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The map label for this gene is pdhC [H]

Identifier: 16125973

GI number: 16125973

Start: 1900945

End: 1902231

Strand: Direct

Name: pdhC [H]

Synonym: CC_1729

Alternate gene names: 16125973

Gene position: 1900945-1902231 (Clockwise)

Preceding gene: 16125972

Following gene: 16125974

Centisome position: 47.32

GC content: 68.84

Gene sequence:

>1287_bases
ATGTCGATCGACATCCTGATGCCCGCCCTGTCGCCCACCATGGAGGAGGGAACCCTCGCCAAGTGGCACGTCAAGGTCGG
CGACACCGTCAAGGCCGGCGACGTGATCGCCGAGATCGAGACCGACAAGGCGACGATGGAAGTCGAAGCCGTCGATGAAG
GCGTCGTGGAAGCCATCCTGGTTCCGGCCGGGACCGAGAACGTCAAGGTCAACGCCCTGATCGCCAAGCTGGCGGGCGAG
GGCGACAGCCCCGCGCCGGCCCCGAAGGTCGAAGCCCCGAAAGCCGCCGCCGCTGCGCCGGTTCCGGCCGCCGCCCCGGC
TCCGGCCGTGCCCGCGCCGGCCGCTCCGGTCGCCGCTGACGGCTCGCGCGTCCTGGCCTCGCCCCTGGCCCGCCGTCTGG
CTTCGGCCGCCGGTCTCGACTTGAAGGCCCTCAAGGGCACCGGCCCGCACGGCCGCGTCGTGAAGTCGGACGTCGAAGCC
GCCAAGTCGGGCGCGCCGGCCGCTAAGGCTGCTCCGGCCTCGGCGCCCGCCGCCGTCGCTCCGACCGCCGCCGCGCCGCG
TCAGATTCAGTCGCTGGAGCAGATGGGCATTCCCGCCGGCTCCTACGACCTCGTCCCGCTGGACGGCATGCGCAAGACGA
TCGCCCGTCGCATGACGGAGAGCTTCCGCGACGTGCCGCACTTCCCGCTCACGATCGATCTCGAGATCGACGCCCTGCTG
GCCGCCCGCGCCAAGATCAACAGCCTGCTGGAAAAGCAGGGCGTGAAGGTCTCGGTGAACGACATCGTCATCAAGGCCGC
CGCCGTGGCCCTGAAGCAGGTGCCGGAAGCCAACGCCAGCTACACGCCGGAAGGCATCGCCATGCACCACCACGCCGACA
TCGCCGTCGCCGTGGCCGTCGATGGCGGCCTGATCACCCCGATCATCCGCAAGGCCGAGACCAAGGGCCTGGCGCAGATC
TCCGCCGAGATGAAGGACCTGGCCCAGCGCGCCAAGGACAAGAAGCTGAAGCCCGAAGAGTTCCAGGGCGGCACCTTCTC
GATCAGCAACCTCGGCATGTTCGGCATCAAGTCGTTCGCCTCGATCATCAACGAGCCGCAAGGCGCGATCATGAGCGTCG
GCGCGGGCGAACAGCGTCCGGTCGTCAAGAACGGCGAGATCAAGGTCGCCACGGTCATGACCGTGACCTTGACCTGCGAT
CACCGCGTGGTCGACGGCTCGGTCGGCGCCAAGTTCCTGGCGGCCTTCAGGCCGCTGATTGAAGAGCCGCTGACCCTGAT
CGTCTGA

Upstream 100 bases:

>100_bases
CGGCGACAAAACGGACGAACAACGCCTTGCCCGTCGCAAGGCCAAATGGACGCCGACCACCTTGGTCGAAGCCGTCCCGA
ACGATTGAGGAAAAAGCTCA

Downstream 100 bases:

>100_bases
TCAGGGCGCGGGGAAGGTATGGCCATGTCGATCTACGACTATTCCGCCAAGACGCTGGACGGCCAGGACGTGAGCCTGGC
CGACTATCGCGGCCAAGTGC

Product: pyruvate dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSIDILMPALSPTMEEGTLAKWHVKVGDTVKAGDVIAEIETDKATMEVEAVDEGVVEAILVPAGTENVKVNALIAKLAGE
GDSPAPAPKVEAPKAAAAAPVPAAAPAPAVPAPAAPVAADGSRVLASPLARRLASAAGLDLKALKGTGPHGRVVKSDVEA
AKSGAPAAKAAPASAPAAVAPTAAAPRQIQSLEQMGIPAGSYDLVPLDGMRKTIARRMTESFRDVPHFPLTIDLEIDALL
AARAKINSLLEKQGVKVSVNDIVIKAAAVALKQVPEANASYTPEGIAMHHHADIAVAVAVDGGLITPIIRKAETKGLAQI
SAEMKDLAQRAKDKKLKPEEFQGGTFSISNLGMFGIKSFASIINEPQGAIMSVGAGEQRPVVKNGEIKVATVMTVTLTCD
HRVVDGSVGAKFLAAFRPLIEEPLTLIV

Sequences:

>Translated_428_residues
MSIDILMPALSPTMEEGTLAKWHVKVGDTVKAGDVIAEIETDKATMEVEAVDEGVVEAILVPAGTENVKVNALIAKLAGE
GDSPAPAPKVEAPKAAAAAPVPAAAPAPAVPAPAAPVAADGSRVLASPLARRLASAAGLDLKALKGTGPHGRVVKSDVEA
AKSGAPAAKAAPASAPAAVAPTAAAPRQIQSLEQMGIPAGSYDLVPLDGMRKTIARRMTESFRDVPHFPLTIDLEIDALL
AARAKINSLLEKQGVKVSVNDIVIKAAAVALKQVPEANASYTPEGIAMHHHADIAVAVAVDGGLITPIIRKAETKGLAQI
SAEMKDLAQRAKDKKLKPEEFQGGTFSISNLGMFGIKSFASIINEPQGAIMSVGAGEQRPVVKNGEIKVATVMTVTLTCD
HRVVDGSVGAKFLAAFRPLIEEPLTLIV
>Mature_427_residues
SIDILMPALSPTMEEGTLAKWHVKVGDTVKAGDVIAEIETDKATMEVEAVDEGVVEAILVPAGTENVKVNALIAKLAGEG
DSPAPAPKVEAPKAAAAAPVPAAAPAPAVPAPAAPVAADGSRVLASPLARRLASAAGLDLKALKGTGPHGRVVKSDVEAA
KSGAPAAKAAPASAPAAVAPTAAAPRQIQSLEQMGIPAGSYDLVPLDGMRKTIARRMTESFRDVPHFPLTIDLEIDALLA
ARAKINSLLEKQGVKVSVNDIVIKAAAVALKQVPEANASYTPEGIAMHHHADIAVAVAVDGGLITPIIRKAETKGLAQIS
AEMKDLAQRAKDKKLKPEEFQGGTFSISNLGMFGIKSFASIINEPQGAIMSVGAGEQRPVVKNGEIKVATVMTVTLTCDH
RVVDGSVGAKFLAAFRPLIEEPLTLIV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=444, Percent_Identity=41.8918918918919, Blast_Score=331, Evalue=7e-91,
Organism=Homo sapiens, GI203098753, Length=460, Percent_Identity=38.695652173913, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=38.695652173913, Blast_Score=278, Evalue=8e-75,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=45.3416149068323, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=25.4079254079254, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=32.8888888888889, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1786946, Length=430, Percent_Identity=32.3255813953488, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1786305, Length=412, Percent_Identity=32.2815533980583, Blast_Score=167, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=451, Percent_Identity=42.5720620842572, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=37.4603174603175, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17537937, Length=417, Percent_Identity=29.9760191846523, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=431, Percent_Identity=30.8584686774942, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=38.3073496659243, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6320352, Length=429, Percent_Identity=29.6037296037296, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321632, Length=170, Percent_Identity=35.8823529411765, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20129315, Length=452, Percent_Identity=41.3716814159292, Blast_Score=268, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24582497, Length=441, Percent_Identity=40.8163265306122, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=28.4382284382284, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=33.6283185840708, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44011; Mature: 43880

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDILMPALSPTMEEGTLAKWHVKVGDTVKAGDVIAEIETDKATMEVEAVDEGVVEAIL
CCCEEEECCCCCCCCCCCEEEEEEEECCEEECCCEEEEEECCCCEEEEHHHHCCCEEEEE
VPAGTENVKVNALIAKLAGEGDSPAPAPKVEAPKAAAAAPVPAAAPAPAVPAPAAPVAAD
ECCCCCCEEHHEEHEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GSRVLASPLARRLASAAGLDLKALKGTGPHGRVVKSDVEAAKSGAPAAKAAPASAPAAVA
CCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCC
PTAAAPRQIQSLEQMGIPAGSYDLVPLDGMRKTIARRMTESFRDVPHFPLTIDLEIDALL
CCCCCHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHH
AARAKINSLLEKQGVKVSVNDIVIKAAAVALKQVPEANASYTPEGIAMHHHADIAVAVAV
HHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEEE
DGGLITPIIRKAETKGLAQISAEMKDLAQRAKDKKLKPEEFQGGTFSISNLGMFGIKSFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEECCCCHHHHHHHH
SIINEPQGAIMSVGAGEQRPVVKNGEIKVATVMTVTLTCDHRVVDGSVGAKFLAAFRPLI
HHHCCCCCCEEECCCCCCCCCEECCCEEEEEEEEEEEEECCEEECCCHHHHHHHHHHHHH
EEPLTLIV
HCCHHHCC
>Mature Secondary Structure 
SIDILMPALSPTMEEGTLAKWHVKVGDTVKAGDVIAEIETDKATMEVEAVDEGVVEAIL
CCEEEECCCCCCCCCCCEEEEEEEECCEEECCCEEEEEECCCCEEEEHHHHCCCEEEEE
VPAGTENVKVNALIAKLAGEGDSPAPAPKVEAPKAAAAAPVPAAAPAPAVPAPAAPVAAD
ECCCCCCEEHHEEHEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GSRVLASPLARRLASAAGLDLKALKGTGPHGRVVKSDVEAAKSGAPAAKAAPASAPAAVA
CCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCC
PTAAAPRQIQSLEQMGIPAGSYDLVPLDGMRKTIARRMTESFRDVPHFPLTIDLEIDALL
CCCCCHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHH
AARAKINSLLEKQGVKVSVNDIVIKAAAVALKQVPEANASYTPEGIAMHHHADIAVAVAV
HHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEEE
DGGLITPIIRKAETKGLAQISAEMKDLAQRAKDKKLKPEEFQGGTFSISNLGMFGIKSFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEECCCCHHHHHHHH
SIINEPQGAIMSVGAGEQRPVVKNGEIKVATVMTVTLTCDHRVVDGSVGAKFLAAFRPLI
HHHCCCCCCEEECCCCCCCCCEECCCEEEEEEEEEEEEECCEEECCCHHHHHHHHHHHHH
EEPLTLIV
HCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]