Click here to switch to the map view.

The map label for this gene is gluQ [H]

Identifier: 16126444

GI number: 16126444

Start: 2410400

End: 2411239

Strand: Direct

Name: gluQ [H]

Synonym: CC_2205

Alternate gene names: 16126444

Gene position: 2410400-2411239 (Clockwise)

Preceding gene: 16126443

Following gene: 16126450

Centisome position: 60.01

GC content: 68.81

Gene sequence:

>840_bases
TTGACGTTCGTCACCCGGTTCGCGCCTTCCCCCACGGGTTACCTGCATCGCGGCCACGCCTATTCGGCGTTGACCGCCTT
CGAGGCGGCGCGCGTGACGGGCGGGCGCTTCCTTCTTCGCATCGAGGACATCGACGCCACCCGTTGCCGGCCGGAGTACG
AGGCGGCGATCCTTGAGGATCTCGCCTGGCTGGGCCTGAACTGGGAAGCGCCCGTGCGCCGACAGTCCGAGCACCTGGCC
GACTACGAGGCGGCCATCGCGGGCCTGCGCGACCGGGGTCTGGTCTATCGCTGCTTCAAGACCCGCAAGGAAATCGACAT
TGGGCGCGCGCCGCACGAGCCCGCTATCGCCTATGTCGGCGCACCGCTCGGCGCTGACGAGGAAGCCCAACGGCTGGCGC
GCGGCGAGCCCTTCGCCTGGCGGCTGTCTCTGGCCGCAGCACGAGAGGCCCTCGGCGGCTTTGATCACCTCAGTTTCCTG
GAGGAAGGCGCAGACGGATCGTCACCGCGCACCGTCCAAGCCCTGCCCGAAACTGCGGGCGACATCGTCCTGGCCCGCAA
GGATGTCGGCGTGGCCTACCATCTGGCGGTCGTCCATGACGACGCGCTGCAAGGGGTCACCCATGTGACCCGGGGCGAGG
ACCTGTTCGAGGCCACCCACATCCAGCGTCTGCTGCAGGCCCTGCTGGGCCTGCCGACCCCGATCTATCGCCACCACGGC
CTGCTGACCGGGCCTGACGGCAAGCGCTACGCCAAGCGCGACAAGGCCCAGACCTTGCGGGAGCTGCGGGCGTCGGGCGT
GAGCGCACAAGATCTTCGGGCGGAACTCGGCTTTTCCTAG

Upstream 100 bases:

>100_bases
TCGCCCCGCTCAAGGACCTGCTCGGCGGCGAGGAAGCCTTCCTGCGCGCCGTCTTCGAGCACCTGGGCATGTTCGCCAAG
GTCATCGACCAGGATCCGGC

Downstream 100 bases:

>100_bases
AACCCCGATCCTCCCGGCGAACGCCGGGACCCAGATCGAAGCCGCCGACGTCTTTCCGCTCAGCCCAAAGAGATGTCGCA
CCATCCCTGACCGCCCCGGG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTFVTRFAPSPTGYLHRGHAYSALTAFEAARVTGGRFLLRIEDIDATRCRPEYEAAILEDLAWLGLNWEAPVRRQSEHLA
DYEAAIAGLRDRGLVYRCFKTRKEIDIGRAPHEPAIAYVGAPLGADEEAQRLARGEPFAWRLSLAAAREALGGFDHLSFL
EEGADGSSPRTVQALPETAGDIVLARKDVGVAYHLAVVHDDALQGVTHVTRGEDLFEATHIQRLLQALLGLPTPIYRHHG
LLTGPDGKRYAKRDKAQTLRELRASGVSAQDLRAELGFS

Sequences:

>Translated_279_residues
MTFVTRFAPSPTGYLHRGHAYSALTAFEAARVTGGRFLLRIEDIDATRCRPEYEAAILEDLAWLGLNWEAPVRRQSEHLA
DYEAAIAGLRDRGLVYRCFKTRKEIDIGRAPHEPAIAYVGAPLGADEEAQRLARGEPFAWRLSLAAAREALGGFDHLSFL
EEGADGSSPRTVQALPETAGDIVLARKDVGVAYHLAVVHDDALQGVTHVTRGEDLFEATHIQRLLQALLGLPTPIYRHHG
LLTGPDGKRYAKRDKAQTLRELRASGVSAQDLRAELGFS
>Mature_278_residues
TFVTRFAPSPTGYLHRGHAYSALTAFEAARVTGGRFLLRIEDIDATRCRPEYEAAILEDLAWLGLNWEAPVRRQSEHLAD
YEAAIAGLRDRGLVYRCFKTRKEIDIGRAPHEPAIAYVGAPLGADEEAQRLARGEPFAWRLSLAAAREALGGFDHLSFLE
EGADGSSPRTVQALPETAGDIVLARKDVGVAYHLAVVHDDALQGVTHVTRGEDLFEATHIQRLLQALLGLPTPIYRHHGL
LTGPDGKRYAKRDKAQTLRELRASGVSAQDLRAELGFS

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=275, Percent_Identity=30.9090909090909, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1788743, Length=269, Percent_Identity=33.457249070632, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI226510925, Length=265, Percent_Identity=35.4716981132075, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1786895, Length=95, Percent_Identity=37.8947368421053, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=269, Percent_Identity=29.7397769516729, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324540, Length=270, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24665321, Length=281, Percent_Identity=34.8754448398576, Blast_Score=127, Evalue=9e-30,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 30670; Mature: 30539

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFVTRFAPSPTGYLHRGHAYSALTAFEAARVTGGRFLLRIEDIDATRCRPEYEAAILED
CCEEECCCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
LAWLGLNWEAPVRRQSEHLADYEAAIAGLRDRGLVYRCFKTRKEIDIGRAPHEPAIAYVG
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEC
APLGADEEAQRLARGEPFAWRLSLAAAREALGGFDHLSFLEEGADGSSPRTVQALPETAG
CCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEHHCCCCCC
DIVLARKDVGVAYHLAVVHDDALQGVTHVTRGEDLFEATHIQRLLQALLGLPTPIYRHHG
CEEEEEECCCEEEEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHCC
LLTGPDGKRYAKRDKAQTLRELRASGVSAQDLRAELGFS
CEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC
>Mature Secondary Structure 
TFVTRFAPSPTGYLHRGHAYSALTAFEAARVTGGRFLLRIEDIDATRCRPEYEAAILED
CEEECCCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
LAWLGLNWEAPVRRQSEHLADYEAAIAGLRDRGLVYRCFKTRKEIDIGRAPHEPAIAYVG
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEC
APLGADEEAQRLARGEPFAWRLSLAAAREALGGFDHLSFLEEGADGSSPRTVQALPETAG
CCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEHHCCCCCC
DIVLARKDVGVAYHLAVVHDDALQGVTHVTRGEDLFEATHIQRLLQALLGLPTPIYRHHG
CEEEEEECCCEEEEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHCC
LLTGPDGKRYAKRDKAQTLRELRASGVSAQDLRAELGFS
CEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA