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The map label for this gene is gatA [H]

Identifier: 16126712

GI number: 16126712

Start: 2678764

End: 2680257

Strand: Direct

Name: gatA [H]

Synonym: CC_2473

Alternate gene names: 16126712

Gene position: 2678764-2680257 (Clockwise)

Preceding gene: 16126711

Following gene: 16126714

Centisome position: 66.69

GC content: 69.14

Gene sequence:

>1494_bases
ATGACCAAGTTCGCCATCCTGGCAGGCGCGGCCGCTATCGCCCTGCTCGCCGGAAACGCCTGCGCCCAGACGTCCGAAGA
GGCGACCAAGGCGGCCCTGGCCACGATCCAGCAGCGCAACCCCGTCCTGCGCGCCGTGATCGCCACCAACCCGAACGCCC
TGACCGCCGCCAAGGCCCTCGACGCCGAACGCAAGGCCGGCAAGGTCCGCTCGGCCCTGCACGGCGTTCCGATCCTCCTG
AAGGACAATATCGAGAGCGCCGATCCGCTGCCCACCACGGCCGGCTCGCTGGCGCTGAAGGACAACATCACCGGCCGTGA
CGCGCCGGTGGCCAAGCGCCTGCGCGACGCGGGCCTCGTTATCCTGGGCAAGGCCAACCTGTCGGAGTGGGCCAATATCC
GCTCCAACCACTCGATCAGCGGCTGGAGCGCGGTGGGCGGCACGGTGCGCAACCCCTACGCCCTGGACCGCAGCGCCTGC
GGCTCGTCCAGCGGCTCAGGCGCGGCGGTGGCGGCGGGCCTGGCGCCGCTGGCCATCGGCACCGAGACCGACGGCTCGAT
CACCTGCCCCGCGGCGATCAACGGCCTGGTGGGCCTGAAGCCGACCGTGGGCCTCGTTTCGCGCACCCACATCGTGCCCA
TCAGCCACAGCCAGGATACCGCAGGCCCGATGACCCTGACGGTCGAGGACACGGCCAAGGTGCTGACCATCATCGCCGGC
TCCGACCCGACCGATCCGGCGACCAAGGAGGCCGACGCCCGCAAGACCGACTATGCGGCGGGCCTCTCCAAGACCGCGCT
GAAGGGCGTCACGCTGGCCGTGGCCCGGTTCTACACCGGCTACTCCCCCAAGACCGACGCGGTGTTCGAACGGGCGCTTA
AGGAGCTGGAAGCCCAGGGCGCGACCCTGGTCGACGTCAAGGCGTTCGACGAAGGCCCGATCGGCCGCGCCGAGGGCGTG
GTGCTCTACACCGAGCTGAAGGCGGACCTCGCCGCATACCTCGCCTCGACCGATCCCAAGAAGGTGCCGACCCGCACCCT
GGCCGACGTGATCGCCTTCAACAAGGCGACCCCGAAGGAGTTCGAATGGTTCGGCCAGGAAAGCTTCGAGCGGGCCGAGA
AGACCAAGGGCCTGGACGATCCCGAGTATCTGAAGGCTCTGGCCGACTCCAAGCGTCTGGCCGGCCCCGAGGGCATCGAC
AAGATCCTGAAAGACACCGGCGCTGTGGCCATCATCGCCCCGACGACCGGCCCGGCCTGGACCATCGATCCGTTGAACGG
CGACAACTATGGCGGCTCGTCGACCACCCTGCCCGCCGTGGCCGGCTATCCGCACCTGACCGTGCCGATGGGCGACGTAT
CAGGCCTGCCGGTCGGACTGTCGTTCATCGGTCCGGCCTGGAGCGAGAAGCTGCTGCTGAACCTGGGCTACGCCTACGAG
CAGGCGACGAAGCACCGCAAGGCGCCGACCTTCAAGGCGACGATCGCGCCCTAG

Upstream 100 bases:

>100_bases
CACGACTGTGGCGGTTGGCGCTTGGCGAGCGGAGATTTTTGACCCCATGCTCGCGGCCGTCCGGCCGCCGGAGTCGCGGC
CGCGTTTGGGGAAACGCTTC

Downstream 100 bases:

>100_bases
CTGCTGTGATCGGCGATGATGCGCCAGCCGCCGGGCTCCAGGGCCATGATCAGGCTGGTGTATCCCTTGGCCGAGGTCCC
GTTGGGATAGGCCAGCGCCC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTKFAILAGAAAIALLAGNACAQTSEEATKAALATIQQRNPVLRAVIATNPNALTAAKALDAERKAGKVRSALHGVPILL
KDNIESADPLPTTAGSLALKDNITGRDAPVAKRLRDAGLVILGKANLSEWANIRSNHSISGWSAVGGTVRNPYALDRSAC
GSSSGSGAAVAAGLAPLAIGTETDGSITCPAAINGLVGLKPTVGLVSRTHIVPISHSQDTAGPMTLTVEDTAKVLTIIAG
SDPTDPATKEADARKTDYAAGLSKTALKGVTLAVARFYTGYSPKTDAVFERALKELEAQGATLVDVKAFDEGPIGRAEGV
VLYTELKADLAAYLASTDPKKVPTRTLADVIAFNKATPKEFEWFGQESFERAEKTKGLDDPEYLKALADSKRLAGPEGID
KILKDTGAVAIIAPTTGPAWTIDPLNGDNYGGSSTTLPAVAGYPHLTVPMGDVSGLPVGLSFIGPAWSEKLLLNLGYAYE
QATKHRKAPTFKATIAP

Sequences:

>Translated_497_residues
MTKFAILAGAAAIALLAGNACAQTSEEATKAALATIQQRNPVLRAVIATNPNALTAAKALDAERKAGKVRSALHGVPILL
KDNIESADPLPTTAGSLALKDNITGRDAPVAKRLRDAGLVILGKANLSEWANIRSNHSISGWSAVGGTVRNPYALDRSAC
GSSSGSGAAVAAGLAPLAIGTETDGSITCPAAINGLVGLKPTVGLVSRTHIVPISHSQDTAGPMTLTVEDTAKVLTIIAG
SDPTDPATKEADARKTDYAAGLSKTALKGVTLAVARFYTGYSPKTDAVFERALKELEAQGATLVDVKAFDEGPIGRAEGV
VLYTELKADLAAYLASTDPKKVPTRTLADVIAFNKATPKEFEWFGQESFERAEKTKGLDDPEYLKALADSKRLAGPEGID
KILKDTGAVAIIAPTTGPAWTIDPLNGDNYGGSSTTLPAVAGYPHLTVPMGDVSGLPVGLSFIGPAWSEKLLLNLGYAYE
QATKHRKAPTFKATIAP
>Mature_496_residues
TKFAILAGAAAIALLAGNACAQTSEEATKAALATIQQRNPVLRAVIATNPNALTAAKALDAERKAGKVRSALHGVPILLK
DNIESADPLPTTAGSLALKDNITGRDAPVAKRLRDAGLVILGKANLSEWANIRSNHSISGWSAVGGTVRNPYALDRSACG
SSSGSGAAVAAGLAPLAIGTETDGSITCPAAINGLVGLKPTVGLVSRTHIVPISHSQDTAGPMTLTVEDTAKVLTIIAGS
DPTDPATKEADARKTDYAAGLSKTALKGVTLAVARFYTGYSPKTDAVFERALKELEAQGATLVDVKAFDEGPIGRAEGVV
LYTELKADLAAYLASTDPKKVPTRTLADVIAFNKATPKEFEWFGQESFERAEKTKGLDDPEYLKALADSKRLAGPEGIDK
ILKDTGAVAIIAPTTGPAWTIDPLNGDNYGGSSTTLPAVAGYPHLTVPMGDVSGLPVGLSFIGPAWSEKLLLNLGYAYEQ
ATKHRKAPTFKATIAP

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=26.5060240963855, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI195972892, Length=227, Percent_Identity=29.9559471365639, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=457, Percent_Identity=23.8512035010941, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=256, Percent_Identity=28.90625, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=424, Percent_Identity=27.8301886792453, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323950, Length=226, Percent_Identity=31.4159292035398, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648113, Length=488, Percent_Identity=29.0983606557377, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21356731, Length=475, Percent_Identity=25.2631578947368, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=25.1521298174442, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652985, Length=485, Percent_Identity=25.360824742268, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922090, Length=485, Percent_Identity=25.360824742268, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652981, Length=485, Percent_Identity=25.360824742268, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652983, Length=485, Percent_Identity=25.360824742268, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648435, Length=235, Percent_Identity=30.2127659574468, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648437, Length=235, Percent_Identity=30.2127659574468, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550774, Length=235, Percent_Identity=30.2127659574468, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161078093, Length=497, Percent_Identity=25.9557344064386, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648441, Length=179, Percent_Identity=31.2849162011173, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648439, Length=179, Percent_Identity=31.2849162011173, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 51467; Mature: 51336

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFAILAGAAAIALLAGNACAQTSEEATKAALATIQQRNPVLRAVIATNPNALTAAKAL
CCCEEHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
DAERKAGKVRSALHGVPILLKDNIESADPLPTTAGSLALKDNITGRDAPVAKRLRDAGLV
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCEE
ILGKANLSEWANIRSNHSISGWSAVGGTVRNPYALDRSACGSSSGSGAAVAAGLAPLAIG
EEECCCHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCEEEC
TETDGSITCPAAINGLVGLKPTVGLVSRTHIVPISHSQDTAGPMTLTVEDTAKVLTIIAG
CCCCCCEECHHHHCCCCCCCCCHHHHCCEEEEEECCCCCCCCCEEEEEECCCEEEEEEEC
SDPTDPATKEADARKTDYAAGLSKTALKGVTLAVARFYTGYSPKTDAVFERALKELEAQG
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
ATLVDVKAFDEGPIGRAEGVVLYTELKADLAAYLASTDPKKVPTRTLADVIAFNKATPKE
CEEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHH
FEWFGQESFERAEKTKGLDDPEYLKALADSKRLAGPEGIDKILKDTGAVAIIAPTTGPAW
HHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCE
TIDPLNGDNYGGSSTTLPAVAGYPHLTVPMGDVSGLPVGLSFIGPAWSEKLLLNLGYAYE
EECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHCHHHH
QATKHRKAPTFKATIAP
HHHHHCCCCCCEEECCC
>Mature Secondary Structure 
TKFAILAGAAAIALLAGNACAQTSEEATKAALATIQQRNPVLRAVIATNPNALTAAKAL
CCEEHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
DAERKAGKVRSALHGVPILLKDNIESADPLPTTAGSLALKDNITGRDAPVAKRLRDAGLV
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCEE
ILGKANLSEWANIRSNHSISGWSAVGGTVRNPYALDRSACGSSSGSGAAVAAGLAPLAIG
EEECCCHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCEEEC
TETDGSITCPAAINGLVGLKPTVGLVSRTHIVPISHSQDTAGPMTLTVEDTAKVLTIIAG
CCCCCCEECHHHHCCCCCCCCCHHHHCCEEEEEECCCCCCCCCEEEEEECCCEEEEEEEC
SDPTDPATKEADARKTDYAAGLSKTALKGVTLAVARFYTGYSPKTDAVFERALKELEAQG
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
ATLVDVKAFDEGPIGRAEGVVLYTELKADLAAYLASTDPKKVPTRTLADVIAFNKATPKE
CEEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHH
FEWFGQESFERAEKTKGLDDPEYLKALADSKRLAGPEGIDKILKDTGAVAIIAPTTGPAW
HHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCE
TIDPLNGDNYGGSSTTLPAVAGYPHLTVPMGDVSGLPVGLSFIGPAWSEKLLLNLGYAYE
EECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHCHHHH
QATKHRKAPTFKATIAP
HHHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]