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The map label for this gene is lipA

Identifier: 16125979

GI number: 16125979

Start: 1908526

End: 1909503

Strand: Direct

Name: lipA

Synonym: CC_1735

Alternate gene names: 16125979

Gene position: 1908526-1909503 (Clockwise)

Preceding gene: 16125976

Following gene: 16125980

Centisome position: 47.51

GC content: 66.05

Gene sequence:

>978_bases
ATGGCCACGGTGATCGACACCCTGAAGGCTCGCGGCTCGGAAGACCGGGCGGCGCGCCATCCTGAAAAGCAGAACCGACC
GGACACGCCGGTGCTTCGCAAGCCTGAATGGCTGCGTGTGCGCGCGCCCGGCTCGGGGCAGTACAACGAGACCAAGGGCA
TCGTGCGCGAGCACAAGCTGCACACGGTCTGCGAAGAGGCGGCCTGCCCGAACATCGGCGAGTGCTGGAGCCAGAAGCAC
GCGACCATGATGATCATGGGCGAGATCTGCACCCGCGCCTGCGCCTTCTGCAATGTGACCACGGGCCTGCCGACGCAACT
GGATCCCGACGAGCCGCGCCGCGTGGCCGAGGCTGTCGCCAAGATGGGCCTCAAACACGTGGTCATCACCTCGGTGGACC
GCGACGACCTTCTGGACGGCGGCGCGCGCCATTTCGCCGAGGTGGTCACGTCCATCCGGGCCGCCGCCCCGGGCACGACG
ATCGAGATCCTGACCCCGGATTTCCTCCGCAAGGACGGCGCCGAGAACGTCGTCATCGACTCCAAGCCGGACGTCTTCAA
CCACAATCTCGAAACCGTGCCGCGGCTCTACCTGAAGATCCGTCCCGGCGCGCGCTACTACAACTCGCTGCGCCTGCTCG
ACCGCGTCAAGCAGCGTGACCCTTCGCAGTTCACCAAGTCGGGCCTGATGGTCGGTCTGGGTGAAACCAAGGAAGAGGTC
ATGCAGGTGATGGACGACATGCGCTCGGCGGGCGTCGATTTCATCACCATCGGCCAGTACCTGCAGCCGACGCGCAAGCA
CGCGGCGATCGACCGTTTCGTGACGCCTGAAGAGTTCAAGGCCTACGAGGCGATCGCGCGGGCTAAGGGCTTCCTGATGG
TGTCGTCCAGTCCGCTGACCCGCTCGTCGCACCACGCCGGCGAGGATTTCGCCAAGTTGCAGGCCGCGCGGCGCGCCCTC
GACGCTCGTACCGCCTAA

Upstream 100 bases:

>100_bases
GAGGAGACGGCGACGCATACGCGCCAGTCATTTCGCGCCTTGTGAGCTTACGCAAGATCAAGCGCTTGATCCGGAGGGCA
AGAAGGCCGATTTAGGCGCC

Downstream 100 bases:

>100_bases
GCCTTGCACCGTCACGTCGTTACGAAGGTGCTGCCCTATACGCCCGACCAGCTGTTCGAGCTGGTCGGAGACGTCGATGC
GTATCCGAAGTTCGTGCCCT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MATVIDTLKARGSEDRAARHPEKQNRPDTPVLRKPEWLRVRAPGSGQYNETKGIVREHKLHTVCEEAACPNIGECWSQKH
ATMMIMGEICTRACAFCNVTTGLPTQLDPDEPRRVAEAVAKMGLKHVVITSVDRDDLLDGGARHFAEVVTSIRAAAPGTT
IEILTPDFLRKDGAENVVIDSKPDVFNHNLETVPRLYLKIRPGARYYNSLRLLDRVKQRDPSQFTKSGLMVGLGETKEEV
MQVMDDMRSAGVDFITIGQYLQPTRKHAAIDRFVTPEEFKAYEAIARAKGFLMVSSSPLTRSSHHAGEDFAKLQAARRAL
DARTA

