFIICVRLNDKASILEAMAARLSNDEASEIAQAITQIGLIS
HHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KLRLLDAVTH
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]