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The map label for this gene is vanB [H]

Identifier: 16126633

GI number: 16126633

Start: 2596002

End: 2596952

Strand: Direct

Name: vanB [H]

Synonym: CC_2394

Alternate gene names: 16126633

Gene position: 2596002-2596952 (Clockwise)

Preceding gene: 16126632

Following gene: 16126643

Centisome position: 64.63

GC content: 69.93

Gene sequence:

>951_bases
ATGATCAAGGTTCGGGTGGCGTCCCGCGCCCGGGCGGCGGAAGGCATTGCGCAGCTCGACCTGGTTTGCGCCGAGGGCCG
CGATCTGCCGGCCTTCGAGGCCGGCGCGCATATCGACCTGTTCCTGGGGAACGGCCTGACGCGGCAGTACTCCCTGTGCA
GTGACCCCGCAGAACGCAGCTTCTATCGGATCGCCGTCCTGCGCGAGCTCACCTCGCGTGGCGGATCGGCCTATGTTCAT
GAGGCGCTGACCGAGGGCGCGACGCTGGAGATCAGCCCGCCCCGCAACCTGTTCGCTCTGGACGAAACCGCCGAGGAGCA
TCTGCTGTTCGCGGGCGGGGTCGGCGTGACCCCCATCCTGGCCATGGCCAAACGCCTGAGCGCCCTGGGCCGCGCGTTCA
CCTTCCACTATTGCGCGCGCGATCGGTCTCGGGCCGCATTCCTCAACGAGCTTTCGACCGCGCCCTTCACCAGCGCAGTG
CGTTTGTCGTTCGACGCCGAGCCGGACACCCGTCTGGATCTCGACGTCGCGCTGGCCCGGCCCAAGCCGGGACGCCGCCT
CTATGTCTGCGGCCCCGGCGGGTTCATGACCCACGTCATCGACGGCGCCCTGGCGCGGGGATGGTCTGGGGATCAGATCC
GCAAGGAGCACTTCGCGGCCGCGCCGCTCTCCGCCGGCCAGGACCAGCCGTTCGAGCTGGTGATCGCCAGCTCCGGCCAG
ATCGTGACCGTCGCCGCCGACCAGACCGCCGCGCAAGCCCTGGAGGCGGCGGGCGTCTTCGTCCCCGTCTCCTGCGAGCA
AGGGGTCTGCGGCACGTGCCTGACTCGCGTGATCGACGGCCAGCCCGATCACCGCGACGCCTTCCAGACCGACGAGGAGC
GGGCCGCGAACGACCAGTTCACGCCCTGCTGCTCGCGCGCCCGCTCGCCGCGCCTGGTGCTGGATCTTTAA

Upstream 100 bases:

>100_bases
GTAAGCTGCTGAAGCTGAACATCGACGCCGGCGGCGTGCGTTCGCGCATGGCCATCGACAAGGCGATCGCCGCCGAGACC
GCCTTGGCGGCGCAAGCGAA

Downstream 100 bases:

>100_bases
ACGACGAAGGCCCCGCGTGCAGGTTCGCGCGGGGCTCGGCGCCGGCTATCAGGACGTCGCGTGGTCCTCGTCGACCCGTC
GCGCGAAGGCCTTCATCTCC

Product: vanillate O-demethylase, reductase subunit

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIKVRVASRARAAEGIAQLDLVCAEGRDLPAFEAGAHIDLFLGNGLTRQYSLCSDPAERSFYRIAVLRELTSRGGSAYVH
EALTEGATLEISPPRNLFALDETAEEHLLFAGGVGVTPILAMAKRLSALGRAFTFHYCARDRSRAAFLNELSTAPFTSAV
RLSFDAEPDTRLDLDVALARPKPGRRLYVCGPGGFMTHVIDGALARGWSGDQIRKEHFAAAPLSAGQDQPFELVIASSGQ
IVTVAADQTAAQALEAAGVFVPVSCEQGVCGTCLTRVIDGQPDHRDAFQTDEERAANDQFTPCCSRARSPRLVLDL

Sequences:

>Translated_316_residues
MIKVRVASRARAAEGIAQLDLVCAEGRDLPAFEAGAHIDLFLGNGLTRQYSLCSDPAERSFYRIAVLRELTSRGGSAYVH
EALTEGATLEISPPRNLFALDETAEEHLLFAGGVGVTPILAMAKRLSALGRAFTFHYCARDRSRAAFLNELSTAPFTSAV
RLSFDAEPDTRLDLDVALARPKPGRRLYVCGPGGFMTHVIDGALARGWSGDQIRKEHFAAAPLSAGQDQPFELVIASSGQ
IVTVAADQTAAQALEAAGVFVPVSCEQGVCGTCLTRVIDGQPDHRDAFQTDEERAANDQFTPCCSRARSPRLVLDL
>Mature_316_residues
MIKVRVASRARAAEGIAQLDLVCAEGRDLPAFEAGAHIDLFLGNGLTRQYSLCSDPAERSFYRIAVLRELTSRGGSAYVH
EALTEGATLEISPPRNLFALDETAEEHLLFAGGVGVTPILAMAKRLSALGRAFTFHYCARDRSRAAFLNELSTAPFTSAV
RLSFDAEPDTRLDLDVALARPKPGRRLYVCGPGGFMTHVIDGALARGWSGDQIRKEHFAAAPLSAGQDQPFELVIASSGQ
IVTVAADQTAAQALEAAGVFVPVSCEQGVCGTCLTRVIDGQPDHRDAFQTDEERAANDQFTPCCSRARSPRLVLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=320, Percent_Identity=37.8125, Blast_Score=206, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 33948; Mature: 33948

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVRVASRARAAEGIAQLDLVCAEGRDLPAFEAGAHIDLFLGNGLTRQYSLCSDPAERS
CEEEEECHHHHHHHCHHHEEEEEECCCCCCCCCCCCEEEEEECCCCCEEHHHCCCHHHHH
FYRIAVLRELTSRGGSAYVHEALTEGATLEISPPRNLFALDETAEEHLLFAGGVGVTPIL
HHHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCEEEECCCCCCCEEEECCCCHHHHH
AMAKRLSALGRAFTFHYCARDRSRAAFLNELSTAPFTSAVRLSFDAEPDTRLDLDVALAR
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEEEC
PKPGRRLYVCGPGGFMTHVIDGALARGWSGDQIRKEHFAAAPLSAGQDQPFELVIASSGQ
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCC
IVTVAADQTAAQALEAAGVFVPVSCEQGVCGTCLTRVIDGQPDHRDAFQTDEERAANDQF
EEEEECCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCC
TPCCSRARSPRLVLDL
CHHHHHCCCCEEEEEC
>Mature Secondary Structure
MIKVRVASRARAAEGIAQLDLVCAEGRDLPAFEAGAHIDLFLGNGLTRQYSLCSDPAERS
CEEEEECHHHHHHHCHHHEEEEEECCCCCCCCCCCCEEEEEECCCCCEEHHHCCCHHHHH
FYRIAVLRELTSRGGSAYVHEALTEGATLEISPPRNLFALDETAEEHLLFAGGVGVTPIL
HHHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCEEEECCCCCCCEEEECCCCHHHHH
AMAKRLSALGRAFTFHYCARDRSRAAFLNELSTAPFTSAVRLSFDAEPDTRLDLDVALAR
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEEEC
PKPGRRLYVCGPGGFMTHVIDGALARGWSGDQIRKEHFAAAPLSAGQDQPFELVIASSGQ
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCC
IVTVAADQTAAQALEAAGVFVPVSCEQGVCGTCLTRVIDGQPDHRDAFQTDEERAANDQF
EEEEECCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCC
TPCCSRARSPRLVLDL
CHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098058 [H]