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The map label for this gene is virB4 [H]

Identifier: 16126658

GI number: 16126658

Start: 2623303

End: 2625687

Strand: Direct

Name: virB4 [H]

Synonym: CC_2419

Alternate gene names: 16126658

Gene position: 2623303-2625687 (Clockwise)

Preceding gene: 16126657

Following gene: 16126659

Centisome position: 65.31

GC content: 66.04

Gene sequence:

>2385_bases
ATGACCTCGCGCTTCCTCAGCCTGGACCTTGGGGGACGTCTGGCGCCGCGCGAAGCCAGCATTGGCGATCGTCTCCCCTA
TGCGCGGCATATCGATGACCTGACCCTGATCACGCGCGATGGTCAGCTGCTGCAAGTCATCCAGTTGGATGGGCTGGCGT
TCGAGACGTCCGACGATCAGGAACTGACCTACCGCAAACACATGCGGGAGATCTTGCTGCGCAGCGCCGCCAGCTCGCGG
ATCATCATCTATCATCATCTGATCCGCCGGCAGGTCGAGCCCAAGTCCGAGCCTGTACGGAGCGACCCCTTCAGCCAAGC
GCTGGACCAAGCCTGGCGAGACCGGCTGGCCGGTCGGCGACTGTTCGTCAACCAGCTCTATCTGACTCTGGTCAGCCGCC
CGGCGCAGGGCAAGGTCGGCTGGCTGGACCGGCTGGCGCGCGCGGCCCCGGCCGGGGCTGACGCCCAGGCCCAGGACCTA
CGCCGTCTGCACAGTCTGCGCGAGAGCTTGCAAGCGGGTTTGACCGCCTATGGCGCGCGCACCCTTGGCAAGCAGCGCGT
GCGCGGCGTCGAGCGGTCAGAGCCCTGCGCCTTCCTCGCCGAGATCCTCAACGGTGAGCCTAACACGATGGCGACGCCCA
GCGGCGATATCGGTCAGGCGCTTGGCGATCGTCGGCTGACCTTTGGCCTGGACGCTCTGGAGATCGGCGCAGGCGCCAAG
CGCGACCGCCGCTTTGGGGCGATTGTGTCGCTCAAGGACTACCCCGCTCGCTCCGCGCCCGGGATGTTCGATGCGGTGTT
TCGCCTGCCGGCCGAGATGGTGATGACGGAGAGCTTCTCGTTCCTGGATCGCCAGGCTGCGCTCGACCGGATGGGCTTGG
CCCTGCGCCGCTTGCGCGCCGCCGAGGACGATGCGTTCTCGTTGCGCGATGACCTTACCGCCGCGCGCGATGAGGTTGGC
GCGGGCCGTTCAGCGTTTGGCGAACACCACATGTCGGTGCTCGTCAAGGCCGACAGCTTAGCCGAGTTGGACGCCAGCGT
GGCCGACCTGCAAGCCGCGTTCAATGAAGCCGGCGCGGTGGCGGTGCGCGAGGACGTCAATCTGGAGCCGGCGTTCTGGG
CGCAGTTTCCAGGAAACTTCAAGTTCATCGCACGCAAGGCGATGATCTCAGCGGGCAATTTTGCGGCTTTCGCCTCCCTG
CATAACCATTCGCCCGGTAAGGCCGAAGGGGGGCCTTGGGGCCCGCCGGTCAGCCTCCTTGAGACGACCGCGCATGGTCC
CTACCACTTCAATTTCCACAATGGGGATCTTGGCAATTTCACGGTTATCGGCCCCAGCGGGGCGGGTAAAACCGTGCTCC
TGACCTTCCTTTTGGCCCAGGCTCGGCGGTTTGATCCCCAGATTGTCTATTTCGACAAGGACCGCGGGGCCGAGCCCTTC
ATCCGCGCCATCGGCGGCCGCTACGACGTGCTGCGTCCCGGGGAGCCTTCTGGCCTGAATCCGCTGCTGATGGACGACAC
TGCAGCCAACCGCGCTTTTGTCGCCGACTGGCTGGCGCAGTTGCTGGGCGGCGCCGAGCGCCTGGAAGCCGAGGACTTGG
CGATGATCGCAGATGCGGTCGACGCCAATTTTGACCAGCCCCCCGCCAATCGACGCCTGACGTTTCTCCGCGAGCTCTTT
GTCGGCGCCCGCCGTCCTCGCGCCGGCGATCTGGCCGCGCGCCTGCGCCCCTGGTGCGAGGGCGGCGAACACGCCTGGCT
GTTCGACAGCACCGAAGACCTCATGCGCCTAGATGAGCGCGCCTATGGCTTCGACATGACGCGCATTCTCGATGCGCCGA
TCACCCGCACGCCAGCGATGATGTACCTGTTCCACCGAATCGAGCAGGCGCTCGACGGTCGCCCCACCATCATCGTCGTC
GACGAGGGCTGGAAAGCGCTCGACGACAGCATCTTCACCCAGCGGCTGAAGGACTGGGAAAAGACCTTGCGCAAACGCAA
CGGCGTTGTCGGCTTCTGTACGCAGAGCGCCGCCGACGCGCTGGAGAGCCGGATCGCCTCCGCGATCGTCGAACAGGCCG
CCACCCAGATCTTCCTGCCCAACCCGAAAGCCAAGCCGGCCGACTATATCGACGGTTTTGGCCTCACCCCCCACGAGTTT
GACCTCGTGCGCGCCTTGCCCGACACCAGCCGCTGTTTCCTGGTCAAGCAAGCCGACCACAGCGCGGTGGCGCGCCTGGA
TCTCGCCGGCCTGGACGATGTGCTGACGGTCCTGGCCGGCAACGAACGGCGCGTGCGAAGACTAGATCAGGTGCGCGCGG
AGGTCGGCGACGATCCCGCCGCCTGGCTGCCGCCGTACGTCGCCAGCGTGAGCGAGCATCGCTAG

Upstream 100 bases:

>100_bases
GCCTGAACGAGCCGCGCTTCTTCGACCTCTGGATCACCAAGGTCAGCCGCTGTCCGAACATCCGCAATCGCGGGTTCTGG
CAGGCCAACTCCTACCGCCC

Downstream 100 bases:

>100_bases
TGGACGTCTGCCCCGCCCCTCCTCCGACCGCCGGCGTCATCCGGGGCGTCCTGTCCACAATCGACTGCCACACGCGCGTT
TACGCCCAGAGCGGCTATGA

Product: type IV secretion system protein B4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 794; Mature: 793

Protein sequence:

>794_residues
MTSRFLSLDLGGRLAPREASIGDRLPYARHIDDLTLITRDGQLLQVIQLDGLAFETSDDQELTYRKHMREILLRSAASSR
IIIYHHLIRRQVEPKSEPVRSDPFSQALDQAWRDRLAGRRLFVNQLYLTLVSRPAQGKVGWLDRLARAAPAGADAQAQDL
RRLHSLRESLQAGLTAYGARTLGKQRVRGVERSEPCAFLAEILNGEPNTMATPSGDIGQALGDRRLTFGLDALEIGAGAK
RDRRFGAIVSLKDYPARSAPGMFDAVFRLPAEMVMTESFSFLDRQAALDRMGLALRRLRAAEDDAFSLRDDLTAARDEVG
AGRSAFGEHHMSVLVKADSLAELDASVADLQAAFNEAGAVAVREDVNLEPAFWAQFPGNFKFIARKAMISAGNFAAFASL
HNHSPGKAEGGPWGPPVSLLETTAHGPYHFNFHNGDLGNFTVIGPSGAGKTVLLTFLLAQARRFDPQIVYFDKDRGAEPF
IRAIGGRYDVLRPGEPSGLNPLLMDDTAANRAFVADWLAQLLGGAERLEAEDLAMIADAVDANFDQPPANRRLTFLRELF
VGARRPRAGDLAARLRPWCEGGEHAWLFDSTEDLMRLDERAYGFDMTRILDAPITRTPAMMYLFHRIEQALDGRPTIIVV
DEGWKALDDSIFTQRLKDWEKTLRKRNGVVGFCTQSAADALESRIASAIVEQAATQIFLPNPKAKPADYIDGFGLTPHEF
DLVRALPDTSRCFLVKQADHSAVARLDLAGLDDVLTVLAGNERRVRRLDQVRAEVGDDPAAWLPPYVASVSEHR

