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The map label for this gene is parE

Identifier: 16126217

GI number: 16126217

Start: 2174469

End: 2176556

Strand: Direct

Name: parE

Synonym: CC_1974

Alternate gene names: 16126217

Gene position: 2174469-2176556 (Clockwise)

Preceding gene: 16126216

Following gene: 16126221

Centisome position: 54.13

GC content: 67.91

Gene sequence:

>2088_bases
ATGTCCTCCTCCGATAAGATCCCCTCCCTGTTTGGTGACGACGACGCCCTGGCGCCCGTCCCCGCCGCGCCGTTCAAGGC
CAGCGTGGAGCCGCGCGTCGAGCCGACGCCGCGCCCCATCCCGCCTCCGCCGCCGTCGAAGACCGCCTCCGCGCCCGGCG
AGTACTCGGCCGCCGACATCGAGGTGCTGGAAGGCCTGGAGCCGGTGCGCAAGCGGCCGGGCATGTACATCGGCGGCACC
GACGAGCGGGCCCTGCACCACCTGTTCGCCGAGGTGCTCGACAACTCGATGGACGAGGCCGTGGCCGGCTTCGCCAAGAC
CATCGAGGTCAAGCTGGACGCCGACGGTTTCCTGTCGGTCAAGGACGACGGTCGCGGCATGCCCGTCGACCCGCACCCCA
AGTATCCCGGCAAGTCGGCGCTGGAGGTCATCATGACCGTCCTGCACGCCGGCGGTAAGTTCACGGGCAAGGCCTACGAG
ACCTCCGGCGGCCTGCACGGCGTCGGCGCTTCCGTCGTCAACGCCCTGTCCGAGCGCGTCGAGGTCACCGTCTGGCGCGA
CGGCTTCGAGCACCTCCAGGTGTTCTCGCGCGGCAAGCCGCTGGGGCCGATCCAGCAGGTCGCGCCGTCGAAGAAGAAGG
GCACCATGGTGCGCTTCAAGCCCGACGATGAGATCTTCGGCGAGGGGACCAATTTCAAACCCGCGCGCCTTTACCGCATG
GCCCGTTCCAAGGCCTATCTGTTCCGCGGCGTGCAGATCAAATGGTCCTGTGATCCCTCGCGGATCCATGACCAGACCCC
GCCCGAGGCCACGTTCCACTTCCCCAACGGCCTGGCCGACTTCCTGGCCGAGCGGACCAAAGGCCTGACAACGATCACGC
CCGAAAGCTTCGCAGGCCGCATCGAACGCCAGGGCGAGGCCGGCGCGGTCGAGTGGGCCGTGACCTGGACGCCCCAAGGC
TTTGGCGAGCACGACGGCTTCATGCAGTCGTACTGCAACACCGTCCCGACGCCCGAAGGCGGCACCCACGAGAGCGGCTT
CCGCGCCGCCCTGACCCGCGGTCTCAAGGCCTATGCCGAGCTCAAGGGCGAGAAGCGCGGGACGATCATCACCGCCGACG
ACGTCGTCGCCCAGGCCGGCGCCCTGATCTCGGTGTTCATCAAGAACCCCGAATTCCAGGGCCAGACCAAGGAAAAGCTC
TCGACCTCCGAGGCCCAGCGCTTCGTCGAGGCCTCGCTGCGCGACCCGTTCGACCTGTGGCTGTCGTCCAGCCCCAAGAA
CGCCCAGGCCCTCTTGGAATTCGTCATCGAGCGGGCCGAAGAACGCCTCAAGCGCCGCAAGGACAAGGAAGTCTCCCGCG
CCTCGGCGACCCGCAAGCTGCGCCTGCCCGGCAAGCTGGCCGACTGCGCCGGGAGCGCGGTCGACGGCGCCGAGCTGTTC
ATCGTCGAAGGCGACAGCGCCGGCGGCTCGGCCAAGCAGGCCCGCGACCGCAAGTACCAGGCCATCCTGCCCCTGCGCGG
CAAGATCCTCAACGTGGCCTCGGCCAGCGGCGAGAAGTTCACCGCCAACAAGGAGCTGTCGGACCTGATGCTGGCCTTGG
GCGCCCAGGCCGGCGCCAAGTACCGCGAGGAAGATCTGCGCTACGAGCGGATCATCATCATGACCGACGCCGACGTCGAC
GGCGCCCACATCGCCTCGCTGCTGATCACCTTCTTCTACCGGACCATGCCCGAGCTGATCCGCGGCGGTCACCTGTTCCT
GGCCCTGCCGCCGCTGTACCGCCTGGCCCACGGCGGCAAGAGCGAGTACGCCCGCGACGACGCCCACAAGGAAGAGCTGC
TGGCCACGGTGTTCAAGGGCAAGAAGCCCGAGATCGGCCGCTTCAAGGGCCTGGGCGAGATGATGGCCTCCCAGCTGAAG
GAGACCACCATGGACCCCAAGAAGCGCACCCTGGCCCGCGTCACCCTGCCCCGCCACGAGGAAAGCGTCGAAGACCTGGT
CGAGACCCTGATGGGCCGCAAACCCGAACTGCGCTTCCGCTTCATCCAGGAAAACGCCGAGTTCGCGTCGGCGGATCTGG
ATTTGTAG

