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The map label for this gene is rsuA [H]

Identifier: 16126081

GI number: 16126081

Start: 2031961

End: 2032686

Strand: Direct

Name: rsuA [H]

Synonym: CC_1838

Alternate gene names: 16126081

Gene position: 2031961-2032686 (Clockwise)

Preceding gene: 16126080

Following gene: 16126082

Centisome position: 50.58

GC content: 67.77

Gene sequence:

>726_bases
ATGGCGCGGCTGGACCGGCTGCTGGCCAATCTGGGCTATGGCAGCCGCAAGGAGGTCCAGGCCCTGGTCGCCGGCGGCAA
GGTCGTGCTCGATGGGGCGATCCTGAAGGACGCCGGCGCCCGCATCGCGGTCGATGCGACCCTGCCGGAGCGGATGACCA
TTCGTGGCGCGCCCGTCGATCCGCCTGCGCCGCTGGTGCTGATCATGCACAAGCCGCTGGGCGTGGTGTGCTCGCACAAG
GAAGACGGCGAGAAGATCTATGATCTGCTGCCCCGCCGCTGGCGCTTACGGGACCCAGGGCTCTCAACGGTGGGCCGTCT
CGACAAGGACACCAGCGGCCTGATCCTGATCACCGACGATGGTGATTTCCTTCATCGGGTCATCTCGCCCAAGCGCCATG
TGCCCAAGACCTACCTGGCGACGCTGGACCGACCGCTGAACGGTTCCGAGGGCGAGGTCTTCGCCGCCGGAACGCTGATG
CTGGACAGCGAGGAAAAGCCGCTGCTCCCGGCCAGGCTGGACGTGGTGAACGCCCACGCAGCCCGTCTGACGATCACCGA
AGGGCGCTATCATCAGGTCCGCCGGATGTTCGCGGCGGTGGGCAATCACGTCGTGGCCCTGCATCGCGAGCGGATCGGCG
GCATCGCCCTGCCCGACGACCTTGCGGCCGGCCAGCATCGCATTCTCACTGCGGCGGCGGCCGAGCAGGTGTTCGCCGAT
GGCTGA

Upstream 100 bases:

>100_bases
CCTATGAGGCCATCCTGGGCGAAAGCTTCAGCAAGGTGCGACTGGTTGGCGAGGGCGGCGGTTACAAGGTCTTCGAGGCG
AAGAAATGAGCAAGGCGCTG

Downstream 100 bases:

>100_bases
GCCGATCGTTGTCGACGCGCGGGGCCATCACTGCCCGGTGCCGACCTTGAAGCTGCGCAAGGCGCATGAAGCGGCCCCCG
CCGGGGCGGAGCTCGTGCTG

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MARLDRLLANLGYGSRKEVQALVAGGKVVLDGAILKDAGARIAVDATLPERMTIRGAPVDPPAPLVLIMHKPLGVVCSHK
EDGEKIYDLLPRRWRLRDPGLSTVGRLDKDTSGLILITDDGDFLHRVISPKRHVPKTYLATLDRPLNGSEGEVFAAGTLM
LDSEEKPLLPARLDVVNAHAARLTITEGRYHQVRRMFAAVGNHVVALHRERIGGIALPDDLAAGQHRILTAAAAEQVFAD
G

Sequences:

>Translated_241_residues
MARLDRLLANLGYGSRKEVQALVAGGKVVLDGAILKDAGARIAVDATLPERMTIRGAPVDPPAPLVLIMHKPLGVVCSHK
EDGEKIYDLLPRRWRLRDPGLSTVGRLDKDTSGLILITDDGDFLHRVISPKRHVPKTYLATLDRPLNGSEGEVFAAGTLM
LDSEEKPLLPARLDVVNAHAARLTITEGRYHQVRRMFAAVGNHVVALHRERIGGIALPDDLAAGQHRILTAAAAEQVFAD
G
>Mature_240_residues
ARLDRLLANLGYGSRKEVQALVAGGKVVLDGAILKDAGARIAVDATLPERMTIRGAPVDPPAPLVLIMHKPLGVVCSHKE
DGEKIYDLLPRRWRLRDPGLSTVGRLDKDTSGLILITDDGDFLHRVISPKRHVPKTYLATLDRPLNGSEGEVFAAGTLML
DSEEKPLLPARLDVVNAHAARLTITEGRYHQVRRMFAAVGNHVVALHRERIGGIALPDDLAAGQHRILTAAAAEQVFADG

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=44.206008583691, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1787524, Length=234, Percent_Identity=32.0512820512821, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=29.3388429752066, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26093; Mature: 25961

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLDRLLANLGYGSRKEVQALVAGGKVVLDGAILKDAGARIAVDATLPERMTIRGAPVD
CCHHHHHHHHCCCCCHHHHHHHHCCCEEEECCEEECCCCCEEEEECCCCCCEEEECCCCC
PPAPLVLIMHKPLGVVCSHKEDGEKIYDLLPRRWRLRDPGLSTVGRLDKDTSGLILITDD
CCCCEEEEEECCCCEEECCCCCHHHHHHHCCCCEECCCCCCHHHHCCCCCCCCEEEEECC
GDFLHRVISPKRHVPKTYLATLDRPLNGSEGEVFAAGTLMLDSEEKPLLPARLDVVNAHA
HHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHEEECCE
ARLTITEGRYHQVRRMFAAVGNHVVALHRERIGGIALPDDLAAGQHRILTAAAAEQVFAD
EEEEEECCHHHHHHHHHHHHCCEEEEEEHHHCCCCCCCHHHCCCCHHEEHHHHHHHHHCC
G
C
>Mature Secondary Structure 
ARLDRLLANLGYGSRKEVQALVAGGKVVLDGAILKDAGARIAVDATLPERMTIRGAPVD
CHHHHHHHHCCCCCHHHHHHHHCCCEEEECCEEECCCCCEEEEECCCCCCEEEECCCCC
PPAPLVLIMHKPLGVVCSHKEDGEKIYDLLPRRWRLRDPGLSTVGRLDKDTSGLILITDD
CCCCEEEEEECCCCEEECCCCCHHHHHHHCCCCEECCCCCCHHHHCCCCCCCCEEEEECC
GDFLHRVISPKRHVPKTYLATLDRPLNGSEGEVFAAGTLMLDSEEKPLLPARLDVVNAHA
HHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHEEECCE
ARLTITEGRYHQVRRMFAAVGNHVVALHRERIGGIALPDDLAAGQHRILTAAAAEQVFAD
EEEEEECCHHHHHHHHHHHHCCEEEEEEHHHCCCCCCCHHHCCCCHHEEHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]