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The map label for this gene is metZ [H]

Identifier: 16126477

GI number: 16126477

Start: 2437525

End: 2438739

Strand: Direct

Name: metZ [H]

Synonym: CC_2238

Alternate gene names: 16126477

Gene position: 2437525-2438739 (Clockwise)

Preceding gene: 16126467

Following gene: 16126478

Centisome position: 60.68

GC content: 67.49

Gene sequence:

>1215_bases
ATGCGCGCGTTTTGCGTAGGAGGCGGGCCCGTGGCCGAGAACCCGAAGGACTGGGACGTCGCGACGAAATTGATCCGTGG
CGGGATCGCGCGCTCTCAGTTCATGGAGACCGCCGAGGCGCTCTATCTGACCCAGGGCTTCACCTACGACAGCGCCGAGG
GCGCCGACCGCCGTTTCGCCGGCGAAGATCCCGGGTTCGTCTATTCGCGGTTCAACAATCCGACCGTGAAGATGTTCGAG
GATCGCCTCGCCCTGCTGGAAGGCGCCGAGGTCTGCCGCGCCCAGGCCACCGGCATGGCCTCGATTCACGCCGCCCTGAT
GGGCCTGGTTCGCGCCGGCGACCATGTCGTGGCCGGTCGCGCGCTCTTTGGCTCGTGCCGCTGGATCGTCAGCGAGTGGC
TGCCGCGCTTCGGTGTGGAGACCACCTTTGTCGACGCCACCGATCCCAAGGCCTGGGAAGCGGCGATGCGGCCCAACACC
AAGGCGGTTCTGGTCGAAACGCCCTCGAACCCGGTGCTGGAGATCACCGACATCCGCGCGGTGTCGGAACTGGCCCACGC
GGTCGGGGCCAAGGTCATCGTCGACAACGTGTTCGCCACCCCGATCTTCCAGAAGCCGCTGGAGCTGGGCGCGGACGTCG
TCGTCTATTCGGCCACCAAGCATATCGACGGCCAGGGCCGCGTGCTGGGCGGGGCCATCCTGACCAGCGAGGCGATCAAC
GAGGAGTTCTACCGCGACAGCCTGCGCCACACCGGCCCGTCGCTGTCGCCGTTCAACGCCTGGGTGATGCTGAAGGGCCT
CGAGACCCTGGATCTGCGGGTGCGCCGCCAGGCCGACAGCGCTTTCGCCCTGGCGAACACCATCGCCGAGCACAAGAAGG
TGCAGACGGTGCTCTATCCGTTCCGCCCCGACCACCCCGGCCACAACGTCGCCAAGGCGCAGATGACCGGCGGCGGCACC
GTGATCGCGCTGGACCTGGGCAGCCGCGAGGCCGCCTTCAAGTTCCTGAATGCGCTGGAGATCGTCGACATCTCCAACAA
TCTGGGCGACGCCAAGTCGATGGCCACGCACCCGCCGACCACCACCCACCGCTCGGTCCCCGAAGCCGAACGCCCGTCAC
TGGGCGTCACCGAAGGCGGCGTGCGCCTGTCGGTGGGCCTGGAGAGCGTGGAAGATCTCAAGCGGGATGTGATCCGTGCG
CTCGATCAGGCGTGA

Upstream 100 bases:

>100_bases
CGAATGGCGCTCGCCTGTGACACCGCACTTTTCCCCTATGACAATTCTCTGACGCTCTGGCAGGCGTTCTGGCTTGACCC
CGCCCCCTCCCCCGCTCCTT

Downstream 100 bases:

>100_bases
GACCTCCCCGCCCATGCAACGCATGAGACGCCGCCGGGGCAGCGACAGCGCCGCCTATGAGGCCCGCACGCGCGACATCG
TCGTGCGCGTCTTCCCCACC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MRAFCVGGGPVAENPKDWDVATKLIRGGIARSQFMETAEALYLTQGFTYDSAEGADRRFAGEDPGFVYSRFNNPTVKMFE
DRLALLEGAEVCRAQATGMASIHAALMGLVRAGDHVVAGRALFGSCRWIVSEWLPRFGVETTFVDATDPKAWEAAMRPNT
KAVLVETPSNPVLEITDIRAVSELAHAVGAKVIVDNVFATPIFQKPLELGADVVVYSATKHIDGQGRVLGGAILTSEAIN
EEFYRDSLRHTGPSLSPFNAWVMLKGLETLDLRVRRQADSAFALANTIAEHKKVQTVLYPFRPDHPGHNVAKAQMTGGGT
VIALDLGSREAAFKFLNALEIVDISNNLGDAKSMATHPPTTTHRSVPEAERPSLGVTEGGVRLSVGLESVEDLKRDVIRA
LDQA

Sequences:

>Translated_404_residues
MRAFCVGGGPVAENPKDWDVATKLIRGGIARSQFMETAEALYLTQGFTYDSAEGADRRFAGEDPGFVYSRFNNPTVKMFE
DRLALLEGAEVCRAQATGMASIHAALMGLVRAGDHVVAGRALFGSCRWIVSEWLPRFGVETTFVDATDPKAWEAAMRPNT
KAVLVETPSNPVLEITDIRAVSELAHAVGAKVIVDNVFATPIFQKPLELGADVVVYSATKHIDGQGRVLGGAILTSEAIN
EEFYRDSLRHTGPSLSPFNAWVMLKGLETLDLRVRRQADSAFALANTIAEHKKVQTVLYPFRPDHPGHNVAKAQMTGGGT
VIALDLGSREAAFKFLNALEIVDISNNLGDAKSMATHPPTTTHRSVPEAERPSLGVTEGGVRLSVGLESVEDLKRDVIRA
LDQA
>Mature_404_residues
MRAFCVGGGPVAENPKDWDVATKLIRGGIARSQFMETAEALYLTQGFTYDSAEGADRRFAGEDPGFVYSRFNNPTVKMFE
DRLALLEGAEVCRAQATGMASIHAALMGLVRAGDHVVAGRALFGSCRWIVSEWLPRFGVETTFVDATDPKAWEAAMRPNT
KAVLVETPSNPVLEITDIRAVSELAHAVGAKVIVDNVFATPIFQKPLELGADVVVYSATKHIDGQGRVLGGAILTSEAIN
EEFYRDSLRHTGPSLSPFNAWVMLKGLETLDLRVRRQADSAFALANTIAEHKKVQTVLYPFRPDHPGHNVAKAQMTGGGT
VIALDLGSREAAFKFLNALEIVDISNNLGDAKSMATHPPTTTHRSVPEAERPSLGVTEGGVRLSVGLESVEDLKRDVIRA
LDQA

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=332, Percent_Identity=35.5421686746988, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI299473759, Length=350, Percent_Identity=34.5714285714286, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI25453487, Length=349, Percent_Identity=31.5186246418338, Blast_Score=152, Evalue=7e-37,
Organism=Escherichia coli, GI1790375, Length=334, Percent_Identity=32.6347305389222, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1789383, Length=389, Percent_Identity=24.1645244215938, Blast_Score=121, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17538055, Length=335, Percent_Identity=38.5074626865672, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17560056, Length=335, Percent_Identity=38.5074626865672, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI71980708, Length=343, Percent_Identity=33.8192419825073, Blast_Score=197, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=347, Percent_Identity=36.3112391930836, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=409, Percent_Identity=32.2738386308068, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=26.2254901960784, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=292, Percent_Identity=28.4246575342466, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=397, Percent_Identity=26.448362720403, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922554, Length=392, Percent_Identity=34.1836734693878, Blast_Score=228, Evalue=7e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43681; Mature: 43681

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAFCVGGGPVAENPKDWDVATKLIRGGIARSQFMETAEALYLTQGFTYDSAEGADRRFA
CEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GEDPGFVYSRFNNPTVKMFEDRLALLEGAEVCRAQATGMASIHAALMGLVRAGDHVVAGR
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEECH
ALFGSCRWIVSEWLPRFGVETTFVDATDPKAWEAAMRPNTKAVLVETPSNPVLEITDIRA
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCEEEEHHHH
VSELAHAVGAKVIVDNVFATPIFQKPLELGADVVVYSATKHIDGQGRVLGGAILTSEAIN
HHHHHHHHCCHHEEHHHHHCHHHHHHHHCCCCEEEEEEEECCCCCCEEECHHHHHHHHHH
EEFYRDSLRHTGPSLSPFNAWVMLKGLETLDLRVRRQADSAFALANTIAEHKKVQTVLYP
HHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEEC
FRPDHPGHNVAKAQMTGGGTVIALDLGSREAAFKFLNALEIVDISNNLGDAKSMATHPPT
CCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHEEEECCCCCCCHHHCCCCCCC
TTHRSVPEAERPSLGVTEGGVRLSVGLESVEDLKRDVIRALDQA
CCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRAFCVGGGPVAENPKDWDVATKLIRGGIARSQFMETAEALYLTQGFTYDSAEGADRRFA
CEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GEDPGFVYSRFNNPTVKMFEDRLALLEGAEVCRAQATGMASIHAALMGLVRAGDHVVAGR
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEECH
ALFGSCRWIVSEWLPRFGVETTFVDATDPKAWEAAMRPNTKAVLVETPSNPVLEITDIRA
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCEEEEHHHH
VSELAHAVGAKVIVDNVFATPIFQKPLELGADVVVYSATKHIDGQGRVLGGAILTSEAIN
HHHHHHHHCCHHEEHHHHHCHHHHHHHHCCCCEEEEEEEECCCCCCEEECHHHHHHHHHH
EEFYRDSLRHTGPSLSPFNAWVMLKGLETLDLRVRRQADSAFALANTIAEHKKVQTVLYP
HHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEEC
FRPDHPGHNVAKAQMTGGGTVIALDLGSREAAFKFLNALEIVDISNNLGDAKSMATHPPT
CCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHEEEECCCCCCCHHHCCCCCCC
TTHRSVPEAERPSLGVTEGGVRLSVGLESVEDLKRDVIRALDQA
CCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]