| Definition | Caulobacter crescentus CB15 chromosome, complete genome. |
|---|---|
| Accession | NC_002696 |
| Length | 4,016,947 |
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The map label for this gene is iolD [H]
Identifier: 16125548
GI number: 16125548
Start: 1446324
End: 1448168
Strand: Direct
Name: iolD [H]
Synonym: CC_1299
Alternate gene names: 16125548
Gene position: 1446324-1448168 (Clockwise)
Preceding gene: 16125547
Following gene: 16125549
Centisome position: 36.01
GC content: 69.11
Gene sequence:
>1845_bases ATGGCTCTGGTTCGTCTCACCGCCGCCCAGGCGACCGTACGCTGGCTCGCGGCGCAGCACGTCGAGACGTCGGACGGCGA GGTTCCGTACTTCGCCGGCGTCTGGGCGATCTTCGGCCACGGCAATGTCGCCGGGCTGGGCGAGGCTTTGCACGGCGTTC GCGACGTCCTGCTGACCTATCGCGCCCACAACGAGCAAGGCATGGCCCACGCCGCCATCGCCTTCGCCAAACAGTCCCGG CGCGAGCGGGCGATGGTTTGCACCACCTCGATCGGGCCGGGTGCGACCAACATGGTCACCGCCGCCGCCCTGGCCCATGT GAACCGGCTGCCCGTGCTGTTCCTGCCCGGCGACGTCTACGCCAGCCGCCGGCCAGACCCGGTGCTGCAGCAGATCGAGG ACTTCGGCGACGGCACGATCAGCGCCAATGAATGCTTCAAGCCGGTCTCACGCTATTTCGACCGGATTACGCGGCCCGAA CAGATCCTGGATGCTCTGCCGCGGGCGCTGGCCGTGATGCTGGATCCGGCGACCTGCGGACCGGCTACGCTGGCCTTCTG CCAGGATGTCCAGGCCGAGGCCTTCGACTATCCGCAAGCCTTTTTCGCCAAGCGGGTTTGGCGCATTCGCGCGCCGCAGC CCGATCCTCGGGAGCTCGATGATCTGGTCGGCCTGATCAAGGCCGCTGAGCGTCCGCTCATCGTGGCCGGGGGCGGGGTG CTGTACGCCCGCGCTGAGGCGGCCCTGGCGGACCTGGCGAACGACGCCGGATTGCCGGTCGTCGAGACCCAAGGGGGCAA GGGAAGCCTGGCCTGGGATCATCCCTTGAACCTGGGCGCCGTCGGCGTCACGGGCACGACCGCCTCGGTCGCGGCGGCCG AAGCGGCTGATCTGGTGATCGGTCTGGGCACGCGCCTGCAGGACTTCACAACGGGCTCGCGAGCTCTGTTCGGCGCCGAG GGGCGCAAGCTGGTCCAGGTCAATGTCGCGCCTCATGACGCGCACAAGCATGGAGCGACCAGTGTCGTCGGCGACGCCCT GGCGGTGCTGGAGGCCTTGGCCGAGCGGCTGGACGGCTGGCGTGCGCCGGTCGCCTGGACCGATCAGGCCACCAGCGGCG TGCCGGCCTGGAACGCGATCTTCGACGCCAGCACCAGGGCCGACACGCGGCCTCTGCCCACCGACGCCCAGGTGTTGGGC GCGGTGTGGCGAGCCTCGTCCGACGACAGCGTCGTGGTCTGCGCCGCCGGCGGCCTGCCCGGCGAACTGCACAAGCTTTG GCGCACGCGCATGCCGGGCGGCTACCACGTCGAATACGGCTTCTCCTGCATGGGGTACGAGATCGCCGGCGCGGTCGGCG TGAAAATGGCTGAACCCGACCGCGAGGTGCATGTGATGGTCGGGGACGGCAGCTATCTGATGCTGAACTCCGAACTCGCC ACCTCGGTGATGCTGGGGACCAAGCTGATCGTCACGGTCCTCGACAACCGGGGCTACGGCTGCATCAACCGCCTGCAGGC GGCGACCGGCGGCGCGGCGTTCAACAACTTGCTGGCCGACACGCTGCACGAAACCTTGCCCGACATCGACTTCGCCGCCC ATGCCGCCAGCCTGGGTGCGCGCTCGGAGAAGGTGGGCGATCTGTCGAGCCTGGAAGCCGCGCTTCAGCGCGCCAAGGCC AGCGACCGGACCTATGTCGTCGTCATCGACACCGACCCCATGCCGACGACCGAGGGCGGCGCCTGGTGGGACGTGGCGGT GCCCGAGGTCTCCGTGCGGCCCAGCGTGAACGCCGCGCGCGCCGACTACGACCTGAAGATCAAGCAACAGCGTACAGGGG ACTGA
Upstream 100 bases:
>100_bases ATCGACGATGACGCGGCCGTGACGGCGCTGGCCGCGAAGTTCAAGGTGCTGGTCGACGCCTGGCGTCGCCTCCGAGGCTC CGTGGAAAAGGCCGCTTAGA
Downstream 100 bases:
>100_bases CCCATGACCATCCGTTTCGGCGTGAGCCCCATCGCCTGGATCAACGACGACATGCCCGAGCTGGGCGGGGACACGCCCCT GGAAAGCGTGCTGGCCGACT
Product: iolD protein
Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]
Alternate protein names: THcHDO hydrolase [H]
Number of amino acids: Translated: 614; Mature: 613
Protein sequence:
>614_residues MALVRLTAAQATVRWLAAQHVETSDGEVPYFAGVWAIFGHGNVAGLGEALHGVRDVLLTYRAHNEQGMAHAAIAFAKQSR RERAMVCTTSIGPGATNMVTAAALAHVNRLPVLFLPGDVYASRRPDPVLQQIEDFGDGTISANECFKPVSRYFDRITRPE QILDALPRALAVMLDPATCGPATLAFCQDVQAEAFDYPQAFFAKRVWRIRAPQPDPRELDDLVGLIKAAERPLIVAGGGV LYARAEAALADLANDAGLPVVETQGGKGSLAWDHPLNLGAVGVTGTTASVAAAEAADLVIGLGTRLQDFTTGSRALFGAE GRKLVQVNVAPHDAHKHGATSVVGDALAVLEALAERLDGWRAPVAWTDQATSGVPAWNAIFDASTRADTRPLPTDAQVLG AVWRASSDDSVVVCAAGGLPGELHKLWRTRMPGGYHVEYGFSCMGYEIAGAVGVKMAEPDREVHVMVGDGSYLMLNSELA TSVMLGTKLIVTVLDNRGYGCINRLQAATGGAAFNNLLADTLHETLPDIDFAAHAASLGARSEKVGDLSSLEAALQRAKA SDRTYVVVIDTDPMPTTEGGAWWDVAVPEVSVRPSVNAARADYDLKIKQQRTGD
Sequences:
>Translated_614_residues MALVRLTAAQATVRWLAAQHVETSDGEVPYFAGVWAIFGHGNVAGLGEALHGVRDVLLTYRAHNEQGMAHAAIAFAKQSR RERAMVCTTSIGPGATNMVTAAALAHVNRLPVLFLPGDVYASRRPDPVLQQIEDFGDGTISANECFKPVSRYFDRITRPE QILDALPRALAVMLDPATCGPATLAFCQDVQAEAFDYPQAFFAKRVWRIRAPQPDPRELDDLVGLIKAAERPLIVAGGGV LYARAEAALADLANDAGLPVVETQGGKGSLAWDHPLNLGAVGVTGTTASVAAAEAADLVIGLGTRLQDFTTGSRALFGAE GRKLVQVNVAPHDAHKHGATSVVGDALAVLEALAERLDGWRAPVAWTDQATSGVPAWNAIFDASTRADTRPLPTDAQVLG AVWRASSDDSVVVCAAGGLPGELHKLWRTRMPGGYHVEYGFSCMGYEIAGAVGVKMAEPDREVHVMVGDGSYLMLNSELA TSVMLGTKLIVTVLDNRGYGCINRLQAATGGAAFNNLLADTLHETLPDIDFAAHAASLGARSEKVGDLSSLEAALQRAKA SDRTYVVVIDTDPMPTTEGGAWWDVAVPEVSVRPSVNAARADYDLKIKQQRTGD >Mature_613_residues ALVRLTAAQATVRWLAAQHVETSDGEVPYFAGVWAIFGHGNVAGLGEALHGVRDVLLTYRAHNEQGMAHAAIAFAKQSRR ERAMVCTTSIGPGATNMVTAAALAHVNRLPVLFLPGDVYASRRPDPVLQQIEDFGDGTISANECFKPVSRYFDRITRPEQ ILDALPRALAVMLDPATCGPATLAFCQDVQAEAFDYPQAFFAKRVWRIRAPQPDPRELDDLVGLIKAAERPLIVAGGGVL YARAEAALADLANDAGLPVVETQGGKGSLAWDHPLNLGAVGVTGTTASVAAAEAADLVIGLGTRLQDFTTGSRALFGAEG RKLVQVNVAPHDAHKHGATSVVGDALAVLEALAERLDGWRAPVAWTDQATSGVPAWNAIFDASTRADTRPLPTDAQVLGA VWRASSDDSVVVCAAGGLPGELHKLWRTRMPGGYHVEYGFSCMGYEIAGAVGVKMAEPDREVHVMVGDGSYLMLNSELAT SVMLGTKLIVTVLDNRGYGCINRLQAATGGAAFNNLLADTLHETLPDIDFAAHAASLGARSEKVGDLSSLEAALQRAKAS DRTYVVVIDTDPMPTTEGGAWWDVAVPEVSVRPSVNAARADYDLKIKQQRTGD
Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]
COG id: COG3962
COG function: function code E; Acetolactate synthase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the TPP enzyme family [H]
Homologues:
Organism=Homo sapiens, GI21361361, Length=496, Percent_Identity=23.3870967741935, Blast_Score=77, Evalue=4e-14, Organism=Escherichia coli, GI87081685, Length=555, Percent_Identity=26.8468468468468, Blast_Score=127, Evalue=2e-30, Organism=Escherichia coli, GI1790104, Length=529, Percent_Identity=24.1965973534972, Blast_Score=120, Evalue=2e-28, Organism=Escherichia coli, GI1786717, Length=539, Percent_Identity=26.9016697588126, Blast_Score=119, Evalue=8e-28, Organism=Escherichia coli, GI1787096, Length=495, Percent_Identity=25.0505050505051, Blast_Score=108, Evalue=1e-24, Organism=Escherichia coli, GI1788716, Length=295, Percent_Identity=25.7627118644068, Blast_Score=78, Evalue=2e-15, Organism=Saccharomyces cerevisiae, GI6323755, Length=647, Percent_Identity=24.1112828438949, Blast_Score=126, Evalue=1e-29,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012000 - InterPro: IPR012001 - InterPro: IPR000399 - InterPro: IPR011766 [H]
Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]
EC number: 2.2.1.6 [C]
Molecular weight: Translated: 65107; Mature: 64976
Theoretical pI: Translated: 5.34; Mature: 5.34
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MALVRLTAAQATVRWLAAQHVETSDGEVPYFAGVWAIFGHGNVAGLGEALHGVRDVLLTY CCEEEEHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEECCCCHHHHHHHHHHHHHHHHHH RAHNEQGMAHAAIAFAKQSRRERAMVCTTSIGPGATNMVTAAALAHVNRLPVLFLPGDVY HCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCC ASRRPDPVLQQIEDFGDGTISANECFKPVSRYFDRITRPEQILDALPRALAVMLDPATCG CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCC PATLAFCQDVQAEAFDYPQAFFAKRVWRIRAPQPDPRELDDLVGLIKAAERPLIVAGGGV HHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCE LYARAEAALADLANDAGLPVVETQGGKGSLAWDHPLNLGAVGVTGTTASVAAAEAADLVI EEEHHHHHHHHHHCCCCCCEEECCCCCCCEEECCCCCCCEEEECCCCHHHHHHHHHHEEE GLGTRLQDFTTGSRALFGAEGRKLVQVNVAPHDAHKHGATSVVGDALAVLEALAERLDGW ECCCCHHHCCCCCCEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC RAPVAWTDQATSGVPAWNAIFDASTRADTRPLPTDAQVLGAVWRASSDDSVVVCAAGGLP CCCEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCC GELHKLWRTRMPGGYHVEYGFSCMGYEIAGAVGVKMAEPDREVHVMVGDGSYLMLNSELA HHHHHHHHHCCCCCEEEEECCEEECEEECCCEEEEECCCCCEEEEEECCCCEEEEEHHHH TSVMLGTKLIVTVLDNRGYGCINRLQAATGGAAFNNLLADTLHETLPDIDFAAHAASLGA HHHHHHHEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCC RSEKVGDLSSLEAALQRAKASDRTYVVVIDTDPMPTTEGGAWWDVAVPEVSVRPSVNAAR CHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCCEECCCCCCHHC ADYDLKIKQQRTGD CCCEEEEECCCCCC >Mature Secondary Structure ALVRLTAAQATVRWLAAQHVETSDGEVPYFAGVWAIFGHGNVAGLGEALHGVRDVLLTY CEEEEHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEECCCCHHHHHHHHHHHHHHHHHH RAHNEQGMAHAAIAFAKQSRRERAMVCTTSIGPGATNMVTAAALAHVNRLPVLFLPGDVY HCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCC ASRRPDPVLQQIEDFGDGTISANECFKPVSRYFDRITRPEQILDALPRALAVMLDPATCG CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCC PATLAFCQDVQAEAFDYPQAFFAKRVWRIRAPQPDPRELDDLVGLIKAAERPLIVAGGGV HHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCE LYARAEAALADLANDAGLPVVETQGGKGSLAWDHPLNLGAVGVTGTTASVAAAEAADLVI EEEHHHHHHHHHHCCCCCCEEECCCCCCCEEECCCCCCCEEEECCCCHHHHHHHHHHEEE GLGTRLQDFTTGSRALFGAEGRKLVQVNVAPHDAHKHGATSVVGDALAVLEALAERLDGW ECCCCHHHCCCCCCEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC RAPVAWTDQATSGVPAWNAIFDASTRADTRPLPTDAQVLGAVWRASSDDSVVVCAAGGLP CCCEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCC GELHKLWRTRMPGGYHVEYGFSCMGYEIAGAVGVKMAEPDREVHVMVGDGSYLMLNSELA HHHHHHHHHCCCCCEEEEECCEEECEEECCCEEEEECCCCCEEEEEECCCCEEEEEHHHH TSVMLGTKLIVTVLDNRGYGCINRLQAATGGAAFNNLLADTLHETLPDIDFAAHAASLGA HHHHHHHEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCC RSEKVGDLSSLEAALQRAKASDRTYVVVIDTDPMPTTEGGAWWDVAVPEVSVRPSVNAAR CHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCCEECCCCCCHHC ADYDLKIKQQRTGD CCCEEEEECCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: FAD [C]
Metal ions: Mg2+ [C]
Kcat value (1/min): 4002 [C]
Specific activity: 53
Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]
Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]
Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]
General reaction: C-C bond formation; decarboxylation [C]
Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA