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The map label for this gene is eno

Identifier: 16125968

GI number: 16125968

Start: 1895879

End: 1897159

Strand: Direct

Name: eno

Synonym: CC_1724

Alternate gene names: 16125968

Gene position: 1895879-1897159 (Clockwise)

Preceding gene: 16125966

Following gene: 16125969

Centisome position: 47.2

GC content: 64.48

Gene sequence:

>1281_bases
ATGACCGAGATCGTCGACATCATCGCCCGCGAAATTCTCGACAGCCGGGGGAACCCGACCGTCGAAGTCGACGTCATTCT
CGAGGACGGCGCGTTCGGTCGCGCCGCAGTGCCCTCGGGCGCCTCGACGGGCGCTCACGAAGCCAACGAAAAGCGTGATG
GCGACAAGGCTCGCTATCTGGGCAAGGGCGTCCAGCAGGCGGTCGACGCCGTCAATGGCGAGATCTTCGACGCGCTGTCG
GGCGTCGACGCCGAAGACCAGCGTCGGGTCGACAACCTGATGATCGAGCTCGACGGTACGCCGAACAAGGCCCGCCTGGG
CGCCAACGCCATCCTCGGCGTCTCGCTGGCCACCGCCAAGGCCGCCGCCGAGTCCGCCGGCCTGCCGCTCTACAAGTATG
TCGGCGGCGTCAACGCCCGCGTGCTGCCGACCCCGATGATGAACATCATCAACGGCGGCGCTCACGCCGACAATCCGATC
GACATCCAGGAGTTCATGATCCTGCCGACCGGCGCCAAGGACTTCCGCGAAGGCCTGCGGATGGGCGCAGAGATCTTCCA
CGCCCTGAAGAAGGCTCTGAAGGACGCGGGTCACAACACCAATGTCGGCGACGAAGGCGGCTTTGCGCCGAACCTGGCGT
CGGCTGAAGCCGCACTCGACTTCATCGTCAAGGCGGGTGAGAAGGCGGGCTACAAGGCCGGTGACGACTTTGTCCTGGGC
CTCGACGTCGCCTCGACCGAGTTCTTCAAGAACGGCAAGTACGAGCTTGAAGGCGAGGGCAAGTCGCTGGATCCGGCCGC
CATGGTCGACTACCTGGCTGGTCTGGTCGCCAAGTTCCCGATCCTGACGATCGAGGACGGCATGGCCGAAGACGACTTCG
ACGGCTGGAAGCTGCTGACCGACACCCTTGGCAAGAAGGTGCAGCTGGTCGGCGACGACCTGTTCGTCACCAACCCCAAG
CGCCTGCAGATGGGCCTGGACAAGGGTCTGGCCAACTCGATCCTGGTGAAGGTCAACCAGATCGGCACGCTCTCGGAAAC
CATCGACGCGGTCGAGCTGGCGCATCGTCACGGTTACACCAGTGTGATGAGCCACCGTTCGGGCGAGACCGAAGACAGCA
CGATCGCGGACCTGGCGGTCGCTCTGAACTGCGGTCAGATCAAGACGGGCTCGCTGGCGCGGTCGGACCGGACGGCCAAG
TACAACCAACTGCTTCGCATCCAGGAAATGCTGGATGATCAGGGCGTCTACGCCGGCCGAGCGGCCCTCAAGGGTCGCTA
A

Upstream 100 bases:

>100_bases
GGGTTATCGACGTTCACGCGGCCTCCCATGGGGAAGTCAAGTTGCGCCTTGGACGCTTGGCGGCTAAGGCTCCCGCGCCT
TTGGCCTTGAGGAGAAGTCC

Downstream 100 bases:

>100_bases
GCAACCCTGGAATAGACGAGACGAAGGGAGCGTGTCCGCGCGAGCGGACACGCTCTTTTCATGTCCGATTCCCGCCGGCG
CGCCTTTGCAGGGGGAGGGA

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTEIVDIIAREILDSRGNPTVEVDVILEDGAFGRAAVPSGASTGAHEANEKRDGDKARYLGKGVQQAVDAVNGEIFDALS
GVDAEDQRRVDNLMIELDGTPNKARLGANAILGVSLATAKAAAESAGLPLYKYVGGVNARVLPTPMMNIINGGAHADNPI
DIQEFMILPTGAKDFREGLRMGAEIFHALKKALKDAGHNTNVGDEGGFAPNLASAEAALDFIVKAGEKAGYKAGDDFVLG
LDVASTEFFKNGKYELEGEGKSLDPAAMVDYLAGLVAKFPILTIEDGMAEDDFDGWKLLTDTLGKKVQLVGDDLFVTNPK
RLQMGLDKGLANSILVKVNQIGTLSETIDAVELAHRHGYTSVMSHRSGETEDSTIADLAVALNCGQIKTGSLARSDRTAK
YNQLLRIQEMLDDQGVYAGRAALKGR

Sequences:

>Translated_426_residues
MTEIVDIIAREILDSRGNPTVEVDVILEDGAFGRAAVPSGASTGAHEANEKRDGDKARYLGKGVQQAVDAVNGEIFDALS
GVDAEDQRRVDNLMIELDGTPNKARLGANAILGVSLATAKAAAESAGLPLYKYVGGVNARVLPTPMMNIINGGAHADNPI
DIQEFMILPTGAKDFREGLRMGAEIFHALKKALKDAGHNTNVGDEGGFAPNLASAEAALDFIVKAGEKAGYKAGDDFVLG
LDVASTEFFKNGKYELEGEGKSLDPAAMVDYLAGLVAKFPILTIEDGMAEDDFDGWKLLTDTLGKKVQLVGDDLFVTNPK
RLQMGLDKGLANSILVKVNQIGTLSETIDAVELAHRHGYTSVMSHRSGETEDSTIADLAVALNCGQIKTGSLARSDRTAK
YNQLLRIQEMLDDQGVYAGRAALKGR
>Mature_425_residues
TEIVDIIAREILDSRGNPTVEVDVILEDGAFGRAAVPSGASTGAHEANEKRDGDKARYLGKGVQQAVDAVNGEIFDALSG
VDAEDQRRVDNLMIELDGTPNKARLGANAILGVSLATAKAAAESAGLPLYKYVGGVNARVLPTPMMNIINGGAHADNPID
IQEFMILPTGAKDFREGLRMGAEIFHALKKALKDAGHNTNVGDEGGFAPNLASAEAALDFIVKAGEKAGYKAGDDFVLGL
DVASTEFFKNGKYELEGEGKSLDPAAMVDYLAGLVAKFPILTIEDGMAEDDFDGWKLLTDTLGKKVQLVGDDLFVTNPKR
LQMGLDKGLANSILVKVNQIGTLSETIDAVELAHRHGYTSVMSHRSGETEDSTIADLAVALNCGQIKTGSLARSDRTAKY
NQLLRIQEMLDDQGVYAGRAALKGR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.3255813953488, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=50.1165501165501, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=51.1627906976744, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=51.1627906976744, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=46.9626168224299, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=25.5882352941176, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=25.5882352941176, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=25.5882352941176, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1789141, Length=431, Percent_Identity=58.9327146171694, Blast_Score=491, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=52.1939953810624, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=52.1939953810624, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=188, Percent_Identity=47.8723404255319, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=50.8120649651972, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=49.537037037037, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=49.3055555555556, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=49.3055555555556, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=49.6519721577726, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=51.1520737327189, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=51.1520737327189, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=51.1520737327189, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=51.1520737327189, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=51.1520737327189, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=51.1520737327189, Blast_Score=386, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_CAUCN (B8GW68)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002517168.1
- ProteinModelPortal:   B8GW68
- SMR:   B8GW68
- GeneID:   7331261
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_01795
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45246; Mature: 45114

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIVDIIAREILDSRGNPTVEVDVILEDGAFGRAAVPSGASTGAHEANEKRDGDKARYL
CHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
GKGVQQAVDAVNGEIFDALSGVDAEDQRRVDNLMIELDGTPNKARLGANAILGVSLATAK
HHHHHHHHHHHCCHHHHHHCCCCCHHHHHHCCEEEEECCCCCHHHHCCCHHEEEHHHHHH
AAAESAGLPLYKYVGGVNARVLPTPMMNIINGGAHADNPIDIQEFMILPTGAKDFREGLR
HHHHHCCCCHHHHHCCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHHH
MGAEIFHALKKALKDAGHNTNVGDEGGFAPNLASAEAALDFIVKAGEKAGYKAGDDFVLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
LDVASTEFFKNGKYELEGEGKSLDPAAMVDYLAGLVAKFPILTIEDGMAEDDFDGWKLLT
EEHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHH
DTLGKKVQLVGDDLFVTNPKRLQMGLDKGLANSILVKVNQIGTLSETIDAVELAHRHGYT
HHHCCEEEEECCCEEECCHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHCCHH
SVMSHRSGETEDSTIADLAVALNCGQIKTGSLARSDRTAKYNQLLRIQEMLDDQGVYAGR
HHHHCCCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECC
AALKGR
HHCCCC
>Mature Secondary Structure 
TEIVDIIAREILDSRGNPTVEVDVILEDGAFGRAAVPSGASTGAHEANEKRDGDKARYL
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
GKGVQQAVDAVNGEIFDALSGVDAEDQRRVDNLMIELDGTPNKARLGANAILGVSLATAK
HHHHHHHHHHHCCHHHHHHCCCCCHHHHHHCCEEEEECCCCCHHHHCCCHHEEEHHHHHH
AAAESAGLPLYKYVGGVNARVLPTPMMNIINGGAHADNPIDIQEFMILPTGAKDFREGLR
HHHHHCCCCHHHHHCCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHHH
MGAEIFHALKKALKDAGHNTNVGDEGGFAPNLASAEAALDFIVKAGEKAGYKAGDDFVLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
LDVASTEFFKNGKYELEGEGKSLDPAAMVDYLAGLVAKFPILTIEDGMAEDDFDGWKLLT
EEHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHH
DTLGKKVQLVGDDLFVTNPKRLQMGLDKGLANSILVKVNQIGTLSETIDAVELAHRHGYT
HHHCCEEEEECCCEEECCHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHCCHH
SVMSHRSGETEDSTIADLAVALNCGQIKTGSLARSDRTAKYNQLLRIQEMLDDQGVYAGR
HHHHCCCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECC
AALKGR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA