Click here to switch to the map view.

The map label for this gene is sigW [H]

Identifier: 16125892

GI number: 16125892

Start: 1821234

End: 1821749

Strand: Direct

Name: sigW [H]

Synonym: CC_1646

Alternate gene names: 16125892

Gene position: 1821234-1821749 (Clockwise)

Preceding gene: 16125891

Following gene: 16125893

Centisome position: 45.34

GC content: 67.64

Gene sequence:

>516_bases
ATGGACGCCGCGCTCGTCAGACAGGCGCGCGCGGGCTCTGAGGTCGCTTTCGCCAGGCTGGTGGCGATCCACGAGAAGCC
GCTGCGGGGCTTTTTACGCAAGAGCGGCTGGATCGACGCCGACGACATCGCCCAGGAAGCCTTCGTGGCGGCCTGGAGTG
ATTTGCCAAGGCTTCGCGATGACGCCGGCTTTCGGGCTTGGCTGTACGGCGTCGCCTGGAAAAAGGCTCTATCCCAGCGT
CGCGGCGCCCAGCGCGCCGCCGCCCGGGACGAGGCCTGGCGTGGCGAGCAGGACCTGGAGGCCGCCGCCGAGGTCGCCGC
CGAAGATCGCCTGGCGCTGGAAACGGCGCTGGCGACTCTGCCGGAAGATCAGCGCGCGTGTGTCACCCTGTGCCTTGGAC
AGGCCTGGTCTCACGCGGAGGTTTCAGACGCTTTGGGCATTCCCTTGGGCACTGTGAAATCGCATGTTACCCGCGGCCGC
GAACGGCTGCTGGCGGTTCTGGGAGGATCTCGATGA

Upstream 100 bases:

>100_bases
GGATGGGCGAGGCGTTCACGATCGTGGCTTTCGTGATGGCCGCCCTGAGCGCCGCGTCGTTGGCCCAGGTGCTGTTCAGC
CGCAAGCGGCCGCGCGACTG

Downstream 100 bases:

>100_bases
CCCCTGATGATCAGCTCTCGGCCTTTCTGGGGGAGCAGCCGGCGACAAGCCGCGCGGATCTGTTCACCGCCGTGGTGATG
CAGAAGATCGAACGCCGCGC

Product: ECF subfamily RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MDAALVRQARAGSEVAFARLVAIHEKPLRGFLRKSGWIDADDIAQEAFVAAWSDLPRLRDDAGFRAWLYGVAWKKALSQR
RGAQRAAARDEAWRGEQDLEAAAEVAAEDRLALETALATLPEDQRACVTLCLGQAWSHAEVSDALGIPLGTVKSHVTRGR
ERLLAVLGGSR

Sequences:

>Translated_171_residues
MDAALVRQARAGSEVAFARLVAIHEKPLRGFLRKSGWIDADDIAQEAFVAAWSDLPRLRDDAGFRAWLYGVAWKKALSQR
RGAQRAAARDEAWRGEQDLEAAAEVAAEDRLALETALATLPEDQRACVTLCLGQAWSHAEVSDALGIPLGTVKSHVTRGR
ERLLAVLGGSR
>Mature_171_residues
MDAALVRQARAGSEVAFARLVAIHEKPLRGFLRKSGWIDADDIAQEAFVAAWSDLPRLRDDAGFRAWLYGVAWKKALSQR
RGAQRAAARDEAWRGEQDLEAAAEVAAEDRLALETALATLPEDQRACVTLCLGQAWSHAEVSDALGIPLGTVKSHVTRGR
ERLLAVLGGSR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=175, Percent_Identity=29.1428571428571, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 18627; Mature: 18627

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAALVRQARAGSEVAFARLVAIHEKPLRGFLRKSGWIDADDIAQEAFVAAWSDLPRLRD
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DAGFRAWLYGVAWKKALSQRRGAQRAAARDEAWRGEQDLEAAAEVAAEDRLALETALATL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
PEDQRACVTLCLGQAWSHAEVSDALGIPLGTVKSHVTRGRERLLAVLGGSR
CCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDAALVRQARAGSEVAFARLVAIHEKPLRGFLRKSGWIDADDIAQEAFVAAWSDLPRLRD
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DAGFRAWLYGVAWKKALSQRRGAQRAAARDEAWRGEQDLEAAAEVAAEDRLALETALATL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
PEDQRACVTLCLGQAWSHAEVSDALGIPLGTVKSHVTRGRERLLAVLGGSR
CCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]