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The map label for this gene is dacF [H]

Identifier: 16126400

GI number: 16126400

Start: 2365881

End: 2367188

Strand: Direct

Name: dacF [H]

Synonym: CC_2161

Alternate gene names: 16126400

Gene position: 2365881-2367188 (Clockwise)

Preceding gene: 16126394

Following gene: 16126402

Centisome position: 58.9

GC content: 67.81

Gene sequence:

>1308_bases
ATGTTCGCCAGGCTTACCTCCGCCCGCTCGTTGCTCGCCGCCGCTGGTCTGGCCCTGACGTGTCTTGCCGGTACGCTCGC
GCCATCCGCCGCCTCGGCCGCCGTCCCTTATCTGCAACTGAACGCATCAGAGCCGAAGTACGCGGCCATCGTGATCGACG
CCAATTCGGGCGAGGTCCTCTACGACAAGCGCGGTGACAGCCCGCGCTATCCCGCCTCGATCACCAAGGTCATGACCCTC
TACCTGACCTTCGAGGCGCTCAGCGAAGGCCGCCTGAAGCTCACCGATCAGATCGTGATGTCGCCTCGTGCGGCGGCGCA
GGCGCCGACCAAGATCGGCCTGCGTCCGGGCGAGAGCATGACCGTCGACGAGGCGATCAAGGCGATGGCGGTCAAGTCGG
CCAATGACGTCGCCACCGCCGTGGCCGAGCGACTGGCCGGCAGCGAGTCGCGCTTCGCCGCCCTGATGACCCTGCGCGGC
CAGGAGCTGGGCATGCGCAACACCCGCTTCGTCAACGCCTCGGGCCTGCCCGACAGCCGGCAGATCTCGACCGCCCGCGA
CCTGGCCATCCTGTCACGCGCCACGATGCGCGACTTCCCGCAGTACTACAGCTACTTCTCGGTGAAGGGCTTCTATTTCC
GGGGCAACTACGTCAAGGGCCACAACCGCCTGCTCGACAGCATGGAAGGGTTCGACGGCCTGAAGACCGGCTACACCAAC
GCCTCGGGCTTCAACCTCGCCGGCTCGGCCGTGCGCGACGGTCGTCGCCTGATCGCCGTGGTCCTGGGCGGCCCCTCGAC
CGCCTGGCGCGACAACAACATGGAAGACCTGCTGCTGACCGGCTTTGACGTGATCAAGCGCCGGTCGCGTGGTGAGCGCA
CCACCATCGCGGCCAACATCTATGAGGACGAGCCCTCGGGCCCGATCGAGCGTCCGTCGGCCGAGCAAGGCGACGGCGAC
CAGGCCGGCCTCAGCATCGTCCTGACCGAAAACCCCCGTTCCGCGCCCGTGAAGGTCTCCCCCACCCTGCGCGGCGCCCA
GGCGGCGACGCCGGTCAAGGCGGCGAAGAAGCCTAAGGGCGAATGGGGCGTGCAGGTCGGCGCCTTCCGCTCCAAGTCGC
TGGCCAACGAACAGTTGAAGCTGGTGCGCGGCCGGATCACCAAGCTGGTCAGCGACGCCGAGGGCGCCGTTGAGGGTGCT
GGCGGCATGTTCCGGGCGCAGTTCCAAGGCATGACCAACGAAGCCGCCCGCGAGGCCTGCTCGGCCCTGAAGGCCAAGCG
CATGCCCTGCATTGTGCTGAAGCCGTAG

Upstream 100 bases:

>100_bases
ACCAGTTGTTTACTTTGGCGAAACACCGCATCCATCATGTTAGGCTCTACGCGTGTGGCGGTGCGGAACCGCTTCCAGTT
CAAATGACGGACGTCGCTTC

Downstream 100 bases:

>100_bases
GCGAAACCCACCGCCGATTTCCCCGGCGAACGCCGAGGCCAGACGAACCAAGAGCGCCGGCGGCTTTGCTCCGGCGCTCT
TTCGTATTCACGGCCAAGTT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MFARLTSARSLLAAAGLALTCLAGTLAPSAASAAVPYLQLNASEPKYAAIVIDANSGEVLYDKRGDSPRYPASITKVMTL
YLTFEALSEGRLKLTDQIVMSPRAAAQAPTKIGLRPGESMTVDEAIKAMAVKSANDVATAVAERLAGSESRFAALMTLRG
QELGMRNTRFVNASGLPDSRQISTARDLAILSRATMRDFPQYYSYFSVKGFYFRGNYVKGHNRLLDSMEGFDGLKTGYTN
ASGFNLAGSAVRDGRRLIAVVLGGPSTAWRDNNMEDLLLTGFDVIKRRSRGERTTIAANIYEDEPSGPIERPSAEQGDGD
QAGLSIVLTENPRSAPVKVSPTLRGAQAATPVKAAKKPKGEWGVQVGAFRSKSLANEQLKLVRGRITKLVSDAEGAVEGA
GGMFRAQFQGMTNEAAREACSALKAKRMPCIVLKP

Sequences:

>Translated_435_residues
MFARLTSARSLLAAAGLALTCLAGTLAPSAASAAVPYLQLNASEPKYAAIVIDANSGEVLYDKRGDSPRYPASITKVMTL
YLTFEALSEGRLKLTDQIVMSPRAAAQAPTKIGLRPGESMTVDEAIKAMAVKSANDVATAVAERLAGSESRFAALMTLRG
QELGMRNTRFVNASGLPDSRQISTARDLAILSRATMRDFPQYYSYFSVKGFYFRGNYVKGHNRLLDSMEGFDGLKTGYTN
ASGFNLAGSAVRDGRRLIAVVLGGPSTAWRDNNMEDLLLTGFDVIKRRSRGERTTIAANIYEDEPSGPIERPSAEQGDGD
QAGLSIVLTENPRSAPVKVSPTLRGAQAATPVKAAKKPKGEWGVQVGAFRSKSLANEQLKLVRGRITKLVSDAEGAVEGA
GGMFRAQFQGMTNEAAREACSALKAKRMPCIVLKP
>Mature_435_residues
MFARLTSARSLLAAAGLALTCLAGTLAPSAASAAVPYLQLNASEPKYAAIVIDANSGEVLYDKRGDSPRYPASITKVMTL
YLTFEALSEGRLKLTDQIVMSPRAAAQAPTKIGLRPGESMTVDEAIKAMAVKSANDVATAVAERLAGSESRFAALMTLRG
QELGMRNTRFVNASGLPDSRQISTARDLAILSRATMRDFPQYYSYFSVKGFYFRGNYVKGHNRLLDSMEGFDGLKTGYTN
ASGFNLAGSAVRDGRRLIAVVLGGPSTAWRDNNMEDLLLTGFDVIKRRSRGERTTIAANIYEDEPSGPIERPSAEQGDGD
QAGLSIVLTENPRSAPVKVSPTLRGAQAATPVKAAKKPKGEWGVQVGAFRSKSLANEQLKLVRGRITKLVSDAEGAVEGA
GGMFRAQFQGMTNEAAREACSALKAKRMPCIVLKP

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=361, Percent_Identity=32.9639889196676, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1787062, Length=242, Percent_Identity=38.0165289256198, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87082022, Length=362, Percent_Identity=29.5580110497238, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082054, Length=245, Percent_Identity=27.3469387755102, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46512; Mature: 46512

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFARLTSARSLLAAAGLALTCLAGTLAPSAASAAVPYLQLNASEPKYAAIVIDANSGEVL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCEEEEEEECCCCCEE
YDKRGDSPRYPASITKVMTLYLTFEALSEGRLKLTDQIVMSPRAAAQAPTKIGLRPGESM
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHCCCCEECCCCCCCC
TVDEAIKAMAVKSANDVATAVAERLAGSESRFAALMTLRGQELGMRNTRFVNASGLPDSR
CHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCEEEECCCCCCCC
QISTARDLAILSRATMRDFPQYYSYFSVKGFYFRGNYVKGHNRLLDSMEGFDGLKTGYTN
CHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCEECCHHHHHHHHCCCCCHHCCCCC
ASGFNLAGSAVRDGRRLIAVVLGGPSTAWRDNNMEDLLLTGFDVIKRRSRGERTTIAANI
CCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEE
YEDEPSGPIERPSAEQGDGDQAGLSIVLTENPRSAPVKVSPTLRGAQAATPVKAAKKPKG
ECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCHHHHCCCCC
EWGVQVGAFRSKSLANEQLKLVRGRITKLVSDAEGAVEGAGGMFRAQFQGMTNEAAREAC
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHCCCCHHHHHHHH
SALKAKRMPCIVLKP
HHHHHCCCCEEEECC
>Mature Secondary Structure
MFARLTSARSLLAAAGLALTCLAGTLAPSAASAAVPYLQLNASEPKYAAIVIDANSGEVL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCEEEEEEECCCCCEE
YDKRGDSPRYPASITKVMTLYLTFEALSEGRLKLTDQIVMSPRAAAQAPTKIGLRPGESM
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHCCCCEECCCCCCCC
TVDEAIKAMAVKSANDVATAVAERLAGSESRFAALMTLRGQELGMRNTRFVNASGLPDSR
CHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCEEEECCCCCCCC
QISTARDLAILSRATMRDFPQYYSYFSVKGFYFRGNYVKGHNRLLDSMEGFDGLKTGYTN
CHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCEECCHHHHHHHHCCCCCHHCCCCC
ASGFNLAGSAVRDGRRLIAVVLGGPSTAWRDNNMEDLLLTGFDVIKRRSRGERTTIAANI
CCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEE
YEDEPSGPIERPSAEQGDGDQAGLSIVLTENPRSAPVKVSPTLRGAQAATPVKAAKKPKG
ECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCHHHHCCCCC
EWGVQVGAFRSKSLANEQLKLVRGRITKLVSDAEGAVEGAGGMFRAQFQGMTNEAAREAC
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHCCCCHHHHHHHH
SALKAKRMPCIVLKP
HHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]