Sequences:

>Translated_794_residues
MTSRFLSLDLGGRLAPREASIGDRLPYARHIDDLTLITRDGQLLQVIQLDGLAFETSDDQELTYRKHMREILLRSAASSR
IIIYHHLIRRQVEPKSEPVRSDPFSQALDQAWRDRLAGRRLFVNQLYLTLVSRPAQGKVGWLDRLARAAPAGADAQAQDL
RRLHSLRESLQAGLTAYGARTLGKQRVRGVERSEPCAFLAEILNGEPNTMATPSGDIGQALGDRRLTFGLDALEIGAGAK
RDRRFGAIVSLKDYPARSAPGMFDAVFRLPAEMVMTESFSFLDRQAALDRMGLALRRLRAAEDDAFSLRDDLTAARDEVG
AGRSAFGEHHMSVLVKADSLAELDASVADLQAAFNEAGAVAVREDVNLEPAFWAQFPGNFKFIARKAMISAGNFAAFASL
HNHSPGKAEGGPWGPPVSLLETTAHGPYHFNFHNGDLGNFTVIGPSGAGKTVLLTFLLAQARRFDPQIVYFDKDRGAEPF
IRAIGGRYDVLRPGEPSGLNPLLMDDTAANRAFVADWLAQLLGGAERLEAEDLAMIADAVDANFDQPPANRRLTFLRELF
VGARRPRAGDLAARLRPWCEGGEHAWLFDSTEDLMRLDERAYGFDMTRILDAPITRTPAMMYLFHRIEQALDGRPTIIVV
DEGWKALDDSIFTQRLKDWEKTLRKRNGVVGFCTQSAADALESRIASAIVEQAATQIFLPNPKAKPADYIDGFGLTPHEF
DLVRALPDTSRCFLVKQADHSAVARLDLAGLDDVLTVLAGNERRVRRLDQVRAEVGDDPAAWLPPYVASVSEHR
>Mature_793_residues
TSRFLSLDLGGRLAPREASIGDRLPYARHIDDLTLITRDGQLLQVIQLDGLAFETSDDQELTYRKHMREILLRSAASSRI
IIYHHLIRRQVEPKSEPVRSDPFSQALDQAWRDRLAGRRLFVNQLYLTLVSRPAQGKVGWLDRLARAAPAGADAQAQDLR
RLHSLRESLQAGLTAYGARTLGKQRVRGVERSEPCAFLAEILNGEPNTMATPSGDIGQALGDRRLTFGLDALEIGAGAKR
DRRFGAIVSLKDYPARSAPGMFDAVFRLPAEMVMTESFSFLDRQAALDRMGLALRRLRAAEDDAFSLRDDLTAARDEVGA
GRSAFGEHHMSVLVKADSLAELDASVADLQAAFNEAGAVAVREDVNLEPAFWAQFPGNFKFIARKAMISAGNFAAFASLH
NHSPGKAEGGPWGPPVSLLETTAHGPYHFNFHNGDLGNFTVIGPSGAGKTVLLTFLLAQARRFDPQIVYFDKDRGAEPFI
RAIGGRYDVLRPGEPSGLNPLLMDDTAANRAFVADWLAQLLGGAERLEAEDLAMIADAVDANFDQPPANRRLTFLRELFV
GARRPRAGDLAARLRPWCEGGEHAWLFDSTEDLMRLDERAYGFDMTRILDAPITRTPAMMYLFHRIEQALDGRPTIIVVD
EGWKALDDSIFTQRLKDWEKTLRKRNGVVGFCTQSAADALESRIASAIVEQAATQIFLPNPKAKPADYIDGFGLTPHEFD
LVRALPDTSRCFLVKQADHSAVARLDLAGLDDVLTVLAGNERRVRRLDQVRAEVGDDPAAWLPPYVASVSEHR

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 87660; Mature: 87529

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRFLSLDLGGRLAPREASIGDRLPYARHIDDLTLITRDGQLLQVIQLDGLAFETSDDQ
CCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCEEEEEEECCEEEECCCCH
ELTYRKHMREILLRSAASSRIIIYHHLIRRQVEPKSEPVRSDPFSQALDQAWRDRLAGRR
HHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHH
LFVNQLYLTLVSRPAQGKVGWLDRLARAAPAGADAQAQDLRRLHSLRESLQAGLTAYGAR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGKQRVRGVERSEPCAFLAEILNGEPNTMATPSGDIGQALGDRRLTFGLDALEIGAGAK
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEECCCHHHHCCCCC
RDRRFGAIVSLKDYPARSAPGMFDAVFRLPAEMVMTESFSFLDRQAALDRMGLALRRLRA
CCCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEDDAFSLRDDLTAARDEVGAGRSAFGEHHMSVLVKADSLAELDASVADLQAAFNEAGAV
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHCCCCE
AVREDVNLEPAFWAQFPGNFKFIARKAMISAGNFAAFASLHNHSPGKAEGGPWGPPVSLL
EEECCCCCCCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
ETTAHGPYHFNFHNGDLGNFTVIGPSGAGKTVLLTFLLAQARRFDPQIVYFDKDRGAEPF
HHCCCCCEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
IRAIGGRYDVLRPGEPSGLNPLLMDDTAANRAFVADWLAQLLGGAERLEAEDLAMIADAV
HHHHCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCHHHCCHHHHHHHHHHH
DANFDQPPANRRLTFLRELFVGARRPRAGDLAARLRPWCEGGEHAWLFDSTEDLMRLDER
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHH
AYGFDMTRILDAPITRTPAMMYLFHRIEQALDGRPTIIVVDEGWKALDDSIFTQRLKDWE
HCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
KTLRKRNGVVGFCTQSAADALESRIASAIVEQAATQIFLPNPKAKPADYIDGFGLTPHEF
HHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHCCCCCCCCHH
DLVRALPDTSRCFLVKQADHSAVARLDLAGLDDVLTVLAGNERRVRRLDQVRAEVGDDPA
HHHHHCCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
AWLPPYVASVSEHR
HHCCHHHHHHCCCC
>Mature Secondary Structure 
TSRFLSLDLGGRLAPREASIGDRLPYARHIDDLTLITRDGQLLQVIQLDGLAFETSDDQ
CCCEEEECCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCEEEEEEECCEEEECCCCH
ELTYRKHMREILLRSAASSRIIIYHHLIRRQVEPKSEPVRSDPFSQALDQAWRDRLAGRR
HHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHH
LFVNQLYLTLVSRPAQGKVGWLDRLARAAPAGADAQAQDLRRLHSLRESLQAGLTAYGAR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGKQRVRGVERSEPCAFLAEILNGEPNTMATPSGDIGQALGDRRLTFGLDALEIGAGAK
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEECCCHHHHCCCCC
RDRRFGAIVSLKDYPARSAPGMFDAVFRLPAEMVMTESFSFLDRQAALDRMGLALRRLRA
CCCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEDDAFSLRDDLTAARDEVGAGRSAFGEHHMSVLVKADSLAELDASVADLQAAFNEAGAV
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHCCCCE
AVREDVNLEPAFWAQFPGNFKFIARKAMISAGNFAAFASLHNHSPGKAEGGPWGPPVSLL
EEECCCCCCCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
ETTAHGPYHFNFHNGDLGNFTVIGPSGAGKTVLLTFLLAQARRFDPQIVYFDKDRGAEPF
HHCCCCCEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
IRAIGGRYDVLRPGEPSGLNPLLMDDTAANRAFVADWLAQLLGGAERLEAEDLAMIADAV
HHHHCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCHHHCCHHHHHHHHHHH
DANFDQPPANRRLTFLRELFVGARRPRAGDLAARLRPWCEGGEHAWLFDSTEDLMRLDER
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHH
AYGFDMTRILDAPITRTPAMMYLFHRIEQALDGRPTIIVVDEGWKALDDSIFTQRLKDWE
HCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
KTLRKRNGVVGFCTQSAADALESRIASAIVEQAATQIFLPNPKAKPADYIDGFGLTPHEF
HHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHCCCCCCCCHH
DLVRALPDTSRCFLVKQADHSAVARLDLAGLDDVLTVLAGNERRVRRLDQVRAEVGDDPA
HHHHHCCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
AWLPPYVASVSEHR
HHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]