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The map label for this gene is thyA

Identifier: 16126363

GI number: 16126363

Start: 2330013

End: 2330852

Strand: Direct

Name: thyA

Synonym: CC_2124

Alternate gene names: 16126363

Gene position: 2330013-2330852 (Clockwise)

Preceding gene: 16126362

Following gene: 16126365

Centisome position: 58.0

GC content: 64.88

Gene sequence:

>840_bases
ATGAGCACCGCCGTCCTCGACGCCCCCAAAGCCGCGACCGACCATCCCGAGCGGCAGTACCTGACCCTGCTGGCCGACAT
CCTGGAGAATGGCGTCCAGCGCGGCGACCGCACGGGCACGGGGACACTGGGCGTATTCGGCCGCCAGATCCGCTTTGACC
TGGCCAAGGGCTTCCCGGTCCTGACCACCAAGAAGCTGCACCTGCGCTCGATCATCATCGAGCTGCTGTGGTTCCTGAAG
GGCGACACCAACATCGCCTATCTCAAGGACAACGGCGTGCGCATCTGGGACGAGTGGGCCGACGAGAACGGCGACCTGGG
TCCCGTCTATGGCAAGCAGTGGCGCTCCTGGGCCACGCCTGACGGCCGGGTGATCGACCAGATCTCGGCCCTGGTCGAGA
ACCTGAAGGCCAACCCCAACAGCCGCCGCCACATCGTCACGGCCTGGAACCCGGCCGATGTCGACGACATGGCCCTGCCG
CCTTGCCACTGCCTGTTCCAGTTCTTCGTGGCGGATGGAAAACTGAGCTGCCAGCTCTACCAGCGCAGCGCCGACGTGTT
CTTAGGGGTGCCGTTCAACATCGCCAGCTACGCCCTGCTCACCCTGATGGTCGCCAAGGTCGTGGGCCTGGAGCCCGGCG
AGTTCGTCCACACCTTCGGCGACGCCCACCTCTATCTGAACCACCTGGATCAGGCCCGCGAGCAACTGACCCGCGAGCCC
TTCGACTTTCCCACGATGAAGATCGCCGACAAGCGCGACCTCTTCGGGTTCGAGTACGAGGACTTCACGCTGGAGGGCTA
TCAGGCCCACCCGCACATCAAGGCCGAGGTTTCGGTCTAG

Upstream 100 bases:

>100_bases
TCGAGCGAGGGCCAGAACGCGAGGACAAATGGCGGAGCCCTGGGGACATCACGGGGCGCCGCAGTGTTCAACACTGCGAT
TCGCGGCCAAAGTAACCGCC

Downstream 100 bases:

>100_bases
GGTTTCAAAACGATCTTGCCGGCCAGCTGCGCCGACTGCAGCAGGGCCTGAGCCTCCCCGGCCTCGCTGAGCGGCAGGAC
CTTGGCGATCTTCGGCTGGA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSTAVLDAPKAATDHPERQYLTLLADILENGVQRGDRTGTGTLGVFGRQIRFDLAKGFPVLTTKKLHLRSIIIELLWFLK
GDTNIAYLKDNGVRIWDEWADENGDLGPVYGKQWRSWATPDGRVIDQISALVENLKANPNSRRHIVTAWNPADVDDMALP
PCHCLFQFFVADGKLSCQLYQRSADVFLGVPFNIASYALLTLMVAKVVGLEPGEFVHTFGDAHLYLNHLDQAREQLTREP
FDFPTMKIADKRDLFGFEYEDFTLEGYQAHPHIKAEVSV

Sequences:

>Translated_279_residues
MSTAVLDAPKAATDHPERQYLTLLADILENGVQRGDRTGTGTLGVFGRQIRFDLAKGFPVLTTKKLHLRSIIIELLWFLK
GDTNIAYLKDNGVRIWDEWADENGDLGPVYGKQWRSWATPDGRVIDQISALVENLKANPNSRRHIVTAWNPADVDDMALP
PCHCLFQFFVADGKLSCQLYQRSADVFLGVPFNIASYALLTLMVAKVVGLEPGEFVHTFGDAHLYLNHLDQAREQLTREP
FDFPTMKIADKRDLFGFEYEDFTLEGYQAHPHIKAEVSV
>Mature_278_residues
STAVLDAPKAATDHPERQYLTLLADILENGVQRGDRTGTGTLGVFGRQIRFDLAKGFPVLTTKKLHLRSIIIELLWFLKG
DTNIAYLKDNGVRIWDEWADENGDLGPVYGKQWRSWATPDGRVIDQISALVENLKANPNSRRHIVTAWNPADVDDMALPP
CHCLFQFFVADGKLSCQLYQRSADVFLGVPFNIASYALLTLMVAKVVGLEPGEFVHTFGDAHLYLNHLDQAREQLTREPF
DFPTMKIADKRDLFGFEYEDFTLEGYQAHPHIKAEVSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=286, Percent_Identity=50.3496503496504, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1789191, Length=263, Percent_Identity=63.8783269961977, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71993377, Length=288, Percent_Identity=44.4444444444444, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324648, Length=293, Percent_Identity=43.3447098976109, Blast_Score=213, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17137556, Length=293, Percent_Identity=49.8293515358362, Blast_Score=285, Evalue=3e-77,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_CAUCR (Q9A6H0)

Other databases:

- EMBL:   AE005673
- PIR:   C87512
- RefSeq:   NP_420927.1
- ProteinModelPortal:   Q9A6H0
- SMR:   Q9A6H0
- GeneID:   943048
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_2124
- NMPDR:   fig|190650.1.peg.2107
- TIGR:   CC_2124
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 31462; Mature: 31331

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAVLDAPKAATDHPERQYLTLLADILENGVQRGDRTGTGTLGVFGRQIRFDLAKGFPV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCE
LTTKKLHLRSIIIELLWFLKGDTNIAYLKDNGVRIWDEWADENGDLGPVYGKQWRSWATP
EECCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCHHHCCCCCC
DGRVIDQISALVENLKANPNSRRHIVTAWNPADVDDMALPPCHCLFQFFVADGKLSCQLY
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEE
QRSADVFLGVPFNIASYALLTLMVAKVVGLEPGEFVHTFGDAHLYLNHLDQAREQLTREP
ECCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCC
FDFPTMKIADKRDLFGFEYEDFTLEGYQAHPHIKAEVSV
CCCCCEEECCCCCCCCCCCCCEEEECEECCCCEEEEECC
>Mature Secondary Structure 
STAVLDAPKAATDHPERQYLTLLADILENGVQRGDRTGTGTLGVFGRQIRFDLAKGFPV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCE
LTTKKLHLRSIIIELLWFLKGDTNIAYLKDNGVRIWDEWADENGDLGPVYGKQWRSWATP
EECCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCHHHCCCCCC
DGRVIDQISALVENLKANPNSRRHIVTAWNPADVDDMALPPCHCLFQFFVADGKLSCQLY
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEE
QRSADVFLGVPFNIASYALLTLMVAKVVGLEPGEFVHTFGDAHLYLNHLDQAREQLTREP
ECCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCC
FDFPTMKIADKRDLFGFEYEDFTLEGYQAHPHIKAEVSV
CCCCCEEECCCCCCCCCCCCCEEEECEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647