Sequences:

>Translated_325_residues
MATVIDTLKARGSEDRAARHPEKQNRPDTPVLRKPEWLRVRAPGSGQYNETKGIVREHKLHTVCEEAACPNIGECWSQKH
ATMMIMGEICTRACAFCNVTTGLPTQLDPDEPRRVAEAVAKMGLKHVVITSVDRDDLLDGGARHFAEVVTSIRAAAPGTT
IEILTPDFLRKDGAENVVIDSKPDVFNHNLETVPRLYLKIRPGARYYNSLRLLDRVKQRDPSQFTKSGLMVGLGETKEEV
MQVMDDMRSAGVDFITIGQYLQPTRKHAAIDRFVTPEEFKAYEAIARAKGFLMVSSSPLTRSSHHAGEDFAKLQAARRAL
DARTA
>Mature_324_residues
ATVIDTLKARGSEDRAARHPEKQNRPDTPVLRKPEWLRVRAPGSGQYNETKGIVREHKLHTVCEEAACPNIGECWSQKHA
TMMIMGEICTRACAFCNVTTGLPTQLDPDEPRRVAEAVAKMGLKHVVITSVDRDDLLDGGARHFAEVVTSIRAAAPGTTI
EILTPDFLRKDGAENVVIDSKPDVFNHNLETVPRLYLKIRPGARYYNSLRLLDRVKQRDPSQFTKSGLMVGLGETKEEVM
QVMDDMRSAGVDFITIGQYLQPTRKHAAIDRFVTPEEFKAYEAIARAKGFLMVSSSPLTRSSHHAGEDFAKLQAARRALD
ARTA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=46.5986394557823, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=46.9635627530364, Blast_Score=227, Evalue=9e-60,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=52.158273381295, Blast_Score=308, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=45.3488372093023, Blast_Score=235, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=307, Percent_Identity=43.9739413680782, Blast_Score=261, Evalue=8e-71,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=46.8531468531469, Blast_Score=262, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_CAUCN (B8GW82)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002517182.1
- ProteinModelPortal:   B8GW82
- GeneID:   7331276
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_01809
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36162; Mature: 36031

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVIDTLKARGSEDRAARHPEKQNRPDTPVLRKPEWLRVRAPGSGQYNETKGIVREHKL
CCHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
HTVCEEAACPNIGECWSQKHATMMIMGEICTRACAFCNVTTGLPTQLDPDEPRRVAEAVA
HHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
KMGLKHVVITSVDRDDLLDGGARHFAEVVTSIRAAAPGTTIEILTPDFLRKDGAENVVID
HCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCEEEC
SKPDVFNHNLETVPRLYLKIRPGARYYNSLRLLDRVKQRDPSQFTKSGLMVGLGETKEEV
CCCCHHCCCHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEECCCCHHHH
MQVMDDMRSAGVDFITIGQYLQPTRKHAAIDRFVTPEEFKAYEAIARAKGFLMVSSSPLT
HHHHHHHHHCCCCEEEHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCCC
RSSHHAGEDFAKLQAARRALDARTA
CCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATVIDTLKARGSEDRAARHPEKQNRPDTPVLRKPEWLRVRAPGSGQYNETKGIVREHKL
CHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
HTVCEEAACPNIGECWSQKHATMMIMGEICTRACAFCNVTTGLPTQLDPDEPRRVAEAVA
HHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
KMGLKHVVITSVDRDDLLDGGARHFAEVVTSIRAAAPGTTIEILTPDFLRKDGAENVVID
HCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCEEEC
SKPDVFNHNLETVPRLYLKIRPGARYYNSLRLLDRVKQRDPSQFTKSGLMVGLGETKEEV
CCCCHHCCCHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEECCCCHHHH
MQVMDDMRSAGVDFITIGQYLQPTRKHAAIDRFVTPEEFKAYEAIARAKGFLMVSSSPLT
HHHHHHHHHCCCCEEEHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCCC
RSSHHAGEDFAKLQAARRALDARTA
CCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA