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The map label for this gene is pyrB

Identifier: 16126682

GI number: 16126682

Start: 2647627

End: 2648625

Strand: Direct

Name: pyrB

Synonym: CC_2443

Alternate gene names: 16126682

Gene position: 2647627-2648625 (Clockwise)

Preceding gene: 16126681

Following gene: 16126683

Centisome position: 65.91

GC content: 68.77

Gene sequence:

>999_bases
ATGACGGCTTCCGCCCATGACCCCGCCCGCGAGGCGATCGAGACGATCCGCGCCCGCGTCTTCGCCTTTCCCAAGCGCCA
TTTCCTGTCGGCCGGCGATCTGAACGCGCCTCAGGCGGCCGATCTGCTGGATCTGGCCGACGCGTTCGTGGCCCTGAACC
GGCAAACGTCCAAGACCCTGGATATTCTCAAGGGCCGGACGCTGATGAACCTGTTCTTCGAGAACAGCACCCGCACCCAG
AGCTCGTTCGAGCTGGCGGGCAAGCGTCTGGGCGCCGACGTCGTCAACATGAACCCCAAGACCTCGTCGGTCGCCAAGGG
CGAGACCCTGATCGACACGGCCGTCACCCTGAACGCCATGCGGCCCGACCTCTTGGTCGTCCGCCACGCCTCGTCCGGCG
CGGCCAGCCTACTGAGCCAAAAGGTCAGCGGCCACGTCGTCAACGCCGGCGACGGCCAGCACGAGCACCCGACCCAGGCC
CTCCTGGACGCCCTGTCGATCCGACGCGCCTTTGGCCGCGTGTCGGGCCTGACTGTGGCGATCTGCGGCGACGTGCTGCA
CAGCCGCGTGGCGCGTTCGAACGTCGCCTTGCTGCACACCCTGGGCGCCAGCGTGCGTCTGGTGGGACCGCCCACCCTGA
TGCCGGCCCAGGCCGAGCGCTGGGGCGTGGCCGTGCACCACGACATGAAGTCGGGCCTGGCCGGCGCCGACGTCGTCATG
ATGCTGCGCCTGCAGCTGGAGCGCATGCAGGGCGCCTTCGTGCCTTCGACCCGTGAATACTTCCGCTTTTACGGCCTTGA
CCGGGAGAAGCTCTCCCGCGCTGCGCCTGGCGCCAAGGTCATGCACCCAGGCCCGATGAACCGGGGCGTCGAGATCGACT
CCGACGTCGCCGACGATCCGGCCGTAAGCCTGATCCAGGACCAGGTCGAGATGGGCGTCGCCGCCCGGATGGCGGTGCTG
GCGAGCCTGGCGGCCAGGATCGAGGGGGCTGGTCGATGA

Upstream 100 bases:

>100_bases
ACAAGCCAGGGCATGGATTGAGGCGACGATCCGGCCCGTTGTGGGCCACCGCCCAGCGCGGCCTTTCCTTAACGGTCAAA
GCCGCCTAAGACGCGGGCTC

Downstream 100 bases:

>100_bases
AGCGTCCTGCGTCCTTCGAGGCTCGCCTTCGGCTCACACCTCAGGATGAGGTTTTCAGCAGCAACGTTCCTCATCCTGAG
GCGCCCGCAAAGCGGGCCTC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTASAHDPAREAIETIRARVFAFPKRHFLSAGDLNAPQAADLLDLADAFVALNRQTSKTLDILKGRTLMNLFFENSTRTQ
SSFELAGKRLGADVVNMNPKTSSVAKGETLIDTAVTLNAMRPDLLVVRHASSGAASLLSQKVSGHVVNAGDGQHEHPTQA
LLDALSIRRAFGRVSGLTVAICGDVLHSRVARSNVALLHTLGASVRLVGPPTLMPAQAERWGVAVHHDMKSGLAGADVVM
MLRLQLERMQGAFVPSTREYFRFYGLDREKLSRAAPGAKVMHPGPMNRGVEIDSDVADDPAVSLIQDQVEMGVAARMAVL
ASLAARIEGAGR

Sequences:

>Translated_332_residues
MTASAHDPAREAIETIRARVFAFPKRHFLSAGDLNAPQAADLLDLADAFVALNRQTSKTLDILKGRTLMNLFFENSTRTQ
SSFELAGKRLGADVVNMNPKTSSVAKGETLIDTAVTLNAMRPDLLVVRHASSGAASLLSQKVSGHVVNAGDGQHEHPTQA
LLDALSIRRAFGRVSGLTVAICGDVLHSRVARSNVALLHTLGASVRLVGPPTLMPAQAERWGVAVHHDMKSGLAGADVVM
MLRLQLERMQGAFVPSTREYFRFYGLDREKLSRAAPGAKVMHPGPMNRGVEIDSDVADDPAVSLIQDQVEMGVAARMAVL
ASLAARIEGAGR
>Mature_331_residues
TASAHDPAREAIETIRARVFAFPKRHFLSAGDLNAPQAADLLDLADAFVALNRQTSKTLDILKGRTLMNLFFENSTRTQS
SFELAGKRLGADVVNMNPKTSSVAKGETLIDTAVTLNAMRPDLLVVRHASSGAASLLSQKVSGHVVNAGDGQHEHPTQAL
LDALSIRRAFGRVSGLTVAICGDVLHSRVARSNVALLHTLGASVRLVGPPTLMPAQAERWGVAVHHDMKSGLAGADVVMM
LRLQLERMQGAFVPSTREYFRFYGLDREKLSRAAPGAKVMHPGPMNRGVEIDSDVADDPAVSLIQDQVEMGVAARMAVLA
SLAARIEGAGR

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=37.5, Blast_Score=175, Evalue=5e-44,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=35.031847133758, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786469, Length=231, Percent_Identity=27.7056277056277, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790703, Length=233, Percent_Identity=28.3261802575107, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=336, Percent_Identity=32.7380952380952, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=336, Percent_Identity=35.1190476190476, Blast_Score=149, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=34.6278317152104, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_CAUCR (Q9A5K4)

Other databases:

- EMBL:   AE005673
- PIR:   B87552
- RefSeq:   NP_421246.1
- ProteinModelPortal:   Q9A5K4
- SMR:   Q9A5K4
- GeneID:   943703
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_2443
- NMPDR:   fig|190650.1.peg.2426
- TIGR:   CC_2443
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35510; Mature: 35379

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASAHDPAREAIETIRARVFAFPKRHFLSAGDLNAPQAADLLDLADAFVALNRQTSKTL
CCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
DILKGRTLMNLFFENSTRTQSSFELAGKRLGADVVNMNPKTSSVAKGETLIDTAVTLNAM
HHHHCCHHHHHHHCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCHHCCCHHHHHHHHHHCC
RPDLLVVRHASSGAASLLSQKVSGHVVNAGDGQHEHPTQALLDALSIRRAFGRVSGLTVA
CCCEEEEEECCCCHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
ICGDVLHSRVARSNVALLHTLGASVRLVGPPTLMPAQAERWGVAVHHDMKSGLAGADVVM
HHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCCCCHHHCCEEEEHHHHCCCCHHHHHH
MLRLQLERMQGAFVPSTREYFRFYGLDREKLSRAAPGAKVMHPGPMNRGVEIDSDVADDP
HHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCH
AVSLIQDQVEMGVAARMAVLASLAARIEGAGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TASAHDPAREAIETIRARVFAFPKRHFLSAGDLNAPQAADLLDLADAFVALNRQTSKTL
CCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
DILKGRTLMNLFFENSTRTQSSFELAGKRLGADVVNMNPKTSSVAKGETLIDTAVTLNAM
HHHHCCHHHHHHHCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCHHCCCHHHHHHHHHHCC
RPDLLVVRHASSGAASLLSQKVSGHVVNAGDGQHEHPTQALLDALSIRRAFGRVSGLTVA
CCCEEEEEECCCCHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
ICGDVLHSRVARSNVALLHTLGASVRLVGPPTLMPAQAERWGVAVHHDMKSGLAGADVVM
HHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCCCCHHHCCEEEEHHHHCCCCHHHHHH
MLRLQLERMQGAFVPSTREYFRFYGLDREKLSRAAPGAKVMHPGPMNRGVEIDSDVADDP
HHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCH
AVSLIQDQVEMGVAARMAVLASLAARIEGAGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647