Click here to switch to the map view.

The map label for this gene is alkJ [H]

Identifier: 16125527

GI number: 16125527

Start: 1424133

End: 1425755

Strand: Direct

Name: alkJ [H]

Synonym: CC_1278

Alternate gene names: 16125527

Gene position: 1424133-1425755 (Clockwise)

Preceding gene: 16125526

Following gene: 16125528

Centisome position: 35.45

GC content: 68.88

Gene sequence:

>1623_bases
GTGGGCGACGAAGCGATTCATCTGGGCGACTACGACTACATCGTGGTCGGCGCCGGCTCTGCGGGATGTCTGCTGGCCAA
TCGCCTGTCGGCTGATCCTCGGCGGCGGGTGCTGCTGCTGGAGGCGGGGGGCGACGACAACTGGATCTGGTTTCACGTCC
CGGTCGGCTACCTGTTCGCGATCGGCAATCCCCGCGCCGACTGGATGCTGGAGACCACGCCGCAGGCGGGGCTGGACGGA
CGGGTTCTGGCCTATCCGCGCGGCAAGGTGATCGGCGGCTCATCGGCGATCAACGCCATGATCTATATGCGCGGCCAGGC
TCGGGACTATGACGGCTGGCGACAGCGGGGCCTTGCGGGCTGGGGCTGGCCGGATGTGCTGCCTTACTTTCTGAAGCACG
AAGACCACATCGACCCGCGCGGCGAGCATCATCGGGCCGGCGGCGAGTACCGCGTCGAGCACCCGCGCGTGCGCTGGGAT
GTGCTGGACGCCATCCGTCGCGCGGGTGAGCAGGCCGGTATCGCCCAGGTTGACGACTTCAACGGCGGCGACAACGCGGG
CTCGTCATACTTCCAGGTCAACCAGCGCGCCGGGCGCCGCTGGAGCACAGCCACGGCCTTTCTGAAGCCCGTCCTGTCGC
GTCCCAACCTGCGGCTGGTGAAGGGCGTTGAGGTCGAGCGGCTCATTATCGACGGCAAGCGGGTGCGGGGCCTGCGCGGG
CGTCGGGGTGGAGCGGCGGTGACCGCCAGTGTGTCGGGTGAGTTGATCCTGGCGGCCGGCGCGATCGGTTCGCCCGTGAT
CCTGCAGCGCTCGGGGATCGGGCGCGGCGAGACCCTGTCGCGCGCCGGTGTGGCGATGGTTCACGATCTGCCAGGCGTCG
GGGCCAATCTGCAGGACCATTTGCAGATCCGACCTGTGTTCAAGGTCAGCGGCGTGAGGACGCTGAACACCGACTACGCC
AACCTCTTCCGCCGAGCAGGTATGGGGTTGGACTATCTGCTCCGACGCTCGGGTCCGCTGACCATGGCGCCGTCGCAGTT
GGGGATGTTCTGCCGCTCGGGGCCTGAGTACGAGAGCGCCAATCTGGAGTTCCATTTCCAACCGCTCAGCCTGGACCGCT
GGGGGGAGGGGCTGCACCGGTTCGGCGCGGTGACCGCCAGTGTCTGTAACCTGCGTCCAAGCAGCCGGGGCGCGGTCTCC
CTCTCCGGGCCGGGCCTGGAGCACTCGCCAAGGATCGATCCCAACTACTTGGCGACCGAGGAGGACCGCCGCGTGGCCGT
CGAGTCCCTGAAGTGGGCGCGGCGGATCATGGGGCAGGCGGCCTTGGCGGCCTATGCGCCGGAGGCGTTCCGGCCCGGTC
CGGAGGTGGACGGCGACGCGGCGCTGCTTGCCGCCGCGAAGGCGCTGGCGACGACGATCTTCCACCCGGTCGGTACGGCG
GCCATGGGCGCGGATGGTGATCCCCTGGCGGTGCTGGACGCGCGGCTGCGCGTGCGTGGCGTCGAGGGTCTGCGTGTCAT
CGACGCCTCGGCGATGCCGACCATCACCTCGGGCAACACCAACGCGCCGACTGTCATGATCGCCGAGAAGGGCGCGGCGA
TGATCCTGGAGGATTCCCGCTGA

Upstream 100 bases:

>100_bases
GCTGAAGGTCGTGCGCGAGGCGCTTCGATAGGGGCGGACCGCAAGTCGCTTTCCAGGCGGCCCGGCGGACGGCAGTCTCG
CCCCGGGAGGACGGTCAAGC

Downstream 100 bases:

>100_bases
CGATCCGGTGGGGTTGCGAAGTTAGTTTCGATATGCGTTAAATATTTCGTTAATTGAGAAGGAATGGCGCATGTCCAATC
CCGCGTGCCTGATTTCGGCC

Product: GMC family oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MGDEAIHLGDYDYIVVGAGSAGCLLANRLSADPRRRVLLLEAGGDDNWIWFHVPVGYLFAIGNPRADWMLETTPQAGLDG
RVLAYPRGKVIGGSSAINAMIYMRGQARDYDGWRQRGLAGWGWPDVLPYFLKHEDHIDPRGEHHRAGGEYRVEHPRVRWD
VLDAIRRAGEQAGIAQVDDFNGGDNAGSSYFQVNQRAGRRWSTATAFLKPVLSRPNLRLVKGVEVERLIIDGKRVRGLRG
RRGGAAVTASVSGELILAAGAIGSPVILQRSGIGRGETLSRAGVAMVHDLPGVGANLQDHLQIRPVFKVSGVRTLNTDYA
NLFRRAGMGLDYLLRRSGPLTMAPSQLGMFCRSGPEYESANLEFHFQPLSLDRWGEGLHRFGAVTASVCNLRPSSRGAVS
LSGPGLEHSPRIDPNYLATEEDRRVAVESLKWARRIMGQAALAAYAPEAFRPGPEVDGDAALLAAAKALATTIFHPVGTA
AMGADGDPLAVLDARLRVRGVEGLRVIDASAMPTITSGNTNAPTVMIAEKGAAMILEDSR

Sequences:

>Translated_540_residues
MGDEAIHLGDYDYIVVGAGSAGCLLANRLSADPRRRVLLLEAGGDDNWIWFHVPVGYLFAIGNPRADWMLETTPQAGLDG
RVLAYPRGKVIGGSSAINAMIYMRGQARDYDGWRQRGLAGWGWPDVLPYFLKHEDHIDPRGEHHRAGGEYRVEHPRVRWD
VLDAIRRAGEQAGIAQVDDFNGGDNAGSSYFQVNQRAGRRWSTATAFLKPVLSRPNLRLVKGVEVERLIIDGKRVRGLRG
RRGGAAVTASVSGELILAAGAIGSPVILQRSGIGRGETLSRAGVAMVHDLPGVGANLQDHLQIRPVFKVSGVRTLNTDYA
NLFRRAGMGLDYLLRRSGPLTMAPSQLGMFCRSGPEYESANLEFHFQPLSLDRWGEGLHRFGAVTASVCNLRPSSRGAVS
LSGPGLEHSPRIDPNYLATEEDRRVAVESLKWARRIMGQAALAAYAPEAFRPGPEVDGDAALLAAAKALATTIFHPVGTA
AMGADGDPLAVLDARLRVRGVEGLRVIDASAMPTITSGNTNAPTVMIAEKGAAMILEDSR
>Mature_539_residues
GDEAIHLGDYDYIVVGAGSAGCLLANRLSADPRRRVLLLEAGGDDNWIWFHVPVGYLFAIGNPRADWMLETTPQAGLDGR
VLAYPRGKVIGGSSAINAMIYMRGQARDYDGWRQRGLAGWGWPDVLPYFLKHEDHIDPRGEHHRAGGEYRVEHPRVRWDV
LDAIRRAGEQAGIAQVDDFNGGDNAGSSYFQVNQRAGRRWSTATAFLKPVLSRPNLRLVKGVEVERLIIDGKRVRGLRGR
RGGAAVTASVSGELILAAGAIGSPVILQRSGIGRGETLSRAGVAMVHDLPGVGANLQDHLQIRPVFKVSGVRTLNTDYAN
LFRRAGMGLDYLLRRSGPLTMAPSQLGMFCRSGPEYESANLEFHFQPLSLDRWGEGLHRFGAVTASVCNLRPSSRGAVSL
SGPGLEHSPRIDPNYLATEEDRRVAVESLKWARRIMGQAALAAYAPEAFRPGPEVDGDAALLAAAKALATTIFHPVGTAA
MGADGDPLAVLDARLRVRGVEGLRVIDASAMPTITSGNTNAPTVMIAEKGAAMILEDSR

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=534, Percent_Identity=39.8876404494382, Blast_Score=366, Evalue=1e-101,
Organism=Escherichia coli, GI1786503, Length=535, Percent_Identity=38.1308411214953, Blast_Score=322, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17532301, Length=542, Percent_Identity=33.9483394833948, Blast_Score=314, Evalue=9e-86,
Organism=Drosophila melanogaster, GI24642042, Length=570, Percent_Identity=36.6666666666667, Blast_Score=297, Evalue=2e-80,
Organism=Drosophila melanogaster, GI45549471, Length=570, Percent_Identity=35.7894736842105, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI45551458, Length=570, Percent_Identity=35.7894736842105, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24642048, Length=576, Percent_Identity=36.1111111111111, Blast_Score=295, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24642055, Length=583, Percent_Identity=36.5351629502573, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI17137792, Length=552, Percent_Identity=35.1449275362319, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24642051, Length=569, Percent_Identity=34.9736379613357, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI18859995, Length=567, Percent_Identity=35.978835978836, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24650267, Length=578, Percent_Identity=33.0449826989619, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24642059, Length=572, Percent_Identity=34.965034965035, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI18859993, Length=573, Percent_Identity=32.1116928446771, Blast_Score=254, Evalue=7e-68,
Organism=Drosophila melanogaster, GI24642035, Length=575, Percent_Identity=33.0434782608696, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24642039, Length=576, Percent_Identity=33.1597222222222, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24642037, Length=573, Percent_Identity=33.3333333333333, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=31.0996563573883, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI24642057, Length=589, Percent_Identity=28.6926994906621, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58269; Mature: 58138

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDEAIHLGDYDYIVVGAGSAGCLLANRLSADPRRRVLLLEAGGDDNWIWFHVPVGYLFA
CCCCEEEECCCCEEEEECCCCCEEEHHHCCCCCCCEEEEEEECCCCCEEEEEECCEEEEE
IGNPRADWMLETTPQAGLDGRVLAYPRGKVIGGSSAINAMIYMRGQARDYDGWRQRGLAG
ECCCCCCEEEEECCCCCCCCEEEEECCCCEECCCHHEEEEEEEECCCCCCCHHHHCCCCC
WGWPDVLPYFLKHEDHIDPRGEHHRAGGEYRVEHPRVRWDVLDAIRRAGEQAGIAQVDDF
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCHHCCCEEEECC
NGGDNAGSSYFQVNQRAGRRWSTATAFLKPVLSRPNLRLVKGVEVERLIIDGKRVRGLRG
CCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCHHHCCCCC
RRGGAAVTASVSGELILAAGAIGSPVILQRSGIGRGETLSRAGVAMVHDLPGVGANLQDH
CCCCEEEEEECCCCEEEEECCCCCCEEEEECCCCCCCCHHHCCEEEEECCCCCCCCCHHC
LQIRPVFKVSGVRTLNTDYANLFRRAGMGLDYLLRRSGPLTMAPSQLGMFCRSGPEYESA
EEEEEEEEECCEEEECCHHHHHHHHHCCCHHHHHHCCCCCEECHHHHHHHHHCCCCCCCC
NLEFHFQPLSLDRWGEGLHRFGAVTASVCNLRPSSRGAVSLSGPGLEHSPRIDPNYLATE
CEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
EDRRVAVESLKWARRIMGQAALAAYAPEAFRPGPEVDGDAALLAAAKALATTIFHPVGTA
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
AMGADGDPLAVLDARLRVRGVEGLRVIDASAMPTITSGNTNAPTVMIAEKGAAMILEDSR
HCCCCCCCEEEEECEEEEECCCCEEEEECCCCCEEECCCCCCCEEEEECCCCEEEEECCC
>Mature Secondary Structure 
GDEAIHLGDYDYIVVGAGSAGCLLANRLSADPRRRVLLLEAGGDDNWIWFHVPVGYLFA
CCCEEEECCCCEEEEECCCCCEEEHHHCCCCCCCEEEEEEECCCCCEEEEEECCEEEEE
IGNPRADWMLETTPQAGLDGRVLAYPRGKVIGGSSAINAMIYMRGQARDYDGWRQRGLAG
ECCCCCCEEEEECCCCCCCCEEEEECCCCEECCCHHEEEEEEEECCCCCCCHHHHCCCCC
WGWPDVLPYFLKHEDHIDPRGEHHRAGGEYRVEHPRVRWDVLDAIRRAGEQAGIAQVDDF
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCHHCCCEEEECC
NGGDNAGSSYFQVNQRAGRRWSTATAFLKPVLSRPNLRLVKGVEVERLIIDGKRVRGLRG
CCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCHHHCCCCC
RRGGAAVTASVSGELILAAGAIGSPVILQRSGIGRGETLSRAGVAMVHDLPGVGANLQDH
CCCCEEEEEECCCCEEEEECCCCCCEEEEECCCCCCCCHHHCCEEEEECCCCCCCCCHHC
LQIRPVFKVSGVRTLNTDYANLFRRAGMGLDYLLRRSGPLTMAPSQLGMFCRSGPEYESA
EEEEEEEEECCEEEECCHHHHHHHHHCCCHHHHHHCCCCCEECHHHHHHHHHCCCCCCCC
NLEFHFQPLSLDRWGEGLHRFGAVTASVCNLRPSSRGAVSLSGPGLEHSPRIDPNYLATE
CEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
EDRRVAVESLKWARRIMGQAALAAYAPEAFRPGPEVDGDAALLAAAKALATTIFHPVGTA
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
AMGADGDPLAVLDARLRVRGVEGLRVIDASAMPTITSGNTNAPTVMIAEKGAAMILEDSR
HCCCCCCCEEEEECEEEEECCCCEEEEECCCCCEEECCCCCCCEEEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]