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The map label for this gene is xthA [H]

Identifier: 16126254

GI number: 16126254

Start: 2212365

End: 2213153

Strand: Direct

Name: xthA [H]

Synonym: CC_2011

Alternate gene names: 16126254

Gene position: 2212365-2213153 (Clockwise)

Preceding gene: 16126252

Following gene: 16126255

Centisome position: 55.08

GC content: 63.88

Gene sequence:

>789_bases
ATGCGTATCGCCACCTGGAACGTCAATTCGATCAATGCTCGCCTGGAAGGGGTATTGGCGTGGTTCGAATCGGAAGCTCC
CGACGTGGCCGTGCTGCAGGAGATCAAGTGCGTCGACGAAAAGTTCCCGACCGAAGCCTTTGAACGGCTTGGCTATAACG
TCGCGGTTCACGGCCAGAAGACCTATAATGGCGTGGCCCTGCTCTCGAAGCATCCCATCGAGGATGTCCGCAAGGGCCTG
CCCTTCCTGTCCGAGGACGAGGTCGACGAGCAGGCGCGCTATATCGAGGCGGTGATCGCCGCGCCGACGCCGTTCCGCGT
CGTGGGCATCTATTTGCCGAACGGCAATCCGATCGGGACTGAGAAGTTCGCCTACAAGCTTTCCTGGATGCGCCGCTTGC
ACGCCCACGCCCAAGGCCTCTTGGCGTTCGAGGAACCGCTGGTGATCGCCGGCGACTACAACGTCATTCCGGAGGTCGAG
GACGTCGCCAACCCGCAGGCCTGGCTGGGCGACGCCCTGTTTCAGCCGGAGAGCCGCGCAGCCTTCCGCGCCTTGAAGAA
CCTGGGCTTCGCCGACGCCTATATGCAGGCCGACGGCGCGCCGGGCGGCTACACCTTCTGGGATTATCAGGCCGGCGCCT
GGCAGCGGAACCTCGGCATCCGCATCGACCACCTGCTGCTGTCGCCGCAGGCGGCCGACCGTTTGACGGGCCTCGTCATC
CACCGCGACGAACGCGACAAGGAAAAGCCCTCGGACCACGTGCCGGTGGTCGGTCGTTTCCGTATCTAG

Upstream 100 bases:

>100_bases
AGCGTCATCAACGCCAGCTGCGTGCGGTCGACAGACTTTTTCCCCGACTTGAGCGCGGCCTTCATGCGCCCTAGCCTCCC
GCCGCACCTTGGAGCCGTCA

Downstream 100 bases:

>100_bases
GGGCTGCCCCTGTCCCGGAATCGGGACTCCCTCTAGCCTGTGCCCACTATGAGCGAACTCGCGCGTCTTTTGCTGTTCGT
GGCCATCGCCGGCTCGGCGG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRIATWNVNSINARLEGVLAWFESEAPDVAVLQEIKCVDEKFPTEAFERLGYNVAVHGQKTYNGVALLSKHPIEDVRKGL
PFLSEDEVDEQARYIEAVIAAPTPFRVVGIYLPNGNPIGTEKFAYKLSWMRRLHAHAQGLLAFEEPLVIAGDYNVIPEVE
DVANPQAWLGDALFQPESRAAFRALKNLGFADAYMQADGAPGGYTFWDYQAGAWQRNLGIRIDHLLLSPQAADRLTGLVI
HRDERDKEKPSDHVPVVGRFRI

Sequences:

>Translated_262_residues
MRIATWNVNSINARLEGVLAWFESEAPDVAVLQEIKCVDEKFPTEAFERLGYNVAVHGQKTYNGVALLSKHPIEDVRKGL
PFLSEDEVDEQARYIEAVIAAPTPFRVVGIYLPNGNPIGTEKFAYKLSWMRRLHAHAQGLLAFEEPLVIAGDYNVIPEVE
DVANPQAWLGDALFQPESRAAFRALKNLGFADAYMQADGAPGGYTFWDYQAGAWQRNLGIRIDHLLLSPQAADRLTGLVI
HRDERDKEKPSDHVPVVGRFRI
>Mature_262_residues
MRIATWNVNSINARLEGVLAWFESEAPDVAVLQEIKCVDEKFPTEAFERLGYNVAVHGQKTYNGVALLSKHPIEDVRKGL
PFLSEDEVDEQARYIEAVIAAPTPFRVVGIYLPNGNPIGTEKFAYKLSWMRRLHAHAQGLLAFEEPLVIAGDYNVIPEVE
DVANPQAWLGDALFQPESRAAFRALKNLGFADAYMQADGAPGGYTFWDYQAGAWQRNLGIRIDHLLLSPQAADRLTGLVI
HRDERDKEKPSDHVPVVGRFRI

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1788046, Length=273, Percent_Identity=35.1648351648352, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71989536, Length=269, Percent_Identity=29.368029739777, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221330655, Length=270, Percent_Identity=32.2222222222222, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17136678, Length=270, Percent_Identity=32.2222222222222, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29352; Mature: 29352

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNVNSINARLEGVLAWFESEAPDVAVLQEIKCVDEKFPTEAFERLGYNVAVHGQK
CEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCEEEEECCC
TYNGVALLSKHPIEDVRKGLPFLSEDEVDEQARYIEAVIAAPTPFRVVGIYLPNGNPIGT
CCCCEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCC
EKFAYKLSWMRRLHAHAQGLLAFEEPLVIAGDYNVIPEVEDVANPQAWLGDALFQPESRA
HHHHHHHHHHHHHHHHHHCCEEECCCEEEECCCCCCCCHHHHCCCHHHHHHHHCCCCHHH
AFRALKNLGFADAYMQADGAPGGYTFWDYQAGAWQRNLGIRIDHLLLSPQAADRLTGLVI
HHHHHHHCCCHHHHHCCCCCCCCCEEEECCCCCEECCCCCEEEEEEECCCHHHHHEEEEE
HRDERDKEKPSDHVPVVGRFRI
ECCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MRIATWNVNSINARLEGVLAWFESEAPDVAVLQEIKCVDEKFPTEAFERLGYNVAVHGQK
CEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCEEEEECCC
TYNGVALLSKHPIEDVRKGLPFLSEDEVDEQARYIEAVIAAPTPFRVVGIYLPNGNPIGT
CCCCEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCC
EKFAYKLSWMRRLHAHAQGLLAFEEPLVIAGDYNVIPEVEDVANPQAWLGDALFQPESRA
HHHHHHHHHHHHHHHHHHCCEEECCCEEEECCCCCCCCHHHHCCCHHHHHHHHCCCCHHH
AFRALKNLGFADAYMQADGAPGGYTFWDYQAGAWQRNLGIRIDHLLLSPQAADRLTGLVI
HHHHHHHCCCHHHHHCCCCCCCCCEEEECCCCCEECCCCCEEEEEEECCCHHHHHEEEEE
HRDERDKEKPSDHVPVVGRFRI
ECCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]