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The map label for this gene is mrdA [H]

Identifier: 16125793

GI number: 16125793

Start: 1709991

End: 1712009

Strand: Direct

Name: mrdA [H]

Synonym: CC_1546

Alternate gene names: 16125793

Gene position: 1709991-1712009 (Clockwise)

Preceding gene: 16125792

Following gene: 16125794

Centisome position: 42.57

GC content: 68.3

Gene sequence:

>2019_bases
ATGAGCGAACCGTCCATCTTCTTTTTCGAGGTCAACGAGCGTCAGGGGGTCTTCCACCGGCGCGCGTTCCTGCTGGGCGG
TCTTACGGGCCTGGGCCTGCTGACCCTGGGCGGGCGGCTGGCGCAATTGCAGCTGGTCGAGGCTCAGCGCTACCAGAAGC
TCTCCGCCGGCAACCAGTTCAACTACCGCCTGGTGCCGCCGCCGCGCGGTCTGATCATGGATCGCAACGGCGTTTCGCTG
GCGTCCAACCGCCCGAACTTCCGGCTGATGATCAACAAGGAAAAGGGCCTGGAGGCCGAGGCGGTCCTCGACGATCTCTC
CAAGCTGGTTCCGATCGACGGAACCCGCCGGGCGCGGATCCTCAAGGAGCTGGACCGGGCGCCGCGCAAGGCGCCGGTCG
TGGTGATGGAGGACCTCTCCTGGGAGGAGTTCTCGCGAGTCAACATCCGCGCGCCGGAACTGCCCAATGTCACCGCCGAC
ATGGGCGAGGTCCGGGTCTATCCGTTCGGCGGCGCTTTCGCCCACGTGATCGGCTATGTCGCCAAGGTCTCGGACCGTGA
CCTCAAGGCGGCCGAGGGCGACGCCACCCAGGACCAGGAACTGCTGCACCATCCGGGCTTCCGGATCGGCAAGCAGGGCG
TGGAGAAGGCGTTCGACAAGGATCTGCGGGGGCGCGCCGGCGCCACCAAGACCGAGGTCGACTCGGTGGGGCGCGTCGTA
CGGCTCGATCCGGCCGGCGACATTCCGCCCGTGGCCGGCAAGGCGATCCACCTGACCCTGGACGCCGATATCCAGAACCG
CGCCCTCGAGGTGATGGGCGAGGAGAGCGGCGCCATCGTCGTGATGGACTGCCGTAACGGCGACCTGCTGTGCATGGCCT
CCGCGCCGAGCTTCGACGCCAACCGGTTCGTGCGGGGGCTGTCCGGTCCTGAGTACAAGGCCCTGGCCGAGTACGAGCGC
AAGCCGCTGCTCGACAAGTCGATGACCGGCCTGTTTCCGCCCGGCTCGACCTTCAAGCCCACGGTGGGTCTGGCCGCCCT
GGCCGCCGGCATCGATCCCGAGGTCCGGGTCAACTGTCCGGGCAGCTGGTACTATGGCGGTCGGGTGTGGCGTTGCTGGG
AGAAGGGCGGCCACGGCCCCCAGAACATGCACGACGCCATCAAGAATTCTTGTGACGTCTATTTCTACCAGATCTCGCTG
AAGATCGGTCCCGACGCCATCGCCCGCGCCGCCCGCGCCGTCGGCTTTGGCCAGACCTTCGACATCGGCATCCCCGGTCA
GAAGAAGGGCATCGTTCCGGACCGCGAGTGGAAGAAGCGCGCCTTCAAGCGCAATCCGGCCAACCAGATCTGGTTCCCGG
GCGAAACCCCCAGCTACGCCATCGGGCAGGGCGCCTTGACGGTGAATGCGCTGCAACTGGCGGTGATGACGGCCCGGCTG
GCCAACGCCAAGAAGGCGCTGGTTCCCCGGCTCATCAAGTCGATCGGCGACATGGAGCGTCCCGCCGGCTCGGACGTGCC
GGACCTGCCGTTCTCGCCCGAGCACCTGGCCTATGTGCGTAATGGCATGGCCGCCGTGGCCAATGACGTGCGCGGCACCG
CCTACCGCAACAGCCAGCTGGGCCTGGGCGACGTGCAGATGGCCGGCAAGACCGGCACGGCCCAGGTGCGCAGCTACGAC
AAGGGCGTGTCGCGCGACAGCAAGGACGTGCGCTGGCGGCTGAAGGACCACAATCTGTTCGTCGCCTTCGCGCCCTATGA
CGACCCCCGCTATGCGGTGTCGGTCATCATCGAGCACGGTGGCCTGGGCGGGGCCACGGCCGGCGCGCCGCGCGCCCGCG
AGGTGATGCGCGTGGCGCTGCTGAAGGATCCGGAGATCCGCGCCCGCATCGAGCGGCCGGCGCCCTTGCCGGAGGCGGTT
CCCGAGGCCGACATCGAAGGCGCCGCGCCGGAAGATCCGGTCGAAGCGCCGCCGCCCGCGCCCGAAGCGCCCACAACTCC
GCAGGGAGGCCAGACATGA

Upstream 100 bases:

>100_bases
GCGGTGTTCTGGCAGTTCCTGGTGACGGCGCTGCTGTTCCCGTTCGCTCACCGGCTGGTCGATCGCTTCGAGGACGCCGA
CGTGCGGTTCCGGTGAGGCG

Downstream 100 bases:

>100_bases
CCCTGAGCGGCGGCCTTTCCCGGCCCGGCGAACGCGACCGGCCGACCATCAAGTTCATGGAGATCGACTGGACCTTCTGT
CTGTTCCTGTGCCTGATCGC

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MSEPSIFFFEVNERQGVFHRRAFLLGGLTGLGLLTLGGRLAQLQLVEAQRYQKLSAGNQFNYRLVPPPRGLIMDRNGVSL
ASNRPNFRLMINKEKGLEAEAVLDDLSKLVPIDGTRRARILKELDRAPRKAPVVVMEDLSWEEFSRVNIRAPELPNVTAD
MGEVRVYPFGGAFAHVIGYVAKVSDRDLKAAEGDATQDQELLHHPGFRIGKQGVEKAFDKDLRGRAGATKTEVDSVGRVV
RLDPAGDIPPVAGKAIHLTLDADIQNRALEVMGEESGAIVVMDCRNGDLLCMASAPSFDANRFVRGLSGPEYKALAEYER
KPLLDKSMTGLFPPGSTFKPTVGLAALAAGIDPEVRVNCPGSWYYGGRVWRCWEKGGHGPQNMHDAIKNSCDVYFYQISL
KIGPDAIARAARAVGFGQTFDIGIPGQKKGIVPDREWKKRAFKRNPANQIWFPGETPSYAIGQGALTVNALQLAVMTARL
ANAKKALVPRLIKSIGDMERPAGSDVPDLPFSPEHLAYVRNGMAAVANDVRGTAYRNSQLGLGDVQMAGKTGTAQVRSYD
KGVSRDSKDVRWRLKDHNLFVAFAPYDDPRYAVSVIIEHGGLGGATAGAPRAREVMRVALLKDPEIRARIERPAPLPEAV
PEADIEGAAPEDPVEAPPPAPEAPTTPQGGQT

Sequences:

>Translated_672_residues
MSEPSIFFFEVNERQGVFHRRAFLLGGLTGLGLLTLGGRLAQLQLVEAQRYQKLSAGNQFNYRLVPPPRGLIMDRNGVSL
ASNRPNFRLMINKEKGLEAEAVLDDLSKLVPIDGTRRARILKELDRAPRKAPVVVMEDLSWEEFSRVNIRAPELPNVTAD
MGEVRVYPFGGAFAHVIGYVAKVSDRDLKAAEGDATQDQELLHHPGFRIGKQGVEKAFDKDLRGRAGATKTEVDSVGRVV
RLDPAGDIPPVAGKAIHLTLDADIQNRALEVMGEESGAIVVMDCRNGDLLCMASAPSFDANRFVRGLSGPEYKALAEYER
KPLLDKSMTGLFPPGSTFKPTVGLAALAAGIDPEVRVNCPGSWYYGGRVWRCWEKGGHGPQNMHDAIKNSCDVYFYQISL
KIGPDAIARAARAVGFGQTFDIGIPGQKKGIVPDREWKKRAFKRNPANQIWFPGETPSYAIGQGALTVNALQLAVMTARL
ANAKKALVPRLIKSIGDMERPAGSDVPDLPFSPEHLAYVRNGMAAVANDVRGTAYRNSQLGLGDVQMAGKTGTAQVRSYD
KGVSRDSKDVRWRLKDHNLFVAFAPYDDPRYAVSVIIEHGGLGGATAGAPRAREVMRVALLKDPEIRARIERPAPLPEAV
PEADIEGAAPEDPVEAPPPAPEAPTTPQGGQT
>Mature_671_residues
SEPSIFFFEVNERQGVFHRRAFLLGGLTGLGLLTLGGRLAQLQLVEAQRYQKLSAGNQFNYRLVPPPRGLIMDRNGVSLA
SNRPNFRLMINKEKGLEAEAVLDDLSKLVPIDGTRRARILKELDRAPRKAPVVVMEDLSWEEFSRVNIRAPELPNVTADM
GEVRVYPFGGAFAHVIGYVAKVSDRDLKAAEGDATQDQELLHHPGFRIGKQGVEKAFDKDLRGRAGATKTEVDSVGRVVR
LDPAGDIPPVAGKAIHLTLDADIQNRALEVMGEESGAIVVMDCRNGDLLCMASAPSFDANRFVRGLSGPEYKALAEYERK
PLLDKSMTGLFPPGSTFKPTVGLAALAAGIDPEVRVNCPGSWYYGGRVWRCWEKGGHGPQNMHDAIKNSCDVYFYQISLK
IGPDAIARAARAVGFGQTFDIGIPGQKKGIVPDREWKKRAFKRNPANQIWFPGETPSYAIGQGALTVNALQLAVMTARLA
NAKKALVPRLIKSIGDMERPAGSDVPDLPFSPEHLAYVRNGMAAVANDVRGTAYRNSQLGLGDVQMAGKTGTAQVRSYDK
GVSRDSKDVRWRLKDHNLFVAFAPYDDPRYAVSVIIEHGGLGGATAGAPRAREVMRVALLKDPEIRARIERPAPLPEAVP
EADIEGAAPEDPVEAPPPAPEAPTTPQGGQT

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=640, Percent_Identity=32.8125, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1786272, Length=624, Percent_Identity=24.6794871794872, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 73037; Mature: 72906

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPSIFFFEVNERQGVFHRRAFLLGGLTGLGLLTLGGRLAQLQLVEAQRYQKLSAGNQF
CCCCCEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHCCHHEEEEHHHHHHHHHHHCCCCC
NYRLVPPPRGLIMDRNGVSLASNRPNFRLMINKEKGLEAEAVLDDLSKLVPIDGTRRARI
EEEECCCCCCEEECCCCCEECCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHH
LKELDRAPRKAPVVVMEDLSWEEFSRVNIRAPELPNVTADMGEVRVYPFGGAFAHVIGYV
HHHHHCCCCCCCEEEEECCCHHHHCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHH
AKVSDRDLKAAEGDATQDQELLHHPGFRIGKQGVEKAFDKDLRGRAGATKTEVDSVGRVV
HHCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEE
RLDPAGDIPPVAGKAIHLTLDADIQNRALEVMGEESGAIVVMDCRNGDLLCMASAPSFDA
EECCCCCCCCCCCCEEEEEECCCCCHHHHHEECCCCCEEEEEECCCCCEEEEECCCCCCH
NRFVRGLSGPEYKALAEYERKPLLDKSMTGLFPPGSTFKPTVGLAALAAGIDPEVRVNCP
HHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECC
GSWYYGGRVWRCWEKGGHGPQNMHDAIKNSCDVYFYQISLKIGPDAIARAARAVGFGQTF
CCEEECCEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEEEECHHHHHHHHHHHCCCCEE
DIGIPGQKKGIVPDREWKKRAFKRNPANQIWFPGETPSYAIGQGALTVNALQLAVMTARL
ECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCEECCCCEEHHHHHHHHHHHHH
ANAKKALVPRLIKSIGDMERPAGSDVPDLPFSPEHLAYVRNGMAAVANDVRGTAYRNSQL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCEECCCCC
GLGDVQMAGKTGTAQVRSYDKGVSRDSKDVRWRLKDHNLFVAFAPYDDPRYAVSVIIEHG
CCCCEEECCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCCCCEEEEEEEECC
GLGGATAGAPRAREVMRVALLKDPEIRARIERPAPLPEAVPEADIEGAAPEDPVEAPPPA
CCCCCCCCCHHHHHHHHHHHHCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PEAPTTPQGGQT
CCCCCCCCCCCC
>Mature Secondary Structure 
SEPSIFFFEVNERQGVFHRRAFLLGGLTGLGLLTLGGRLAQLQLVEAQRYQKLSAGNQF
CCCCEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHCCHHEEEEHHHHHHHHHHHCCCCC
NYRLVPPPRGLIMDRNGVSLASNRPNFRLMINKEKGLEAEAVLDDLSKLVPIDGTRRARI
EEEECCCCCCEEECCCCCEECCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHH
LKELDRAPRKAPVVVMEDLSWEEFSRVNIRAPELPNVTADMGEVRVYPFGGAFAHVIGYV
HHHHHCCCCCCCEEEEECCCHHHHCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHH
AKVSDRDLKAAEGDATQDQELLHHPGFRIGKQGVEKAFDKDLRGRAGATKTEVDSVGRVV
HHCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEE
RLDPAGDIPPVAGKAIHLTLDADIQNRALEVMGEESGAIVVMDCRNGDLLCMASAPSFDA
EECCCCCCCCCCCCEEEEEECCCCCHHHHHEECCCCCEEEEEECCCCCEEEEECCCCCCH
NRFVRGLSGPEYKALAEYERKPLLDKSMTGLFPPGSTFKPTVGLAALAAGIDPEVRVNCP
HHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECC
GSWYYGGRVWRCWEKGGHGPQNMHDAIKNSCDVYFYQISLKIGPDAIARAARAVGFGQTF
CCEEECCEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEEEECHHHHHHHHHHHCCCCEE
DIGIPGQKKGIVPDREWKKRAFKRNPANQIWFPGETPSYAIGQGALTVNALQLAVMTARL
ECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCEECCCCEEHHHHHHHHHHHHH
ANAKKALVPRLIKSIGDMERPAGSDVPDLPFSPEHLAYVRNGMAAVANDVRGTAYRNSQL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCEECCCCC
GLGDVQMAGKTGTAQVRSYDKGVSRDSKDVRWRLKDHNLFVAFAPYDDPRYAVSVIIEHG
CCCCEEECCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCCCCEEEEEEEECC
GLGGATAGAPRAREVMRVALLKDPEIRARIERPAPLPEAVPEADIEGAAPEDPVEAPPPA
CCCCCCCCCHHHHHHHHHHHHCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PEAPTTPQGGQT
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]