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The map label for this gene is gloA [H]

Identifier: 16125564

GI number: 16125564

Start: 1464205

End: 1464699

Strand: Direct

Name: gloA [H]

Synonym: CC_1315

Alternate gene names: 16125564

Gene position: 1464205-1464699 (Clockwise)

Preceding gene: 16125563

Following gene: 16125565

Centisome position: 36.45

GC content: 62.22

Gene sequence:

>495_bases
TTGCCCCACGCGGGGCTGTGCCTATATCGGCGTGAGGCACCTTCCAGGAGATCCGCCATGCGTTACCTGCACACGATGAT
CCGCGTCCGCGATCTCGACGCCTCGCTGCGGTTCTATTGCAAGGGGCTTGGGCTGCAGGAGATGTATCGCACGGAGAACG
AGAAGGGCCGCTTCACCTTGGTTTTTCTCGCCGCTCCGGAGGACGTGGAGCTCGCCAAGGCGCGTAAGGCTCCCCTGGTT
GAACTCACCTACAACTGGGACGACGAGGAATACGGCGGCGGTCGTAATTTTGGCCACCTGGCCTATCGGGTCGACGACAT
CTACGAGACCTGCCAGCGGCTGATGGACATGGGGGTGACCATCAACCGTCCCCCGCGCGATGGTCACATGGCGTTTGTGC
GCTCACCCGACAATATCTCGATTGAGCTTCTGCAGGACGGCGACCTGCCGCCAGCCGAACCGTGGGTTTCGATGCCGAAC
GTCGGGGCCTGGTAG

Upstream 100 bases:

>100_bases
GATCGTTGAGCGCGGTCGCCATGAGGCCTTGTTGGCGGCCGGCGGCGAATACGCCGCGCTCTGGCGTCGTCAGACGCGGG
ACCGCGAGACGGTTTAGGGT

Downstream 100 bases:

>100_bases
GTCCGCGCACGCGCGGGCGGCCGGATCTTCTGGGTGTCGATCTGGCCGTCCGCTGATCCATATCCAGAGACGCGGGCCGC
GGCCTGTTGGTTCTCCCCGT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MPHAGLCLYRREAPSRRSAMRYLHTMIRVRDLDASLRFYCKGLGLQEMYRTENEKGRFTLVFLAAPEDVELAKARKAPLV
ELTYNWDDEEYGGGRNFGHLAYRVDDIYETCQRLMDMGVTINRPPRDGHMAFVRSPDNISIELLQDGDLPPAEPWVSMPN
VGAW

Sequences:

>Translated_164_residues
MPHAGLCLYRREAPSRRSAMRYLHTMIRVRDLDASLRFYCKGLGLQEMYRTENEKGRFTLVFLAAPEDVELAKARKAPLV
ELTYNWDDEEYGGGRNFGHLAYRVDDIYETCQRLMDMGVTINRPPRDGHMAFVRSPDNISIELLQDGDLPPAEPWVSMPN
VGAW
>Mature_163_residues
PHAGLCLYRREAPSRRSAMRYLHTMIRVRDLDASLRFYCKGLGLQEMYRTENEKGRFTLVFLAAPEDVELAKARKAPLVE
LTYNWDDEEYGGGRNFGHLAYRVDDIYETCQRLMDMGVTINRPPRDGHMAFVRSPDNISIELLQDGDLPPAEPWVSMPNV
GAW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=144, Percent_Identity=37.5, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=37.3134328358209, Blast_Score=91, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323639, Length=154, Percent_Identity=29.8701298701299, Blast_Score=72, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=32.8767123287671, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 18881; Mature: 18750

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHAGLCLYRREAPSRRSAMRYLHTMIRVRDLDASLRFYCKGLGLQEMYRTENEKGRFTL
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHCCCCCCCEEE
VFLAAPEDVELAKARKAPLVELTYNWDDEEYGGGRNFGHLAYRVDDIYETCQRLMDMGVT
EEEECCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHCCCE
INRPPRDGHMAFVRSPDNISIELLQDGDLPPAEPWVSMPNVGAW
ECCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PHAGLCLYRREAPSRRSAMRYLHTMIRVRDLDASLRFYCKGLGLQEMYRTENEKGRFTL
CCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHCCCCCCCEEE
VFLAAPEDVELAKARKAPLVELTYNWDDEEYGGGRNFGHLAYRVDDIYETCQRLMDMGVT
EEEECCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHCCCE
INRPPRDGHMAFVRSPDNISIELLQDGDLPPAEPWVSMPNVGAW
ECCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739; 8021208 [H]