Upstream 100 bases:

>100_bases
GACACCTCTCCCACGGGGAGAGGATTTCATTTTCTCTTTTCAGGGGAAGGCTGGCCCGCCATACGAGGCCCCCGCGTTCG
AATCGCCTAGACTTCCCCTG

Downstream 100 bases:

>100_bases
GCGTCGGACTATCTCTCCTCCCCCTTTGGGGGAGGGGGACCGCCGAACGGCGGTGGAGGGGCCAGCCGAGCCGGCCGCGA
TCACCGTCAGGCAACCGGTC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MSSSDKIPSLFGDDDALAPVPAAPFKASVEPRVEPTPRPIPPPPPSKTASAPGEYSAADIEVLEGLEPVRKRPGMYIGGT
DERALHHLFAEVLDNSMDEAVAGFAKTIEVKLDADGFLSVKDDGRGMPVDPHPKYPGKSALEVIMTVLHAGGKFTGKAYE
TSGGLHGVGASVVNALSERVEVTVWRDGFEHLQVFSRGKPLGPIQQVAPSKKKGTMVRFKPDDEIFGEGTNFKPARLYRM
ARSKAYLFRGVQIKWSCDPSRIHDQTPPEATFHFPNGLADFLAERTKGLTTITPESFAGRIERQGEAGAVEWAVTWTPQG
FGEHDGFMQSYCNTVPTPEGGTHESGFRAALTRGLKAYAELKGEKRGTIITADDVVAQAGALISVFIKNPEFQGQTKEKL
STSEAQRFVEASLRDPFDLWLSSSPKNAQALLEFVIERAEERLKRRKDKEVSRASATRKLRLPGKLADCAGSAVDGAELF
IVEGDSAGGSAKQARDRKYQAILPLRGKILNVASASGEKFTANKELSDLMLALGAQAGAKYREEDLRYERIIIMTDADVD
GAHIASLLITFFYRTMPELIRGGHLFLALPPLYRLAHGGKSEYARDDAHKEELLATVFKGKKPEIGRFKGLGEMMASQLK
ETTMDPKKRTLARVTLPRHEESVEDLVETLMGRKPELRFRFIQENAEFASADLDL

Sequences:

>Translated_695_residues
MSSSDKIPSLFGDDDALAPVPAAPFKASVEPRVEPTPRPIPPPPPSKTASAPGEYSAADIEVLEGLEPVRKRPGMYIGGT
DERALHHLFAEVLDNSMDEAVAGFAKTIEVKLDADGFLSVKDDGRGMPVDPHPKYPGKSALEVIMTVLHAGGKFTGKAYE
TSGGLHGVGASVVNALSERVEVTVWRDGFEHLQVFSRGKPLGPIQQVAPSKKKGTMVRFKPDDEIFGEGTNFKPARLYRM
ARSKAYLFRGVQIKWSCDPSRIHDQTPPEATFHFPNGLADFLAERTKGLTTITPESFAGRIERQGEAGAVEWAVTWTPQG
FGEHDGFMQSYCNTVPTPEGGTHESGFRAALTRGLKAYAELKGEKRGTIITADDVVAQAGALISVFIKNPEFQGQTKEKL
STSEAQRFVEASLRDPFDLWLSSSPKNAQALLEFVIERAEERLKRRKDKEVSRASATRKLRLPGKLADCAGSAVDGAELF
IVEGDSAGGSAKQARDRKYQAILPLRGKILNVASASGEKFTANKELSDLMLALGAQAGAKYREEDLRYERIIIMTDADVD
GAHIASLLITFFYRTMPELIRGGHLFLALPPLYRLAHGGKSEYARDDAHKEELLATVFKGKKPEIGRFKGLGEMMASQLK
ETTMDPKKRTLARVTLPRHEESVEDLVETLMGRKPELRFRFIQENAEFASADLDL
>Mature_694_residues
SSSDKIPSLFGDDDALAPVPAAPFKASVEPRVEPTPRPIPPPPPSKTASAPGEYSAADIEVLEGLEPVRKRPGMYIGGTD
ERALHHLFAEVLDNSMDEAVAGFAKTIEVKLDADGFLSVKDDGRGMPVDPHPKYPGKSALEVIMTVLHAGGKFTGKAYET
SGGLHGVGASVVNALSERVEVTVWRDGFEHLQVFSRGKPLGPIQQVAPSKKKGTMVRFKPDDEIFGEGTNFKPARLYRMA
RSKAYLFRGVQIKWSCDPSRIHDQTPPEATFHFPNGLADFLAERTKGLTTITPESFAGRIERQGEAGAVEWAVTWTPQGF
GEHDGFMQSYCNTVPTPEGGTHESGFRAALTRGLKAYAELKGEKRGTIITADDVVAQAGALISVFIKNPEFQGQTKEKLS
TSEAQRFVEASLRDPFDLWLSSSPKNAQALLEFVIERAEERLKRRKDKEVSRASATRKLRLPGKLADCAGSAVDGAELFI
VEGDSAGGSAKQARDRKYQAILPLRGKILNVASASGEKFTANKELSDLMLALGAQAGAKYREEDLRYERIIIMTDADVDG
AHIASLLITFFYRTMPELIRGGHLFLALPPLYRLAHGGKSEYARDDAHKEELLATVFKGKKPEIGRFKGLGEMMASQLKE
TTMDPKKRTLARVTLPRHEESVEDLVETLMGRKPELRFRFIQENAEFASADLDL

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI19913406, Length=645, Percent_Identity=24.9612403100775, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI19913408, Length=648, Percent_Identity=25, Blast_Score=130, Evalue=5e-30,
Organism=Escherichia coli, GI1789408, Length=645, Percent_Identity=43.5658914728682, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI48994957, Length=562, Percent_Identity=45.017793594306, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17535065, Length=590, Percent_Identity=23.8983050847458, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI212645845, Length=573, Percent_Identity=24.6073298429319, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=201, Percent_Identity=27.8606965174129, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=648, Percent_Identity=26.2345679012346, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17136538, Length=606, Percent_Identity=25.2475247524752, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PARE_CAUCR (O54479)

Other databases:

- EMBL:   U86303
- EMBL:   AE005673
- EMBL:   U94697
- PIR:   A87494
- RefSeq:   NP_420781.1
- ProteinModelPortal:   O54479
- GeneID:   942751
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_1974
- NMPDR:   fig|190650.1.peg.1961
- TIGR:   CC_1974
- HOGENOM:   HBG304889
- OMA:   QARDKEF
- ProtClustDB:   PRK05559
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737
- InterPro:   IPR006171
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01055

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: 5.99.1.-

Molecular weight: Translated: 76164; Mature: 76033

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSDKIPSLFGDDDALAPVPAAPFKASVEPRVEPTPRPIPPPPPSKTASAPGEYSAADI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
EVLEGLEPVRKRPGMYIGGTDERALHHLFAEVLDNSMDEAVAGFAKTIEVKLDADGFLSV
HHHHCCHHHHHCCCEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHEEEEEEECCCCEEEE
KDDGRGMPVDPHPKYPGKSALEVIMTVLHAGGKFTGKAYETSGGLHGVGASVVNALSERV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHE
EVTVWRDGFEHLQVFSRGKPLGPIQQVAPSKKKGTMVRFKPDDEIFGEGTNFKPARLYRM
EEEEECCCHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEECCCHHHCCCCCCCCHHHHHHH
ARSKAYLFRGVQIKWSCDPSRIHDQTPPEATFHFPNGLADFLAERTKGLTTITPESFAGR
HHHHHHEEECEEEEECCCHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCEEECCHHHHHH
IERQGEAGAVEWAVTWTPQGFGEHDGFMQSYCNTVPTPEGGTHESGFRAALTRGLKAYAE
HHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LKGEKRGTIITADDVVAQAGALISVFIKNPEFQGQTKEKLSTSEAQRFVEASLRDPFDLW
HCCCCCCCEEEHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
LSSSPKNAQALLEFVIERAEERLKRRKDKEVSRASATRKLRLPGKLADCAGSAVDGAELF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCCCCCEEE
IVEGDSAGGSAKQARDRKYQAILPLRGKILNVASASGEKFTANKELSDLMLALGAQAGAK
EEECCCCCCCHHHHHHCCCEEECCCCCCEEEEECCCCCCEECCHHHHHHHHHHHHHCCCH
YREEDLRYERIIIMTDADVDGAHIASLLITFFYRTMPELIRGGHLFLALPPLYRLAHGGK
HHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCC
SEYARDDAHKEELLATVFKGKKPEIGRFKGLGEMMASQLKETTMDPKKRTLARVTLPRHE
CHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCH
ESVEDLVETLMGRKPELRFRFIQENAEFASADLDL
HHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCCCC
>Mature Secondary Structure 
SSSDKIPSLFGDDDALAPVPAAPFKASVEPRVEPTPRPIPPPPPSKTASAPGEYSAADI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
EVLEGLEPVRKRPGMYIGGTDERALHHLFAEVLDNSMDEAVAGFAKTIEVKLDADGFLSV
HHHHCCHHHHHCCCEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHEEEEEEECCCCEEEE
KDDGRGMPVDPHPKYPGKSALEVIMTVLHAGGKFTGKAYETSGGLHGVGASVVNALSERV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHE
EVTVWRDGFEHLQVFSRGKPLGPIQQVAPSKKKGTMVRFKPDDEIFGEGTNFKPARLYRM
EEEEECCCHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEECCCHHHCCCCCCCCHHHHHHH
ARSKAYLFRGVQIKWSCDPSRIHDQTPPEATFHFPNGLADFLAERTKGLTTITPESFAGR
HHHHHHEEECEEEEECCCHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCEEECCHHHHHH
IERQGEAGAVEWAVTWTPQGFGEHDGFMQSYCNTVPTPEGGTHESGFRAALTRGLKAYAE
HHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LKGEKRGTIITADDVVAQAGALISVFIKNPEFQGQTKEKLSTSEAQRFVEASLRDPFDLW
HCCCCCCCEEEHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
LSSSPKNAQALLEFVIERAEERLKRRKDKEVSRASATRKLRLPGKLADCAGSAVDGAELF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCCCCCEEE
IVEGDSAGGSAKQARDRKYQAILPLRGKILNVASASGEKFTANKELSDLMLALGAQAGAK
EEECCCCCCCHHHHHHCCCEEECCCCCCEEEEECCCCCCEECCHHHHHHHHHHHHHCCCH
YREEDLRYERIIIMTDADVDGAHIASLLITFFYRTMPELIRGGHLFLALPPLYRLAHGGK
HHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCC
SEYARDDAHKEELLATVFKGKKPEIGRFKGLGEMMASQLKETTMDPKKRTLARVTLPRHE
CHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCH
ESVEDLVETLMGRKPELRFRFIQENAEFASADLDL
HHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647; 9426128