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The map label for this gene is glxR [C]

Identifier: 16125821

GI number: 16125821

Start: 1739691

End: 1740551

Strand: Direct

Name: glxR [C]

Synonym: CC_1574

Alternate gene names: 16125821

Gene position: 1739691-1740551 (Clockwise)

Preceding gene: 16125818

Following gene: 16125824

Centisome position: 43.31

GC content: 68.18

Gene sequence:

>861_bases
ATGAAGACGGCGTTTATCGGCATGGGCGTGATGGGCTTCCCGATGGCCGGTCACCTGAAGGCCGCAGGCCATGAGGTCGC
CGTTTACAATCGCTCCCCCGAAAAGGCCCGTCGCTGGGCTGAAAAGCATGGCGGAGCGGCTTTCGAGACCATCGCTGAGG
CCGTAGCCGGCGCCGAGGTGGTGCTGCTGTGCGTCGGCAACGACGACGATGTCCGCGACCTCGTAGCGCAGGTCCTGCCG
GCCATGGGCGAGGGCGGCGTGATCGTCGACCATACGACCACCTCGGCCAAGGTGGCTCGCGAGATGGCGGCGCTCGCCGC
CCAGAGCGGGAGAGCCTTCGTCGACGCGCCCGTCTCCGGCGGCCAAGCCGGCGCCGAGAGCGGCCAGCTGACCATCATGG
CCGGCGGCGACCAGGCGGCCTATGACCGGGTGCTGCCTGTGATCGAGGTGTACGCCAAGGCCGTGCGGCGCATAGGCGAG
GTGGGCGCGGGCCAGCTAACCAAGATGTGCAACCAGATCGCCATCGCCGGCGTCGTCCAGGGCGTCGCCGAGGCGCTGCA
TTTCGCCAAGCGCGCATGCCTCCCGACCGACGATGTGCTGGCGGCGATCTCGAAGGGCGCGGCCCAGTCGTGGCAGATGG
AGAACCGCTGGCCGACCATGGCGCAGGGCAAGTTCGACTTCGGCTTCGCGGTCGACTGGATGCGCAAGGATCTCGGCATC
GCCCTGGACGAGGCGCGAACCAATGGCGCGAAACTGCCGGCCACGGCTCTGATCGACCAGTTCTACGCCGAGGTCCAGGC
TATGGGCGGGAACCGCTGGGATACCTCCAGCCTGGTGGCTCGCCTCGAGAAGGACGGCTAG

Upstream 100 bases:

>100_bases
CTTAACCTTAAGTCCGCAACCTGCCCAAAGGTCGTTAAGATGGGGTTGCCGGAATGGTCATGGCGCGCGTAATCCCAGCC
TTCTTTTTAGGAGCAGCGGC

Downstream 100 bases:

>100_bases
GCCATAAGCAGATCGACCAGCGACCCCGCGTCCCAGTTTCCGGCGCGGGGCGCGCTCAGGATCGTCGCCTGGTGTTCGAA
CGCGCGCAGCAGGCCCAGGG

Product: hypothetical protein

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKTAFIGMGVMGFPMAGHLKAAGHEVAVYNRSPEKARRWAEKHGGAAFETIAEAVAGAEVVLLCVGNDDDVRDLVAQVLP
AMGEGGVIVDHTTTSAKVAREMAALAAQSGRAFVDAPVSGGQAGAESGQLTIMAGGDQAAYDRVLPVIEVYAKAVRRIGE
VGAGQLTKMCNQIAIAGVVQGVAEALHFAKRACLPTDDVLAAISKGAAQSWQMENRWPTMAQGKFDFGFAVDWMRKDLGI
ALDEARTNGAKLPATALIDQFYAEVQAMGGNRWDTSSLVARLEKDG

Sequences:

>Translated_286_residues
MKTAFIGMGVMGFPMAGHLKAAGHEVAVYNRSPEKARRWAEKHGGAAFETIAEAVAGAEVVLLCVGNDDDVRDLVAQVLP
AMGEGGVIVDHTTTSAKVAREMAALAAQSGRAFVDAPVSGGQAGAESGQLTIMAGGDQAAYDRVLPVIEVYAKAVRRIGE
VGAGQLTKMCNQIAIAGVVQGVAEALHFAKRACLPTDDVLAAISKGAAQSWQMENRWPTMAQGKFDFGFAVDWMRKDLGI
ALDEARTNGAKLPATALIDQFYAEVQAMGGNRWDTSSLVARLEKDG
>Mature_286_residues
MKTAFIGMGVMGFPMAGHLKAAGHEVAVYNRSPEKARRWAEKHGGAAFETIAEAVAGAEVVLLCVGNDDDVRDLVAQVLP
AMGEGGVIVDHTTTSAKVAREMAALAAQSGRAFVDAPVSGGQAGAESGQLTIMAGGDQAAYDRVLPVIEVYAKAVRRIGE
VGAGQLTKMCNQIAIAGVVQGVAEALHFAKRACLPTDDVLAAISKGAAQSWQMENRWPTMAQGKFDFGFAVDWMRKDLGI
ALDEARTNGAKLPATALIDQFYAEVQAMGGNRWDTSSLVARLEKDG

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=27.1477663230241, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=28.4210526315789, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=33.9160839160839, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI145693186, Length=294, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790315, Length=290, Percent_Identity=28.2758620689655, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789092, Length=195, Percent_Identity=35.3846153846154, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788341, Length=265, Percent_Identity=27.9245283018868, Blast_Score=74, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17557316, Length=271, Percent_Identity=25.4612546125461, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=28.4246575342466, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=28.4246575342466, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28574115, Length=287, Percent_Identity=24.390243902439, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30061; Mature: 30061

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAFIGMGVMGFPMAGHLKAAGHEVAVYNRSPEKARRWAEKHGGAAFETIAEAVAGAEV
CCCEEEECCHHCCCCCCCHHHCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHCCCEE
VLLCVGNDDDVRDLVAQVLPAMGEGGVIVDHTTTSAKVAREMAALAAQSGRAFVDAPVSG
EEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCC
GQAGAESGQLTIMAGGDQAAYDRVLPVIEVYAKAVRRIGEVGAGQLTKMCNQIAIAGVVQ
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GVAEALHFAKRACLPTDDVLAAISKGAAQSWQMENRWPTMAQGKFDFGFAVDWMRKDLGI
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
ALDEARTNGAKLPATALIDQFYAEVQAMGGNRWDTSSLVARLEKDG
EEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKTAFIGMGVMGFPMAGHLKAAGHEVAVYNRSPEKARRWAEKHGGAAFETIAEAVAGAEV
CCCEEEECCHHCCCCCCCHHHCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHCCCEE
VLLCVGNDDDVRDLVAQVLPAMGEGGVIVDHTTTSAKVAREMAALAAQSGRAFVDAPVSG
EEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCC
GQAGAESGQLTIMAGGDQAAYDRVLPVIEVYAKAVRRIGEVGAGQLTKMCNQIAIAGVVQ
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GVAEALHFAKRACLPTDDVLAAISKGAAQSWQMENRWPTMAQGKFDFGFAVDWMRKDLGI
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
ALDEARTNGAKLPATALIDQFYAEVQAMGGNRWDTSSLVARLEKDG
EEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA